电超滤浸提土壤养分的研究 Ⅰ.石灰性土壤的电超滤特性

石灰性土壤的电超滤(EUF)特性主要是浸提时因需控制电流而必须大大降低电压。在一定范围内,降压程度与土壤 CaCO_3%有密切关系。控流限额愈低,电压需成比例地下降,但土壤EUF-值减小不多。电流控制在一定值时,离子的解吸总量亦为定值。控流主要控制了各石灰性土壤解吸/溶解份额特大的 Ca+Mg 量近于定值,对于含量小的其他养分离子则并无多大影响,仍可区辨各土壤间的差别。通过冬小麦盆栽试验和有关化学测试法等方面的相关研究,表明采用国际通用的5g 土样和控电流方法测定 EUF-K 和-P 等有效养分,对于石灰性土壤也是可行的,但其EUF-(Ca+Mg)值则毫无植物营养有效性的意义。土壤 EUF 值只是一种指数,并非真正的解吸离子量。石灰性土壤 EUF-K 值因浸提时降压控流而略低,80℃时5min 内解吸的钾不一定是非交换性钾。由于 Ca-P 和 CaCO_3在高温高电压时浸出较多,故石灰性土壤 EUF-P 和-(Ca+Mg)的80℃值/20℃值之比,与酸性土壤或非石灰性土壤相比要大得多。     

Direct and Residual Contributions of Symbiotic Nitrogen Fixation by Legumes to the Yield and Nitrogen Uptake of Maize (Zea mays L.) in the Nigerian Savannah

Field experiments were conducted to determine the direct and residual contributions of legumes to the yield and nitrogen (N) uptake of maize during the wet seasons of 1994 and 1995 at the University Farm, Abubakar Tafawa Balewa University, Bauchi, Nigeria, located in the Northern Guinea savannah of Nigeria. Nodulating soybean, lablab, green gram and black gram contributed to the yield and N uptake of maize either intercropped with the legumes or grown after legumes as a sole crop. Direct transfer of N from the nodulating soybean, lablab, green gram and black gram to the intercropped maize was 24.9–28.1, 23.8–29.2, 19.7–22.1 and 18.4–18.6 kg N ha^–1, respectively. However, the transfer of residual N from these legumes to the succeeding maize crop was 18.4–20.0, 19.5–29.9, 12.0–13.7 and 9.3–10.3 kg N ha^–1, respectively. Four years of continuous lablab cropping resulted in yields and N uptake of the succeeding maize crop grown without fertilizer N that were comparable to the yields and N uptake of the succeeding maize crop supplied with 40–45 kg N ha^–1 and grown after 4 years of continuous sorghum cropping. It may therefore be concluded that nodulating soybean, lablab, green gram and black gram may be either intercropped or grown in rotation with cereals in order to economize the use of fertilizer N for maize production in the Nigerian savannah.     

Research on abalone (Haliotis midae L.) cultivation in South Africa

Although abalone fisheries in South Africa have existed since 1949, cultivation started by successful spawning of captured specimens in 1981 to produce spat and juveniles. Twelve abalone farms, with an estimated investment of US$12 million, have since been established on the coast of South Africa, with a projected production of 500–800 tons. While Haliotis midae could reach a maximum size of about 200 mm shell length at an age of over 30 years in the wild, farm production is concentrating on an average size of 100 mm after 5 years. Growth rates of 0.08–4.5% body weight day ^?1 for abalone of 10–17 mm shell length have been found under stimulated farming conditions on formulated diets, while the corresponding feed conversion ratio (FCR) was 0.9–2.4. Growth rate, FCR and protein efficiency ratio decline at water temperatures above 20 °C. Anaesthetics, for example magnesium sulphate, are used to prevent injury during removal from tanks. Prostrate diatoms, such as Cocconeis sublittoralis (Hendey), Amphora proteoides (Hustedt) and Achnanthes brevipes (Agardh) are preferred after the larval stage (5–7 days, depending on temperature) are finished. South Africa abalone growers prefer formulated feeds in pellet form, which is convenience and cost favourable to farm operations and management. Although fish meal has been found to be the most suitable protein source for inclusion in formulated diets, plant proteins such as soy bean meal, cottonseed meal and sunflower meal presented good growth and apparent protein digestibility. The parasite sabellid polychaete, Terebrasabella heterouncinata, recently named by Australian and American researchers, is indigenous to a variety of South African intertidal molluscs and impedes growth by causing irritation beneath the mantle in abalone. Prevention to some degree is possible by high standards of hygiene and husbandry of abalone in the tanks, but new techniques to control large infestations now include ultrasound. In spite of a reputation for toughness, abalone meat frozen immediately after shucking is tender relative to red meat. The success of abalone farming in South Africa has been as a result of a high degree of cooperation between the private sector and government‐backed research institutions, and it is anticipated that this collaboration will continue.     

中密度纤维板砂光机的改进及降耗

笔者分析了中密度纤维板砂光机消耗较高、质量较差的原因，提出了改进措施。实施结果表明，砂光机运行正常，砂光产量和质量同步提高，消耗和成本下降，污染减轻。     

延边地区紫苏保护技术的研究

紫苏是延边地区的重要经济作物之一．但目前随着种植年限的延续，开始大量发生虫害、病害以及草害等，从而导致减产、降效，严重影响苏子生产．研究结果表明，延边紫苏地适宜土壤处理的除草剂为敌草胺与扑草净的混剂，使用剂量为3kg／hm^2 5kg／hm^2，一年生杂草除草效果可达到90％以上；茎叶处理除草剂为拿捕净(12．5％，1．0kg／hm^2)、稳杀得(35％，1．0kg／hm^2)、精禾草克(5％，1．0kg／hm^2)；紫苏的主要病害为锈病，对紫苏叶片质量影响较大；主要食粒虫害(待定)对产量的影响较大．     

辣椒夏季品比试验报告

为了考察我公司新推广品种及新配组合在高温伏旱状况下的适应性，我们于2003年5月7日在公司试验基地进行了品种对比试验。结果表明新推广品种中有九个品种或组合为极耐热或耐热，一个品种耐热性稍差。新配组合中部分表现优良，有推广前景。     

集雨限灌对旱作马铃薯产量及水分利用效率的影响

在大田条件下研究了集雨有限补偿灌溉对旱区马铃薯产量及水分利用效率的影响。结果表明,补灌能显著提高旱作马铃薯的产量、水分利用效率和经济系数。与对照相比,补灌处理产量增幅在3.99%~21.21%之间,苗期补灌产量高于薯块膨大期补灌,苗期补灌45 mm具有超补偿效应,补灌90 mm具有高补偿效应。低定额补灌有利于水分利用效率和灌水利用效率的提高,且苗期补灌高于薯块膨大期补灌。补灌明显降低了薯块小薯率而提高了大薯率和中薯率,另外单株薯重也提高了,单株薯重与大薯率对马铃薯的产量贡献最大。综合分析认为苗期补灌45 mm和90 mm是最优补灌方案和备选方案。     

真核表达NDV F基因重组质粒的构建及其在CEF细胞中的转染

通过PCR方法自含NDVZF基因的克隆质粒中扩增NDVF基因 ,将其与真核表达载体pcDNA3..1/V5_His_TOPO重组。经核酸内切酶酶切 ,阳性克隆鉴定准确无误 ,核酸序列测定结果与原始基因比较 ,同源性大于 99% ,启始密码和终止密码未出现变异 ,将该重组质粒命名为pcDNANDVZF。将pcDNANDVZF在脂质体作用下转染CEF细胞 ,用间接免疫荧光试验检测 ,结果证明pcDNANDVZF可在CEF细胞中大量表达F蛋白。     

猪肺炎支原体及其生物学研究进展

猪肺炎支原体是引起猪支气管肺炎的主要病原 ,严重危害着养猪事业的发展。虽然猪肺炎支原体的结构简单 ,但由于它对营养要求较高 ,故培养难度大 ,也是人们不能对其深入研究的一个主要原因。近年来利用克隆技术 ,对猪肺炎支原体从基因水平上进行了多种研究 ,已经取得了一些可喜成果。文章从它的生物学特性、荚膜结构、膜蛋白研究以及基因水平的研究等方面进行了综合性论述 ,并简明地指出了目前研究中存在的问题以及未来展望