白菜型油菜DFR基因的克隆与序列分析

By using RACE (rapid amplification ofcDNA ends) based homologous cloning strategy, we have successfully isolated the genomic and full-length cDNA sequences of a gene encoding typical DFR (dihydroflavonol-4-reductase) from black-seeded Brassica campestris L. var. oleifera DC.. The gene, designated BcDFR here, is 1 722bp in length and harbors 5 introns with typical splice sites of plant DFR genes. BcDFR cDNA is 1311bp in length with a 1 158bp ORF as well as a 25bp 5‘ UTR and a 128bp 3‘ UTR. The encoded BcDFR protein is 385 aa with a calculated Mw of 42.85kD and a pI value of 5.55. The nucleotide and amino acid sequences of this gene share extensive homologies to plant DFR genes of wide origins especially high similarities to Cruciferous DFR genes. Sequence analyses such as phylogenetic analysis, conserved domain search and substrate specificity region detection all indicated that BcDFR gene is a quite potentially biofunctional gene. Its cloning enables us to further dissect the possible relatedness between DFR gene and Brassica seed coat color traits and to create transgenic novel yellow-seeded rapeseed germplasm through antisense- or RNAi-suppression of DFR gene expression in black-seeded elite cultivars.     

青花菜组培中过氧化物酶及吼哚乙酸氧化酶与形态发生的关系

青花菜在脱分化及分化过程中其过氧化物酶活性升高;脱分化培养的第12天该酶活性的大幅度增加与愈伤组织的明显发生相对应;分化培养的第21天该酶活性的大幅度增加与真正的芽发生相关.脱分化时过氧化物酶同工酶的c带、e带发生在愈伤组织产生以前,可能是脱分化的原团;分化时c带在芽点出现以前的重新出现可能与分化的启动有关.在脱分化过程中吲哚乙酸氧化酶活性略有升高;在分化过程中该酶活性基本保持稳定.     

尿素配施氯化铵对菜心产量及其品质的影响

利用盆栽试验，研究尿素配施不同比例的氯化铵对菜心硝酸盐积累的影响，结果表明：随着尿素配施氯化铵比例的增加，菜心氯含量增加，硝酸盐含量下降，而全N含量、叶绿素含量和收获前的产量变化不大。     

A Spot Price Model with Var Cost

This paper has applied the var cost to the theory of spot price.An augmented Lagrange method is employed to develop the exact model of spot price of active power and reactive power,and the physical means of Lagrange multipliers are explained.The numerical examples are given to demonstrate the validity of the proposed model.     

油初始坐果机制研究

以成年油木奈树为材料,对其初始坐果进行了研究。结果表明,盛花后2周内,油木奈出现大量的落花落果;幼果(子房)低水平的GA1 3与油木奈的落花落果高峰关系密切,而内源细胞分裂素ZRs和[diH]ZRs以及ABA与幼果(子房)的生长呈显著的正相关;初始坐果期,油木奈的雌配子体发育缓慢,且有相当数量的胚囊中途退化、败育,无法正常受精,从而导致其大量的落花落果。文中就油木奈的初始坐果机制进行了讨论。     

辽西地区引种野杏数量性状研究

以辽西地区26个野杏无性系为试材,采用变异性分析、方差分析以及主成分分析等方法,研究了其25个数量性状的变异特点,以期为野杏无性系的遗传多样性研究和良种选育提供重要参考依据。结果表明:19个数量性状呈正态分布;25个野杏无性系数量性状变异系数均值23%,其中22个数量性状的变异系数在10%以上,小枝长度变异系数最大,达64%,单果质量、单核质量、单仁质量的变异系数分别为24%、21%和18%,表明野杏无性系数量性状变异程度丰富,选择潜力较大。野杏无性系果实性状重复力普遍偏高,核仁主要性状重复力次之,树体主要数量性状重复力较二者偏低,均达到极显著水平;果核仁性状的重复力除单果质量、单核质量和仁厚分别为0.634、0.756和0.624外,其它指标皆在0.909以上,其中仁长重复力最高,达0.973。主成分分析结果表明,前6个主成分累积贡献率达83.50%,能够反映野杏无性系数量性状的大部分信息;其中第1主成分主要反映野杏果核仁等经济性状。     

超声波-微波协同法提取分蘖葱头有机硫化合物的工艺优化及其组分分析

以分蘖葱头为试材,采用超声波-微波协同法提取有机硫化合物,进行分蘖葱头有机硫化合物工艺优化试验,并利用气相色谱-质谱联用仪对提取物组分进行分离鉴定,以期为进一步研究和开发分蘖葱头功能组分提供参考依据。结果表明:最佳提取工艺为微波温度55℃、料液比1∶8 g·mL^-1、协同时间6 min、搅拌转速300 r·min^-1、超声波功率500 W,该条件下有机硫化合物得率为0.2771%±0.0023%。该条件得到15种有机硫化合物,含量较高的是噻吩类39.0678%±0.0043%,其次是三硫化合物14.1570%±0.0071%和硫醚类7.3872%±0.0052%,其中含量相对较高的有机硫化合物为2,4-二甲基噻吩、3,5-二乙基-1,2,4-三硫杂环戊烷、二烯丙基二硫醚。     

抗生素对黄叶日本桃叶珊瑚试管苗细菌污染抑制效果初探

采用医用抗生素氨苄西林钠、硫酸链霉素、头孢拉定及其组合抑制黄叶日本桃叶珊瑚试管苗细菌污染试验结果表明：单独使用1种抗生素时,抗菌及试管苗生长、生根情况不如组合使用效果好,使用抗生素氨苄西林钠比另外2种抗生素效果好;组合使用抗生素时,培养基附加氨苄西林钠与硫酸链霉素各50 mg/L,对污染菌的抑菌率为100%,同时在增殖系数、苗高、生长势方面都表现最好,并对黄叶日本桃叶珊瑚试管苗的生根有明显的促进作用,氨苄西林钠与头孢拉定抗菌组合效果次之,效果好于硫酸链霉素与头孢拉定组合。     

福建省水稻霜霉病诊断和病原鉴定

水稻霜霉病诊断特征为植株矮缩,叶片黄化、产生黄褐色条斑.利用显微镜检查染色病组织内的病原菌藏卵器和卵孢子,通过观察病原菌的培养性状、形态特征和致病性测定,该病原菌被鉴定为大孢指疫霉水稻变种.     

Mineral Composition and Soil-Plant Relationships for Common Guava (Psidium guajava L.) and Yellow Strawberry Guava (Psidium cattleianum var. lucidum) Tree Parts and Fruits

The mineral compositions of the fruit and tree parts of common guava, Psidium guajava L., and strawberry guava, Psidium cattleianum var. lucidum, were determined. The study occurred during three seasons at six locations in Hawaii to assess guava as feed for livestock. Guava bark contained the greatest concentrations of calcium (Ca) and ash; leaves the greatest concentrations of magnesium (Mg), sulfur (S), sodium (Na), boron (B), and manganese (Mn); and the shoots had the greatest concentrations of nitrogen (N), phosphorus (P), and potassium (K). The leaves and the shoots had the greatest concentrations of copper (Cu) and iron (Fe). Between guava and waiwi, guava had greater concentrations of most minerals except for Na in all plant parts, and Mg and ash in the leaves. Guava leaves and shoots meet the macromineral requirements for various phases of sheep, goat, and beef cattle life cycles with the exception of P and Na. Guava shoots do not meet Mn requirements for lactating cows.