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991.
992.
Bulked Segregant Analysis to Detect QTL Related to Heat Tolerance in Rice (Oryza sativa L.) Using SSR Markers 总被引:3,自引:0,他引:3
ZHANG Gui-lian CHEN Li-yun XIAO Guo-ying XIAO Ying-hui CHEN Xin-bo ZHANG Shun-tang 《中国农业科学(英文版)》2009,8(4):482-487
The study was undertaken to assess the genetic effect of quantitative trait loci (QTLs) conferring heat tolerance at flowering stage in rice. A population consisting of 279 F2 individuals from the cross between 996, a heat tolerant cultivar and 4628, a heat-sensitive cultivar, was analyzed for their segregation pattern of the difference of seed set rate under optimal temperature condition and high temperature condition. The difference of seed set rate under optimal temperature condition and high temperature condition showed normal distribution, indicating the polygenic control over the trait. To identify main effect of QTL for heat tolerance, the parents were surveyed with 200 primer pairs of simple sequence repeats (SSR). The parental survey revealed 30% polymorphism between parents. In order to detect the main QTL association with heat tolerance, a strategy of combining the DNA pooling from selected segregants and genotyping was adopted. The association of putative markers identified based on DNA pooling from selected segregants was established by single marker analysis (SMA). The results of SMA revealed that SSR markers, RM3735 on chromosome 4 and RM3586 on chromosome 3 showed significant association with heat tolerance respectively, accounted for 17 and 3% of the total variation respectively. The heat tolerance during flowering stage in rice was controlled by multiple gene. The SSR markers, RM3735 on chromosome 4 and RM3586 on chromosome 3 showed significant association with heat tolerance respectively, accounted for 17 and 3% of the total variation respectively. The two genetic loci, especially for RM3735 on chromosome 4, can be used in marker-assistant-selected method in heat tolerance breeding in rice. 相似文献
993.
Integrated pest management in field vegetable crops in northern Europe — with focus on two key pests
Improvements in (1) insecticide application, (2) supervised control, and (3) pest forecasting systems have each helped to reduce considerably the amounts of insecticides required to control fly, caterpillar and aphid infestations in vegetable crops in northern Europe. By growing plants that are partially resistant to certain major pests, it is now possible to apply even less insecticide than the dose recommended for the crop. In crops where only small amounts of insecticides are applied, natural predators should prevent large increases in pest insect populations and natural parasitoids should reduce the numbers of pest insects entering subsequent generations. The possible impact of introducing transgenic plants and the use of physical (crop covers), cultural (crop rotation, undersowing) and microbial (e.g. fungi, bacteria and nematodes) methods of control are also discussed. The withdrawal of certain insecticides, as a result of environmental and commercial pressures, means that some crops may soon be without appropriate insecticides for controlling one or more of the major pest species. Whether such systems will be sustainable, remains to be seen. 相似文献
994.
995.
采用观察法和问卷调查法对贵州师范大学宝山校区绿地进行使用状况评价,主要包括植物造景分析、园林配套设施情况、行为活动分析,行为痕迹分析。总结该校园绿地的优缺点,并提出合理的改建方案。 相似文献
996.
论述了UEA在施工中使其结构承重与防水功能合一,可不铺贴卷材而达到结构的防水目的从而给建筑设计和建筑防水施工带来很大的进步。 相似文献
997.
植物病原丝状真菌寄生性与RGS蛋白的关系研究 总被引:1,自引:0,他引:1
以49个全基因组序列已经公布的真菌为研究对象,通过OrthoVenn2同源基因簇比对、BLASTp比对和关键词搜索3种方法对G蛋白信号调控因子(regulators of G-protein signaling,RGS)同源基因进行比对分析,并结合SMART进行保守结构域分析,结果发现,49个真菌中共有229个RGS蛋白,每种真菌中所含有RGS蛋白的数量范围为3~9个,且死体营养型病原菌和半活体营养型病原菌的RGS蛋白数量高于活体营养型病原菌;根据RGS蛋白中保守结构域进行分类,可以划分为DEP-RGS、RGS-TM、PXA-RGS-PX、RGS、RGS-PAS-PAC、TM-RGS等6种,其中,具有RGS-PAS-PAC和TM-RGS这2种特殊保守结构域的RGS蛋白主要集中在半活体营养型病原菌和死体营养型病原菌;进一步对229个RGS蛋白进行遗传关系分析,发现具有同种保守结构域的RGS蛋白亲缘关系较近。上述研究结果表明植物病原丝状真菌的寄生性与RGS蛋白数量和种类之间存在着一定的关联性。 相似文献
998.
M. Hühn 《Journal of Agronomy and Crop Science》1999,182(2):89-98
Based on several simplifying assumptions, a stochastic approach was developed which allows an estimation of the effects of nonregular spatial patterns of the distribution of individual plants on yield per area (F). In this approach, two random variables were attached to each plant: single plant yield (E) and individual space per plant (A). The latter was estimated by the area of Thiessen polygons. Yield per area was calculated theoretically by the expectation of the ratio E / A. Appropriate approximations of this expectation depend on the means (ē and ā), coefficients of variation (vE and vA) of E and A and their correlation (rEA). Yield per area can be decomposed into two additive terms: the first term gives the commonly used estimate ē/ā— or h(ā)/ā if a functional relationship between E and A is assumed: E = h(A). In this study, the two relationships E = k1 + k2 · ln A and E = A/(k3 + k4A) were used (with appropriately chosen constants k1, k2, k3, and k4). The second term in the decomposition of F can be interpreted as the effect of variable individual plant spaces on yield per area. In this paper, all theoretical concepts and results were applied to 17 experimental data sets of three cultivars of winter oilseed rape (Brassica napus L.). Single plant yields (E) and individual plant areas (A) were positively correlated with correlation coefficients from 0.64 up to 0.91. The ranges for both coefficients of variation were similar: 0.27 ≤ vE ≤ 0.65 and 0.28 ≤ vA ≤ 0.59. One obtains no significant differences in the goodness-of-fit for both tested relationships between E and A although the logarithmic relationship seems to be slightly superior. For only three data sets one obtains negative values for the percentage of the second term in the decomposition of F. This indicates an overestimation of yield per area by the commonly used estimates h(ā)/ā and ē/ā, respectively. These overestimations, however, are less than 5 %. In all other cases with positive values for the second term the yield per area is underestimated by the common estimates. For almost all data sets, however, the percentages of F which are explained by the common estimates are much larger than 90 %. 相似文献
999.
以油菜为材料,研究线粒体nad6基因在种子发育中的表达。结果表明,nad6基因在叶片中的表达很低,在种子发育过程中各阶段均有较高表达,并随种子发育进程而有加强的趋势。推测线粒体nad6基因可能除与呼吸作用有关外还有其他功能 相似文献
1000.
刘芳 《中南林业科技大学学报(自然科学版)》1999,(1)
对配合使用酸法、碱法以及加热法提取刺槐叶蛋白的工艺及其工艺条件进行了研究.结果表明,刺槐叶蛋白的提取条件为:常温下料液比为1∶5,pH值为7;刺槐叶蛋白的分离条件为:温度为60℃时,沉淀分离得到叶绿体蛋白,调节pH值为10时分离出细胞质叶蛋白I,调节pH值为2时分离出细胞质叶蛋白质I;刺槐叶蛋白产品的干燥条件为:50~70℃条件下真空干燥.采用此工艺提取刺槐叶蛋白得率为4.7%,提取物中蛋白质含量为50%~80%,原料总氮提取率为75.9%. 相似文献