Protein and energy concentrations of meat meal and meat and bone meal fed to pigs based on in vitro assays

The objective of this study was to develop equations for estimating ileal digestible crude protein (CP) and metabolizable energy (ME) contents of meat meal (MM) and meat and bone meal (MBM) as feed ingredients for pigs based on in vitro assays. Test ingredients were 4 sources of MM and 3 sources of MBM. Ash and CP contents of the ingredients ranged from 3.8% to 33.1% and 46.8% to 82.9% (as-is basis), respectively. In vitro ileal disappearance (IVID) of CP was determined and ileal digestible CP content was calculated by multiplying CP content by IVID of CP. In vitro total tract disappearance (IVTTD) of dry matter (DM) was determined and ME was calculated using gross energy, CP contents, and IVTTD of DM. The IVID of CP and IVTTD of DM ranged from 77.2% to 88.7% and from 82.7% to 92.4%, respectively. Calculated ileal digestible CP and ME contents ranged from 37.8% to 73.5% DM and 2,405 to 3,905 kcal/kg DM, respectively. Ash contents were negatively correlated (P < 0.001) with CP (r = −0.99), in vitro ileal digestible CP (r = −0.97), gross energy (r = −1.00), in vitro digestible energy (r = −0.97), and adjusted ME (r = −0.97). The most fitting equations for ileal digestible CP and adjusted ME were: ileal digestible CP (% DM) = 11.91 − 0.90 × Ash (% DM) + 0.74 × IVID of CP (%) (R² = 0.99) and adjusted ME (kcal/kg DM) = 130.85 − 50.90 × ash (% DM) + 47.06 × IVTTD of DM (%) (R² = 0.99). To validate the accuracy of the prediction equations for ME, mean bias and linear bias were determined using a regression analysis. Calculated ME values of MM and MBM were in a good agreement with data obtained from animal experiments based on a statistically insignificant bias in the models. In conclusion, ME concentrations of MM and MBM as swine feed ingredients can be calculated using ash concentration and in vitro disappearance of dry matter.     

Estimation of Runoff,Peak Discharge and Sediment Load at the Event Scale in a Medium‐Size Mediterranean Watershed Using the Annagnps Model

In this paper, the Annualized Agricultural Non‐Point Source (AnnAGNPS) model has been used to estimate runoff, peak discharge and sediment load at the event scale in a Mediterranean watershed. The study area is the Carapelle torrent, Southern Italy (area = 506 km²), where continuous rainfall, streamflow and sediment load data are available. Nineteen flood events have been registered in the period 2007–2009 and were used for the application of the model. The aim of the paper is to evaluate the predictive accuracy of the model at the event scale, in a medium‐size watershed, given the specific conditions of the semi‐arid environments. A sensitivity analysis has been carried out to assign the correct parameterization: the mean normalized output variation of the most meaningful input parameters pointed out the influence of the curve number on runoff, peak discharge and sediment load predictions (values greater than 1); the MN Manning's roughness coefficient and K, C and P factors of the universal soil loss equation showed a moderate influence on sediment load simulations (values between 0·5 and 1). The selection of the Soil Conservation Service synthetic storm types has been based on the observed storm events analysis to improve the peak discharge simulations. The model prediction has proved to be good for runoff (R² = 0·74, NSE = 0·75, W = 0·92) and peak discharge (R² = 0·85, NSE = 0·70, W = 0·94), and satisfactory for sediment yield (R² = 0·70, NSE = 0·63, W = 0·91). The relative error is lower for high events; this result is quite interesting in semi‐arid environments, where most of the annual sediment yield is concentrated in a few, severe events. Copyright © 2013 John Wiley & Sons, Ltd.     

Impact of Land Use and Land Cover Changes on Organic Carbon Stocks in Mediterranean Soils (1956–2007)

During the last few decades, land use changes have largely affected the global warming process through emissions of CO₂. However, C sequestration in terrestrial ecosystems could contribute to the decrease of atmospheric CO₂ rates. Although Mediterranean areas show a high potential for C sequestration, only a few studies have been carried out in these systems. In this study, we propose a methodology to assess the impact of land use and land cover change dynamics on soil organic C stocks at different depths. Soil C sequestration rates are provided for different land cover changes and soil types in Andalusia (southern Spain). Our research is based on the analysis of detailed soil databases containing data from 1357 soil profiles, the Soil Map of Andalusia and the Land Use and Land Cover Map of Andalusia. Land use and land cover changes between 1956 and 2007 implied soil organic C losses in all soil groups, resulting in a total loss of 16·8 Tg (approximately 0·33 Tg y⁻¹). Afforestation increased soil organic C mostly in the topsoil, and forest contributed to sequestration of 8·62 Mg ha⁻¹ of soil organic C (25·4 per cent). Deforestation processes implied important C losses, particularly in Cambisols, Luvisols and Vertisols. The information generated in this study will be a useful basis for designing management strategies for stabilizing the increasing atmospheric CO₂ concentrations by preservation of C stocks and C sequestration. Copyright © 2012 John Wiley & Sons, Ltd.     

基于低碳与成本约束的机电产品包装概念设计

机电产品的包装需要耗费大量的原木,对环境造成了极大的影响,也增加了机电产品的生产经营成本。文章从低碳和成本约束的角度,对机电产品包装概念设计的模型进行了分析和探讨。     

基于ANSYS的永磁电机转子冲片结构的优化设计

运用ANSYS的优化设计模块对永磁电机转子冲片隔磁桥附近的结构进行优化设计,采用APDL参数化设计通过拓扑形状优化功能确定冲片优化的初始区域,在此基础上运用尺寸优化功能确定最优的形状尺寸参数,最终结合工程化要求确定转子冲片的施工结构尺寸。电机最后通过最高转速试验表明文章运用的结构优化设计方法切实可行,满足工程化生产要求。     

6种灌木不同器官生物量及其碳、氮含量的分配特征

通过研究晋北地区草地黄刺玫、沙棘、荆条、酸枣、绣线菊和柠条等6种常见灌木的叶片、新生枝、老龄枝生物量及其碳、氮含量的分配特征,旨在为草地准确估算草地植物碳、氮循环提供依据。结果表明:黄刺玫的地上生物量最大,是酸枣的7倍。沙棘叶片生物量占地上生物量的比例最大;黄刺玫新生枝生物量占地上生物量的比例最大;柠条老龄枝生物量占地上生物量的比例最大。黄刺玫、沙棘和荆条的地上部分各器官生物量分配特征相似,由高到低表现为叶片、老龄枝、新生枝;绣线菊、酸枣和柠条的地上部分各器官生物量分配特征相似,由高到低表现为老龄枝、叶片、新生枝。6种灌木地上部分各器官碳含量差异不显著,氮含量分布由高到低为叶片、新生枝、老龄枝。     

放牧管理对西藏高寒沼泽植物群落结构和土壤碳氮的影响

对西藏当雄县3种不同放牧管理模式(冬季放牧、冬春季放牧和全年放牧)下的高寒沼泽的植物群落结构和土壤碳氮进行了调查。调查发现,3种放牧管理模式下的高寒沼泽植物群落总盖度差异不显著,而优势物种藏嵩草的高度和盖度差异显著;放牧管理对沼泽群落结构和物种多样性影响较大,随着放牧强度的增加,物种丰富度增加;放牧利用显著降低了高寒沼泽湿地群落优势物种藏嵩草的高度和盖度,其优势度也显著降低,使得高寒沼泽逐渐退化。冬季放牧、冬春季放牧和全年放牧沼泽地上生物量和地下生物量依次降低。同时,随着放牧强度的增加,高寒沼泽表层土壤有机碳氮的含量也显著降低,且高寒沼泽表层土壤碳氮与地上和地下生物量显著正相关。通过对比分析,冬季放牧是当前西藏高寒沼泽较好的放牧管理模式。     

Molecular cloning,purification and immunogenicity of recombinant Brucella abortus 544 malate dehydrogenase protein

The Brucella mdh gene was successfully cloned and expressed in E. coli. The purified recombinant malate dehydrogenase protein (rMDH) was reactive to Brucella-positive bovine serum in the early stage, but not reactive in the middle or late stage, and was reactive to Brucella-positive mouse serum in the late stage, but not in the early or middle stage of infection. In addition, rMDH did not react with Brucella-negative bovine or mouse sera. These results suggest that rMDH has the potential for use as a specific antigen in serological diagnosis for early detection of bovine brucellosis.     

Impact of variation in structure of condensed tannins from sainfoin (Onobrychis viciifolia) on in vitro ruminal methane production and fermentation characteristics

Our study investigated the effects of condensed tannins (CT) on rumen in vitro methane (CH₄) production and fermentation characteristics by incubating lucerne in buffered rumen fluid in combination with different CT extracts at 0 (control), 40, 80 and 120 g CT/kg of substrate DM. Condensed tannins were extracted from four sainfoin accessions: Rees ‘A’, CPI63763, Cotswold Common and CPI63767. Gas production (GP) was measured using a fully automated GP apparatus with CH₄ measured at distinct time points. Condensed tannins differed substantially in terms of polymer size and varied from 13 (Rees ‘A’) to 73 (CPI63767) mean degree of polymerization, but had relatively similar characteristics in terms of CT content, procyanidin: prodelphinidin (PC: PD) and cis:trans ratios. Compared to control, addition of CT from CPI63767 and CPI63763 at 80 and 120 g CT/kg of substrate DM reduced CH₄ by 43% and 65%, and by 23% and 57%, respectively, after 24‐h incubation. Similarly, CT from Rees ‘A’ and Cotswold Common reduced CH₄ by 26% and 46%, and by 28% and 46% respectively. Addition of increasing level of CT linearly reduced the maximum rates of GP and CH₄ production, and the estimated in vitro organic matter digestibility. There was a negative linear and quadratic (p < 0.01) relation between CT concentration and total volatile fatty acid (VFA) production. Inclusion of 80 and 120 g CT/kg of substrate DM reduced (p < 0.001) branched‐chain VFA production and acetate: propionate ratio and was lowest for CPI63767. A decrease in proteolytic activity as indirectly shown by a change in VFA composition favouring a shift towards propionate and reduction in branched‐chain VFA production varied with type of CT and was highest for CPI63767. In conclusion, these results suggest that tannin polymer size is an important factor affecting in vitro CH₄ production which may be linked to the CT interaction with dietary substrate or microbial cells.     

中国草地固碳减排研究现状及其建议

随着经济的快速发展,温室气体的排放量不断增加,加之人类对自然资源的利用强度逐渐增加,导致全球生态系统的固碳能力减弱,大气中的温室气体浓度达到新高,所造成的温室效应已经成为国际社会普遍关注的重大全球性问题。中国草地碳汇资源得天独厚,发展草原碳汇经济成为履行国际承诺、打造碳汇新经济、建设美丽中国的重要载体。综述中国草地固碳减排现状及其影响因素,包括草地碳汇和家畜生产减排研究、气候变化背景下的草地碳汇、人工草地建设等方面,并提出中国草地固碳减排发展建议,以期为中国实现碳达峰、碳中和及草地固碳减排的贡献提供理论基础,为推动我国社会高质量发展、创造高品质生活提供坚实的技术支撑。