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101.
102.
以永平县林业矢量数据为例,利用91卫图助手软件和ArcGIS软件,通过北京54坐标系矢量数据在各坐标系下叠加、坐标系转换参数的计算、矢量数据坐标系的转换等步骤,阐述实现矢量数据坐标系WGS84、北京54、西安80和国家2000坐标系相互转换的方法,以实现各类自然资源矢量数据的共享。 相似文献
103.
本研究通过采用氮的K边X光吸收近边结构(XANES)技术来了解森林土壤有机氮,以此深入研究土壤中有机氮种类与其转化的定量关系。土壤样品采自台湾中部的云杉、铁杉林与草地。结果表明,氮的 XANES 可以揭示样品中不同的氮的种类。在土壤腐植质、可溶性氮及本体土壤中胺态及吡咯氮占了主要的比率。然而不同处理及植被下的土壤样品氮的种类分布是不同的。云杉与铁杉土壤可溶性有机氮在402.3 eV 能量处有显著的差异。在A层土壤中,吡啶类氮含量要显著高于O层土壤,说明氮在不同土层中的转化率存在极大的差异,这种变化对于氮在森林土壤中的循环将起重要作用。图3表1参8。 相似文献
104.
105.
不同因素对农杆菌介导的杉木转化效率的影响 总被引:1,自引:0,他引:1
以杉木试管苗靠近茎尖的茎段为受体材料,研究6个影响杉木遗传转化效果的因素,初步确定杉木茎段较为合适的转化体系为:预培养1~3 d,OD600 nm为0.1~0.4的菌液浸染10~15 min,共培养3~5 d,共培养基附加乙酰丁香酮(AS)80 μmol·L-1,共培养后延迟3 d筛选.在初次筛选培养基上共得到186个卡那霉素(Km)抗性芽,在二次筛选培养基上获得39个Km抗性芽.PCR检测有2个Km抗性芽呈稳定阳性,初步证明外源天麻抗真菌蛋白(GAFP)基因已整合到这2个Km抗性芽的基因组DNA中. 相似文献
106.
厦门城市森林马尾松宽带更新改造技术研究 总被引:1,自引:0,他引:1
厦门城市森林中马尾松劣质林分比例大,采用不同改造方式试验表明:宽带采伐更新改造效果较好,阔叶树种造林成活率高,幼林生长比较快,适宜阳性、强阳性和中性偏阳树种。且宽带更新改造技术较简单,可操作性强,便于推广应用。 相似文献
107.
Katsuaki Ishii 《Journal of Forest Research》2002,7(2):99-104
Hinoki cypress (Chamaecyparis obtusa) is one of the most important timber resource forest trees in Japan. Because seed production from a seed orchard of hinoki
cypress is not constant every year, micropropagation from a limited amount of material is useful. Up to now, the conventional
tissue culture method using solid medium has been used. Here a new method using liquid culture in tubes rotated vertically
is described. Shoot primordium of hinoki cypress was inoculated in Campbell and Durzan’s (CD) liquid medium containing different
cytokinins (6-benzylaminopurine (BAP), Zeatin, thidiazurone (TDZ)), and the container tubes were rotated vertically around
the axis at 2 times / min. Culture room temperature was 25°C and light condition was 16 h photoperiod per day of fluorescent
lamps. Zeatin at 1μM concentration was the best for maintaining the shoot primordium production and TDZ induced callus on the surface of the
shoot primordia. After shoot primordium multiplication in the liquid culture, they were transplanted to agar medium for shoot
elongation. A high concentration of agar (up to 16 g/L) or AVF (anti vitrification factor from Dr. Nairn, 1995) was effective
to prevent vitrification of the shoots. Transformation of shoot primordium was done using particle bombardment with vectors
containingβ-glucuronidase (GUS) gene or herbicide resistance gene (bar). Positive result for transient transformation was observed with the histo-chemical study for transformation with GUS. Integration
of a useful herbicidebar gene into the shoot primordium culture system was also tried and stably transformed plants were obtained. This is the first
report of stable transformation of Japanese conifer using practically useful gene.
The generous supply of AVF-B from Dr. B.J. Nairn, Tasman Forestry, NZ is also appreciated. 相似文献
108.
One-day rate measurements for estimating net nitrification potential in humid forest soils 总被引:2,自引:0,他引:2
Donald S. Ross Guinevere Fredriksen Austin E. Jamison Beverley C. Wemple Scott W. Bailey James B. Shanley Gregory B. Lawrence 《Forest Ecology and Management》2006,230(1-3):91-95
Measurements of net nitrification rates in forest soils have usually been performed by extended sample incubation (2–8 weeks), either in the field or in the lab. Because of disturbance effects, these measurements are only estimates of nitrification potential and shorter incubations may suffice. In three separate studies of northeastern USA forest soil surface horizons, we found that laboratory nitrification rates measured over 1 day related well to those measured over 4 weeks. Soil samples of Oa or A horizons were mixed by hand and the initial extraction of subsamples, using 2 mol L−1 KCl, occurred in the field as soon as feasible after sampling. Soils were kept near field temperature and subsampled again the following day in the laboratory. Rates measured by this method were about three times higher than the 4-week rates. Variability in measured rates was similar over either incubation period. Because NO3− concentrations were usually quite low in the field, average rates from 10 research watersheds could be estimated with only a single, 1-day extraction. Methodological studies showed that the concentration of NH4+ increased slowly during contact time with the KCl extractant and, thus, this contact time should be kept similar during the procedure. This method allows a large number of samples to be rapidly assessed. 相似文献
109.
基于1998~2018年黑龙江省国有林业产业数据,从发展规模、产业结构、投入产出效益、专业化水平等4个方面对黑龙江省国有林业产业发展现状进行总体概述和分析。借助产业贡献度指标测算国有林业产业对黑龙江省经济发展的贡献水平,并基于三次产业构成进一步对国有林业产业贡献度进行了结构性动态评价。研究得出:黑龙江省国有林业产业对地区经济发展贡献呈现稳步上升的特征,第一产业表现突出,第二、三产业呈小幅下降、小幅上升的反向变动特征。基于结果分析,从产业自身发展和持续推进多种经营产业转型两个方面提出方向性建议。 相似文献
110.