Expression of PDGFR-β and Kit in canine anal sac apocrine gland adenocarcinoma using tissue immunohistochemistry

Canine anal sac apocrine gland adenocarcinoma (ASAGAC) is an uncommon but highly invasive and metastatic malignancy. Toceranib phosphate (Palladia) is a receptor tyrosine kinase (RTK) inhibitor that targets several members of the split kinase RTK family. These membrane receptors are important for cell cycling, apoptosis and angiogenesis, all of which can contribute to carcinogenesis. The objective of this study was to evaluate archived, paraffin-embedded canine ASAGAC and normal canine anal sacs for immunohistochemical detection of Kit and platelet-derived growth factor receptor beta (PDGFR-β). Two of 77 neoplasms (2.6%) expressed Kit. Fifteen of the neoplasms (19.5%) were positive for PDGFR-β expression. None of the normal canine anal sac epithelium expressed Kit or PDGFR-β. Because of these results, further investigation should be considered to determine the role of RTKs in the clinical course and treatment of canine ASAGAC.     

h指数及其衍生指标评估兽用疫苗领域单篇专利的适用性探索

随着禽流感、非洲猪瘟等疾病的出现,兽用疫苗的开发研制成为了动物防疫体系建设的重要一环,同时兽用疫苗领域国际竞争日益激烈,直接影响到我国相关企业的生存和发展,因此国家必须加强对兽用疫苗领域专利质量和专利保护的重视.本文以计量学中的h指数及其衍生指标为对象,研究其与传统专利价值评估指标的关系,通过相关性分析来筛选评估兽用疫苗领域高价值专利的指标.结果表明:h指数、g指数和hg指数这三个指标,与传统专利价值评估指标相关性强,且计算简便,相对于传统指标,能够快速准确地对兽用疫苗领域专利价值进行预估.     

动物性食品中抗菌药物残留检测方法研究进展

抗菌类药物在预防、治疗动物疾病中发挥了重要的作用,但不规范用药、超剂量用药甚至滥用,使动物性食品中抗菌类药物残留问题日益突出,引起人们的广泛关注。对动物性食品中抗菌类药物残留的检测是判定其安全性的重要内容,根据检测的目的和需要,可将检测方法分为现场检测和实验室检测。胶体金免疫层析法是目前使用最广的现场检测方法;实验室检测方法众多,又可分为抗菌类药物残留的定性检测法和定量检测法,前者主要包括2,3,5-三苯基氯化四氮唑法（TTC法）、纸片法（PD法）、活性检测法等,后者主要包括高效液相色谱法、液相色谱-质谱联用法、超高效液相色谱-质谱联用法、毛细管电泳法、生物传感器法和免疫分析法等,可满足不同检测目的和实验条件的需要。作者对肉、蛋、奶中抗菌类药物残留的现场检测和实验室检测技术进行了总结,为更进一步研究快速、敏感、高通量、成本低廉的食品中抗菌类药物残留检测方法奠定理论基础。     

动物源性食品中尼卡巴嗪残留量的测定

动物产品中的尼卡巴嗪药物残留已被我国及美国、日本、欧盟等国家和地区列为重点检测内容,其最大残留量为0．01mg／kg-0．2mg／kg不等。为探讨合适的检测方法,本文对国内外相关尼卡巴嗪的分析检测方法的优缺点做了分析和比较,并重点介绍了动物源性食品中尼卡巴嗪残留量检测的高效液相色谱法（HPLC）和高效液相色谱-质谱联用（HPLC-MS）法。运用这两种方法进行检测,其最低检测限可达到10μg／kg甚至0．5μg／kg,基本上符合现有相关标准的限量要求。     

Estimating symbiotic N2 fixation in Robinia pseudoacacia

Estimating symbiotic di‐nitrogen (N₂) fixation is challenging, especially when working with woody N₂ fixers in field trials. Fortunately, isotope methods based on ¹⁵N natural abundance or on ¹⁵N artificial enrichment (dilution method) make it possible to estimate the proportion of nitrogen derived from the atmosphere (Ndfa) in N₂‐fixing species. These methods have been extensively used in the field for herbaceous species, much less for tree species such as alder and acacia, and rarely for black locust (Robinia pseudoacacia). The objectives of this study were to characterize the fixation potential of black locust in a plantation by using the two ¹⁵N isotope methods in the field, and to document values of isotope fractionation occurring during N₂ fixation (the B value). B values were estimated both by growing trees on an N‐free medium in controlled conditions (B_lab) and by making Ndfa calculated with the natural abundance method converge with Ndfa calculated with the ¹⁵N dilution method in the field (B_field). The two methods gave consistent estimates of the B value. B values ranging between –1.4 and –3.2‰ were found, varying with the age of the plant material. Up to 76% of the N in the black locust trees came from the atmosphere, representing more than 45 kg N ha^?1 over five years and confirming that black locust may be well adapted to N‐poor soils.     

玉米种质系谱——武105和原武02类群的划分与论述

通过1957-2004年近50年对全国玉米自交系衍生系的种质变化材料进行广泛的收集、积累与整理研究,笔者将武105和原武02类群划分为玉米系谱十大种群之第二类。武105为优良自交系品种,在顶交种、双交种、三交种、早熟单交种方面得到了广泛利用,并进一步育成了陕单一号、黄白一号、陕单七号等,后又衍生了武109、天4等优良改良系,组配成了陕单号、户单号主要推广骨干杂交种; 山东农业科学院通过辐射处理育成原武02后,进而衍生改良了原齐号、鲁原133、鲁原92等后续系,形成了新的衍生分枝,也得到了广泛的利用;后续又衍生成新的改良系,在江苏省苏玉系列品种中占有很大比重;另在吉林通化、福建一脉相承,得到应用。武105种质西种东移,南延北扩,丰富了我国玉米种质基因库。     

浅析动物性食品中性激素残留与检测

随着畜牧和养殖业的飞速发展,各种兽药、饲料添加剂的使用范围逐渐扩大,然而在取得经济效益的同时也使动物性食品中兽药和违禁药物残留对人体健康产生影响,其中激素残留对消费者健康的影响更值得关注。作者讨论了动物性食品中性激素残留的危害,探讨了利用现代分析技术检测动物性食品中性激素残留的方法,以期为生产中检测激素残留提供参考。     

农药在动物体内残留代谢的研究

农药在动物体内的代谢残留,不仅直接影响机体健康,而且影响养殖业的发展,成为我国动物源性食品出口的主要制约因素。综述了农药在动物源性食品中残留的原因、现状、危害,并提出了农药残留的防控对策。     

动物源性食品中糖皮质激素残留检测方法的研究进展

糖皮质激素常用于治疗家畜的炎症反应、免疫性疾病、牛的酮病等,并且能提高饲料的转化率,促进畜禽的生长,因而广泛用于畜牧业中。然而,动物生长过程中过量使用糖皮质激素会导致其在动物源性食品中残留,给人体健康带来极大的危害。世界各国对动物源性食品中糖皮质激素残留实施愈来愈严格的监控,对各种检测方法也提出了越来越高的要求。本文介绍了糖皮质激素在畜牧业中的应用、糖皮质激素残留的危害,并对其残留检测方法进行综述。