广西农垦良圻原种猪场应用新工艺、新技术效果显著

广西良圻原种猪场是第一批国家生猪核心育种场、国家生猪产业技术体系永新综合试验站，也是广西第一批重点种猪场和健康种猪场。最近该场肖有恩总经理在广西健康养猪经验交流会上重点介绍良圻原种猪场生产经营及相关新工艺、新技术运用情况，工艺先进成绩显著，经验很好，值得借鉴和推广，主要介绍如下几个方面。     

鹌鹑曲霉菌与大肠杆菌混合感染的诊治

鹌鹑曲霉菌病是曲霉菌引起的疾病，大多由于饲料、垫料发霉污染而引起。大肠杆菌病是由大肠埃希氏菌的某些血清型所引起的一类多种动物疾病的总称。2008年7月，我市某特禽养殖场周某饲养的19日龄的鹌鹑发生了曲霉菌与大肠杆菌混合感染，现将其发病和诊治情况报告如下。     

秸秆不烧变现钞

麦收刚过，在一些地方因焚烧小麦秸秆而狼烟四起的时候．记者在地处鲁西的聊城市看到了完全不同的一幕：在高唐县泉林纸业公司原料场，数十个大草堆间隔有序．一辆辆满载小麦秸秆的运输车排成了壮观的金色长龙。正在等待过磅称重的聊城市经济开发区北城街道办事处农民王德强告诉记者．以往许多麦秸都烧了，现在．麦秸有用途了．农民也能增加点收入，“俺这一车能净赚1500块左右。”     

创建稻麦亩产吨粮市(县)的内容与保障措施

为落实江苏省关于开展亩产吨粮市(县)创建工作意见,立足扬中市稻麦生产实际情况,以实施农业现代化工程为契机,以大面积水稻平均产量9 750 kg/hm2、小麦产量5 250 kg/hm2为目标,明确了4项重点建设内容,提出了4条创建保障措施。     

Screening and Analysis of Proteins Interacting with TaPDK from Physiological Male Sterility Induced by CHA in Wheat

To further research the regulatory network of pyruvate dehydrogenase kinase (designated as TaPDK) in physiological male-sterility (PHYMS) of wheat induced by chemical hybridizing agent (CHA) SQ-1, an anther cDNA library was constructed, and the proteins interacting with TaPDK were screened via yeast two-hybrid technique. Subsequently, a few candidate proteins in nucleotide expression levels were detected by real-time quantitative PCR. Yeast-two hybrid screening was performed by mating yeast strain Y2HGold containing BD-TaPDK bait plasmid with yeast strain Y187 including anther cDNA library plasmid. Diploid yeast cells were plated on synthetic dropout nutrient medium (SD/-Ade/-His/-Leu/-Trp) (QDO), and further were incubated on QDO medium containing AbA and X-α-Gal. The interactions between TaPDK and the proteins obtained from positive colonies were further confirmed by co-transformation validation. After plasmids DNA were extracted from blue colonies and sequenced, the sequences results were analyzed by bioinformatic methods. Finally, 24 colonies were obtained, including eight genes, namely non-specific lipid-transfer protein precursor (TanLTP), polyubiquitin (TaPUbi), glyceraldehyde-3-phosphate dehydrogenase, proliferating cell nuclear antigen (TaPCNA), CBS domain containing protein (TaCBS), actin, guanine nucleotide-binding protein beta subunit, chalcone synthase, and three new genes with unknown function. The results of quantitative RT-PCR showed that the expression levels of TanLTP, TaPUbi, and TaPCNA were obviously up-regulated in PHYMS anther, and TaCBS expression was only increased at the tricellular stage in PHYMS anther compared with in fertile lines. Whereas, the expression of TaPDK was obviously down-regulated in PHYMS lines. Collectively, these datas indicated that the majority of candidate proteins might be related to pollen abortion in PHYMS lines, which further suggested that TaPDK plays multiple roles in pollen development, besides participating in regulating pyruvate dehydrogenase complex activity.     

他山之石 可以攻玉

在青少年的网球之路上,充满了希望和梦想,但辛苦与汗水也同时存在于他们的身旁,对于从事网球运动的女孩子而言,她们则需要付出更多的努力。本文通过从80年代至今的WTA球员的技战术特点来为青少年女子网球的发展思路作出分析,希望通过对球员身体条件、比赛风格等方面的深入发掘,为众多家长和教练提供新的理念。     

絮凝剂PAC处理水体中含氮化合物的效果

选择絮凝剂聚合氯化铝(PAC)为研究对象,研究了通过絮凝及降解作用去除水环境中的亚硝基氮、氨氮、总凯氏氮的规律和效果,并确定了絮凝沉淀的最佳工艺参数。研究结果表明,当PAC的投加量为40mg/L时,硝基氮的去除率达到30.71%,亚硝基氮的去除率为77.27%,氨氮的去除率为37.5%,总氮(TKN)的去除率为40%。     

猪附细胞体病并发猪伪狂犬病病例的诊治

猪附红细胞体病是由立克次氏体目中的附红细胞体引起猪的一种以急性黄疸性贫血和发热为特征的传染病。又称为黄疸性贫血、类边虫病、赤兽体病或红皮病,其临床特征是呈现急性黄疸性贫血和全身皮肤发红,故又称红皮病。该病2002年在德宏州有疫情报告。伪狂犬是由伪狂犬病毒引起的猪和多种动物共患的一种以发热,在临床上是以神经症状为主要特征的急性传染病。德宏州是该病散发区,早在1989年德宏动物疫病调查报告中就有记载。     

第一批国联＂南三1号＂种虾找到了＂婆家＂!

“浅色袋装的是雌性种虾，深色袋装的是雄性种虾。” “捕捞前已经泼洒了应激灵，水位也降到20—25cm．”