全文获取类型
收费全文 | 3693篇 |
免费 | 179篇 |
国内免费 | 307篇 |
专业分类
林业 | 172篇 |
农学 | 480篇 |
基础科学 | 31篇 |
115篇 | |
综合类 | 1504篇 |
农作物 | 566篇 |
水产渔业 | 38篇 |
畜牧兽医 | 150篇 |
园艺 | 365篇 |
植物保护 | 758篇 |
出版年
2024年 | 12篇 |
2023年 | 65篇 |
2022年 | 108篇 |
2021年 | 99篇 |
2020年 | 92篇 |
2019年 | 99篇 |
2018年 | 84篇 |
2017年 | 80篇 |
2016年 | 144篇 |
2015年 | 155篇 |
2014年 | 194篇 |
2013年 | 198篇 |
2012年 | 214篇 |
2011年 | 253篇 |
2010年 | 198篇 |
2009年 | 213篇 |
2008年 | 200篇 |
2007年 | 200篇 |
2006年 | 170篇 |
2005年 | 151篇 |
2004年 | 143篇 |
2003年 | 132篇 |
2002年 | 108篇 |
2001年 | 122篇 |
2000年 | 95篇 |
1999年 | 85篇 |
1998年 | 74篇 |
1997年 | 67篇 |
1996年 | 61篇 |
1995年 | 61篇 |
1994年 | 37篇 |
1993年 | 43篇 |
1992年 | 36篇 |
1991年 | 53篇 |
1990年 | 40篇 |
1989年 | 22篇 |
1988年 | 29篇 |
1987年 | 8篇 |
1986年 | 6篇 |
1985年 | 2篇 |
1984年 | 5篇 |
1983年 | 2篇 |
1982年 | 3篇 |
1981年 | 2篇 |
1980年 | 3篇 |
1978年 | 2篇 |
1976年 | 1篇 |
1963年 | 1篇 |
1962年 | 1篇 |
1955年 | 4篇 |
排序方式: 共有4179条查询结果,搜索用时 187 毫秒
31.
测定了6个Bacillus spp.和1个Pseudomonas sp.P-13(G-对照菌株)在PPM培养基上的相互作用。在总共42个组合中,有27个组合是可亲和性的。在以B5423为单剂之一的5个组合中,亲和性的组合有4个。在温室条件下通过分批播种,在水稻分蘖盛期喷以B5423-R(B5423的标记菌株,是利福平抗性的突变体)为单剂之一的4个亲和性组合的菌悬浮液,以检测该组合中B5423-R在水稻茎基部的种群数量。结果发现,与单剂B5423-R相比,在应用1~7天内,组合B5423-R B45中,B5423-R的群体数量显著性降低;组合B5423-R B95、B5423-R B77中,B5423-R的群体数量与单剂无显著差异;组合B5423-R B4313中,B5423-R的群体数量在应用1~4天内与单剂无显著性差异,但在应用第7天显著地高于单剂。离体叶片纹枯病痛斑面积的抑制试验表明,与各自的单剂相比,组合B95 B5423及B95 Er77显著地抑制了纹枯病病斑面积;而组合B45 B80、B45 B5423和B45 B95纹枯病病斑面积显著地高于各自的单剂;其余10个组合与各自的单剂无显著差异。 相似文献
32.
哈茨木霉几丁质酶诱导及其对水稻纹枯病菌的拮抗作用 总被引:17,自引:0,他引:17
在实验室条件下分别以几丁质和水稻纹枯病菌(Rhizoctonia solani)细胞壁作唯一碳源诱导哈茨木霉(Trichoderma harzianum)菌株NF9、TC3和P1产生几丁质酶,用硫酸铵沉淀法制备几丁质酶粗提液。上述木霉菌株内切几丁质酶活性(对胶体几丁质浑浊度的减少率)分别为79.8%、74.4%和76.0%,均显著高于非诱导的阳性对照。培养第5 d几丁质诱导的木霉菌株NF9和TC3内切几丁质酶活性显著高于由水稻纹枯病菌细胞壁诱导的酶活性。体外测定表明,通过诱导的木霉菌株TC3、NF9和P1几丁质酶粗提液对水稻纹枯病病菌的拮抗圈直径可达38、21和23 mm,与非诱导的阳性对照比较有显著性差异。对木霉几丁质酶拮抗作用的特点及生防应用进行了讨论。 相似文献
33.
梨火疫细菌实时荧光PCR和诱捕PCR-ELISA检测方法的建立 总被引:8,自引:1,他引:8
根据梨火疫细菌中独特而保守存在的质粒pEA29,设计了1对引物和3条探针,建立了实时荧光PCR检测方法和诱捕PCR-ELISA检测方法。实时荧光PCR采用带荧光标记的核酸杂交探针,边扩增边检测,步骤简单,不需PCR后处理,可避免假阳性和交叉污染;诱捕PCR-ELISA检测方法只需简单处理的样品就能检测,减少了核酸不纯出现的漏检,由于增加了核酸杂交探针,可不需凝胶电泳EB染色检测,不会出现假阳性问题。 相似文献
34.
中国18 省市番茄晚疫病菌生理小种的鉴定 总被引:26,自引:2,他引:26
利用5 个含有不同单显性抗番茄晚疫病基因的番茄材料Ts19、Ts33、W1Va700、LA1033 和L3708为鉴别寄主, 对我国18 省、市、自治区的番茄晚疫病菌201 个纯化分离物进行了生理小种鉴定。结果共鉴定出8 个小种, 即生理小种T0、T1、T1 ,2 、T1 ,2 ,3 、T1 ,2 ,3 ,4 、T1 ,4 、T1 ,2 ,4 和T3。其中, 小种T1 和T1 , 2是主流小种, 地理分布最广和发生频率最高, 分别在13 和11 个省市出现, 占样本总数的28.8 %和28.4 %;其次是小种T0 和T1 ,2 ,3 , 分别在8 和7 个省市出现, 分别占1819 %和819 %; 再其次是小1 ,2 ,3 ,4 , 在4个省市有发生, 占710 %; 小种T1 ,2 ,4 、T1 ,4 和T3 分布均少, 仅在1~2 个省市发生, 分别占3.0 %、2.5 %和2.5 %。这是我国的首次报道, 为番茄晚疫病的抗源材料筛选和抗病育种提供了病理学依据。 相似文献
35.
红掌细菌性疫病的病原菌初步鉴定 总被引:3,自引:1,他引:3
在云南西双版纳的红掌上发现一种由细菌侵染引起的病害,从叶片的病组织上分离到具有致病性的杆状细菌,将分离的病原菌接种于健康的红掌上,表现出与田间一致的症状。感病初期在叶缘或叶脉间出现水渍状小点,后期病斑多呈棕褐色至黑色坏死,病健交界处多呈黄色。从接种发病的病斑与田间标样上分离的病原菌菌落形态完全相同。通过菌株的培养性状、常规生理生化特性及透射电镜下菌体形态观察结果,将病原菌初步鉴定为黄单胞菌属(Xanthomonas)。BIOLOG鉴定和致病性测定结果进一步鉴定病原菌为地毯草黄单胞菌花叶万年青致病变种Xanthomonas axonopodis pv. dieffenbachiae(Xad)(McCulloch & Pirone) Vauterin et al. 相似文献
36.
37.
38.
Thomas Miedaner Angela G. Schilling Geiger 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(1):63-70
Fusarium culmorum is a phytopathogenic, toxigenic fungus causing seedling diseases, foot rot and head blight of cereals. For estimating competition effects in mixtures of two single-spore isolates, two winter rye single crosses were tested with either four isolates individually or four 1 : 1 mixtures of the same isolates in six field environments. Two isolates (FC46, FC64) were highly aggressive deoxynivalenol (DON) and 3-acetyl DON-producers, the other two (FC30, FC71) were medium aggressive nivalenol-producers. Rye heads were inoculated during flowering with conidia of pairs of isolates expressing similar (FC46 + FC64, FC30 + FC71) or contrary (FC46 + FC71, FC30 + FC64) levels of aggressiveness and similar or different concentrations and chemotypes of mycotoxins, respectively. Head blight rating and yield components relative to the non-inoculated plots were recorded as aggressiveness traits. Additionally, mycotoxin concentrations were measured in the rye grain. Random pathogen samples were re-isolated from heads at the onset of symptom development and analysed by molecular markers (RAPD–PCR) in one environment. Aggressiveness of the isolate mixtures was significantly lower than that of the isolates applied individually on both rye genotypes. Similarly, mycotoxin concentrations were significantly lower in the mixtures in seven out of eleven comparisons. Among the re-isolates, the component genotypes of a mixture significantly deviated from the inoculated 1 : 1 ratio when a particular isolate (FC46) was present in the mixture. This isolate displayed a superior competitive ability irrespective of the aggressiveness or mycotoxin profile of the mixing partner illustrating that pathogenic fitness is caused by additional factors that have not, as yet, been identified. 相似文献
39.
Repression of deoxynivalenol accumulation and expression of Tri genes in Fusarium culmorum by fungicides in vitro 总被引:1,自引:0,他引:1
A defined medium was developed in which to monitor deoxynivalenol (DON) accumulation, fungal growth and expression of genes involved in trichothecene biosynthesis (designated Tri genes). In liquid culture, DON accumulated shortly after maximum expression of Tri6 and coincident with expression of Tri5. This was generally 96 h after inoculation. The effects of sublethal concentrations of the fungicides azoxystrobin, trifloxystrobin, kresoxim-methyl and tebuconazole on biosynthesis of the trichothecene DON by Fusarium culmorum were studied using this medium. The strobilurin fungicides trifloxystrobin and azoxystrobin significantly reduced the accumulation of DON in culture medium at a range of concentrations. Kresoxim-methyl, also a strobilurin, and tebuconazole, a triazole, did not significantly reduce the accumulation of DON, although levels were lower than those in nonamended cultures. Trifloxystrobin significantly reduced the accumulation of DON when added to cultures before initiation of trichothecene biosynthesis. RT-PCR assays of the expression of Tri6 and Tri5 genes indicated that trifloxystrobin acted by inhibiting the initiation of trichothecene biosynthesis. 相似文献
40.
The population of Phytophthora infestans in Brazil consists of two clonal lineages, US-1 associated with tomatoes and BR-1 associated with potatoes. To assess whether host specificity in these lineages resulted from differences in aggressiveness to potato and tomato, six aggressiveness-related epidemiological components – infection frequency (IF), incubation period (IP), latent period (LP), lesion area (LA), lesion expansion rate (LER) and sporulation at several lesion ages (SSLA) – were measured on detached leaflets of late blight-susceptible potato and tomato plants. Infection frequency of US-1 was similar on potato and tomato leaflets, but IF of BR-1 was somewhat reduced on tomato. Incubation period was longer on both hosts with US-1, although this apparent lineage affect was not significant. Overall there was no host effect on IP. On potato, BR-1 had a shorter LP (110·3 h) and a larger LA (6·5 cm2 ) than US-1 (LP = 162·0 h; LA = 2·8 cm2 ). The highest LER resulted when isolates of BR-1 (0·121 cm2 h−1 ) and US-1 (0·053 cm2 h−1 ) were inoculated on potato and tomato leaflets, respectively. The highest values of the area under the sporulation capacity curve (AUSC) were obtained for isolates of US-1 inoculated on tomato leaflets (6146) and for isolates of BR-1 on potato leaflets (3775). In general, higher values of LA, LER, SSLA and AUSC, and shorter values of LP were measured when isolates of a clonal lineage were inoculated on their original host than with the opposite combinations. There is evidence that there are quantitative differences in aggressiveness components between isolates of US-1 and BR-1 clonal lineages that probably contribute to host specificity of P. infestans populations in Brazil. 相似文献