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41.
To study the effects of light, external nutrition, and hormones on the color changes of Aeonium aeonium "Halloween" leaves. In this study, the 1-year-old stem cuttings of the Aeonium aeonium "Halloween" were used as materials. The leaves were sprayed with 25mg/L methyl jasmonic acid, 3‰ potassium dihydrogen phosphate, and 1‰ calcium magnesium boron mixture for two consecutive times, with an interval of 3 days. The experiment consisted of CK (water control), LS, DS (dark culture for 1 week). After 1 week of treatment, the leaf color parameters and anthocyanin pigment content were measured every 7 days for 6 consecutive weeks. The results showed that the treatment time and method had a significant impact on the leaf color parameters. In terms of brightness value L, as the treatment time extended, all three treatments showed an upward downward upward trend. Among them, the highest value was reached after 2 weeks of treatment, gradually decreased after 3 weeks, and sharply increased again after 6 weeks; In terms of red green saturation a value, after 2-4 weeks of treatment, both LS and DS treatments were significantly higher than CK, and the effect significantly decreased after 5 weeks; Light has a significant impact on a value, with DS treatment showing a significant decrease in a value after one week of shading, and a sharp increase after re lighting; In terms of the b value of yellow and blue saturation, the change in CK value is relatively small with the extension of treatment time, while the fluctuation in LS and DS treatment is significant; In terms of color saturation c value, DS treatment significantly increased with the prolongation of treatment time, and there was no significant change in LS and CK treatment within 1-3 weeks. After 3 weeks of treatment, there was no significant difference between the three treatments; With the extension of treatment time, the anthocyanin content of each treatment showed a trend of first increasing and then decreasing. After 2-3 weeks of treatment, the anthocyanin content was higher, and gradually decreased after 4 weeks of treatment; Correlation analysis shows that anthocyanin content is highly significantly positively correlated with a and c values, and anthocyanin content is a key factor in the red color of Aeonium aeonium "Halloween". 相似文献
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规模猪场4大疫病的血清学检测及免疫抑制病因分析 总被引:4,自引:0,他引:4
选取杭州、绍兴、嘉兴和湖州4个地区的规模猪场为试验猪场,用正向间接血凝试验(IHA)检测猪瘟病毒(CSFV)和口蹄疫病毒(FMDV)抗体合格率;用酶联免疫吸附试验(ELISA)检测猪繁殖与呼吸综合征病毒(PRRSV)和猪圆环病毒2型(PCV2)抗体阳性率,分析免疫抑制状况。同时,采取临床可疑病料,用RT-PCR技术检测PRRSV、PCV2、CSFV和伪狂犬病毒(PRV)的感染率。结果显示:不同年龄猪群中,PCV2抗体阳性率都在96.0%以上,而CSFV、FMDV抗体合格率及PRRSV抗体阳性率呈现一致的变化趋势,即母猪最高,哺乳仔猪其次,培育猪和肥育猪最低;在病原检测中,PRRSV和PCV2检出率最高,分别为44.3%和60.7%。另外,双重感染和多重感染明显,特别是PRRSV+PCV2+CSFV三重感染,阳性率达7.6%。本试验结果为制定有效的免疫程序等防治措施提供科学依据。 相似文献
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黄土丘陵区不同植被类型根际微生物群落功能多样性研究 总被引:4,自引:0,他引:4
为了解生态恢复过程中土壤微生物功能特征,采用Biolog-Eco微平板法分析了黄土丘陵区人工灌木(柠条 Caragana korshinskii、沙棘 Hippophaer rhamnoides)、人工草地(沙打旺 Astragalus adsurgens、柳枝稷 Panicum virgatum)和天然草地(阿尔泰狗哇花 Heteropappus altaicus、茵陈蒿 Artemisia capillaries)的根际微生物碳源代谢多样性特征。结果表明:柠条、阿尔泰狗哇花和茵陈蒿的根际微生物对糖类的代谢能力显著高于沙棘、沙打旺和柳枝稷;阿尔泰狗哇花和茵陈蒿对氨基酸类、酚酸类和聚合物类的代谢能力较高。茵陈蒿和阿尔泰狗哇花的微生物代谢功能多样性高于其他4种植物。柠条、阿尔泰狗哇花和茵陈蒿具有相似的根际微生物代谢功能。黄土丘陵区植被类型显著影响微生物群落碳源利用能力,天然草地根际微生物群落代谢功能多样性优于人工灌木和人工草地。 相似文献
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Kaiping Deng Steffen Uhlig Laura B. Goodman Hon S. Ip Mary Lea Killian Sarah M. Nemser Jodie Ulaszek Shannon Kiener Matthew Kmet Kirstin Frost Karina Hettwer Bertrand Colson Kapil Nichani Anja Schlierf Andriy Tkachenko Mothomang Mlalazi-Oyinloye Andrew Scott Ravinder Reddy Gregory H. Tyson 《Journal of veterinary diagnostic investigation》2022,34(5):825
The COVID-19 pandemic presents a continued public health challenge. Veterinary diagnostic laboratories in the United States use RT-rtPCR for animal testing, and many laboratories are certified for testing human samples; hence, ensuring that laboratories have sensitive and specific SARS-CoV2 testing methods is a critical component of the pandemic response. In 2020, the FDA Veterinary Laboratory Investigation and Response Network (Vet-LIRN) led an interlaboratory comparison (ILC1) to help laboratories evaluate their existing RT-rtPCR methods for detecting SARS-CoV2. All participating laboratories were able to detect the viral RNA spiked in buffer and PrimeStore molecular transport medium (MTM). With ILC2, Vet-LIRN extended ILC1 by evaluating analytical sensitivity and specificity of the methods used by participating laboratories to detect 3 SARS-CoV2 variants (B.1; B.1.1.7 [Alpha]; B.1.351 [Beta]) at various copy levels. We analyzed 57 sets of results from 45 laboratories qualitatively and quantitatively according to the principles of ISO 16140-2:2016. More than 95% of analysts detected the SARS-CoV2 RNA in MTM at ≥500 copies for all 3 variants. In addition, for nucleocapsid markers N1 and N2, 81% and 92% of the analysts detected ≤20 copies in the assays, respectively. The analytical specificity of the evaluated methods was >99%. Participating laboratories were able to assess their current method performance, identify possible limitations, and recognize method strengths as part of a continuous learning environment to support the critical need for the reliable diagnosis of COVID-19 in potentially infected animals and humans. 相似文献
48.
赤峰地区敖汉苜蓿冻害及其防御技术 总被引:15,自引:1,他引:15
敖汉苜蓿在赤峰地区受冻害程度有4种类型;全株未受冻;根颈上端受冻受黑,下端仍能再生新芽;全部根颈和根的部分受冻变黑,已不能再生新芽;全株受冻死亡。引起冻害的主要原因是播种期偏晚,在播种当年生长期短,根颈入土浅。结果表明,越冬前苜蓿必须具有2个以上分枝,根颈入土深>5cm以上,根颈膨大,直径>0.3cm以上者方能减轻或避免受冻。适时早播,施种肥,延长生长期促进幼苗生长,在封冻前对平作进行浅耕覆土或深开沟磨平等措施,均能增加根颈保温层,有效提高苜蓿越冬率。 相似文献
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