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11.
以‘甘农3号’为研究材料,采用室外(防雨网室)盆栽营养液砂培法,模拟土壤中2种氮素形态(NO3--N和NH4+-N)的存在形式,研究混合态氮(NO3--N:NH4+-N为1:1)的5个供氮水平(0,105,210,315,420 mg·L-1)对紫花苜蓿固氮酶活性和酰脲含量的影响并筛选出最佳氮浓度;在筛选出的最佳氮浓度下研究2种形态(NO3--N和NH4+-N)氮的7种不同的配比NO3--N:NH4+-N(1/7,1/3,3/5,5/5,5/3、3/1,7/1)对紫花苜蓿固氮酶活性和酰脲含量的影响;并对根瘤固氮酶活性和酰脲含量之间的相关关系进行了分析。研究表明:最能促进紫花苜蓿酰脲累积和根瘤固氮能力的外源氮浓度为210 mg·L-1;最佳混合型态氮配比为NO3--N:NH4+-N=1/3。2种试验处理下紫花苜蓿地上部分酰脲含量(y)与固氮酶活性(x)之间分别存在显著正相关关系(P < 0.01),可分别用y=0.0321x+0.4759(R=0.911)和y=0.0313x+0.5545(R=0.960)表示,且2模型高度相似,这为寻求生产中评估紫花苜蓿固氮能力的简便方法提供理论依据。 相似文献
12.
缺磷胁迫对大豆根瘤生长和结瘤固氮的影响 总被引:3,自引:0,他引:3
采用砂培培养方法研究了缺磷胁迫对大豆根瘤生长和结瘤固氮的影响.结果表明:缺磷胁迫抑制了大豆根瘤的生长,表现为根瘤干重重下降,根瘤数量减少;缺磷胁迫显著降低了大豆的固氮能力,表现为根瘤吲氮酶活性和豆血红蛋白含量降低;缺磷胁迫降低了大豆的固氮量,固氮量随磷水平的增加而增加,随着生育时期的推进,固氮量表现为先增加后降低再增加的趋势,无磷(0μmol·L~(-1))与高磷(50 μmo·L~(-1))以及低磷(30μmol·L~(-1))与高磷处理间均达到了5%的显著差异.因此,缺磷胁迫抑制了大豆的结瘤固氮作用,使大豆的同氮效率降低. 相似文献
13.
14.
马尾松幼树根系固氮细菌的发现及其初步研究 总被引:8,自引:0,他引:8
对六类不同岩性发育的土壤中马尾松幼树根系的固氮细菌进行了初步研究,结果发现,在马尾松幼树的根系上能普遍分离到固氮细菌,但在不同岩性的土壤中分离的效果有差异;主、侧根及树势等的不同都影响分离效果。采用根段法和富集法共分离到142个菌株。其中一株经鉴定为争论产碱菌(Alcaligenesparadoxus)。并对其中26个菌株进行了回接实验,有9个菌株回接成功。固氮效能的测定结果表明其中A`10、A`13、G1、g2w四株菌的固氮效能为每消耗1g葡萄糖分别固定氮2.10—3.50mg、4.20—5.13mg、3.66—6.83mg和1.93—2.60mg。 相似文献
15.
桑树根际固氮细菌的分离鉴定及固氮酶活力测定 总被引:5,自引:1,他引:4
利用固氮细菌可降低桑园化肥使用量和提高桑叶产量与品质。采用选择性培养基,从桑树根际分离获得24个具有固氮能力的细菌分离株,以rep-PCR基因指纹分析聚类为18个聚类群。经固氮酶活性测定,PA19、PA2和PK1菌株具有较强的固氮酶活性。利用菌落形态特征观察及16S rDNA碱基序列测定和同源性分析,对3株细菌进行鉴定的结果是:PA19菌株为中慢生根瘤菌属(Mesorhizobium sp.),PA2菌株为假单胞菌属(Pseudomonas sp.),PK1菌株为土壤杆菌属(Agrobacterium sp.)。 相似文献
16.
Morphology,biomass,nitrate reductase(NR)and nitrogenase activity in Albizia chinensis(Osb.)Merr.nodules were assessed on monthly and seasonal basis for 1 year.Average NR and nitrogenase activity was higher during the rainy season,reaching a maximum in August.Thereafter,activity decreased through autumn and reached a minimum value during winter.Fresh and dry biomass of nodules increased gradually from summer to the rainy season and then started decreasing with the onset of winter as nodules began to senesce.Among four developmental stages of the nodules that correspond to their increasing age,NR and nitrogenase activity remained low in stage 1 nodules and peaked in stage 2.The activity of both enzymes further decreased with increasing age(stage 2 to stage 4).Morphological features such as shape,diameter and color varied considerably among the developmental stages.Stage 1 nodules were cream-colored,oval to heartshaped with smallest average diameter whereas at stage 2,they became bilobed to tetralobed.On the other hand,stage 3 nodules had the largest average diameter and were multilobed in structure.Stage 4 nodules that correspond to the senescing stage were dark brown to black,multilobed,flattened and hollow due to degeneration of nodular tissue. 相似文献
17.
[目的]探讨从巴西引进的甘蔗品种与广西主栽甘蔗品种在低氮条件下种植的氮代谢差异,以及这种差异与其固氮酶活性之间的关系。[方法]以巴西引进的甘蔗固氮品种和广西主栽的甘蔗品种为材料,在低氮条件下进行桶栽试验,在甘蔗的生长初期、生长盛期及生长后期分别取甘蔗品种的叶片样本分析其氮代谢和铵同化相关的酶活性和固氮酶活性,并分析各指标间的相关性。[结果]不同甘蔗品种的固氮活性不同,其总体表现为巴西引进的甘蔗品种的固氮酶活性都高于当地主栽品种;除了RB72-454品种外,几个巴西品种的硝酸还原酶活性都较高,当地甘蔗品种与巴西引进甘蔗品种的硝态氮含量和谷氨酰胺合成酶活性相差不大;固氮酶活性与几个氮代谢相关指标的相关性分析表明,除了铵态氮含量与固氮酶活性成负相关外,其他几个指标与固氮酶活性都没有太大的相关性,铵态氮对甘蔗体内的固氮酶活性有显著的抑制作用。 相似文献
18.
The Azospirillum 10SW used in our experiments was isolated from roots of wheat growing in nitrogen-poor soil of a hilly region of Nepal, where
inorganic nitrogen fertilizers were never used. The main objectives of this work were to assess the effects of inorganic nitrogen
fertilization in the yield responses of wheat grown in association with the bacteria. The in vitro experiments were done in
laboratory, whereas the pot experiments were performed in a greenhouse. The nitrogenase activities of in vitro grown Azospirillum were repressed by nitrate. The magnitude of repression was lower when the bacteria were growing in association with wheat.
The number of roots per plant was increased significantly in inoculated plants irrespective of the nitrate concentration of
the medium. Inoculation with Azospirillum 10SW also increased the yield of wheat grown in pots with medium levels of nitrogen fertilization. These data show the possibility
of inoculation of this Azospirillum spp. in combination with nitrogen fertilizer to improve the yield of wheat. Azospirillum inoculation enhanced the development of roots and shoots in the early growth stages of wheat. It may be one of the factors
responsible for the yield increases.
Received: 11 December 1996 相似文献
19.
Summary Hydrogenase activities and N2-fixing capacities of soybean nodules (Glycine max. cv. Hodgson), inoculated with strains ofBradyrhizobium japonicum andRhizobium fredii from different geographical regions, were measured after 35 days of culture under controlled conditions. Of the strains tested, 47% induced nodules with bacteroids which recycled H2. The data obtained suggest that H2-recycling ability is not a major factor influencing early N2-fixation which depends essentially on the precocity and intensity of the initial nodulation. 相似文献
20.
E. Fernández Valiente A. Quesada C. Prosperi M. Nieva F. Leganés A. Ucha 《Biology and Fertility of Soils》1997,24(4):353-357
Short- and long-term experiments were conducted in the rice fields of Valencia, Spain, to determine the ecological significance
of ammonium on nitrogen fixation. A significant inhibition of nitrogenase activity by ammonium, at concentrations higher than
0.5mM, was observed after 8h of incubation in short-term experiments done with a bloom of the N2-fixing cyanobacterium Anabaena sp. In a second set of short-term experiments for in situ assays of nitrogenase activity in the field, a significant correlation
between nitrogenase activity and the number of N2-fixing cyanobacteria in soil was found. No significant inhibition of nitrogenase activity by ammonium at concentrations up
to 2mM was observed in these assays after 24h of incubation. This lack of inhibition was probably due to the rapid decrease in ammonium
content in the flood water. Only 5% of the ammonium initially added remained in the water 24h later. In the long-term experiments,
nitrogenase activity was assayed in plots fertilized with 0, 70 and 140kgNha–1, over the cultivation cycle, for 5 years. A partial inhibition of nitrogenase activity by deep-placed N fertilizers was observed.
Differences were only significant in 2 years. Mean results from 5 years only showed significant differences between plots
fertilized with 0 and 140kgNha–1. The partial inhibition of nitrogenase activity by ammonium increased over the cultivation cycle. Inhibition was only significant
in September, at the end of the cultivation cycle.
Received: 28 January 1996 相似文献