冰冻切片免疫酶和免疫荧光技术快速检测REV抗原

以禽网状内皮组织增生病病毒(reticuloendotheliosis virus,REV)的单克隆抗体11B154作一抗,建立了在冰冻切片中快速检测REV的免疫酶(IHC)和免疫荧光技术(Mc-IFA)。运用两种技术对人工接种REV的肉种鸡和疑似REV田间病例的肝脏、脾脏、腺胃、心脏、骨髓等器官的组织进行了检测,并同病毒分离方法进行了敏感性和特异性的比较。结果表明:在人工接种的肉种鸡中,采用IHC、Mc-IFA和病毒分离方法结果一致,均为阳性;在送检的疑似RE的24份病料中,用IHC检测出阳性率51.7%(13/24),Mc-IFA检测出阳性率62.5%(15/24),其中13份存在交叉;病毒分离的阳性率为37.5%(9/24),阳性数包括在13份IHC阳性病料中。由此表明冰冻切片免疫酶和免疫荧光技术都可用于临床快速检测,其中免疫荧光可用于早期的粗检,而免疫酶可用于细胞内抗原定位。     

黄河兰州段沉积物对DAP吸附特性研究

[目的]更好地掌握持久性有机毒物在黄河中上游的迁移转化及其归趋。[方法]利用固相萃取-高效液相色谱和紫外分光光度法讨论黄河兰州段沉积物对水体中邻苯二甲酸正二戊酯的吸附行为及各种因素如温度、离子强度、pH值和有机物含量对其吸附行为影响。[结果]结果表明,吸附等温线呈现较为明显的"S"形,采用Freundlich方程拟合的相关性比Langmuir方程好,表明为多分子层吸附;当温度升高时,吸附量增大,吸附反应为吸热过程;离子强度,水体环境pH和沉积物中有机质含量都对其有一定的影响。[结论]该研究为持久性有机毒物在黄河中上游的迁移转化及其归趋提供了一定的理论基础。     

1例黄牛剖腹产手术的过程与分析

介绍了1例黄牛剖腹产手术的过程,分析了其中的不当之处,并提出意见和建议.     

砼深受弯构件及圆形截面构件受剪承载力

结合 GBJ10 - 89混凝土结构设计规范修订课题 ,提出了混凝土深受弯构件和圆形截面偏压构件抗剪承载力的计算方法 .这些方法与试验资料吻合良好 ,并与矩形截面一般受弯构件的计算方法在一定程度上得到了统一 .     

籼稻体细胞胚胎发生形态鉴别的研究

以籼稻品种秋桂矮１１发芽种子的功芽基部为外植体，以Ｎ６培养基附加２，４－Ｄ２ｍｇ／Ｌ和脯氨酸０－４０ｍｍｏｌ／Ｌ诱导愈伤组织，对愈伤组织进行了立体解剖，扫描电镜观察和ＧＭＡ塑料薄切片观察。结果表明，愈伤组织可分为胚性、中间型和非胚性３大类，胚性愈伤组织与非胚性愈伤组织在外观特征和内部结构方面都存在显著差异，前者明显分化成６类细胞，其中包括传递细胞；后者分化程度低，且没有观察到传递细胞，中间型愈伤组     

T形截面三维编织复合材料细观结构分析及弯曲性能预测

根据矩形组合截面三维编织复合材料四步法编织原理,分析了T形截面编织物的纱线运动规律;按照载纱器的水平运动规律确定了纱线的空间运动轨迹,并用控制体积法建立T形截面交接区域的特殊细观结构模型.在假设纱线截面为椭圆形等理想状态下,建立了编织工艺参数之间的数学关系.运用弯曲刚度合成法预测出T形截面三维编织复合材料的弯曲性能,用MatLab编写了弯曲模量的计算程序.结果表明:弯曲模量随编织角的增大而减小,随纤维体积含量的增加而增大,三维五向T形截面梁的弯曲性能优于三维四向的.最后将预测结果与实验结果进行比较分析,结果证实用弯曲刚度合成法预测T形截面三维编织复合材料的弯曲性能是可靠的.     

New taxa in Aspergillus section Usti

Based on phylogenetic analysis of sequence data, Aspergillus section Usti includes 21 species, inclucing two teleomorphic species Aspergillus heterothallicus (= Emericella heterothallica) and Fennellia monodii. Aspergillus germanicus sp. nov. was isolated from indoor air in Germany. This species has identical ITS sequences with A. insuetusCBS 119.27, but is clearly distinct from that species based on β-tubulin and calmodulin sequence data. This species is unable to grow at 37 °C, similarly to A. keveii and A. insuetus. Aspergillus carlsbadensis sp. nov. was isolated from the Carlsbad Caverns National Park in New Mexico. This taxon is related to, but distinct from a clade including A. calidoustus, A. pseudodeflectus, A. insuetus and A. keveii on all trees. This species is also unable to grow at 37 °C, and acid production was not observed on CREA. Aspergillus californicus sp. nov. is proposed for an isolate from chamise chaparral (Adenostoma fasciculatum) in California. It is related to a clade including A. subsessilis and A. kassunensis on all trees. This species grew well at 37 °C, and acid production was not observed on CREA. The strain CBS 504.65 from soil in Turkey showed to be clearly distinct from the A. deflectus ex-type strain, indicating that this isolate represents a distinct species in this section. We propose the name A. turkensis sp. nov. for this taxon. This species grew, although rather restrictedly at 37 °C, and acid production was not observed on CREA. Isolates from stored maize, South Africa, as a culture contaminant of Bipolaris sorokiniana from indoor air in Finland proved to be related to, but different from A. ustus and A. puniceus. The taxon is proposed as the new species A. pseudoustus. Although supported only by low bootstrap values, F. monodii was found to belong to section Usti based on phylogenetic analysis of either loci BLAST searches to the GenBank database also resulted in closest hits from section Usti. This species obviously does not belong to the Fennellia genus, instead it is a member of the Emericella genus. However, in accordance with the guidelines of the Amsterdam Declaration on fungal nomenclature (Hawksworth et al. 2011), and based on phylogenetic and physiological evidence, we propose the new combination Aspergillus monodii comb. nov. for this taxon. Species assigned to section Usti can be assigned to three chemical groups based on the extrolites. Aspergillus ustus, A. granulosus and A. puniceus produced ustic acid, while A. ustus and A. puniceus also produced austocystins and versicolorins. In the second chemical group, A. pseudodeflectus produced drimans in common with the other species in this group, and also several unique unknown compounds. Aspergillus calidoustus isolates produced drimans and ophiobolins in common with A. insuetus and A. keveii, but also produced austins. Aspergillus insuetus isolates also produced pergillin while A. keveii isolates produced nidulol. In the third chemical group, E. heterothallica has been reported to produce emethallicins, 5'-hydroxyaveranthin, emeheterone, emesterones, 5'-hydroxyaveranthin.     

冻马哈鱼片/段加工工艺的研究

冷冻马哈鱼片/段,原料从国外进口,加工成品后再销往美国、欧盟、俄罗斯等地区.原料一般有去头去脏,或者带头去脏2种.对于一直从事淡水鱼片(鮰鱼片)加工的企业来说,其积累了丰富的经验,来料加工的这种模式在生产设备的需求和工艺上和其他鱼片加工区别不大,除了在原料的供应和产品质量控制的关注点不同外,利用公司运行的体系,可以将产品的质量控制在最佳范围之内.但在加工过程中,从原料验收到成品形成全过程都可能受到不同程度的污染,因此在加工过程中应严格按照相关操作规范进行生产加工,确保产品质量.     

3种多年生荞麦植株顶端茎段的可溶性糖含量

为了对3种多年生荞麦植物中的23个居群的菜用品质进行评价,采用蒽酮法测定各植株顶端不同季节及不同茎段的可溶性糖含量。结果表明:1)多年生荞麦顶端幼叶的可溶性糖含量最高,并随节位数降低而减少;2)春季含量最高,与夏秋两季的差异水平达极显著,夏秋两季之间差异不显著;3)3种荞麦中,大野荞、毛野荞的可溶性糖含量较高,差异不显著,硬枝万年荞可溶性糖含量最少,与大野荞的差异显著,与毛野荞的差异不显著。结论:筛选出可溶性糖含量较高的8个多年生荞麦居群,其顶端3个茎节的可溶性糖含量为10.23%~15.37%,春季适宜采摘顶端3~4节食用,夏秋季采摘顶端1~2节食用。     

木材鉴定在刑事侦查案件中的应用

以微观特征为基础,以宏观特征为补充验证,采用徒手切片法对一桩刑侦案件涉案木质器械残留木屑进行了木材鉴定,结果判定为广泛分布于安徽各地的蝶形花科(Papilionaceae)黄檀属(Dalbergia L.F.)的黄檀(D. hupeana Hance),俗名不知春.并从理论上系统介绍了木材鉴定在刑侦案件中的应用方法、步骤及注意事项.