优化提取裸花紫珠总黄酮含量及对表皮伤口易感染菌抑菌性的研究

为了选出裸花紫珠总黄酮提取的最优方法,比较了ASE、超声、微波萃取和多功能浓缩4种提取方法在裸花紫珠总黄酮提取率上的差异,并利用微孔刃天青法测定总黄酮对表皮易感染菌的MIC值。结果发现ASE法总黄酮提取率最高,约为1.26%,其次为超声和微波萃取法,总黄酮提取率分别约为1.03%和0.98%,多功能浓缩法提取率最低,约为0.36%,且耗时最长。而且研究发现,ASE的MIC值最低。最终得出ASE提取效率最高,且提取物有较好的抑菌性,是裸花紫珠总黄酮提取的最优方法。     

一类耗散非线性波动方程初边值问题整体解的存在与非存在性

本文考虑一类耗散非线性波动方程的初边值问题。用半(?)方法和先验估计论证了该问题小初值下在时间大范围的可解性、唯一性;并用凸性方法证明了(?)当条件下该问题的解在有限时间内发生爆破现象。     

建筑物的机械排烟

本文作者从实际经验出发,详细阐述了建筑物的机械排烟原理及设计施工,管理中的各项注意事项。     

Genotype evaluation,muscle histopathology and ultrastructure in a Duchenne muscular dystrophy animal model

AIM: To evaluate the genotype , muscle histopathology and ultrastructure in dko mice. METHODS: Dystrophin/Utrophin-deficient double knockouts (dko) mice were obtained from university of Oxford, UK. Genotype of filial generation of heterozygote was evaluated by PCR-SSP. HE staining and fluorescent immunohistochemistry by SABC-Cy3 were used to detect striated muscle of dko mouse, and the muscle ultrastructure was observed by transmission electron microscope(TEM). RESULTS: In 112 filial generation mice, there were 28 mdx (25.0%), 26 dko (23.2%) and 58 heterozygote (51.8%), which coincided with the law of Mendelian inheritance. HE staining showed that the myocytes were not very uniform, there were phenomenon of round outline, centrally nucleated fibers, widening interspace, inflammatory cell infiltration and connective tissue proliferation in dko mice. There were no any immunofluorescent expression of dystrophin and utrophin in sarcolemma in dko mice. TEM showed sarcolemma breakage, separation and edema, and loose myofibril texture, inflammatory cell infiltration and connective tissue proliferation in dko mice. CONCLUSION: PCR-SSP is a very quick and accurate way for genotype evaluation of filial generation. The pathophysiology of dko mouse was very similar to Duchenne muscular dystrophy (DMD), and dko mouse is an ideal animal model for study of DMD clinical therapy.     

五种豆科牧草耐盐临界值、极限值的研究

针对黄淮海２０００ｋｍ２内陆盐碱地，经２年时间，用盆栽实验方法进行研究，确定出五种豆科牧草耐盐临界值和极限值。其中保定苜蓿、猎人河苜蓿、红豆草这三种豆科牧草耐盐临界值为０．４％，极限值为０．５％。草木樨耐盐临界值为０．３％，极限值为０．４％。沙打旺耐盐临界值０．２％，极限值为０．３％。     

关于核密度具有间断点的Cauchy主值积分

讨论在实轴上核密度具有无穷个间断点的Ｃａｕｃｈｙ主值积分的性质，在一定条件下，得到了一些结果。     

水资源核算及对GDP的修正——以中国东部经济发达地区为例

对中国东部经济发达地区水资源核算的结果表明 ,以GDP为主要衡量指标的现行国民经济核算体系过高地估计了该地区经济的发展水平。扣除水资源价值损失 ,1994～ 1997年区域GDP平均下降 5 5 8% ,这意味着东部经济发达地区经济增长存在着较为严重的环境负债     

高原鼠兔对草地植被的危害及防治阈值研究

对高原鼠兔在不同时期的危害特征进行了研究，针对该鼠在补偿危害期和非补偿危害期的危害量提出了一种估算方法，得出其群体全年平均危害量β（干重）为4．94kg／只。同时根据春季灭鼠率（k）、秋季种群数量变化的预测值（λ）以及群体平均危害量（β）等参数估算可挽回损失值y。当可挽回损失值与成本参数（c）相等时，春季防治的经济阈值（x）为19．09只／hm^2或63．6个洞口／hm^2。     

烤烟适宜施氮量试验初报

试验结果表明,烤烟施氮量以90.0~112.5kg/hm2为佳,在此条件下,烤烟的产量和产值最高。