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Big bluestem (Andropogon gerardii Vitman) and little bluestem [Schizachyrium scoparium (Michaux) Nash.] are native to the North America and are important forage grasses and ornamental grasses. Both grasses are proposed as ideal biomass producers for cellulosic ethanol production. To apply genetic transformation, which is an important tool for incorporating desirable agronomic traits into plants to both species, however requires an efficient and reproducible regeneration protocol. We used mature caryopses from big and little bluestem as explants and tested the effect of various combinations of 2, 4-dichlorophenoxyacetic acid (2, 4-D) (1, 2, 3, 4 or 5 mg l−1) and kinetin (KT) (0, 0.1 or 0.2 mg l−1) on embryogenic callus induction with LS as the basal medium. The highest percentage of embryogenic calli induction occurred on medium containing 2, 4-D alone at 2 mg l−1 for ‘Bison’ and on medium containing 4 mg l−1 2, 4-D alone for ‘Bonilla’ big bluestem. For little bluestem, the highest percentage of embryogenic callus induction occurred on medium containing 3 mg l−1 2, 4-D plus 0.1 mg l−1 kinetin, suggesting that addition of KT is beneficial. Shoot regeneration took place on LS basal medium without any plant growth regulator for both species, although the addition of KT increased both regeneration frequency and the number of shoots produced per callus. Rooting of shoots reaching about 2 cm long occurred readily with or without α-naphthaleneacetic acid (NAA). Rooted plantlets were all successfully established in the soil. 相似文献
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J. Kocielniak 《Journal of Agronomy and Crop Science》1993,171(2):73-81
Night chilling (5 °C) subsequently lowered photosynthetic intensity in the leaves of maize seedlings at 20 °C through an increase in leaf diffusive resistance brought on by lower tissue water content in morning hours. A more significant increase in leaf diffusion resistance was observed when soil temperature was lowered than in the case of lower air temperature.
The unfavorable effect of soil and air cooling temperature on photosynthesis was limited by air saturated with water vapour. However, as a result of lowering the night temperature from 5 °C to 1 °C, the efficiency of the protective influence of higher atmospheric humidity was decreased. This demonstrates that the participation of factors unrelated to plant water status in inhibiting photosynthesis increases with lower night temperatures.
An additional reason for inhibited photosynthesis following cool nights was a decrease in chlorophyll accumulation, below 50 μg per 1 cm2 of leaf area. 相似文献
The unfavorable effect of soil and air cooling temperature on photosynthesis was limited by air saturated with water vapour. However, as a result of lowering the night temperature from 5 °C to 1 °C, the efficiency of the protective influence of higher atmospheric humidity was decreased. This demonstrates that the participation of factors unrelated to plant water status in inhibiting photosynthesis increases with lower night temperatures.
An additional reason for inhibited photosynthesis following cool nights was a decrease in chlorophyll accumulation, below 50 μg per 1 cm
46.
J. Cabaret H. M. Baudet J. Devos J. Hubert J. Cortet C. Sauv 《Veterinary parasitology》1995,60(3-4):331-337
Multispecific resistance to benzimidazoles was studied in three selected farms. These farms had bred dairy goats for more than 15 years. The helminths were introduced with the goats at the establishment of the farms which afterwards remained isolated. Nematode resistance could then be related to their own management practices. Faecal egg count tests and egg hatch assays were performed to assess intensity of resistance. The generic (infective larvae in faecal cultures) and specific richness (adult worms) were assessed. The resistant species were Trichostrongylus colubriformis, Teladorsagia circumcincta, Haemonchus contortus and Oesophagostomum venulosum. Faecal egg count reduction tests and egg-hatch assays did not match exactly. Faecal larval counts after treatments gave a distorted picture of multispecific resistance: Haemonchus and Oesophagostomum were very largely over represented. The number of species found in the three farms was relatively low compared with other reports in goat farms of the area. This reduction of diversity might also be due in part to characteristics of breeding management and history (use of permanent pasture and introduction of goats at the establishment of farm). 相似文献
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Abstract – Foraging juvenile fish with relatively high food demands are usually vulnerable to various aquatic and avian predators. To compromise between foraging and antipredator activity, they need exact and reliable information about current predation risk. Among direct predator-induced cues, visual and olfactory signals are considered to be most important. Food intake rates and prey-size selectivity of laboratory-reared, naive young-of-the-year (YOY) perch, Perca fluviatilis , were studied in experiments with Daphnia magna of two size classes: 2.8 and 1.3 mm as prey and northern pike, Esox lucius , as predator. Neither total intake rate nor prey-size selectivity was modified by predator kairomones alone (water from an aquarium with a pike was pumped into the test aquaria) under daylight conditions. Visual presentation of pike reduced total food intake by perch. This effect was significantly more pronounced (synergistic) when visual and olfactory cues were presented simultaneously to foraging perch. Moreover, the combination of cues caused a significant shift in prey-size selection, expressed as a reduced proportion of large prey in the diet. Our observations demonstrate that predator-induced olfactory cues alone are less important modifiers of the feeding behaviour of naive YOY perch than visual cues under daylight conditions. However, pike odour acts as a modulatory stimulus enhancing the effects of visual cues, which trigger an innate response in perch. 相似文献
48.
中国结缕草属(Zoysia spp.)植物抗寒性评价 总被引:30,自引:10,他引:20
在广泛收集结缕草属(ZoysiaWild.)种质资源基础上,按照形态类型和地理分布,选取40份种质,采用电导法(EL)对其抗寒性进行初步评价。以半致死温度(LT50)为评价指标,在参试材料中除大穗结缕草和来自台湾岛海边沙地的沟叶结缕草外,其它种质都较天堂-419(对照)抗寒;在结缕草属内,抗寒性具有明显的种间差异,耐寒性依次为日本结缕草>中华结缕草>沟叶结缕草>细叶结缕草>长花中华结缕草>大穗结缕草,其中细叶类型的沟叶结缕草和细叶结缕草抗寒性变异较大;日本结缕草和中华结缕草抗寒性变异与地理分布之间没有显著关系,但分布在海边的种类和种质抗寒性明显较低;结缕草属及其中华结缕草的抗寒性与叶长、叶宽以及叶背面被毛之间均存在显著的负相关关系,但这种关系不体现在日本结缕草上。实验结果还表明,EL方法是评价结缕草属抗寒性较为可靠的方法之一。 相似文献
49.
SMD残留检测的ELISA方法的建立和初步应用 总被引:12,自引:0,他引:12
用戊二醛法将SMD与载体蛋白BSA偶联 ,制备合成抗原SMD BSA作免疫原 ,同法合成包被抗原SMD OVA ,免疫健康家兔获得抗血清。分别用双向琼脂扩散试验和ELISA试验对抗血清进行定性定量测定 ,结果表明所获抗血清特异性针对SMD。用所制备的抗血清建立间接竞争ELISA方法。优化了ELISA的工作条件 ,方阵测定确定了包被抗原最佳浓度 (50 μg/mL) ,抗血清最佳稀释度 (1∶1 0 0 ) ,酶标抗体的最适工作稀释度 (1∶50 0 ) ,并建立了ELISA标准工作曲线。工作曲线表明在 1 0~ 2 0 0 0 μg/L浓度范围内呈良好的线性关系。该法检测底限为 63μg/L,低于国际规定残留限量 (1 0 0 μg/kg)和国内规定残留限量 (30 0 μg/kg)的要求。本法同时测定了回收率以及实际样品血清中的SMD的残留含量 相似文献
50.
A combined penetrometer is an appropriate tool to measure the soil cone resistance and the water‐content profile. As a relatively new technique, a combined capacitance‐penetrometer for the simultaneous measurement of cone index and soil water content was developed at the Department of Agricultural Engineering of Bonn University in 2002. The objective of this study was the evaluation of the effectiveness and applicability of the innovated penetrometer with a focus on three aspects: (1) A capacitance sensor with two electrode configurations was calibrated for silt loam, sandy loam, and sand. The calibration results show that both electrode configurations have sufficient water‐content sensitivity, but soil‐specific calibrations seem necessary. (2) Under laboratory conditions, the dynamic resolution and response of the capacitance‐penetrometer were validated, and its radius of influence was determined. (3) The field measurement results demonstrate that this measurement technique can be used to improve the interpretation quality of soil cone index data. 相似文献