水稻旱作对其生长量和经济产量的影响

1998～1999年，研究了水稻旱作条件下，不同生育时期受水分胁迫后对作物生长量的影响。结果表明：不同指标对水分胁迫时期的敏感性不一，植株高度对抽穗期水分胁迫最敏感；单株绿叶面积和单株地上部干重对幼穗分化后期水分胁迫最敏感；根系干重和根冠比对幼穗分化前期水分胁迫最敏感；幼穗分化后期水分胁迫对经济产量影响程度最大，无效分蘖期水分胁迫对产量影响程度最小。     

The basic mechanism of increased microvascular permeability

The increased microvascular permeability appears mainly in venule during inflammation, shock, and burns. Endothelial cells play an important role in venule permeability enhancement. There are two kinds of pathway for macromolecule extravasation. One is paracellular pathway and another is transcellular pathway, which are related to the formation of endothelial gap or transcellular openings seperately. The alteration of intercellular related protein, such as occludin, claudin, zona occludens (ZO), junctional adhesion molecule (JAM), VE cadherin, catenin, integrin, etc, and the alteration of endothelial cytoskeleton, such as rearrangement of actin filament, formation of stress fiber and focal adhesion, etc, involve in the pathogenesis of increased microvascular permeability.     

Effect of lansoprazole on gastric ulceration in rats

AIM:To investigate the effect of lansoprazole on gastric ulceration in rats. METHODS:Using the gastric ulcer model induced by hemorrhagic shock, restraint water-immersion stress and pylorus-ligature, the protective effect of lansoprazole (iv) on gastric ulceration was observed. RESULTS:Pretreatment with lansoprazole (7.5-60 mg/kg) significantly inhibited the formation of gastric ulcer in the three models in a dose-dependent manner. The autiulcer efficacy of lansoprazole was similar to that of omeprazole in the equal dose, but stronger than that of omeprazole for ulcer induced by water-immersion stress.CONCLUSION:The intravenously administered lansoprazole inhibited formation of experimental gastric ulcer in rats.     

Effect of Jiawei Sini Decoction on glucocorticoid receptor in thymocytes of chronic psychological stress rats

AIM: To observe the effect of Jiawei Sini Decoction (JWSND) on glucocorticoid receptor (GCR) in thymocytes of chronic psychological stress rats. METHODS: The rats were randomly divided into 4 groups, control group (C), model group (M), group treated by JWSND C₁, group treated by ginsenosides C₂. The number of thymocyte GCR sites and the GCR nuclear thanslocation rate were detected by radioimmunoassay. RESULTS: Compared with the control group, in the model group, the thymocyte weight index lowered significantly ( P<0.05 ), and the GCR nuclear thanslocation rate was increased significantly ( P<0.01 ), but the number of thymocyte GCR sites was unchanged. Compared with the model group, thymus gland weight indexes of C₁ and C₂ were increased significantly ( P<0.05 ), while the GCR nuclear thanslocation rate lowered significantly ( P<0.01 ). Moreover, no significant difference was found in all indexes between C₁ and control group. CONCLUSION: The inhibitory effect of glucocorticoid on the thymus could be significantly reversed by JWSND via suppressing the thanslocation of GCR from cytoplasm to nucleus in chronic psychological stress rats.     

Male-to-female aggression in cage-housed common pheasants (Phasianus colchicus) during the breeding season was not related to male plasma testosterone level

1. The aim of this study was to investigate if male-to-female aggression of common pheasants in the course of the breeding season was related to the concentration of plasma testosterone and/or other biochemical plasma indicators in male pheasants housed in breeding cages. The influence of season on the concentration of testosterone and biochemical indicators was also investigated.

2. Males were divided into non-aggressive and aggressive groups during the breeding season based on ethological evaluation. At the beginning, in the middle and at the end of the breeding season, a blood sample was taken from all males on the same day and the concentration of selected biochemical indicators and the total circulating testosterone in the plasma were determined.

3. Male-to-female aggression during the breeding season of pheasants was not influenced by the total plasma testosterone of males.

4. The concentration of total plasma testosterone in males decreased gradually during the breeding season.

5. Male-to-female aggression of pheasants did not have a significant effect on any of the assessed biochemical indicators.

6. The influence of the breeding season affected the activities of alanine aminotransferase and aspartate aminotransferase as well as the concentrations of glucose, magnesium, potassium and chloride in the blood plasma of cage-housed male pheasants.     

Spermatic and oxidative profile of domestic cat (Felis catus) epididymal sperm subjected to different cooling times (24, 48 and 72 hours)

Cooling stored epididymal samples for several days allows facilities to transport and process genetic material post‐mortem. Improvements to this practice allow the preservation of sperm from domestic cats, which are the ideal study model for wild felids. However, the modifications in spermatic features and the oxidative profile are not fully understood in cats. This information is necessary for the development of biotechniques, such as new extenders for cryopreservation. Therefore, the purpose of this study was to evaluate the spermatic and oxidative profile in samples from the epididymal cauda of domestic cats cooled at 5°C for 24, 48 and 72 hr. Spermatozoa were collected from the epididymis cauda. Evaluations consisted of computer‐assisted sperm analysis (CASA), plasma membrane integrity (eosin/nigrosin), acrosome integrity (fast green/rose bengal), sperm morphology, sperm DNA integrity (toluidine blue), mitochondrial activity (3′3 diaminobenzidine), activity of the antioxidant enzymes glutathione peroxidase (GPx) and superoxide dismutase (SOD), measurement of lipid peroxidation (TBARS) and protein oxidation. A decrease in sperm motility parameters was observed after 72 hr of cooling (i.e. total and progressive) with a higher percentage of minor (37.7 ± 6.3%) and total defects (53.4 ± 6.3%). Additionally, a decrease in high mitochondrial activity (Class I: 16.6 ± 2.2%) occurred after 72 hr. The decrease in motility rates after a long cooling time probably was caused by the increase in sperm abnormalities. A long cooling time causes cold shock and mitochondrial exhaustion, but there was no observed change with the oxidative stress condition. Therefore, cat epididymal sperm stored at 5°C appear to maintain a high quality for up to 48 hr of cooling time.     

外施苹果酸对紫色小白菜生长、生理与品质的影响

【目的】探究外施苹果酸对紫色小白菜生长、生理与品质的影响,筛选最适的外施苹果酸质量浓度,为紫色小白菜的优质培育以及高产栽培奠定理论基础。【方法】以‘京研'紫色小白菜为试验材料,采用水培技术,设置外施苹果酸0 mg/L(对照,CK)、0.5 mg/L(T1)、1.5 mg/L(T2)、3.0 mg/L(T3)、5.0 mg/L(T4)、10.0 mg/L(T5)共6个处理,研究不同质量浓度外施苹果酸对紫色小白菜叶片和根系形态学指标、根系显微结构及相关品质指标的影响。【结果】(1)随苹果酸质量浓度的增大,紫色小白菜叶片和根系形态指标均呈现先上升后下降的趋势,其中T1、T2处理显著优于对照,叶片表面积、体积较大,且可促进植株根系增长、面积增大、侧根增粗,而T5处理因苹果酸质量浓度过高阻碍了根系伸展。(2)根系显微结构观察发现,T1、T2、T3处理紫色小白菜新生侧根根尖1 cm处直径均显著高于对照,维管束和木质部区域直径增大,有助于运输更多的营养物质和水分。T5处理根系皮层细胞层数较少,细胞体积较大且排列疏松。(3)紫色小白菜叶片可溶性蛋白和维生素C含量随处理时间延长逐渐增高,随苹果酸质量浓度的增大呈先上升后下降的趋势,以T2处理最优。紫色小白菜叶片可溶性糖和总有机酸含量9 d时整体下降,且随苹果酸质量浓度的增大呈先上升后下降趋势,T1、T2处理显著高于对照。(4)苹果酸、丙二酸、琥珀酸和酒石酸含量随苹果酸质量浓度的增加呈先上升后下降的趋势,而柠檬酸含量呈先下降后上升的趋势。T1、T2、T3处理苹果酸、丙二酸、琥珀酸含量较高,对照、T4、T5处理柠檬酸含量较高。【结论】外施苹果酸能够影响紫色小白菜的生长及生理状况,施用适宜质量浓度(1.5 mg/L)的苹果酸可有效促进紫色小白菜地上部和根系生长,提高苹果酸、丙二酸、琥珀酸含量,提升植株营养品质。     

Improving cereal yield forecasts in Europe – The impact of weather extremes

The impact of extreme events (such as prolonged droughts, heat waves, cold shocks and frost) is poorly represented by most of the existing yield forecasting systems. Two new model-based approaches that account for the impact of extreme weather events on crop production are presented as a way to improve yield forecasts, both based on the Crop Growth Monitoring System (CGMS) of the European Commission. A first approach includes simple relations – consistent with the degree of complexity of the most generic crop simulators – to explicitly model the impact of these events on leaf development and yield formation. A second approach is a hybrid system which adds selected agro-climatic indicators (accounting for drought and cold/heat stress) to the previous one. The new proposed methods, together with the CGMS-standard approach and a system exclusively based on selected agro-climatic indicators, were evaluated in a comparative fashion for their forecasting reliability. The four systems were assessed for the main micro- and macro-thermal cereal crops grown in highly productive European countries. The workflow included the statistical post-processing of model outputs aggregated at national level with historical series (1995–2013) of official yields, followed by a cross-validation for forecasting events triggered at flowering, maturity and at an intermediate stage. With the system based on agro-climatic indicators, satisfactory performances were limited to microthermal crops grown in Mediterranean environments (i.e. crop production systems mainly driven by rainfall distribution). Compared to CGMS-standard system, the newly proposed approaches increased the forecasting reliability in 94% of the combinations crop × country × forecasting moment. In particular, the explicit simulation of the impact of extreme events explained a large part of the inter-annual variability (up to +44% for spring barley in Poland), while the addition of agro-climatic indicators to the workflow mostly added accuracy to an already satisfactory forecasting system.     

颗粒日粮中添加柠条对滩羊生长性能、血液生化指标、瘤胃发酵及羊肉品质的影响

[目的]研究颗粒日粮中添加柠条对滩羊生长性能、血液生化指标、瘤胃发酵及羊肉品质的影响。[方法]选择体重相近的健康去势滩羊20只,随机分成两组,每组10只;对照组饲喂含有10%菊花粕的颗粒日粮,试验组饲喂含有6%柠条和4%菊花粕的颗粒日粮;开展周期为50天的育肥试验,测定滩羊的生长性能指标;育肥试验结束后,测定滩羊血液生化指标;屠宰后测定滩羊的屠宰性能指标,并取瘤胃液测定瘤胃发酵指标;取背腰最长肌,测定粗脂肪、粗蛋白、氨基酸含量以及脂肪酸组成。[结果]试验组和对照组滩羊的初始体重和终末体重无显著（P>0.05）差异;试验组平均日增重较对照组降低10.84%,差异不显著（P>0.05）;对照组平均日采食量显著（P<0.05）高于试验组;两组试验羊料肉比相同。试验组滩羊血清中的总蛋白含量显著（P<0.05）高于对照组,其他血液生化指标无显著（P>0.05）差异。对照组滩羊屠宰率比试验组高1.59%,差异不显著（P>0.05）;两组试验羊肝脏重、肝脏指数、心脏和肺脏重、心脏和肺脏指数差异不显著（P>0.05）。试验组滩羊瘤胃液中丁酸浓度显著（P<0.05）高于对照组,异戊酸浓度显著（P<0.05）低于对照组。对照组和试验组滩羊背腰最长肌中分别检测出18种和20种脂肪酸,粗蛋白含量分别为17.51%和20.92%,粗脂肪含量分别为3.22%和3.89%,总氨基酸含量分别为13.25%和14.09%。[结论]颗粒日粮中加入柠条对滩羊生长性能、血液生化指标和屠宰性能影响不明显,但可改善瘤胃发酵,丰富羊肉中脂肪酸组成及含量,增加肉中粗蛋白、粗脂肪和氨基酸含量。     

Environmental stress response limits microbial necromass contributions to soil organic carbon

The majority of dead organic material enters the soil carbon pool following initial incorporation into microbial biomass. The decomposition of microbial necromass carbon (C) is, therefore, an important process governing the balance between terrestrial and atmospheric C pools. We tested how abiotic stress (drought), biotic interactions (invertebrate grazing) and physical disturbance influence the biochemistry (C:N ratio and calcium oxalate production) of living fungal cells, and the subsequent stabilization of fungal-derived C after senescence. We traced the fate of ¹³C-labeled necromass from ‘stressed’ and ‘unstressed’ fungi into living soil microbes, dissolved organic carbon (DOC), total soil carbon and respired CO₂. All stressors stimulated the production of calcium oxalate crystals and enhanced the C:N ratios of living fungal mycelia, leading to the formation of ‘recalcitrant’ necromass. Although we were unable to detect consistent effects of stress on the mineralization rates of fungal necromass, a greater proportion of the non-stressed (labile) fungal necromass C was stabilised in soil. Our finding is consistent with the emerging understanding that recalcitrant material is entirely decomposed within soil, but incorporated less efficiently into living microbial biomass and, ultimately, into stable SOC.