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71.
72.
ROOT UV-B SENSITIVE4 (RUS4)是拟南芥DUF647蛋白家族的一个功能未知的成员。RUS1和RUS2曾经报道和根UV-B-感应途径相关联,并在拟南芥早期幼苗形态发生和发育中发挥重要作用。为了探究RUS4的分子功能,本研究对RUS4蛋白进行了亚细胞定位分析。首先,我们通过Gateway TOPO载体系统,构建了融合蛋白表达载体pMDC83-RUS4;然后,通过农杆菌介导的花苞转化法,获得了转化pMDC83-RUS4的转基因拟南芥株系;对转基因植物叶肉原生质体的荧光显微镜观察显示,RUS4-GFP信号与叶绿体的自发荧光共定位,说明RUS4蛋白定位叶绿体中。该结果为进一步研究RUS4的分子功能奠定了良好的基础。 相似文献
73.
河糯612是以自交系 HN601作为父本,与自主选择自交系 HN16作为母本,经杂交选育而成的糯玉米杂交种,于2018年5月30日通过广西壮族自治区农作物品种审定委员会审定。河糯612幼苗长势旺盛、整齐,植株穗位、株高较为整齐,果穗均匀、穗长较长,结实好,行排列整齐,行间紧致,出籽率比较高,色泽好。可在广西全区种植。 相似文献
74.
75.
Wheat grain damaged by wheat bug (Eurygaster spp.) contains the bug salivary secretion which hydrolyses the gluten needed for the dough quality of breadmaking due to its proteolytic activity. Since the protease inhibitors are widespread throughout the plant kingdom, the possibility of using some plants, especially food and feed legumes to decrease the proteolytic activity of flours milled from bug damaged wheats was investigated. The proteolytic activity was considerably inhibited by pefabloc-SC and EDTA-Na2, suggesting that bug protease(s) included serine and metallo proteases. Extracts from cones of hops, seeds of grass pea, red kidney bean and sunflower caused reduction in the activity of bug protease(s). Effects of hop extract on electrophoretic, rheological properties of high protease activity flours milled from different bread wheat cultivars damaged by the bug were also studied. The dough development time and stability values of high protease activity flours increased considerably with hop extract at the lowest addition level (10:1, flour to hop extract ratio). The doughs supplemented with the hop extract had higher maximum resistance and lower extensibility values as compared to their controls. These results suggested that hop extract had improving effects on high protease activity flours due to the bug damage. 相似文献
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77.
了解高寒地区燕麦人工草地在燕麦品种、施肥措施和混播水平下土壤碳氮储量潜力及垂直分布动态,为高寒地区燕麦人工草地建植提供理论依据。采用4个燕麦品种(A1:青燕1号,Avena sativa cv. Qingyan No.1;A2:林纳,A. sativa cv. Lena;A3:青海444,A. sativa cv. Qinghai 444;A4:青海甜燕麦,A. sativa cv. Qinghai)、4个施肥水平(B1:不施任何肥料,CK0;B2:尿素75kg/hm2+磷酸二铵150kg/hm2,IM;B3:有机肥1500 kg/hm2,OM;B4:尿素37.5 kg/hm2+磷酸二铵75 kg/hm2+有机肥750 kg/hm2,IM+OM)和4个箭筈豌豆混播水平(C1:0 kg/hm2;C2:45 kg/hm2;C3:60 kg/hm2;C4:75 kg/hm2)的三因素四水平正交试验设计[L16(45)],在燕麦拔节期、抽穗期、开花期、乳熟期和收获后期研究了3个因素对高寒区燕麦人工草地土壤C、N储量的影响极其垂直分布特征,为高寒区燕麦人工草地土壤固C、固N潜力评估提供理论依据。品种、施肥和混播均显著影响了燕麦人工草地土壤C、N储量。3个因素在作物生长期对土壤C储量的积累的影响大小表现为施肥>混播>品种,收获后期表现为混播>施肥>品种;各时期对土壤N储量的影响大小均表现为施肥>混播>品种。采用尿素37.5 kg/hm2+磷酸二铵75 kg/hm2+有机肥750 kg/hm2的施肥处理,混播75 kg/hm2箭筈豌豆建植的燕麦人工草地土壤C、N储量最高。施肥措施造成燕麦人工草地各时期不同土层间土壤C、N储量的差异。在3种措施影响下燕麦人工草地0~50cm土层土壤C、N储量潜力分别为176.78 t/hm2和11.78 t/hm2。土壤C、N随着土层的加深而逐渐下降,0~20cm土层土壤C、N储量显著高于其它土层。 相似文献
78.
Aerobic culture is a promising water-saving technology in irrigated rice ecosystems, but the vulnerability of plants to fluctuations in soil moisture constrains leaf expansion and yield. The objective of this study was to examine whether an aboveground architecture with large leaves and reduced tillering is associated with vigorous leaf growth in aerobic rice culture. In a series of field experiments, we evaluated the agronomic performance of an IR64 introgression line, YTH323 (IR84640-11-27-1-9-3-2-4-2-2-2-B), with fewer tillers and larger leaves than IR64, derived from New Plant Type rice, under various water and nitrogen conditions. In flooded culture, YTH323 yielded the same as IR64 and 38% more than IR65564-44-51 (a New Plant Type rice) (9.0 vs. 6.6 t ha−1). In aerobic culture, in contrast, it yielded 81% more than IR64 in slightly dry soils (5.1 vs. 2.8 t ha−1). YTH323 had a higher leaf area index than IR64 and IR65564-44-51 under slightly dry soil conditions and under a range of nitrogen conditions. The higher and more stable yield of YTH323 in aerobic culture was attributable to greater early vigor, high specific leaf area, a high ratio of leaf weight to total biomass, and larger leaves, along with the characteristics of high-yield cultivars such as high responsiveness to fertilizers and good grain filling. We conclude that genetic modification of the aboveground architecture of IR64, a typical tropical lowland rice cultivar, to reduce tiller and leaf number improves adaptation to aerobic culture. 相似文献
79.
80.
Legislation limiting the use of chlorpropham (CIPC), the major potato sprout suppressant, has led to a need for new technologies to extend storage life of tubers. Ultra violet C (UV-C) has been used postharvest to reduce disease incidence on many crops, yet its use and efficacy as a sprout suppressant has not been investigated. The aim of this project was to identify the optimum dose and treatment timing of UV-C treatment on potato tubers as an alternative method of sprout suppression to reduce the dependence on chemical sprout suppressants. Up to six potato cultivars over two seasons were treated with varying doses of UV-C ranging from 0 to 30 kJ m−2 either at harvest or at first indication of dormancy break. The tubers were stored at 9 °C and sprout growth and incidence assessed. Treatment with moderate UV-C doses (5–20 kJ m−2) suppressed sprout length and sprout incidence in a range of cultivars. Periderm DNA damage and programmed cell death were not detected in response to any of the UV-C doses. The inactive ABA metabolite, ABA-GE, increased in response to 10 or 20 kJ m−2 within 72 h of treatment. Multivariate analysis showed a negative relationship between ABA metabolites and sprout growth/incidence during storage. This study found that UV-C reduced sprout growth in potato with no deleterious effects on tuber quality. This suggests potential for further development as an alternative or supplement to conventional sprout suppressant technologies. 相似文献