全文获取类型
收费全文 | 15960篇 |
免费 | 970篇 |
国内免费 | 1759篇 |
专业分类
林业 | 1063篇 |
农学 | 2301篇 |
基础科学 | 742篇 |
2297篇 | |
综合类 | 6733篇 |
农作物 | 1593篇 |
水产渔业 | 583篇 |
畜牧兽医 | 2006篇 |
园艺 | 631篇 |
植物保护 | 740篇 |
出版年
2024年 | 57篇 |
2023年 | 272篇 |
2022年 | 450篇 |
2021年 | 550篇 |
2020年 | 529篇 |
2019年 | 559篇 |
2018年 | 470篇 |
2017年 | 687篇 |
2016年 | 774篇 |
2015年 | 652篇 |
2014年 | 837篇 |
2013年 | 974篇 |
2012年 | 1137篇 |
2011年 | 1154篇 |
2010年 | 912篇 |
2009年 | 895篇 |
2008年 | 792篇 |
2007年 | 964篇 |
2006年 | 878篇 |
2005年 | 734篇 |
2004年 | 602篇 |
2003年 | 511篇 |
2002年 | 438篇 |
2001年 | 414篇 |
2000年 | 322篇 |
1999年 | 279篇 |
1998年 | 194篇 |
1997年 | 208篇 |
1996年 | 178篇 |
1995年 | 169篇 |
1994年 | 146篇 |
1993年 | 155篇 |
1992年 | 152篇 |
1991年 | 121篇 |
1990年 | 78篇 |
1989年 | 103篇 |
1988年 | 61篇 |
1987年 | 59篇 |
1986年 | 31篇 |
1985年 | 34篇 |
1984年 | 35篇 |
1983年 | 34篇 |
1982年 | 20篇 |
1981年 | 14篇 |
1980年 | 10篇 |
1979年 | 12篇 |
1978年 | 6篇 |
1977年 | 9篇 |
1976年 | 8篇 |
1962年 | 5篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
71.
春冠亲本之一493是将国外引进的资源96011、9502杂交后,经系统选育,采用露地越冬栽培加大耐先期抽薹性的选择强度选育出的自交不亲和系。另一亲本711是从国外引进的资源B-21经多代自交选育出的自交不亲和系。该品种耐寒,露地越冬不易先期抽薹,早熟,叶球尖桃形,肉质脆嫩,品质佳。单球质量1.5kg左右,每667m^2产量3500kg左右。在南方地区可露地越冬栽培,已在长江流域示范推广。 相似文献
72.
K.F. Stock 《Livestock Production Science》2005,92(1):77-94
The results of a standardised radiological examination of 5928 Hanoverian Warmblood horses selected for sale at auction were used to predict relative breeding values (RBV) in the 23,662 horses included in the last four generations of the probands' pedigrees. The distribution of the RBV was investigated in the whole four-generation pedigree, in the probands and in those stallions that contributed at least three offspring to the probands. The RBV of the probands' sires were further compared with the officially published performance-based relative breeding values, i.e., total indices dressage (TID) and jumping (TIJ). The sires' level of dressage indices was considerably higher (mean TID=110) than of the jumping indices (mean TIJ=98). Total indices radiographic findings (TIR) were calculated for the sires with varying weighting given to the individual RBV. In each case, this resulted in a mean TIR of 99. Finally, total indices were derived from TIR and TID and/or TIJ in order to develop different selection schemes for all-purpose breeding and for breeding focused on dressage and show jumping. All breeding values under consideration increased by between 1% and 19% when radiographic findings were weighted with between 30% and 60% complementary to the corresponding performance parameters, and when only sires were selected with above average total indices. At the same time, the prevalences of the radiographic findings investigated here were lowered by up to 10% each. When only one radiographic finding was considered at a time, the maximum attainable response to selection was a 16-23% increase in the relative breeding values and a relative decrease in prevalences of radiographic findings of between 31% and 52%. The results of this study indicate that it is possible in horse breeding to consider simultaneously health and performance traits. Medical data should be included in the prediction of breeding values in order to improve the radiological status of today's riding horses. 相似文献
73.
A general bio-economic model for beef cattle production was used to define breeding objectives for Charolais cattle to be utilized in a variety of linked production systems. Economic weights were calculated for 16 traits (some with both direct and maternal components) in three production systems (pure-breeding and terminal crossing with beef or dairy cows) and two marketing strategies (sale or fattening of weaned surplus calves). Economic weights for the total breeding objective were calculated as weighted averages, where weights were numbers of cows expected to be mated with Charolais bulls in each production system and marketing strategy. Results suggest that the direct component of calving performance and cow longevity were of primary economic importance in all systems. Conception rate of cows and weaning weight reached about 50% of the standardized economic weight of calving performance in purebred systems with sale of weaned calves, whereas in purebred systems with fattening the economic importance of the direct component of cow conception rate, losses at calving, mature weight of cows, weaning weight, and fattening traits were of equal importance (each approximately about 20% that of calving performance). In terminal crossing systems, weaning weight was important when calves were sold at weaning, and fattening traits were important for systems selling fattened animals. The bio-economic model performed well under the conditions of this demonstration and could easily be customized for other applications. 相似文献
74.
茭白主茎地上部养分积累和转运规律 总被引:1,自引:0,他引:1
以两个有代表性的茭白品种为试材,测定了植株主茎生育过程中地上各部位干、鲜质量的积累及叶片净光合速率的变化。结果表明在田间封行前,植株主茎叶和短缩茎的干、鲜质量持续缓慢增加;封行后至肉质茎快速膨大前,短缩茎的物质积累超过叶片和叶鞘;肉质茎快速膨大期间,干物质积累主要在其前期,同时短缩茎等其余部位干、鲜质量明显下降;之后肉质茎干、鲜质量增加趋缓,叶片、叶鞘质量仍持续下降,而短缩茎质量回升,进入新一轮物质积累期;主茎的总干物质量在封行后迅速提高,在肉质茎充分膨大后下降。功能叶片净光合速率在生育进程中总体呈持续缓慢下降的趋势,肉质茎快速膨大期间迅速大幅上升,紧接着又快速回落。 相似文献
75.
AIM:To establish cell line FL-POLκ- and to study the role ofPOLκ(polymerase kappa) on genetic stability.METHODS:A mammalian expression vector expressing antisense POLκ gene fragment pMAMneo -amp--POLκ was constructed by cloning the 1 690-1 918 fragment of POLκ gene into the mammalian expression vector pMAMneo-amp- in antisense orientation. FL cells were fransfected with this antisense RNA expressing vector and selected by G418. Based on the shuttle-plasmid pZ189, the mutation assay was made.RESULTS:The spontaneous mutation frequency of supF tRNA gene in the plasmid replicated in the FL- POLκ- was 11.2×10-4, while it was 4.9×10-4 and 3.7×10-4 in the control cells FL and FL-M, respectively.CONCLUSION: POLκ playes an important role in maintenance of genetic stability. 相似文献
76.
AIM:To manufacture recombinant protein of the highly conserved domain in human bone morphogenetic protein-1(BMP-1) using gene engineering methods as antigen for making wide spectrum antibody to BMP-1.METHODS:We analyzed the gene sequences and protein structures of BMP-1 and its related proteins, and chose a highly conserved fragment as target gene. Total RNA was prepared from human osteosarcoma cell line Saos-2, then the target gene was amplified with RT-PCR. The PCR product was cloned into prokaryotic expression vector pMAL c2 to get recombinant vector BMP-1(322-588aa)-pMAL c2. After transforming the recombinant plasmid into DH5-alpha and screening, several prositive clones were got for sequencing. Finally the transformed cells was induced with IPTG to get fusion protein.RESULTS:The BMP-1 gene fragment was successfully cloned into vector pMAL c2, and was able to express efficiently with IPTG inducement. The amount of expressed fusion protein is about 66%-72% in total volume of bacterial proteins.CONCLUSIONS:The recombinant protein contains several key domains(2 CUB domains and 1 EGF domain), which are shared by BMP-1 and its related proteins. Specific wide spectrum antibody to human BMP-1 and its related proteins may be generated with this recombinant protein antigen. 相似文献
77.
AIM and METHODS:To analysis the factor that involved in renal carcinogenesis, we used the bait gene AK001518 to screen GenBank. To understand the relationship between cell cycle related gene(CCRG) and p15, we did RT-PCR and Northern Blot experiments. Then we examined CCRG expression level in renal carcinogenesis. RESULTS:Gained a function unknown gene CCRG that was 67% a mino acid identical with the gene AK001518 that was regulated by p15. It was shown that the CCRG mRNA was dramatically decreased when p15 gene was over-expressed. CCRG expression level was much higher in tumor tissues and cells than normal tissues and cells. CONCLUSION:The novel gene CCRG expressed highly in the renal carcinoma, which might play a significant role in the renal carcinogenesis. 相似文献
78.
AIM:To investigate the expression of soluble guanylate cyclase protein and its mRNA in rat pulmonary artery after exposure to hypoxia and hypercapnia.METHODS:Male Sprague-Dawley rats were randomly split into 4 group, which were hypoxic hypercapnic (HH 1 week, HH 2 weeks, HH 4 weeks) group and control group, to copy pulmonary hypertensive animal model. The expression of sGCα1 and β1 subunits protein of medial and small pulmonary artery was performed by immunohistochemistry with a polycolonal antibody. In situ hybridization was performed on the rat lung tissue using sGC oligonuclear probe to assay the expression of sGCα1subunit mRNA.RESULTS:The sGCα1 and β1 subunits protein and sGCα1 subunit mRNA were faint staining in the pulmonary small and medium artery in HH1 week, HH 2 weeks and HH 4 weeks groups compared with control group (all P<0.01).CONCLUSION:sGC subunit mRNA and protein expression in pulmonary small and medium artery were decreased after exposure to hypoxia and hypercapnia, which took part in the development of the pulmonary hypertension. 相似文献
79.