Vertebral column deformity with curved cross-stitch vertebrae in Norwegian seawater-farmed Atlantic salmon,Salmo salar L.

Pathological changes in the vertebral column of farmed Atlantic salmon in Norway have been reported since the 1990s. Based on the characteristic radiographic findings, we here present a vertebral column deformity named “curved cross-stitch vertebrae” that mainly affects the middle aspect of the vertebral column. Sixty fish, from the west/northwest coast of mid-Norway, were sampled at slaughter and examined by radiography, computed tomography (CT), necropsy, macrophotography, and histology. The vertebral deformities were radiographically graded as mild, moderate, or marked. The main differences between these grades of changes were defined by increased curving of the peripheries of endplates, reduced intervertebral spaces, and vertical displacement of the vertebrae. The curved rims of endplates were located peripheral to a continuous and approximately circular borderline. The CT studies revealed small, multifocal, hypo-attenuating, round to crescent-shaped areas in the notochord, compatible with the presence of gas. Additionally, histology revealed that the axial parts of endplates had circular zones with perforations, through which either notochordal tissue prolapsed into the vertebrae or vascularized fibrochondroid proliferations extended from the vertebrae into the notochord. Inflammation was present in many vertebral bodies. To the best of our knowledge, this is the first report of gas in the notochord of fish.     

Decellularization of Extracellular Matrix from Equine Skeletal Muscle

Equine represents an attractive animal model for musculoskeletal tissue diseases, exhibiting much similarity to the injuries that occur in humans. Cell therapy and tissue bioengineering have been widely used as a therapeutic alternative by regenerative medicine in musculoskeletal diseases. Thus, the aim of this study was to produce an acellular biomaterial of equine skeletal muscle and to evaluate its effectiveness in supporting the in vitro culture of equine induced pluripotency stem cells (iPSCs). Biceps femoris samples were frozen at −20°C for 4 days and incubated in 1% sodium dodecyl sulfate (SDS), 5 mM EDTA + 50 mM Tris and 1% Triton X-100; the effectiveness of the decellularization was evaluated by the absence of remnant nuclei (histological and 4′,6-diamidino-2-phenylindole [DAPI] analysis), preservation of extracellular matrix (ECM) proteins (immunofluorescence and immunohistochemistry) and organization of ECM ultrastructure (scanning electron microscopy). Decellularized samples were recellularized with iPSCs at the concentration of 50,000 cells/cm² and cultured in vitro for 9 days, and the presence of the cells in the biomaterial was evaluated by histological analysis and presence of nuclei. Decellularized biomaterial showed absence of remnant nuclei and muscle fibers, as well as the preservation of ECM architecture, vascular network and proteins, laminin, fibronectin, elastin, collagen III and IV. After cellularization, iPSC nuclei were present at 9 days after incubation, indicating the decellularized biomaterial-supported iPSC survival. It is concluded that the ECM biomaterial produced from the decellularized equine skeletal muscle has potential for iPSC adhesion, representing a promising biomaterial for regenerative medicine in the therapy of musculoskeletal diseases.     

Adaptation characteristics of mitochondria in plateau pikas at different altitudes

AIM: To investigate the adaptive characteristics of mitochondria in plateau pikas at different altitudes. METHODS: According to the altitudes of the capture area, plateau pikas were divided into 4 300 m group (Mado Star Sea, n=6) and 2 900 m group (Laoji Mountain South, n=6). The red blood cells and hemoglobin of the animals in 2 groups were measured. The levels of mitochondrial oxidative phosphorylation (OXPHOS) and fatty acid oxidation (FAO) in the liver and skeletal muscle, and mitochondrial H₂O₂ production level in the skeletal muscle were determined by high-resolution mitochondrial respirometer. RESULTS: The hemoglobin of the plateau pika in 4 300 m group was significantly lower than those in 2 900 m group. The liver state 4 respiration rate of mitochondrial respiratory chain complex I (CI-LEAK), mitochondrial respiratory chain complex I state 3 respiration rate (CI-OXPHOS) and respiratory chain complex I+Ⅱ state 3 respiration rate(CI+Ⅱ-OXPHOS), electron transfer capacity of respiratory chain complex I+Ⅱ (CI+Ⅱ-ETS), the electron transport capacity of the respiratory chain complex Ⅱ (CⅡ-ETS) in 2 900 m group were increased, and the coupling efficiency was decreased compared with 4 300 m group (P<0.05). The skeletal muscle CI-LEAK,CI+Ⅱ-OXPHOS and CI+Ⅱ-ETS were higher than those in 4 300 m group. Mitochondrial state 4 respiration rate with palmitic acid carnitine and malate as substrates (FAO-LEAK) of liver and skeletal muscle in 2 900 m group were increased compared with 4 300 m group. The skeletal muscle with palmitic acid carnitine as a substrate for fatty acid metabolism state 3 respiratory rate (FAO-OXPHOS) was increased in 2 900 m group compared with 4 300 m group. The H₂O₂/O₂ flow ratio (ΔH₂O₂) in 2 900 m group was lower in state 4 respiration rate of mitochondrial respiratory chain complex I (LEAK), state 3 of mitochondrial respiratory chain complex I (OXPHOS), state 3 of mitochondrial respiratory chain complex I+Ⅱ (OXPHOS*) and electron transfer capacity of respiratory chain complex I+Ⅱ (ETS) than 4 300 m group. CONCLUSION: Plateau pikas with different altitudes have different mitochondrial adaptation characteristics.     

Use of 25-hydroxyvitamin D3 in diets for sows: A review

Dietary supplementation with 25-hydroxyvitamin D₃ (25OHD₃), as an alternative source of vitamin D, is becoming increasingly popular due to its commercialization and more efficient absorbability. The addition of 25OHD₃ rather than its precursor vitamin D₃ can circumvent the 25-hydroxylation reaction in the liver, indicating that supplementation of 25OHD₃ can rapidly improve the circulating vitamin D status of animals. Emerging experiments have reported that maternal 25OHD₃ supplementation could increase sow performances and birth outcomes and promote circulating vitamin D status of sows and their offspring. Increased milk fat content was observed in many experiments; however, others demonstrated that adding 25OHD₃ to lactating sow diets increased the contents of milk protein and lactose. Although an inconsistency between the results of different experiments exists, these studies suggested that maternal 25OHD₃ supplementation could alter milk composition via its effects on the mammary gland. Previous studies have demonstrated that adding 25OHD₃ to sow diets could improve the mRNA expressions of insulin-induced gene 1 (INSIG1) and sterol regulatory element-binding protein 1 (SREBP1) in the mammary gland cells from milk and increase the mRNA expressions of acetyl-CoA carboxylase α (ACCα) and fatty acid synthase (FAS) in the mammary gland tissue. Maternal 25OHD₃ supplementation promotes skeletal muscle development of piglets before and after parturition, and improves bone properties including bone density and bone breaking force in lactating sows and their piglets. Interestingly, 25OHD₃ supplementation in sow diets could improve neonatal bone development via regulation of milk fatty acid composition related to bone metabolism and mineralization. In this review, we also discuss the effects of adding 25OHD₃ to sow diets on the gut bacterial metabolites of suckling piglets, and propose that butyrate production may be associated with bone health. Therefore, to better understand the nutritional functions of maternal 25OHD₃ supplementation, this paper reviews advances in the studies of 25OHD₃ for sow nutrition and provides references for practical application.     

Complications after two transphyseal bridging techniques for treatment of angular limb deformities of the distal radius in 568 Thoroughbred yearlings

Reasons for performing study: Surgical correction of carpal angular limb deformities by growth retardation is commonly undertaken with a screws and tension band wire loop technique (S&W) or a single transphyseal screw (STS). This study compares complications after S&W and STS bridging in the distal radius of Thoroughbred yearlings. Objective: To compare the prevalence of complications serious enough to require follow‐up radiographs following either S&W or STS surgery for growth manipulation in the distal radius of Thoroughbred yearlings. Methods: Medical records and radiographs from Thoroughbred yearlings (age range 261–457 days) treated for carpal angular limb deformities at a single hospital over 2 years were reviewed. Each of the techniques was used exclusively during a single year. The complication threshold criterion for inclusion was the need for nonroutine radiographs of the operated site anytime after implant insertion or removal. Results: Of 568 horses, 253 received S&W and 315 received STS. Horses were of similar age at the time of surgery for STS and S&W. Single transphyseal screws were left in place for a significantly shorter amount of time (16 days). Sex, the limb(s) treated and medial vs. lateral placement were not significantly different between techniques. Complications included physitis post implant removal, metaphyseal collapse post implant removal, infection, overcorrection and seroma formation severe enough to require radiography. Physitis and metaphyseal collapse occurred significantly more frequently with STS compared with S&W. Infection, overcorrection and seromas were not significantly different between techniques. Conclusion: The STS and S&W techniques are both viable treatment options for correction of carpal angular limb deformities. However, horses treated with the STS technique have a significantly increased risk of developing physitis or metaphyseal collapse. Potential relevance: Horses treated with STS bridging have a significantly increased risk of developing the post correction complications of moderate to severe physitis and metaphyseal collapse compared with horses treated with S&W bridging.     

Effects of Water Extract from Different Parts of Betelnut on Physiological Indexes in Mice

The experiment was aimed to explore the influence of water extract from fresh betelnut's different parts on physiological indexes in mice.Four-week-old and seven-week-old SPF KM mice at the weight of (18±2)g and (35±2)g were used in the experiment,respectively.48 four-week-old mice with half male and half female were randomly divided into four groups.These mice were intragastricly administered with distilled water (control group,CK),water extract from betelnut,water extract from fresh betelnut's peels and seeds once a day and were observed for 14 days to determine the survival rate,feed intake,water intake and body temperature.36 seven-week-old male mice were randomly divided into 4 groups to determine deformity rate of sperm in mice,and the intragastric administration was the same as above.The results showed that the survival rate of betelnut's seeds were 0 at the sixth day,and other treatments had no death;Compared with CK,the experimental treatments' feed intake and water intake were reduced,and they were the lowest in the betelnut's seeds treatment and had significant difference with other treatments (P<0.05),the betelnut treatment took the second place.All the treatments' body temperature were lower than CK,and the peels treatment's body temperature was the most stable one and was similar to CK(P>0.05),the body temperature in the betelnut's seeds treatment was the lowest and had significant difference with other treatments (P<0.05).Compared to CK,the other 3 treatments all increased sperm deformity rate of mice,the highest sperm deformity rate went to betelnut's seeds treatment (P<0.01),and betelnut treatment took the second place (P<0.01),then was the peels treatment (P<0.05),moreover,there were extremely significant difference among these 3 treatments (P<0.01).The results showed that the water extract from fresh betelnut's seeds had the greatest influence on mice,then was the water extract from betelnut,while the impact of water extract from fresh betelnut's peels was less.     

Effect of acupuncture on mitophagy of skeletal muscle in rats with exercise-induced skeletal muscle damage

AIM: The effect of acupuncture on mitophagy-related protein expression in skeletal muscle of rats after heavy-load exercise was investigated to explore the role of acupuncture in the repairment of exercise-induced skeletal muscle damage. METHODS: Male adult Sprague-Dawley rats (n=128) were randomly divided into 4 groups:control (C, n=8) group, exercise (E, n=40) group, acupuncture (A, n=40) group, and exercise and acupuncture (EA, n=40) group. The rats in E group and EA group performed an eccentric exercise, and the rats in A group and EA group immediately after exercise received acupuncture treatment. The rats in the latter 3 groups were further divided into 0 h, 12 h, 24 h, 48 h and 72 h sub-groups (n=8), and soleus muscle was collected at each time point. The transmission electron microscopy was used to observe the ultrastructural changes of the mitochondria in skeletal muscle. The content of citrate synthase (CS) was measured by ELISA. The protein expression of skeletal muscle PTEN-induced putative kinase 1 (PINK1), parkin and microtubule-associated protein 1 light chain 3 (LC3) was determined by Western blot. RESULTS: After the heavy-load exercise, the mitochondria swelled and accumulated under cell membrane. The number of mitophagosomes was increased, and the content of CS was significantly decreased (P<0.05). The expression of PINK1, parkin and LC3 was significantly elevated (P<0.05). However, the acupuncture intervention after exercise promoted the recovery of mitochondrial ultrastructure, attenuated mitophagolysosome formation, maintained CS content and down-regulated the expression of PINK1, parkin and LC3 (P<0.05). CONCLUSION: Heavy-load exercise causes the damages of mitochondrial structure and function in the skeletal muscle and activates PINK1/parkin pathway to induce excessive occurrence of mitophagy. Acupuncture intervention after exercise is able to alleviate the damage of mitochondria in the skeletal muscle through decreasing the expression of mitochondrial outer membrane protein PINK1, reducing the recruitment of downstream cytoplasmic protein parkin, thereby affecting the combination of LC3 and mitochondria to inhibit the overactivation of mitophagy.     

Farriery for the foal: A review part 1: Basic trimming

The extensive nature of this topic warrants this review paper to be divided into two parts: ‘Routine basic trimming in foals’ and ‘Therapeutic farriery in foals’. Hoof care in the first few months of life is serious business and should never be taken lightly. Good farriery is vital for the development of the hoof capsule and the conformation of the limb. Management of the feet and limbs during the juvenile period will often dictate the success of the foal as a sales yearling or mature sound athlete. Overall hoof care of a foal is often a joint venture between the veterinarian and the farrier. Part 1 of this paper will outline the concept of a footcare programme, examination of the foal's feet and limbs along with what constitutes good basic farriery to properly apply the trim. It will also emphasise the importance of maintaining a good veterinarian-farrier relationship - the farrier being responsible for basic trimming with veterinary oversight when necessary, and if orthopaedic disorders develop, the farrier will have significant input with therapeutic farriery.