结核分枝杆菌分泌蛋白ESAT-6、CFP10基因的克隆、鉴定及其原核表达

以人型结核分枝杆菌 H37RV株基因组 DNA为模板 ,应用 PCR法对 ESAT- 6和 CFP10基因进行扩增 ,产物经纯化后与载体 PMD18- T连接、转化及酶切鉴定 ,亚克隆到原核表达载体 PGEX- 6 P- 1,构建原核重组表达质粒 ,转化入大肠杆菌 BL2 1中 ,以 1mmol/L IPTG诱导 ,进行 SDS- PAGE电泳。结果表明 ,ESAT- 6和 CFP10基因表达的融合蛋白相对分子质量分别为 32 0 0 0和 36 0 0 0 ,与实测相符。重组结核杆菌分泌蛋白 ESAT- 6和 CFP10的成功表达为结核病诊断及重组疫苗的构建打下了基础     

离子对高效液相色谱法测定复方甲硝唑片中甲硝唑和维生素B6含量

建立了同时测定复方甲硝唑片中甲硝唑和维生素B6含量的离子对HPLC方法.采用ODS色谱柱,流动相为磷酸二氢钾和庚烷磺酸钠水溶液(每1L中含磷酸二氢钾13.6 g与庚烷磺酸钠1.0 g)-甲醇-三乙胺(85∶15∶0.4),用磷酸调pH至2.8;检测波长290 nm,以峰面积计算,外标法定量.甲硝唑和维生素B6的线性范围分别为100～300 μg/mL(r=0.999 9)和5～50 μg/mL(r=0.999 9);平均回收率分别为100.2%(RSD=1.0%,n=6),99.8%(RSD=1.2%,n=6).该方法测定结果准确,操作简便,回收率高.     

低温对2种紫草科植物△6 脂肪酸脱氢酶基因表达的影响

以紫草科植物新疆软紫草(Arnebia euchroma)和假狼紫草(Nonea capsica(willd.)G.Don)为材料,翻译延伸因子EF1a为内参基因,利用半定量逆转录聚合酶链式反应(semi-RT-PCR)技术,对2种紫草植物中γ-亚麻酸合成关键酶基因△6-脂肪酸脱氢酶基因的表达进行研究。结果表明,△6-脂肪酸脱氢酶基因在紫草植物的叶、茎、根中均表达,在低温诱导不同时间后紫草植物叶片中△6-脂肪酸脱氢酶基因的表达有所增强。气相色谱分析显示,其产物γ-亚麻酸在低温下积累,推测紫草科植物叶片中△6-脂肪酸脱氢酶基因表达量增加可能与适应低温胁迫的逆境有关。     

Caterpillar G3412型燃气发电机排障实践

介绍了Caterpillar G3412型燃气发电机的基本工作原理,分析了涩宁兰输气管道涩北首站该型燃气发电机拉缸的故障情况。通过更换2号缸的缸桶、活塞、气环和油环,以及高压线圈、O形圈、火花塞、水湿传感器等附件,使发电机故障得以排除,同时提出了发电机组日常运行的维护方法。     

基于3G通信与GPS技术的农机视频监控系统研究

针对现代化大农机发展的需求,研究了如何将3G通信技术与GPS定位技术相结合,建立农机视频监控系统,用于现代化大农机管理。采用WebGIS技术为基础,以B/S结构进行开发建设,为农机综合管理监控提供了新思路、新手段。     

GLIS6下用EXCEL数据实现读者信息批量快速导入的方法

为新生读者办理借阅证是高校图书馆流通管理的一个基础环节,如何短时间内为学生办好借阅证,是摆在各家图书馆面前一项急需解决的问题。通过在实践中摸索,找到了一种利用Excel数据,实现读者信息批量快速导入SQL数据库的方法,从而极大地提高了办理借阅证的效率。     

山丹马6-磷酸葡萄糖脱氢酶及葡萄糖磷酸异构酶的多态性研究

用淀粉凝胶电泳及琼脂覆盖技术对山丹马的血液红细胞6磷酸葡萄糖脱氢酶和葡萄糖磷酸异构酶的电泳变异进行了测定。在6磷酸葡萄糖脱氢酶座位发现了3种表现型,即FF,FD和FS,其频率分别为0.958,0.021和0.021。在葡萄糖磷酸异构酶座位发现了两种表现型,即II和FI,其频率分别为0.771和0.229。等位基因频率直接通过表型计算出:PGDF为0.979,PGDD为0.0104,PGDS为0.0104;GPIF为0.1146,GPII为0.8854。亲子关系排除概率在6PGD和GPI座位点分别为0.1009和0.0912。两座位的个体识别概率分别为0.081和0.353。     

Effect of pyrethroids on carbohydrate metabolic pathways in common carp,Cyprinus carpio

The activity levels of succinate dehydrogenase (SDH) and glucose‐6‐phosphate dehydrogenase (G6PD) were assessed in various tissues of Cyprinus carpio var communis which had been exposed to lethal concentrations of group‐II pyrethroids (deltamethrin, cypermethrin, fenvalerate and fluvalinate) for a period of 72 h. The results indicated a steady decrease in SDH activity with a concomitant increase in G6PD activity. The decreased SDH activity indicated inhibition of SDH at mitochondrial level and the increased G6PD activity an enhancement of an alternative pathway of carbohydrate metabolism, viz the hexose monophosphate shunt (HMP) or pentose phosphate pathway as a biochemical adaptation to overcome the toxic stress. © 2001 Society of Chemical Industry     

Effects of N-methyl-D, L-aspartate on secretion of growth hormone-releasing hormone from the boar hypothalamic-preoptic area and median eminence in vitro

N-methyl-D, L-aspartate (NMA) elicited secretion of growth hormone (GH)-releasing hormone from both the hypothalamic-preoptic area and the median eminence that were collected from boars. We suggest that the previously described increase in GH secretion that follows peripheral treatment of swine with NMA is attributable, at least in part, to NMA-stimulated secretion of GH-releasing hormone from the central nervous system.     

Flowering-pattern and yield components at inflorescence nodes of snap bean as affected by irrigation and plant density

This paper reports the results of a 2 × 2 factorial experiment on bush snap beans ‘Oregon 1604’. The treatments were 2 contrasted irrigation regimes and 2 contrasted plant densities, and were applied in 1978 and repeated in 1979. Data were collected on the number of flowers and pods, and pod size, at each node of the terminal inflorescence (6-T) of the main stem, and at each node of the oldest inflorescence (2-A) at Node 2. High and low plant densities were 45 and 18 plants m^?2 in 1978 and 54 and 33 plants m^?2 in 1979. High temperatures, frequently above 32°C, prevailed during bloom and pod development in 1978, but for the most part occurred only during the week prior to bloom in 1979. Inflorescences 6-T and 2-A usually formed 4 and 3 RN's, respectively, in 1978 and 3 and 2 RN's in 1979. The flowers at the proximal nodes of each inflorescence all opened within a few days of one another (duration of flowering at proximal nodes between 3 and 5 days); the flowering-periods of adjacent nodes overlapped, and the flowering period increased acropetally within the inflorescence (duration of flowering at distal nodes between 7 and 13 days). In general, number of flowers, pods formed, pods harvested and percent set decreased acropetally within each inflorescence. The rate of acropetal decline was lessened by high irrigation or low plant density. In both years, high irrigation increased the percent set of all RN's of the 2-A inflorescences, but few other consistent effects between years were observed. The 2 most proximal RN's together produced 93% or more of the yield of each inflorescence. High irrigation significantly increased the total number of pods harvested from these RN's of inflorescences 6-T and 2-A, and low density had a similar effect on 2-A.