当归茎线虫病田间分布型及抽样技术研究

为提高当归茎线虫病(Ditylenchus destructor)田间抽样的准确度,用7种聚集指标判断了当归茎线虫病的田间分布型.结果表明,当归茎线虫病在田间呈聚集分布,其中奈曼分布(核心分布)的适合率为100%,嵌纹分布(负二项分布)的适合率为77.8%.理论抽样数量约820株.4种抽样方法差异均不显著,在保证抽样数量合理的情况下,4种方法均可采用.     

Effect of EGFP gene transfection on the cell cycle distribution of primary cultured human chondrocytes

AIM: To investigate the effect of enhanced green fluorescence protein (EGFP) gene transfection on the cell cycle distribution of primary cultured human chondrocytes in order to establish a tracking method of cultured human nasoseptal chondrocytes. METHODS: pEGFP-N1 plasmid was amplified in E.coli, and purified by high purity kit. Primary cultured human chondrocytes,which were initially obtained from the nasoseptal cartilage, were cultured in vitro and transferred with pEGFP-N1 by means of electroporation with Amaxa nucleofector device. Transfering process and transient expression were evaluated by laser scanning confocal microscope (LSCM), the transfer efficiency and the cell cycle distribution were evaluated by flow cytometry. RESULTS: There was significant expression of EGFP at 24 h after transferring. The transfection efficiency of pEGFP-N1 into primary cultured human chondrocytes reached 35.37％ at 48 h. It didn't affect the process of cell adherance and had no effect on the cell cycle distribution. CONCLUSION: Primary cultured human chondrocytes, which were transfected with pEGFP, are alive in vitro, and the transferring process doesn't affect the cell cycle distribution. These results suggest that pEGFP-N1 is an ideal transient expression vector for primary cultured human chondrocytes and it might be a well tracer in construction tissue engineered cartilage.     

Roles of the Kv1.2, Kv1.5, Kv2.1 potassium channels in hypoxia pulmonary vasoconstriction

AIM: To determine the role of Kv1.2, Kv1.5, Kv2.1 in the hypoxia pulmonary vasoconstriction (HPV). METHODS: Male Wistar rats were divided into two groups: normoxic group and hypoxic group. The single smooth muscle cell was obtained from pulmonary artery of Wistar rats with acute enzymatic digestion method. The conventional whole-cell patch clamp technique was used to record the resting membrane potential (Em) and the potassium currents of voltage-gated potassium channel (IKv) in rat pulmonary arterial smooth muscle cells (PASMC). Intracellular application of Kv1.2/Kv1.5/Kv2.1 antibodies (1∶125) was conducted through the whole-cell patch clamp system. RESULTS: ① Em of PASMC was depolarized after 24 h hypoxia compared with that of control cells . IKv of PASMC was decreased after 24 h hypoxia, . ② The mixture of Kv1.2/Kv1.5/Kv2.1 antibodies depolarized Em and inhibited IKv in PASMC from normoxic rat, whereas the mixture of Kir2.1/Kir2.3/Kir4.1 antibodies had no effects on them. ③ The mixture of Kv1.2/Kv1.5/Kv2.1 antibodies and the mixture of Kir2.1/Kir2.3/Kir4.1 antibodies had no effects on IKv and Em from rats hypoxic for 24 h. CONCLUSION: Kv1.2, Kv1.5, Kv2.1 might be oxygen sensitive potassium channels which mediated HPV.     

Combining competition from Lolium perenne and an insect–fungus combination to control Rumex obtusifolius seedlings

Although adult Rumex obtusifolius are problematic weeds, their seedlings are poor competitors against Lolium perenne, particularly in established swards. We investigated the possibility of using this weakness to augment control of R. obtusifolius seedlings with combinations of Gastrophysa viridula (Coleoptera: Chrysomelidae) and the rust fungus Uromyces rumicis. Rumex obtusifolius seedlings were grown in competition with L. perenne sown at different rates and times after R. obtusifolius: they competed successfully with L. perenne when sown 21 days before the grass. Sowing both species at the same time resulted in a dominant grass sward, with R. obtusifolius becoming dominant when sown 42 days prior to L. perenne. Grass sowing rate had no effect on R. obtusifolius growth or biomass. A second experiment investigated how competition from L. perenne sown 21 days after R. obtusifolius combined with damage from G. viridula and/or U. rumicis (applied at either the 3–4‐ or 10–13‐leaf stage, or at both stages) affected the growth and final biomass of R. obtusifolius. Beetle grazing at the latter leaf stage was the only treatment that reduced R. obtusifolius biomass, although rust infection at the earlier application led to an increase in shoot and root weight. The results are discussed in terms of the potential for use of these agents in the field.     

植物育种的物理学方法

介绍了草原生态恢复中选育优良牧草种子的一些物理学方法,包括电场、磁场、物理辐射、激光、离子束和交变应力等在植物的生物学效应、诱变育种和转基因技术中的应用.     

Assessing exterior egg quality indicators using machine vision

ABSTRACT

1. The objective of this study was to develop a machine vision method for analysing exterior parameters of chicken eggs to automate the stage of primary sorting.

2. The developed algorithm based on predetermined thresholds calculated egg quality indicators, including geometric dimensions, shape index and the mottling grade. The algorithm was implemented with an experimental setup that combined the image-based and the candling methods. A total of 400 egg samples were analysed.

3. Comparison of results of the algorithm with those obtained using the traditional manual method showed that mean value of radii values difference was 0.095 ± 0.058 mm for the sharp and 0.080 ± 0.047 mm for the blunt end of the egg, with standard deviations of 0.58 mm and 0.49 mm, respectively.

4. The correlation coefficient between the shape index values determined by the two methods was 0.93; the standard deviation of absolute differences between corresponding values was 1.05%.

5. The results of mottling grade estimation were compared using F-measure and confusion matrix.

6. The results allow the possibility to perform the assessment of egg exterior quality factors in an automatic mode, independent of the expertise of a grader.     

The value of vegetation cover for ecosystem services in the suburban context

Latin-American cities can be characterized by dynamic processes of urbanization that encroach upon the natural and semi-natural surrounding landscapes. Our study presents the effects of landscape development, transformed from semi-natural conditions into a mostly disperse suburban settlement. We explore the impact that this transformation has had on this context by three ecosystem services that regulate rainwater runoff, enhance microclimate conditions and help to improve air quality by monitoring vegetation cover. We have designed a spatio-temporal hierarchical analysis which employs remote sensing techniques to capture the structural changes of this landscape over long, medium and short term scales on two spatial levels. This methodological approach was tested in the Metropolitan Area of Santiago (MAS) as case study area. Despite of the increase in impervious surfaces due to urban processes, there has also been an increase in vegetation cover, which has led to an improvement in the provision of the above-mentioned ecosystem services. Hence, if diverse urbanization processes continue and they are coupled with an increase in vegetation cover, the provision of ecosystem services could also expand. This phenomenon can be observed in some areas, where public and private green spaces are created and maintained. Our data analyses give evidence that certain types of suburban areas which increase the share of vegetation cover can provide daily ecological benefits for urban neighborhoods, and beyond, for adjacent areas. Moreover, suburban development can successfully provide ecological benefits to citizens. Such processes can only be ecologically sustainable if the composition of vegetation is well-adapted to the regional climatic conditions.     

蜡蝉科(半翅目：头喙亚目)昆虫起源及分布格局研究

【目的】探究蜡蝉科及其各亚科的分布格局与起源。【方法】根据世界动物地理分区,以搜集到的标本和权威文献记录为依据,建立蜡蝉科物种及地理分布数据库,采用Arc-GIS软件的地理信息处理功能对地理分布数据库进行运算,得到蜡蝉科物种地理分布图,用区系相似系数分析区域间物种的相似性。【结果】(1)蜡蝉科在新热带区分布的种类最多,达290种,其次是东洋区、非洲区和澳洲区,在新北区和古北区较少;(2)新热带区分布的属和特有属最多,非洲区次之,东洋区的特有属比例小于前2个大区,澳洲区分布属较少,但特有属比例很高,古北区分布的特有属明显较少,新北区无特有属分布;(3)新北区和新热带区蜡蝉科物种的相似系数为4.38,古北区与东洋区的相似系数为2.29,其他各区之间的相似系数都较低。蜡蝉科中Lyncidinae、Phenacinae、Poiocerinae和Strongylodematinae 4个亚科均起源于新世界;Aphaeninae、Xosopharinae和Zanninae 3个亚科起源于旧世界;Amyclinae、Dichopterinae、Enchophorinae和Fulgorinae 4个亚科可能分别起源于新世界和旧世界。【结论】各大区间蜡蝉科种类相似性系数高低与该科的地质演化历史密切相关。蜡蝉科可能起源于新世界和旧世界,起源时间可能在白垩纪早期,距今约135 Ma。     

Effects of remifentanil on monophasic action potential and transmural dispersion of repolarization in rabbit myocardium

AIM: To study the effect of remifentanil on monophasic action potential and transmural dispersion of repolarization (TDR) in the 3-layer myocardium of isolated rabbit hearts. METHODS: Adult rabbits (n=18, 2.0 ~ 2.5 kg) were used to isolate the hearts for preparing Langendorff perfusion model. The hearts were randomly divided into 3 groups after perfusion with K-H solution for 15 min: the perfusion in control group (C group) continued for 60 min; the hearts in remifentanil group (R group) were perfused with 12 μg/L remifentanil K-H solution for 60 min; the hearts in remifentanil+aminophylline group (RA group) were given 60-min perfusion of 12 μg/L K-H remifentanil+30 mg/L aminophylline. The HR and 3 layers of myocardial monophasic action potential (MAP) in the left ventricular anterior wall were recorded at time points after balanced infusion for 15 min (T₀), and continued perfusion for 15 min (T₁), 30 min (T₂) and 60 min (T₃). The monophasic action potential duration of repolarization at 90% (MAPD₉₀) and the transmural dispersion of repolarization (TDR) were calculated. The early afterdepolarization, delay afterdepolarization and arrhythmia were also observed. RESULTS: In R group, slower HR and prolonger MAPD₉₀ and TDR at T₁~T₃ were observed as compared with those at T₀ (P<0.05). R group showed slower HR and longer MAPD₉₀ and TDR than C group and RA group (P<0.05). CONCLUSION: Remifentanil slows the HR, extends the MAPD₉₀ and increases the TDR, thus being prone to induce reentry. Aminophylline makes HR faster and MAPD₉₀ shorter, thereby reducing the TDR.     

从可持续发展浅谈森林保护与利用对策——以森林生物多样性保护与病虫害控制为例

通过分析森林生物多样性与森林病虫害的现状及其对森林可持续发展的意义,提出了提高意识、加强管理,依靠科技手段保护生物多样性和防治病虫害等措施建议,以期可持续发展森林保护与利用。