优质油菜在我国南方农业中的战略地位及其发展方略

我国是一个油料消费和进口大国,油菜是我国面积最大和发展最快的油料作物。我国油菜90％分布在南方,南方尚有大量的冬闲田可以利用,发展油菜可充分利用南方的自然资源、缓解南方人地矛盾,有利于增油、增粮、增饲、增肥,有利于南方种植业结构的调整和相关产业的发展,有利于农民增收增效。针对我国目前油菜产业存在的品质差、成本高和综合效益低等主要问题,提出了我国油菜产业发展应采取科技兴油、区域化、产业化和品牌营销战略。     

苯丙氨酸解氨酶活性与玉米对纹枯病抗性的关系

苯丙氨酸解氨酶活性变化在玉米抗、感品种和不同生育期与叶鞘位之间存在差异。在玉米(川单10号)的不同生育期和叶鞘位中,随生育期的发展和叶鞘位的下降,苯丙氨酸解氨酶活性降低。在受纹枯病菌侵染后,抗病品种(R15)的苯丙氨酸解氨酶活性增加的速度和程度明显高于感病品种(K09)。这一结果表明,玉米对纹枯病的阶段抗性变化以及品种抗性与苯丙氨酸解氨酶活性变化相关。     

Detection of papillomavirus-DNA in mesenchymal tumour cells and not in the hyperplastic epithelium of feline sarcoids

Abstract We examined 12 formalin-fixed paraffin-embedded feline skin tumours which had the histopathological features of fibropapillomas for the presence of papillomavirus (PV) DNA using touchdown polymerase chain recation (PCR), DNA sequencing and nonradioactive in situ hybridization. Nine of the tumours contained a 102-bp PCR product demonstrated using consensus PV primers that amplify a portion of the L1 gene. The nucleotide sequences are closely related, but not identical to that of ovine PV type 2, rabbit oral PV and reindeer PV. The deduced amino acid sequences had strong homologies with the major capsid protein L1 of deer PV, bovine papillomavirus (BPV) 1 and BPV 2, and European elk PV. Although PV antigens were not detected in any of the tumours by immunohistochemistry, PV DNA was demonstrated in individual mesenchymal cells or cell nests of 4/12 tumours by in situ hybridization. A nonproductive infection of mesenchymal fibroblast-like tumour cells with a papillomavirus would explain the lack of PV antigen expression and the absence of PV DNA in the hyperplastic epithelium. Because these tumours and their pathogenesis are similar to equine sarcoids, we suggest that they should be reclassified as 'feline sarcoids' instead of fibropapillomas.     

深圳碧海湾高尔夫球场草坪春季杂草及防除策略研究

对深圳碧海湾高尔夫球场草坪进行调查,发现春季草坪杂草有95种,分属26科80属。阔叶杂草有75种,占杂草总数的78.95%;半日子叶杂草20种,占杂草总数的21.05%。对球道和长草区或边坡的杂草发生特点分别进行分析。针对杂草的发生来源和特点,提出了草坪杂草的防除策略。     

高寒地区一年生人工草地地上生物量动态及光能转化效率

对燕麦单播草地、燕麦+箭舌豌豆混播草地牧草组成及地上生物量动态,光能转化效率进行了观测和分析。试验结果表明,混播草地的两组分在生长发育节律和牧草产量积累过程方面均存在着较大的差异,箭舌豌豆与燕麦干物质之比随牧草生长发育的进展而逐渐增大。2种草地的干草产量在牧草生长期间差异不显著。8月中旬,单、混播草地干物质分别可达10.19、11.74t/hm2,地上净初级生产力分别为18874.69、21204.79kJ/(m2·a),光能转化率分别达0.3502%、0.3922%。     

江河源区退化草地鼠害的治理研究

针对江河源区草场退化、高原鼠兔危害严重的状况,采用了翻耕、耙耱、撒播、轻耙、镇压等一系列治理措施.结果表明,这种以生态工程为主的治理措施,不仅使草场植被在短期内得到恢复,而且害鼠种群数量也得到了有效控制;同时,为防止鼠兔数量回升、减少扩散、播种牧草幼苗免遭啃食和挖掘破坏,在不同处理区又采取了以药物防治为辅的控制措施.在现场药效试验和大面积灭鼠中,0.075%敌鼠钠盐、0.1%C型肉毒杀鼠素和0.1%D型肉毒杀鼠素毒饵对鼠兔均具有良好灭效,且不污染环境,无二次中毒,对保护天敌、发挥生物控制危害具有一定意义.因此,在退化草场鼠害治理中,综合分析各组分之间的相互关系,有针对性地进行治理,才能收到良好效果,从而达到综合治理的目的.     

Tank-mix adjuvants and pesticide residues: some regulatory and quantitative aspects

Although it is well known that judicious use of adjuvants can increase the performance of foliage-applied sprays of many agrochemicals, little information is available in the public domain about their ultimate effects on pesticide residues in treated crops. In the present work, the influence of Agral (polyoxyethylene nonylphenols), Toil (methyl esters of rapeseed fatty acids) and Bond (styrene-butadiene copolymers) on surface and crop residues of diclofop-methyl/diclofop and propiconazole in wheat and field beans was investigated using a model system simulating field practice. Pesticides were applied as commercial formulations, diclofop-methyl 378 g litre(-1) EC (Hoegrass) and propiconazole 250 g litre(-1) EC (Tilt), at their maximum approved rates, 1135 g AI ha(-1) and 125 g AI ha(-1), respectively, both in the presence or absence of the maximum rate recommended for each candidate adjuvant. No detectable residues of diclofop-methyl or propiconazole were found in wheat 35 days after any of the four applications. However, residues of diclofop were present in this crop, and those from applications containing Agral (0.07 mg kg(-1) fresh weight (FW)) or Bond (0.08 mg kg(-1) FW) were significantly lower than those with no adjuvant (0.14 mg kg(-1) FW) or Toil (0.16 mg kg(-1) FW). Unlike wheat, residues of both diclofop and propiconazole were detected in field beans after harvest. Significantly higher residues of the former were recorded from the applications with Agral or Bond (ca 0.32 mg kg(-1) FW) than with those with no adjuvant or Toil (ca 0.15mg kg(-1) FW). All the propiconazole applications containing adjuvants showed a similar significant increase in residues (0.10-0.16 mg AI kg(-1) FW) over the no-adjuvant treatment (0.05 mg kg(-1) FW) in this crop. There appeared to be little agreement between the apparent amounts of uptake, as indicated by the rates of decline of surface residues up to 5 days after application, and final residues in either target species. On wheat, surface residues of diclofop-methyl decreased from initially ca 20 to as little as 0.02 mg kg(-1) FW using adjuvants; the corresponding values for propiconazole were ca 2 to ca 0.03 mg kg(-1) FW. Recoveries of diclofop-methyl from the surfaces of field beans were much higher than those from wheat, declining from ca 30 to only ca 6 mg kg(-1) FW during the course of 5 days; the corresponding residues for propiconazole were ca 2 mg to 0.15 mg kg(-1) FW. These findings are discussed in relation to uptake results obtained with radiolabelled pesticides and adjuvants in the laboratory, and to the mandatory requirements for pesticide residue data for the authorised use of adjuvants in the UK.     

试析转型期文学的发展

二十世纪90年代,世界格局发生了根木的变化,人类试图在“全球一体化”的语境中寻求共同的利益。经济领域计划经济向市场经济的转型势必会引起上层建筑中文化、意识的转型。转型期的中国当代文学呈现出现代主义”与后现代主义”文化语境互相渗透,知识分子人文意识的变化与失落,文学创作的精神属性淡化与商品生产强化的特点。     

Double in situ hybridization for simultaneous detection and differentiation of porcine circovirus 1 and 2 in pigs with postweaning multisystemic wasting syndrome

Double in situ hybridization using a digoxigenin-labelled porcine circovirus 1 (PCV1) and biotinylated PCV2 probe, was developed for the simultaneous detection and differentiation of PCV1 and PCV2 in formalin-fixed, paraffin-embedded tissues from pigs with postweaning multisystemic wasting syndrome. The combination of an alkaline phosphatase conjugated antidigoxigenin system with alkaline phosphatase conjugated streptavidin-biotin system allowed identification of PCV1 and/or PCV2. No evidence of cross-reaction was observed. Positive cells exhibited a red or dark brown reaction product for PCV1 and PCV2, respectively. Both PCV DNAs were observed mainly in the cytoplasm but occasionally in the nucleus. Co-localization of hybridization signal for both PCV1 and PCV2 was present in macrophages and multinucleated giant cells of the lymph node and spleen. This double-labelling technique for the differentiation between PCV1 and PCV2 is suitable for pathogenesis studies and diagnostic applications.     

加速结构调整是大兴安岭可持续发展的必然

文章指出以“天保工程”为契机 ,以林业的持续发展为目的 ,调整大兴安岭林业生产结构 ,发挥其多功能互助的整体效益