CARDIOVASCULAR AND GASTROINTESTINAL RADIOGRAPHIC CONTRAST STUDIES IN THE HORSESHOE CRAB (LIMULUS POLYPHEMUS)

Survey and contrast radiographic studies of the cardiovascular and gastrointestinal systems of the American horseshoe crab (Limulus polyphemus) were undertaken using conventional and flouroscopic techniques. Normal gastrointestinal and cardiovascular structures were difficult to resolve on survey radiographs, but were well visualized with gastrointestinal follow through studies and angiography, respectively. All three contrast agents (iohexol, Gastrografin, and barium sulfate) used for the gastrointestinal studies varied minimally in their radiographic quality and transit times, and all appeared to be safe for use in the horseshoe crab.     

real-time PCR将成为分子生物学研究的重要检测技术

近年来,real-tim e PCR技术作为一种敏感、快速、简便、重复性好的检测技术已广泛出现在生物学研究领域。real-tim e polym erase chain reaction(RT-PCR)是Kary M ullis在二十世纪八十年代在PCR的基础上发展革新的一项新技术,它能使小量DN A片段扩增一百万倍。这项技术推动了分子生物学技术的发展,简便的操作不仅可应用于普通实验,还可用于人类基因组工程。根据2003年调查的406位科学工作者,48%的研究人员近年来正在使用这项技术。随着使用这项技术的人数增多,各类相关的论文也相继增多。real-tim e PCR将会影响PCR技术的发展,并…     

荧光定量PCR检测小鼠卵母细胞中Mad2 mRNA的表达

目的:应用SYBR G reen I染料法建立检测Mad2 mRNA表达的实时荧光定量PCR的方法,并探讨小鼠卵母细胞减数分裂成熟中Mad2 mRNA的表达。方法:采用实时荧光定量PCR的方法,以SYBR G reen I为荧光染料,梯度稀释的重组质粒为模板制作标准曲线,并以此方法分析了小鼠卵母细胞减数分裂成熟过程中Mad2 mRNA表达的动态变化。结果:建立了实时荧光定量检测方法分析小鼠卵母细胞中Mad2 mRNA的表达,该方法灵敏、特异,扩增效率接近100%。进行了标准曲线的制作,以及对目的基因Mad2转录水平的绝对定量。结论:实时荧光定量PCR是检测基因表达水平的理想选择,在小鼠卵母细胞减数分裂成熟过程中Mad2 mRNA表达存在动态变化。     

茶树新品系福选9号的观察研究

为观察鉴定茶树新品系福选9号在重庆市巴南区的适应性及表现,以福鼎大白茶为对照品种,于2002-2004年进行了品比试验。结果表明,福选9号表现出如下特性:(1)春芽萌动特早,较对照提早11～13d,春茶产量较高。(2)育芽能力强,生长势旺盛,秋梢休止期较对照晚5～7d,全年生育期较对照长16～20d,平均单产高20.5%。(3)抗逆性强,耐寒性和抗旱性与对照相当,抗病虫性稍强。(4)发芽整齐度高,适宜机械化作业。(5)制绿茶品质优良,适制性强。     

虾蛄蛋白浆对河蟹摄食和生长影响的研究

分别用虾蛄蛋白浆替代饲料中2%和4%的鱼粉,并以乌贼膏为对照,研究其对河蟹摄食和生长的影响。结果表明,饲料中添加虾蛄蛋白浆对河蟹的诱食作用与乌贼膏相当,但摄食量显著高于乌贼膏组。此外,虾蛄蛋白浆还具有明显的促进河蟹脱壳生长的作用,其作用机制可能与其中氨基酸、小肽、胆固醇等含量丰富有关。     

豆瓣菜不同茎节扦插繁殖试验

以豆瓣菜葡匐茎的上,中、下三段的试材,进行不同茎段的扦插繁殖试验结果表明,中段成活率高,分枝快,适于做扦插材料。     

Spatial and temporal variability of air temperature across urban neighborhoods with varying amounts of tree canopy

Recent studies have emphasized the presence of microclimates in urban settings, but most do not have the high resolution observations necessary to understand the interactions taking place at a neighborhood scale. This study used a network of 10 identical weather stations and high resolution land cover data in Knoxville, Tennessee, to analyze the microclimates of a medium-sized city with a temperate climate. Two stations were installed in each of four urban neighborhoods in locations with varying localized tree cover, and two additional stations were installed in the center of downtown and in a nearby urban nature center. The intra-neighborhood results suggested that there is significant temperature variability within a single neighborhood based on the tree canopy density immediately surrounding a given weather station. However, the inter-neighborhood variability (differences between neighborhoods) was similar in magnitude, which suggests that the overall differences in neighborhood characteristics also have an effect on climate. Land cover at the neighborhood scale (in particular tree canopy percentages at the 500-m radii) had the highest correlation with the minimum daily temperature (Tmin) during the summer season. Maximum daily temperature (Tmax) relied most on the distance of each station from Downtown and the amount of impervious area in the 50 m surrounding each station. Tmax was also most influenced by surrounding land cover during dry conditions (a Dry Moderate air mass). Overall, highly localized impervious land cover percentages and larger-scale forested canopy were important in explaining temperature fluctuation, pointing to the importance of scale in microclimate assessments. Dry air masses enhanced the relationship between land cover and temperature during the day, while moist air masses did the same overnight. These data can be used to better inform planning strategies to build resiliency to extreme heat into urban environments by considering the influence of tree canopy.     

Collection,Analysis, and Utilization of Biogas from Anaerobic Treatment of Crab Processing Waters

Abstract

Two laboratory-scale, upflow anaerobic reactors were constructed to treat blue crab (Callinectes sapidus) cooking wastewater for determination of the volume and composition of the resulting bio-gas. Both systems (A and B) consisted of two 4-liter (L) upflow anaerobic reactors and one 8-L aerobic reactor in series. The first anaerobic reactor of System A contained 240,12.7 mm foam cubes, while the first anaerobic reactor of System B contained 180 foam pieces (25.4 ×25.4 ×12.7 mm). Biogas production rates for System A ranged from 6.6 L gas/L feed to 10.0 L gas/L feed. Mean values for System B ranged from 7.1 L gas/L feed to 11.9 L gas/L feed. Methane and carbon dioxide comprised 68% and 28%, respectively, of the gas from both systems. Hydrogen sulfide averaged 1.5% and 1.4% for systems A and B, respectively. A pilot-scale biogas collection and utilization system was also constructed and successfully demonstrated. Details of the pilot-scale system design are provided in the paper to help processors better understand how the technology might be applied.     

农业院校开展绿色化学教育研究

简要介绍了绿色化学的内涵,阐述了开展绿色化学教育的必要性,提出了开展绿色化学教育的设想与措施。     

榆树叶绿色素的提取与性质研究

研究了从榆树叶中提取天然绿色素的工艺方法以及其榆树叶绿色素的理化性质。结果表明：从榆树叶里提取绿色素时用75%的乙醇（pH值7.80）为提取液效果较好;该色素溶解性和热稳定性良好,在pH值为5～9时较稳定。该色素具有一定的开发利用价值。