阿岗镇玉米双套马铃薯高产栽培技术

根据玉米和马铃薯的生长特性,把玉米和马铃薯先进实用的栽培技术进行技术组装集成套种,是提高复种指数,增加粮食产量比较有效的一项综合性技术措施。     

Development of Nucleic Acid Lateral Flow Immunoassay Detection Method for Foot-and-mouth Disease Virus

In this article, through the combination of nucleic acid probes and immune chromatography, a simple, sensitive and specific detection system——nucleic acid lateral flow immunoassay (NALFIA) for amplifing foot-and-mouth disease virus (FMDV) 3D RT-PCR products was established.An ultrasensitive nucleic acid biosensor (NAB) based on streptavidin-labeled gold nanoparticles dual labels and lateral flow strip biosensor (LFSB) were used in this system.The biotinylated goat anti-rabbit IgG was marked to the NC membrane as the alleged strip and the anti-digoxin antibody was labeled to the NC membrane to capture the digoxin probe.After assemblying gold-labeled strip and detecting RT-PCR products, the detection limit of NALFIA was 0.3×10^-3 to 3×10^-3 μg/μL.The NALFIA was compared with agar gel electrophoresis analysis, the results showed that the sensitivity of NALFIA was higher than agar gel electrophoresis.There was an excellent agreement between the two methods.NALFIA was a method with high sensitive, low cost and short time.In conclusion, this method provided a good alternative to detect FMDV.     

坝堤防渗加固处理中套井回填施工技术

坝堤渗漏是水利工程中较为普遍的工程质量问题,为了消除水利工程的潜在安全威胁,通常对坝堤进行防渗加固处理,其中套井回填技术是较为有效的防渗加固技术,其具有增大周围土层的密实度和坝堤防渗性能等特点。基于此,主要阐述套井回填的工作原理、适用范围、工艺流程,同时探讨套井回填技术对坝堤防渗加固情况的分析及技术要求。     

苹果树夏季管理技术

6月份是果树管理的关键季节,果实套袋基本结束,做好以下工作至关重要。一、继续套袋6月上旬为套袋适宜期,没有套完的可延长至中旬,最晚不能超过6月20日。套完塑膜袋10-15d后再套纸袋,生产的果实个大、色泽好,高档果率在90%以上,可显著提高经济效益。     

CALIS标准下如何利用套录数据进行西文文献的编目

文章简要介绍了国内西文图书套录数据的两大数据源LOC和CALIS的基本情况,并详细分析了利用套录数据进行西文编目时应修改和完善的各个环节,进而严格控制编目质量,实现数据的规范化。     

半地下带保温夹套发酵罐的应用

通过对半地下带保温夹套的发酵罐与普通发酵罐24 h的温度变化和6 d的产气量的对比试验分析,结果表明:采用半地下带保温夹套发酵罐发酵,24 h的温度变化缓慢,受外界温度变化影响小,高温持续的时间较长,利于微生物发酵;产气总量多,持续时间长。     

农机配件不同去污方法各有招儿

保持农机配件的清洁对于农业机械的正常运转是很有好处的,但是不同的污垢处理起来确实很困难,下面就介绍几种清除农机污垢的方法,供大家参考。水垢清除方法1.除水垢。清除铁铸气缸等处水垢时,可将浓度为8%~10%的盐酸及相当于盐酸量3%的若丁缓蚀剂溶液加热至     

名企名品

<正>东方红LX1204型轮式拖拉机中国一拖集团有限公司地址:河南省洛阳市涧西区建设路154号电话:400-6591-899产品介绍LR6B5-23型发动机,(12+4)变速箱,啮合套换挡,外形尺寸为5 040 mm伊2 255mm伊2 870 mm,最小使用质量为4 350 kg,牵引力不小于40.5kN,提升力不小于28 kN,速度范围为2.19耀29.63 km/h,四轮驱动,液压转向,静液压制动系统。     

名企名品

<正>东方红LX1204型轮式拖拉机产品介绍LR6B5-23型发动机,(12+4)变速箱,啮合套换挡,外形尺寸为5 040 mm伊2 255mm伊2 870 mm,最小使用质量为4 350 kg,牵引力不小于40.5k N,提升力不小于28 k N,速度范围为2.19耀29.63 km/h,四轮驱动,液压转向,静液压制动系统。     

Establishment of a Duplex Real-time RT-PCR Assay for the Differential Detection of Nipha Virus and Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus

To establish a rapid,sensitive and specific assay for the differential detection of Nipah virus (NiV) and highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV),a duplex Real-time RT-PCR was developed with specific primers and probes targeting to the special sequences of NiV M gene and HP-PRRSV nsp2 gene by optimization of reaction conditions.The performance of the assay was linear ranging from 4.6×10¹ to 4.6×10⁷ copies/μL for RNA standard control of NiV M (NiV-M-RNA) and from 4.1×10¹ to 4.1×10⁸ copies/μL for RNA standard control of HP-PRRSV nsp2 (HP-PRRSV-nsp2-RNA),and detection limits of the assay was 46 copies for the NiV-M-RNA and 4.1 copies for the HP-PRRSV-nsp2-RNA,respectively.The coefficients of variation (CVs) of both inter-assay and intra-assay repeatability were less than 2.0%,showing good repeatability.The assay was able to specifically detect NiV and HP-PRRSV simultaneously without cross-reaction with classical swine fever virus (CSFV),porcine epidemic diarrhea virus (PEDV),swine influenza virus (SIV),porcine parvovirus (PPV),pseudorabies virus (PRV) and porcine circovirus type 2 (PCV2).Of the 236 samples from pigs for both NiV and HP-PRRSV detection by the established assay,all the samples were negative for NiV,8 samples were HP-PRRSV positive.In conclusion,this assay offers a useful approach for the differential detection of NiV and HP-PRRSV in clinical specimens from the pigs.