芽孢杆菌碱性蛋白酶工业废水对河流的污染与防治

用从碱性蛋白酶工业废水的受纳水体中检出废水原有芽孢杆菌的方法，进行废水芽孢杆菌对水体污染度分析。结果表明，污染面主要在受纳水体下游，与上游相比其芽孢杆菌数、细菌总数、非离子氨、ＢＯＤ５、ＣＯＤｃｒ分别高１０．２１、１．７１、９．０、２．６９、０．５５倍，溶解氧降低１／４，采用碱式氯化铝和聚丙烯酰胺的絮凝处理工艺，除菌率达９９％以上。     

木瓜蛋白酶水解饲料蛋白的最佳作用条件

就木瓜蛋白酶对鱼粉，菜籽粕，豆饼，玉米，小麦麸，米糠等六种饲料蛋白水解作用条件进行了比较系统的研究。结果表明，该酶对６种饲料蛋白水解的最适ｐＨ在７．０附近，最适温度为７０℃左右。此外还测定了酶，饲料不同比例的最适作用时间和它对各种饲料的表观消化率。     

钙激活酶激活蛋白的研究进展

钙激活酶激活蛋白是钙蛋白酶的正调节因子,是其最直接的调节者。本文就钙激活酶激活蛋白的研究历史、现状发展以及意义详细论述,重点论述了其结构和功能及在肉嫩化中的作用机理。     

钙蛋白酶在骨胳肌生长及期嫩化作用中的研究发展

大量的研究表明钙蛋白酶在蛋白质降解方面起着重要作用,为了提高骨骼肌的生长速度和家畜宰后的肉品质,人们对钙蛋白酶系统的作用机理进行了大量研究,发现钙蛋白酶系统中的多种钙蛋白酶与骨骼肌生长及宰后嫩化有很大的相关性.本文就钙蛋白酶在骨骼肌生长及其嫩化中的作用的研究作一概述.     

不宜在2小时内同吃的食物

例：豆浆与鸡蛋豆浆中含有胰蛋白酶抑制物，它能抑制人体蛋白酶的活性，影响人体对蛋门质的消化和吸收，鸡蛋的蛋清里含有粘性蛋白，可以同豆浆中的胰蛋白酶结合，使蛋白质的分解受到阻碍，从而降低人体蛋向质的吸收率。虾、蟹与维生素虾、蟹等食物中含有五价砷化合物，如果与含有维生素C的生果同食，会令砷发生变化，     

猪钙蛋白酶Ⅱ基因PCR-RFLP分析及其对肉质性状的影响

本试验测定了350头F2代杂种猪(长白猪×蓝塘猪)的6个肉质性状和7个胴体性状,用PCR-RFLP方法检测钙蛋白酶Ⅱ(CalpainⅡ)基因型,分析猪CalpainⅡ基因型间肉质和胴体性状差异,发现AA基因型与GG基因型猪肉质性状的4个值均差异显著,猪肌肉系水力、pH、大理石纹AA基因型显著高于GG、AG基因型;剪切力AA基因型显著低于AG(P<0.05)、GG(P<0.01)基因型;而胴体性状中三点背膘厚除活体C点背膘厚AA基因型高于AG基因型外,AA基因型均低于GG、AG基因型。结果表明,猪钙蛋白酶Ⅱ的AA基因型对于肉质和胴体性状而言是有益基因型,同时也说明CalpainⅡ基因参与了调控肌原纤维蛋白的降解,与促进动物骨骼肌生长、提高瘦肉率、改善肉嫩度等方面有一定相关。     

钙蛋白酶系统介导的骨骼肌蛋白降解

[摘要]钙蛋白酶系统由多种Ca2+激活的钙蛋白酶和钙蛋白酶抑制蛋白组成。钙蛋白酶系统介导的肌原纤维分解是骨骼肌蛋白降解的主要限速步骤,在维持肌肉生理稳态和肌肉完整性方面发挥了重要的作用。本文综述了钙蛋白酶系统的蛋白结构,激活和调控机制,以及日粮营养对钙蛋白酶系统的影响,以期为相关研究提供帮助。 [关键词]钙蛋白酶|蛋白降解|肌原纤维|骨骼肌|营养调控     

Preparation of Cathepsin L1 Monoclonal Antibody Against Fasclala gigantica and Establishment of Double Antibody Sandwich ELISA

【Objective】 This study was intend to obtain cathepsin L1(rFgCat L1) specific monoclonal antibody and construct the double antibody sandwich ELISA.【Method】 Five BALB/c mice were immunized with 1 mg/mL rFgCat L1 protein for four times.Mouse splenocytes were isolated and fused with SP2/0 cells to construct hybridoma cells.Strong positive hybridoma cell lines were screened, 1×10⁶ cells were injected intraperitoneally per mouse to prepare monoclonal antibodies.Antibody titer and antigenic epitope were detected using ELISA method, antibody subtype and specificity were identified using Western blotting method.The double antibody sandwich ELISA was constructed by combining the anti-rFgCat L1 polyclonal antibody, and its sensitivity and specificity were tested.The positive and negative critical value was screened by 20 negative sera with positive control, and the constructed double antibody sandwich ELISA was verified by 47 goat positive sera and 47 dairy cow positive sera.【Result】 After immunization, the antibody titers in serum of 4 mice were all more than 10⁴.After isolated mouse with the highest immune response spleen cells were fused with SP2/0 cells total of 8 of them were positive cell lines were obtained after selective culture.5D5 and 7G6 were identified as strong positive strains with stable antibody secretion.After multiple subcloning screens and subcultures, the antibodies secreted in the cell supernatant were stable, with titers of 2⁹ and 2¹⁰ respectively, with ascites titers of 10⁷ and 10⁸.Western blotting and antibody subtype identification kits identified that the two antibodies were IgG1 type and the light chain was kappa type, both of which could specifically bind FgESP.According to the same antigen site was recognized by the two kinds of antibodies, the antigen titer of the two monoclonal antibodies were comparied, 7G6 was used as the coating antibody, and anti-rFgCat L1 was used as the enzyme-labeled secondary antibody.The optimized condition of method was that 7G6 was coated at a concentration of 2 μg/mL, the dilution concentration of anti-rFgCat L1 polyclonal antibody was 25 μg/mL, the dilution of Don-HRP-conjugated was 1∶4 000, 5% skimmed milk powder was selected as the blocking solution and the color development time was 25 min.The method was proved that could recognize the lowest antigen concentration of 0.625 μg/mL, also could specifically recognize antigen of Fasciola fasciatus.The constructed sandwich ELISA method was used for antigen detection of 47 dairy cow positive serum and 47 goat positive serum infective samples kept in the laboratory and the positive antigen rate were 72.3% and 78.7%, respectively.【Conclusion】 Anti-rFgCat L1 monoclonal antibody was successfully prepared and the double-sheet sandwich ELISA method for fascioliasis was constructed, which provided a good theoretical basis and material basis for the development of low-cost and rapid diagnostic kits.     

哈茨木霉天冬氨酸蛋白酶基因SA76的3＇RACE扩增和序列分析

由EST获得全长cDNA对于结构基因组学和功能基因组学都是至关重要的,cDNA末端快速扩增技术RACE是该领域中的重要研究方法.利用BD SMART RACE技术扩增编码分泌天冬氨酸蛋白酶SA76基因的3＇末端,将其与哈茨木霉cDNA文库中的SA76基因的EST序列进行序列拼接,获得2019bp的全长cDNA序列,其开放读码框长1593bp,5＇非编码区266bp,3＇非编码区201bp,编码530个氨基酸,有信号肽.哈茨木霉天冬氨酸蛋白酶基因与玉蜀黍赤霉、粗糙脉孢菌、球毛壳菌天冬氨酸蛋白酶基因的同源性分别为53%, 37%, 36%.利用BD SMART RACE技术首次从哈茨木霉中克隆天冬氨酸蛋白酶基因,为验证SA76基因的功能奠定基础,为进一步研究蛋白酶的作用机制及生物防治功能提供依据.