Global status of nematode problems of groundnut, pigeonpea, chickpea, sorghum and pearl millet, and suggestions for future work

Analysis of responses received from 40 cooperators in 20 countries to a questionnaire on nematode problems of groundnut, pigeonpea, chickpea, sorghum and pearl millet suggested that Meloidogyne spp. are internationally important nematode pests of groundnut, chickpea and pigeonpea. Pratylenchus spp. are important on all the five crops. In India, Heterodera cajani and Rotylenchulus reniformis are important pathogens of pigeonpea. Over the last 10–15 years, extensive nematode disease surveys have been undertaken for these crops in Australia, Egypt, India, Jamaica, Senegal, Sudan, Thailand and Zimbabwe; however, < 10% of the total crop areas were covered by these surveys. Except for Brazil, Egypt, USA and Zimbabwe, growers do not use nematicides to control the nematodes. Cultural practices, especially crop rotations, are the most commonly used control measures. Species of Meloidogyne, Pratylenchus and Rotylenchulus on the legumes, and species of Hoplolaimus, Pratylenchus, Quinisulcius and Xiphinema on the cereals, are strongly suspected of increasing the severity of fungal diseases. Work aimed at finding host resistance is being done in Brazil, Fiji, India and the USA, and some sources of resistance have been identified against Meloidogyne arenaria, M. incognita, M. javanica and R. reniformis. Facilities for resistance screening work now exist in many countries. Information on damage thresholds of important pest species are available only from Brazil, Fiji, India and the USA.     

XIP-type endoxylanase inhibitors in different cereals

Highly pure XIP-type (for Xylanase Inhibiting Protein) endoxylanase inhibitor fractions were selectively obtained with a high yield from rye, durum wheat, barley, and maize extracts by affinity chromatography with immobilised Aspergillus niger endoxylanase Xyn1 following removal of the TAXI-type (for Triticum aestivum xylanase inhibitor) endoxylanase inhibitors by affinity chromatography with immobilised Bacillus subtilis endoxylanase XynA. No inhibitors belonging to the XIP family occur in rice, oats, and buckwheat. N-terminal amino acid sequences of the non-wheat XIP-type inhibitors were very similar or identical to those of wheat XIP-1, a chitinase homologue. The isolated inhibitors are basic, monomeric proteins of ca. 30 kDa with pI values of at least 8.5 (rye, durum wheat, and barley XIP) and ca. 7.0 (maize XIP). They are, in general, active against fungal endoxylanases and do not hydrolyse chitin. SDS–PAGE analysis and high-resolution cation exchange chromatography suggest the presence of multiple XIP-type isoinhibitors in the different cereals.     

青岛农业大学《粮油食品工艺学》实验与实习教学的改革

粮油食品工艺学为食品科学与工程专业的主要专业课,传统的粮油食品工艺学的实验和实习教学中存在许多问题,不利于学生能力的培养。本文就粮油食品工艺学实验与实习教学的改革措施进行了论述。     

Fractionation of cereal flour by sedimentation in non-aqueous systems. II. Rheological characterisation and baking performance of the protein fraction

A previously described method for the non-aqueous fractionation of cereal flours by sedimentation in non-aqueous solvents was carried out using flours of three wheat cultivars differing in baking performance, as well as one rye and one barley flour. The method was based on differences in the densities of starch (higher) and protein (lower). Thus, suspending finely milled flour in an inert solvent mixture with a density in between the densities of starch and protein yielded a sedimented starch fraction and a protein-rich fraction at the surface of the solvent. Further purification of this upper fraction provided a protein fraction, a middle fraction, and a lipid fraction. The protein fractions were examined by means of rheological methods such as micro-extension tests and creep-recovery tests. They also were reconstituted to standard flour with a protein content of 13.5%, which was used for micro-scale baking tests. Compared to aqueous isolated gluten, the hydrated protein fractions from wheat were much more extensible and had a lower resistance to extension. The baking performance of the wheat protein fractions was superior to gluten and comparable to the native wheat flours. The protein fraction from rye gave a wheat-like bread crumb, whereas the barley protein was not suited for bread making.     

谷物中柠檬黄和日落黄高效液相色谱检测方法的优化

[目的]探索利用高效液相色谱检测人工色素的优化方法。[方法]以高效液相色谱方法梯度洗脱,分离、分析谷物中的柠檬黄和日落黄,对前处理温度、超声时间、柱温、梯度洗脱条件进行考察,确定最佳检测条件。[结果]在样品前处理温度40℃下,最佳色谱分析条件为流动相为含pH为6.8的醋酸-醋酸铵(0.02 mol/L)的甲醇/水混合液、柱温35℃,梯度洗脱条件甲醇:20%～35%,3%/min;35%～98%,6%/min;98%继续8 min。采用HPLC优化法测定谷物中柠檬黄和日落黄含量的精密度良好,二者平均加样回收率分别为97.9%和100.2%,最低检出限分别为0.02、0.03 mg/kg。[结论]该方法缩短了分析时间,提高了检测灵敏度和人工色素的分离度。     

轴流脱粒空间谷物运动仿真研究

运用变质量系统的基本原理,建立了轴流脱粒空间内谷物运动的非线性数学模型,编制了相应的ＭＡＴＬＡＢ仿真程序。通过计算机动态仿真,得到了更加接近实际的季节性位移图和速度图,并对其进行了深入的理论分析和验证。研究思想突破了传统的轴流脱粒理论,为脱粒机理研究开辟了新途径。     

禾谷类作物的比较基因组研究

 水稻是基因组最小的禾谷类作物,饱和遗传连锁图谱的构建,以及在此基础上开展的标记辅助选择和抗病基因克隆,表明水稻基因组研究已经领先于其他禾谷类作物。比较基因组研究表明：小麦、玉米、高粱、谷子和甘蔗的基因组均可由水稻染色体区段重新排列而成，这些区段上DNA标记的排列顺序在各个种之间保留。各种作物基因组大小的差异可能由于各个区段内基因间重复顺序扩增的程度不同所致。根据这些区段在各种作物染色体的排列顺序，有人提出根据水稻染色体区段排列单个原始禾谷类染色体的设想，为深入研究禾谷类作物的进化遗传提出了全新的思路。禾谷类作物基因组之间的共线性有利于在小基因组内克隆大基因组作物的同源基因，使生物技术在作物育种中发挥更大的作用。     

Automated Crop and Weed Monitoring in Widely Spaced Cereals

An approach is described for automatic assessment of crop and weed area in images of widely spaced (0.25 m) cereal crops, captured from a tractor mounted camera. A form of vegetative index, which is invariant over the range of natural daylight illumination, was computed from the red, green and blue channels of a conventional CCD camera. The transformed image can be segmented into soil and vegetative components using a single fixed threshold. A previously reported algorithm was applied to robustly locate the crop rows. Assessment zones were automatically positioned; for crop growth directly over the crop rows, and for weed growth between the rows. The proportion of crop and weed pixels counted was compared with a manual assessment of area density on the basis of high resolution plan view photographs of the same area; this was performed for views with a range of crop and weed levels. The correlation of the manual and automatic measures was examined, and used to obtain a calibration for the automatic approach. The results of mapping of a small field, at two times, are presented. The results of the automated mapping appear to be consistent with manual assessment.     

Water-use efficiency of wheat-based rotation systems in a Mediterranean environment

Crop production in Mediterranean-type environments is invariably limited by low and erratic rainfall (200-600 mm year⁻¹), and thus soil moisture, and by high evapotranspiration resulting from high temperature. Consequently, a major research challenge is to devise cropping systems that maximize water-use efficiency (WUE). In a long-term trial in northern Syria (1986-1998) we compared the effects of seven wheat-based rotations on soil water dynamics and WUE in both the wheat and non-wheat phase. The cropping systems were durum wheat (Triticum turgidum L.) in rotation with fallow, watermelon (Citrullus vulgaris), lentil (Lens culinaris), chickpea (Cicer arietinum), vetch (Vicia sativa), medic pasture (Medicago spp.), and wheat. Seasonal recharge/discharge were identified using the neutron probe. Depth of wetting varied with seasonal rainfall (233-503 mm). Based on crop yields, WUE was calculated for each cropping option in relation to the durum wheat crop.The greatest limitation to growth was the supply of water and not the soil moisture storage potential. Wheat grain yield was dictated by the extent to which the alternative crops in the rotation dried out the soil profile, in addition to seasonal rainfall and its distribution. Chickpea and medic extracted as much water as continuous wheat. Wheat after these crops was solely dependent on current seasonal rainfall, but fallow, lentil, watermelon, and vetch did not deplete soil moisture to the same extent, leaving some residual soil moisture for the succeeding wheat crop. This difference in soil water resulted in a significant difference in wheat yield and hence WUE, which decreased in the following crop rotation sequence: fallow, medic, lentil, chickpea, and continuous wheat. However, on the system basis, the wheat/lentil or wheat/vetch systems were most efficient at using rainfall, producing 27% more grain than the wheat/fallow, while the wheat/chickpea system was as efficient as wheat/fallow system, with continuous wheat being least efficient. With N added to the cereal phase, system WUE of the system increased, being least for continuous wheat and greatest for wheat/lentil. Wheat-legume rotation systems with additional N input in the wheat phase not only can maintain sustainable production system, but also are more efficient in utilizing limited rainfall.     

Gene flow from transgenic wheat and barley under field conditions

In this study the frequency and distance of gene flow from transgenic wheat (Triticum aestivum) and barley (Hordeum vulgare) to non-transgenic wheat and barley crops was investigated under local field conditions. Trials were conducted in the Australian Capital Territory (ACT) and in South Australia (SA). Gene flow from transgenic wheat was confirmed at frequencies of 0.012% and 0.0037% in the ACT and SA, respectively. In both trials gene flow occurred over distances of less than 12 m. Gene flow was also detected from transgenic barley at a frequency of 0.005%, over a distance of less than 12 m. The results show that under Australian field conditions, gene flow occurs at extremely low frequencies and over very short distances. Physical separation of transgenic and non-transgenic cereal crops by greater than 12 m should ensure that contamination of adjacent non-transgenic cereal crops remains less than 0.02%, well below the level permitted under Australian regulations.