信息技术用于汽车纵梁制造的探讨

文章以为汽车行业的健康发展提供指导和借鉴为目的,主要对汽车的纵梁平面图纸集成化制造中诸如参数提取、纵梁3D模型构建技术和Agent的网络集成技术的应用方法等进行了相关论述,以此为实现汽车行业的可持续发展而做出贡献。     

噬菌体Bp7尾丝蛋白gp37的原核表达与鉴定

为了研究噬菌体尾丝蛋白gp37在识别宿主菌大肠埃希菌K12过程中的作用,PCR扩增获得gp37基因C端1 161bp序列,以EGFP为荧光标签,构建重组表达质粒pET-28a-EGFP-gp37,在大肠埃希菌BL21(DE3)中IPTG诱导表达重组蛋白EGFP-gp37,亲和层析纯化蛋白。SDS-PAGE及Western blot检测结果表明,重组蛋白EGFP-gp37在大肠埃希菌BL21(DE3)为可溶性表达,表达量占菌体总蛋白量的23%。EGFP-gp37融合蛋白与大肠埃希菌K12相互作用,激光共聚焦显微镜检测结果显示,尾丝蛋白gp37能够吸附到宿主菌K12表面,表明尾丝蛋白gp37参与宿主菌受体的识别和吸附过程。     

国内外致泻大肠埃希氏菌O157:H7的检测标准比较及建议

致泻大肠埃希氏菌O157:H7（STEC O157:H7）是大肠埃希氏菌中致病性最严重的一种食源性致病菌,主要存在于牛肉、牛奶、水果及其制品中,对身体健康造成很大危害,甚至引发死亡。食品中STEC O157:H7检测尤为重要。本文对国内外STEC O157:H7的检测标准进行比较,提出我国标准在样品前处理、快速筛选方法的应用等方面需要加强,以便为该菌快速准确检测提供帮助,实现与国际标准化体系建设接轨,满足实验室检测需要。     

Measurement and Analysis of Prestressed Concrete Flat Beam- Waffle Slab System During Prestressing

During the pre - stressing of the unbonded prestressed concrete wide flat beam - waffle slab system, the displacements and strains of slab and the prestressed concrete beams were site - measured and the results were analyzed. The prestressing construction was simulated by finite element program. The prestressing efficiency of the unbonded prestressed concrete wide flat beam - waffle slab system was discussed. It indicates that the prestressing efficiency was affected by the in -plane stiffness of slab.     

A Study on the Reduction of Hexavalent Chromium Ion in the Process of Sintering and Ironmaking

The basic principle of using chromium leach residue as melting flux of blast furnace is introduced in this paper.The reduction thermodynamics of Na 2CrO 4 and chromium oxides in the process of sintering and ironmaking is studied,and the simulating experiments in the laboratory and industrial experiments in 600 m 3 blase furnace are carried out.The results of thermodynamical study and experimental study show that hexavalent chromium ion in the burden can be well eliminated in the process of sintering and ironmaking.     

A Receiving Surface of Perturbative Photon Fluxes in Interaction Between Gravitational Waves and EM Fields

According to Electrodynamics in curved spacetime, the interaction between gravitational waves (GW) and electromagnetic (EM) fields will produce perturbative electromagnetic fields. In a special EM system, namely, a Gaussian beam (Gb) propagating through a static magnetic field, the photon flux along the direction of the electric field of the Gb will be only a pure perturbative photon flux,when a GW propagates along the symmetric axis of a Gaussian beam. Detailed alanysis and calculation of the photon flux along several typical surfaces are performed, and a possible optimum plane which can receive perturbative photons up to 10~4s~(-1) is proposed. It is possible to provide an important method to detection of GW.     

Experimental Study on the Extended Length of Negative Reinforcement Cut-in Phases in Continuous Beams

In this paper through the static loading tests of 6 inflexion zones and 2 cantilever zones in full-size constrained R/C beams, the stress performance in rebars cut-in-phases and subjected to negative moment is studied. The stress properties of extended length of rebars with different shear-span ratiosare analysed on basis of the full measurement of strain distributions combined with the investigation on state and regularity of the diagonal cracks development and series of new knowledge are obtained. It is found that current Concrete Design Code provisions on the extended length are inadequate for the high shear-span ratio and superfluous for the low shear-span ratio. The theoretical and experimental foundations are provided for determining proper value of extended length for rebars cut-in-phases.     

Fire resistance performance on restrained high strength Q460 steel beams

为了研究约束高强度Q460钢梁的抗火性能,在已有约束钢梁分析理论的基础上,引入残余应力,提出了约束钢梁的抗火性能分析方法,并采用普通强度约束钢梁试验数据对分析方法进行了验证。考虑高强度Q460钢材高温下力学性能参数,利用所提出的方法分析了约束高强度Q460钢梁的抗火性能,并与普通强度Q345钢梁进行了对比。对影响约束高强度Q460钢梁的抗火性能参数进行了分析,包括荷载比、残余应力、轴向约束刚度、转动约束刚度和受火方式等。研究表明：所提出的分析方法准确可靠,高强度Q460钢梁抗火性能与普通强度钢梁具有较大的区别,高强度Q460约束钢梁的抗火性能明显优于普通强度约束钢梁。荷载比、轴向约束刚度、转动约束刚度、受火方式对高强度Q460约束钢梁有较大影响。