圈养秦岭羚牛夏季昼间活动时间分配研究

为了对秦岭羚牛进行有效的迁地保护和揭示圈养条件下秦岭羚羊的夏季昼间的时间分配状况,更好地加强饲养管理,2006年7月,在陕西省珍稀野生动物抢救饲养研究中心内采用瞬时扫描取样法对13头圈养秦岭羚牛(Budorcas taxicolor bedfordi)的活动规律进行了观察。结果表明,夏季白昼羚牛的活动以休息为主,采食次之,同时,羚牛的饮水、排尿、排便量均较低,分别为每头每天1.92次、0.54次、0.92次。羚牛每天的活动节律与气温的变化相关。年龄对羚牛活动节律的影响主要表现在羚牛的活动节律和时间分配方面的差异。此外,从羚牛群体进行观察,同一群中的羚牛个体的活动具有不完全同步性。     

Pathogenesis-related gene expression in rice is correlated with developmentally controlled Xa21-mediated resistance against Xanthomonas oryzae pv. oryzae

Disease resistance mediated by the resistance gene Xa21 is developmentally controlled in rice. We examined the relationship between Pathogenesis Related (PR) defense gene expression and Xa21-mediated developmental disease resistance induced by Xanthomonas oryzae pv. oryzae (Xoo). OsPR1a, OsPR1b, and OsPR1c genes were cloned and their induction was analyzed, in addition to the OsPR10a gene, at the juvenile and adult stages in response to a wildtype Xoo strain that induces a resistance response (incompatible interaction) and an isogenic mutant Xoo strain that does not (compatible interaction). We found that the adult stage leaves are more competent to express these OsPR1 genes and that the Xa21 locus is required for the highest levels of induction.     

Evaluation of turnip-rooted parsley (Petroselinum crispum ssp. tuberosum) for root and foliage production under a warm,Mediterranean climate

Two experiments were undertaken in southern Greece during 2000–2002 in which turnip-rooted parsley was tested for its suitability for cultivation in comparison with locally grown curly-leafed and plain-leafed forms. Plants were harvested when the foliage was acceptable for marketing, rather than on completion of the vegetative cycle, hence root size was relatively small. In year 1, the foliage development of plain-leafed parsley was significantly higher than that of the other two forms, irrespective of sowing date. In year 2, both plain-leafed and curly-leafed parsley produced more foliage than turnip-rooted parsley in the early sowing (November), but not in subsequent sowings (December and February). During both years, root development was higher in turnip-rooted parsley than in curly-leafed parsley, although in the November and February sowings of year 1 the root weight of both plain-leafed and turnip-rooted forms were similar. In all three types of parsley, foliage yield fell off significantly in the spring sowing (February), and in year 1 there was a considerable decrease in root production as well. In most cultivars, foliage weight was higher when plants were grown from transplanted seedlings rather than by direct sowing, whereas the effect of sowing method on root weight was sowing date dependent. Cultivar differences in the foliage weight of direct sown and transplanted plants were reflected by differences in plant height and, in some cases, by leaf number. It is concluded that although overall turnip-rooted parsley produced rather less foliage than that plain-leafed parsley, this was compensated for by the satisfactory production of roots and therefore turnip-rooted cultivars may constitute a suitable crop for introduction to the Mediterranean region.     

氧氟沙星在肉鸡体内残留的研究

建立了氧氟沙星在肉鸡组织内残留的高效液相色谱分析法。采用荧光检测器，激发波长278nm，发射波长445nm。色谱柱为Hypersil BDS—C18，柱温40℃；以磷酸（三乙胺调pH至2．5）-乙腈（86：14）为流动相，流速1．0mL／min；进样量为20μL。氧氟沙星在肌肉、肝脏和肾脏中的平均回收率分别为96．3％、87．5％和76．4％，检测限为10ng／g，在10～1000ng／mL浓度范围内，氧氟沙星的峰面积与其浓度呈良好的线性相关，达到了氧氟沙星残留分析的要求。利用建立的方法对氧氟沙星在肉鸡体内的残留消除规律进行了研究，以1L水添加500mg的剂量，对120只肉鸡连续给药5d，对不同停药时间所取肌肉、肝脏和肾脏样品中的药物残留进行分析。结果表明，氧氟沙星在肉仔鸡组织中的残留消除速度较为缓慢，至休药23d时，仍可在肝脏和肾脏分别检出39和35μg/kg的氧氟沙星残留。在本实验的条件下，建议氧氟沙星临床休药期以25d为宜。     

55年来黄土高原边缘地区耕地动态变化的多尺度分析

本文对地处干旱、半干旱黄土高原生态脆弱地带的大同市耕地现状和1949—2003年间耕地总量变化进行分析,同时采用Morlet小波变换方法,对大同市耕地面积变化进行了多时间尺度和相应的周期变化特征分析,得出55年来大同市耕地面积变化具有2—3年和6年左右的年际尺度周期变化,并预测未来大同市耕地面积变化趋势;运用主成分分析方法分析得出耕地动态变化的驱动机制为人口因素、农业现代化水平、经济发展水平三个方面,所得结论有利于在科学的指导下采取措施稳定耕地面积,为干旱半干旱地区耕地的可持续利用提供科学依据,对相关省市农业可持续发展具有重要意义。     

农牧交错带不同封育时间对植物特征值及多样性的影响

为研究我国北方农牧交错带不同封育时间对干旱、半干旱草原植物特征值及多样性的影响,同时探索农牧交错带草原封育周期,本研究选取位于我国农牧交错带的宁夏盐池人工封育草原为研究对象,采用样方调查法,于每年7-8月份(植物生长季节)对封育区植被生长状况进行调查,调查内容包括:植物名称、植物种数、株数、盖度、高度、地上生物量(鲜重)等。运用生态学、地统计学原理,结合Biodiversity Pro、Microcal Origin等生态学统计软件对外业数据进行处理、分析,结果表明:封育2-3年后植物群落优势种优势地位得到巩固,而一些伴生种则在竞争中消失;封育区植物特征值在封育2-3年后达到最大,如地上生物量(鲜重)在封育3年达到最大值,为6907.75kg/hm2,此后呈下降趋势;封育区主要植物重要值及植物物种多样性均在封育2-3年后出现最大值,此后也不同程度地呈下降趋势。     

Relating plant and pathogen development to optimise fungicide control of phoma stem canker (<Emphasis Type="Italic">Leptosphaeria maculans</Emphasis>) on winter oilseed rape (<Emphasis Type="Italic">Brassica</Emphasis><Emphasis Type="Italic">napus</Emphasis>)

In winter oilseed rape experiments at Rothamsted in 2000/01 to 2002/03 growing seasons, the severity of phoma stem canker epidemics in summer depended on the timing of phoma leaf spot epidemics in the previous autumn, and hence on the timing of Leptosphaeria maculans ascospore release. The first major release of L. maculans ascospores was earlier in 2000 (26 September) and 2001 (18 September) than in 2002 (21 October). Consequently, the autumn phoma leaf spot epidemic was also earlier in 2000 and 2001 than in 2002. The resulting stem canker epidemics were severe by harvest (July) in 2001 and 2002 but not in 2003. No correlation was found between the severity or duration of phoma leaf spotting (lesion days or lesion °C-days) and the subsequent severity of phoma stem canker epidemics. Rates of leaf production and loss were similar in the three growing seasons. Out of ca. 25 leaves produced on plants during each season, leaf numbers 10–14 generally remained on plants for the longest. Treatment with flusilazole + carbendazim in autumn decreased the severity of phoma leaf spotting for several weeks after treatment, decreased the severity of stem canker the following summer and increased yield significantly in 2001 and 2002 but not in 2003. The most effective timings for flusilazole + carbendazim application were when leaves 7–11 were present on most plants and at least 10% of plants were affected by phoma leaf spot. Two half-dose applications of fungicide reduced phoma stem canker and increased yield more than a single full dose application when phoma leaf spot epidemics were early (<800 °C-days after sowing).     

沈阳市休闲农业市场需要分析

沈阳市休闲农业虽处于初期发展阶段，但广大农村拥有丰富的旅游资源，市场空间和需求潜力巨大。介绍沈阳市休闲农业的发展现状、需求主体及发展趋势，分析其发展中存在的主要问题，并提出切实可行的发展建议。     

Simultaneous measurement of soil water and soil hardness using a modified time domain reflectometry probe and a conventional cone penetrometer

Soil strength and water content are important indices for assessing soil resistance to root growth and soil compaction both of which affect other soil properties. Therefore, simultaneous measurement of soil penetration resistance (PR) and soil water content can aid agricultural land management. We measured PR with a conventional cone penetrometer, followed immediately by determining water content using a modified TDR probe inserted into the penetrometer hole. From the results of a field feasibility test, soil water content was measured satisfactorily and correlated well with data obtained by the gravimetric method, except for those data from near the surface owing to soil disturbance when the cone penetrometer was extracted after the PR measurements. Field results demonstrate that PR and soil water content have three‐dimensional variability, with a markedly different distribution pattern between cultivated and subsoil layers at the field scale. Overall, the variability in the PR and soil water data is similar to that reported in previous studies. We conclude that our method produces results helpful to field management of soil and water because it is based on a simple and easy technique for the simultaneous measurement of soil water content and PR.     

新疆国际旅游客源市场研究

新疆具有明显的旅游资源优势,是中国很有吸引力和巨大潜力的旅游地区。特别是近年来,国际旅游发展较快。因此,对其国际旅游客源市场结构进行研究,有利于进行明确的国际客源市场定位,制定切实可行的客源市场开拓策略,进一步加快国际旅游的发展。本文在分析大量数据的基础上,利用了年际变化强度指数和地理集中指数对新疆国际旅游客源市场的时间结构、空间分布结构进行了分析,然后利用SPSS统计软件建立了新疆国际旅游客源市场的线性回归预测模型,并提出了今后新疆国际旅游客源市场的开拓对策,为新疆国际旅游业的发展提出了一些设想。