三七种子的脱水敏感性与膜质过氧化作用

采用常规生理生化方法对脱水处理后三七种子的活力、发芽率、相对电导率、丙二醛含量、抗氧化系统酶活性进行动态测定,研究其种子脱水敏感性及其影响机制。结果表明,三七种子的临界含水量为20%,随着脱水程度的加深,三七种子的活力、发芽率呈下降趋势,相对电导率和MDA含量逐渐增加,而抗氧化系统酶的活性总体呈先增后降的趋势。膜质的过氧化作用和抗氧化系统酶活性的降低是导致三七种子脱水敏感的主要原因。     

威灵仙酒炙提取工艺研究

以威灵仙根部为原料,采用黄酒用量、干燥条件、提取时间三因素,三水平的L9（34）正交试验设计,以水煎物干膏提取率、薄层层析检测威灵仙中的三萜皂苷为主要考核指标。结果表明：黄酒用量对威灵仙总皂苷提取率有极显著影响,烘干条件有显著影响,提取时间对试验结果影响不显著,表明影响因素最大的是黄酒用量,其次是干燥条件。     

田七黑斑病的调查

靖西县是我区田七生产的主要基地,全县种植面积达二万多亩,占全区种植总面积的90%以上,该县田七生产历史悠久.其病虫发生也较严重,为了解田七黑斑病的发生情况,1985～1986年,我们在靖西县对此病作了调查,其调查结果总结于后.     

三七炭疽病的发生及防治

三七炭疽病的发生及防治三七（又名田七）炭疽病（ＣｏｌｌｅｔｏｔｒｉｃｈｕｍＰａｎａｃｉｃｏｌａＶｙｅｄａｅｔＴａｋｉｍｏｔｏ）是三七的主要病害，在三七产区发生普遍，部分地区受害严重。影响产量及质量。植株发病时，叶上病斑呈灰绿色，有同心轮纹，后变褐色，...     

三七、黄芪、五味子、枸杞对小鼠SOD活性的影响

本实验研究了三七、黄芪、五味子、枸杞四味中药对小鼠血液以及脑、肝组织中超氧化物歧化酶（Superoxide Dismutose简称SOD）活性的影响。结果表明4味中药均能不同程度地使小鼠血液中以及脑、肝组织中SOD活性增高。     

Effects of Momordica grosvenori Saponins on Type 2 Diabetes Rats Induced by High-sucrose/High-fat Diet and Streptozotocin

The purpose of the experiment was to study the effect of Momordica grosvenori saponins on improving the effect of high-sucrose/high-fat diet combined with streptozotocin induced type 2 diabetes in rats.The type 2 diabetes rat model was established with a high-sucrose and high-fat diet combined with streptozotocin.Successfully modeled rats were given the total saponins of Momordica grosvenori (100,200,400 mg/kg) or metformin (268 mg/kg) by gavage,once a day for 4 weeks.Recording the "three more and one less"index (24 h internal urine output,water intake and feed intake,body weight);Detecting of biochemical indicators,analysis of fasting blood glucose (FBG),serum total cholesterol (TC),triglycerides (TG),low-density lipoprotein cholesterol (LDL-C),high-density lipoprotein cholesterol (HDL-C),insulin (INS),catalase (CAT),superoxide dismutase (SOD),malondialdehyde (MDA),alanine aminotransferase (AST),aspartate aminotransferase (ALT),blood urea nitrogen (BUN) and creatinine (Cr) levels.Hematoxylin-eosin staining (HE) was used to observe the pathological changes of the pancreas.Compared with the model group,the feed intake,water intake,urine output,blood indicators FBG,INS,MDA,TC,TG,BUN and Cr levels,AST and ALT activities were significantly or extremely significantly decreased in the total saponins of Momordica grosvenori each dose group and metformin group (P<0.05;P<0.01),while CAT,SOD activities and HDL-C level were significantly or extremely significantly increased (P<0.05;P<0.01).In pancreatic tissue,the number of pancreatic cells was increased significantly,and the pathological damage of the pancreas was significantly reduced. Momordica grosvenori saponins and metformin had obvious effects on improving glucose metabolism disorders,and at the same time could significantly reduce insulin resistance,enhance the body’s ability to resist oxidative stress and improve liver and kidney functions.     

药食兼用新型蔬菜——藤三七北方温室栽培技术

藤三七(Anredera cordifolia(Tenore)van Steen),又称洋落葵、藤子三七、川七。属于落葵科多年蔓生植物,原产于巴西,在我国很多地区均有种植,尤其在南方地区种植较多。藤三七的叶片、嫩梢可作为蔬菜食用,其味道鲜美,口感滑嫩,营养丰富。经测定藤三七叶片中除含有蛋白质、脂肪、     

人参茎叶皂苷对肉仔鸡生长性能和屠体品质的影响

人参(Ginseng)是我国传统的名贵中药,素有"百草药王"的美称。人参皂苷是人参的主要有效成分之一,对人和其它试验动物神经系统、心血管系统、内分泌系统、免疫系统等具有广泛的生物活性,已广泛地应用于临床实践[1～4]。     

人参总皂苷及其衍生物对小鼠淋巴细胞和NK细胞免疫活性的影响

为了提高人参总皂苷的生物学活性,对其进行了硫酸化修饰,并探讨总皂苷及其衍生物对小鼠脾T、B淋巴细胞增殖（MTT法）和NK细胞杀伤活性（乳酸脱氢酶释放法）的影响。结果表明,2种衍生物质量浓度在50～500 mg/L时可以促进小鼠脾T、B淋巴细胞的分化增殖和NK细胞对YAC-1的杀伤作用,质量浓度较高时促进作用明显优于总皂苷;衍生物质量浓度达到1 400 mg/L时,仍然可以显著促进T淋巴细胞的增殖。试验结果提示,人参皂苷的这种衍生物有可能成为一种毒副作用较小、免疫活性更强的药物。     

分子修饰前后人参皂苷体外抗马立克氏病毒鸡胚成纤维细胞模型的建立

用雏鸡将马立克氏病毒(MDV)血毒复壮,分离发病鸡淋巴细胞并接种于鸡胚成纤维细胞,观察其病变。获得适应鸡胚成纤维细胞(CEF)的MD强毒,通过电镜观察、琼脂扩散实验进一步鉴定病毒,并建立了MDV感染CEF细胞模型,为研究人参皂苷及其衍生物的体外抗病毒作用及其机制奠定基础。