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41.
用法国*本地二元杂交鸭为研究对象,分析其宰前血浆GOT、GPT、Akp、r-GT、Amy活性与7个产肉性状的表型相关,结果表明GOT活性与屠宰率,胸肌率,腿肌率,观众 率,GPT活性与胸肌率,瘦肉率、r-GT活性与胸肌率,腹脂率均呈显著正相关; 相似文献
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鸡源致病性大肠埃希菌中氨基糖苷类抗生素耐药基因的检测 总被引:1,自引:0,他引:1
为了解鸡源致病性大肠埃希菌对氨基糖苷类抗生素的耐药性变化和钝化酶耐药基因的携带情况及耐药基因与耐药性的相关性,从陕西、河南、河北、山西、宁夏和甘肃6省(区)的部分规模化养鸡场的病、死鸡中分离鉴定320株致病性大肠埃希菌。采用K-B药敏纸片法检测分离菌对6种氨基糖苷类药物的敏感性,PCR方法检测6种氨基糖苷类钝化酶耐药基因,用DNA Star软件对获得的耐药基因序列与GenBank中的相关序列进行比对。结果显示,鸡源致病性大肠埃希菌分离株对庆大霉素、链霉素、妥布霉素、卡那霉素、新霉素和阿米卡星的耐药率分别为53.4%、49.3%、37.5%、34.7%、22.8%和5.3%,对妥布霉素和卡那霉素耐药率呈上升趋势,而对庆大霉素的耐药率虽呈下降趋势,但仍维持在40%以上。3重以上耐药菌株占80%(256/320)。氨基糖苷类钝化酶基因aac(3)-Ⅱ、aph(3′)-Ⅰ和aac(6′)-Ⅰ的检出率分别为50.9%、25.9%和3.1%,未检测到aac(3)-Ⅳ、ant(3′′)-Ⅰ和aph(3′)-Ⅱ基因。研究表明,分离的鸡源致病性大肠埃希菌对氨基糖苷类抗生素的耐药性普遍存在,以多重耐药为主,且对妥布霉素和卡那霉素的耐药性不断上升。耐药基因aac(3)-Ⅱ和aph(3′)-Ⅰ的检出率与其耐药性呈正相关。 相似文献
44.
Yea Hwang MOON Ji Un OK Shin Ja LEE Jong Kyu HA Sung Sill LEE 《Animal Science Journal》2010,81(6):642-647
A comparative study among Korean native cow (Hanwoo), Holstein dairy cow, Korean native goat and crossbred sheep on the population and marker concentration of ruminal microbes, the activities of carboxymethylcellulase (CMCase), xylanase and amylase, and in situ dry matter (DM) degradability were conducted. Twelve ruminally cannulated animals, three of each species, were used. Animals were fed the same diet containing 40% formula feed and 60% rice straw at the level of 2.5% of body weight. Total viable microbial populations in the rumen fluid were significantly (P < 0.01) greater for bacteria and fungi in goat than those of Holstein. The protozoan population among ruminant species was the reverse from that of bacteria. The concentrations of 2,6‐diaminopimelic acid and chitin as markers for bacteria and fungi in the rumen fluid, respectively, were highest in goat, which is in accordance with the above population data. The concentration of aminoethylphosphonic acid as marker of protozoa was highest in Hanwoo and lowest in sheep (P < 0.01). Goat had the highest (P < 0.01) activities of all the enzymes investigated among ruminants. In situ effective degradation of the DM of rice straw was approximately 19% higher in the rumen of goat compared with other animals. 相似文献
45.
乳酸菌和纤维素酶对早籼稻秸秆青贮饲料品质的试验 总被引:1,自引:0,他引:1
为了开发利用南方丰富的稻草秸秆资源,满足奶水牛生产的需要,在稻草中添加复合乳酸菌与复合纤维素酶的方法青贮,采用不同的复合乳酸菌与复合纤维素酶添加水平,试验设对照组、试验1组、试验2组和试验3组进行处理,常温条件下青贮35d后开封进行青贮饲料品质评定。结果表明:试验各组青贮饲料中DM和ADL含量无显著变化(P0.05),而其它指标均有显著变化(P0.05)。CP含量随复合乳酸菌制剂和酶制剂添加量的增加而增大,其中试验Ⅲ组比对照组高8.41%;NDF和ADF含量随乳酸菌制剂和纤维素酶制剂添加量的增加而下降,试验Ⅲ较对照组低10.92%和0.48%。试验结果表明乳酸菌制剂和纤维素酶制剂的添加可以使青贮稻草饲料品质得到明显改善。 相似文献
46.
C.I. O''Connor-Robison MS B.D. Nielsen MS PhD R. Morris BS 《Journal of Equine Veterinary Science》2007,27(12):535-538
Six mature Arabian geldings were used in a two-period crossover study to investigate the effects of cellulase supplementation on fiber digestion. Horses were randomly assigned to either a control (CO; n = 3) or a cellulase (CE; n = 3) treatment for the first period and then treatments were switched for period 2. Each period consisted of a 10-day diet adaptation followed by a 3-day total fecal collection. The enzyme mixture contained 40,000 cellulase units/g and was fed at a rate of 3 g/day split evenly between two feedings. During the diet adaptation period, horses had ad libitum access to timothy hay and were also fed 165 g whole oats as a carrier for the supplement. When eating the CO treatment, horses consumed 16% more hay than when on the CE treatment (P = .004). Fecal output also tended to be greater when horses consumed the CO treatment as compared with CE treatment (P = .07). No differences were found between treatments for fecal percent dry matter (DM%), fecal neutral detergent fiber (NDF), fecal acid detergent fiber (ADF), fecal nitrogen (N), or fecal gross energy (GE). There was a trend for horses consuming the CO treatment to digest more NDF than when consuming the CE treatment (34.6% ± 1.5 vs 31% ± 1.5; P = .07). Horses also digested a greater %ADF, %N, and Mcal of energy when consuming the CO treatment than when consuming the CE treatment (P < .05). Cellulase addition to a hay-based horse diet decreased digestion of fiber components. 相似文献
47.
《The Journal of Applied Poultry Research》2007,16(1):150-159
The potential exists for vitamins and feed enzymes to counteract some metabolic disorders and challenges in poultry. Recent research indicates that increased levels of vitamins E and C are able to reduce ascites-related mortality in broiler chickens. Fatty liver and kidney syndrome is a nutritionally induced metabolic disorder that has been eliminated under practical production conditions due to regular supplementation of poultry diets with biotin. There is also experimental evidence for beneficial effects of 25-hydroxycholecalciferol on the incidence and severity of skeletal and performance anomalies in broilers. Another challenge is the presence of high levels of indigestible and partly soluble nonstarch polysaccharides in many cereals and other plant feed ingredients, resulting in various antinutritive effects. The use of feed enzymes, in particular endo-1,3-1,4-β-glucanase and endo-1,4-β-xylanase, to counteract these adverse effects is well implemented by the feed industry. A typical example of metabolic challenge is the reduced availability of phytate P in plant feed ingredients and the potential for phytate to act as an antinutritional component. Microbial phytase provides a practical solution for improving P availability in plant ingredients. In addition, recent studies suggest phytate can stimulate endogenous losses, and phytase can help prevent those losses. 相似文献
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49.
目的是观察鸡传染性贫血多次免疫对肝微粒体中抗氧化酶活性的影响.对20只SPF鸡随机分为2组,每组10只,免疫组鸡用鸡传染性贫血弱毒苗免疫4次,每次间隔2周,对照组鸡注射同剂量的生理盐水.最后一次免疫后10d取肝脏制备微粒体,利用测试盒测定肝微粒体中的GSH-Px活性、SOD活性、CAT活性和MDA含量.结果与对照组相比,免疫组肝微粒体中GSH Px活性、SOD活性和CAT活性都显著提高(P<0.05),MDA含量显著减低(P<0.05).结论为鸡传染性贫血多次免疫可提高鸡体抗氧化能力. 相似文献
50.
用盆栽法对原产于辽宁本溪的野菊(Dendranthema indicum)幼苗在自然干旱胁迫下的生理响应进行了研究。结果表明,渗透调节物可溶性蛋白含量处理期间一直低于对照,后期开始上升并接近对照;可溶性糖与之相反,处理前期急剧上升后期开始下降;丙二醛与相对电导率在处理期间都高于对照,随时间的延长,复水组逐渐接近对照;保护酶系统变化趋势比较一致,都是先上升后下降,最后再上升。本研究认为这种变化前期是植物自我的保护机制,后期的抗氧化酶含量上升可能是植物对环境产生了适应性。综合分析,认为在自然干旱胁迫下野菊有较好的忍耐能力、恢复能力和适应能力,具有较强的抗旱性,可作为良好的园林植物材料应用。 相似文献