全文获取类型
收费全文 | 333篇 |
免费 | 12篇 |
国内免费 | 14篇 |
专业分类
林业 | 11篇 |
农学 | 14篇 |
基础科学 | 9篇 |
10篇 | |
综合类 | 60篇 |
农作物 | 6篇 |
水产渔业 | 14篇 |
畜牧兽医 | 68篇 |
园艺 | 164篇 |
植物保护 | 3篇 |
出版年
2023年 | 2篇 |
2022年 | 3篇 |
2021年 | 8篇 |
2020年 | 10篇 |
2019年 | 16篇 |
2018年 | 5篇 |
2017年 | 19篇 |
2016年 | 19篇 |
2015年 | 13篇 |
2014年 | 10篇 |
2013年 | 24篇 |
2012年 | 17篇 |
2011年 | 16篇 |
2010年 | 16篇 |
2009年 | 3篇 |
2008年 | 14篇 |
2007年 | 22篇 |
2006年 | 7篇 |
2005年 | 6篇 |
2004年 | 18篇 |
2003年 | 14篇 |
2002年 | 23篇 |
2001年 | 15篇 |
2000年 | 18篇 |
1999年 | 4篇 |
1998年 | 3篇 |
1997年 | 3篇 |
1996年 | 6篇 |
1995年 | 2篇 |
1994年 | 3篇 |
1993年 | 6篇 |
1992年 | 4篇 |
1991年 | 2篇 |
1990年 | 2篇 |
1989年 | 3篇 |
1988年 | 2篇 |
1976年 | 1篇 |
排序方式: 共有359条查询结果,搜索用时 31 毫秒
61.
本文报导了国家注册的奇台无芒雀麦地方品种(Bromus inermis L(?)yss.CV.Qitai)的品种来源及分布,品种特征特性以及主要栽培技术和利用方法,为该品种的进一步推广提供了科学依据。 相似文献
62.
AIM: To investigate the effect of nucleolin on angiotensin II (Ang II)-induced phenotypic transformation of vascular smooth muscle cells (VSMCs). METHODS: Ang II was used to induce the phenotypic transformation of VSMCs. The spatial and temporal expression patterns of nucleolin, and the effects of Ang II on the expression of VSMC phenotypic transformation markers α-smooth muscle actin (α-SMA), calponin, smooth muscle protein 22 α (SM22α) and osteopontin (OPN) were investigated. The techniques of gene over-expression and RNA interference were used to assess the effect of nucleolin on the expression of Ang II-mediated VSMC phenotypic transformation markers. RESULTS: The expression of α-SMA, SM22α and calponin at the mRNA and protein levels was gradually decreased by Ang II stimulation, while the expression of OPN at mRNA and protein levels was gradually increased. The expression of nucleolin was gradually up-regulated in the VSMCs treated with Ang II at different concentrations for various duration (P<0.05). Ang II induced nucleolin translocation from the nucleus to cytoplasm. Over-expression of nucleolin promoted the VSMC phenotypic transformation induced by Ang II. Down-regulation of nucleolin suppressed the promotion of phenotypic transformation. CONCLUSION: Nucleolin promotes Ang II-induced phenotypic transformation of VSMCs, and its mechanism may be related to its function of cytoplasmic translocation. 相似文献
63.
Alessia Diana DVM PHD Marco Pietra DVM Carlo Guglielmini DVM Andrea Boari DVM Giuliano Bettini DVM Mario Cipone DVM 《Veterinary radiology & ultrasound》2003,44(5):566-569
The ultrasonographic findings for four cats with intestinal smooth muscle hypertrophy are described. In two cats, intestinal smooth muscle hypertrophy was associated with chronic enteritis. In the remaining two cats, intestinal smooth muscle hypertrophy affected the intestinal tract proximal to stenosis due to alimentary lymphoma and an intestinal foreign body, respectively. Moderate increased thickness of the affected intestinal wall, measuring 7-8 mm, was evident on abdominal ultrasonographic examination of all subjects. In addition, the ultrasonographic five-layered feature of the intestinal wall was maintained, and only the muscular layer appeared thickened. Abdominal ultrasound allowed a presumptive diagnosis of intestinal smooth muscle hypertrophy that was confirmed histologically in all cats. 相似文献
64.
催产素能使妊娠 1 5d后的母兔子宫出现收缩反应。但在妊娠母兔受到强应激刺激后立即注射催产素子宫不发生收缩。说明当应激达到一定强度时 ,母兔因紧张所分泌的肾上腺素足以封闭子宫平滑肌上的催产素受体 ,从而使催产素在一定的时间内不能使子宫收缩 相似文献
65.
Yasuyuki TANAHASHI Ban WANG Yuri MURAKAMI Toshihiro UNNO Hayato MATSUYAMA Hiroshi NAGANO Seiichi KOMORI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):203-211
In order to investigate the effects of (SKF), which is a non-selective cationic channel blocker, on
K+ channel currents, we recorded currents through ATP sensitive K+ (IKATP),
voltage-gated K+ (IKv) and Ca2+ activated K+ channels
(IBK) in the absence and presence of SKF in single small intestinal myocytes of mice with
patch-clamp techniques. SKF (10 µM) reversibly abolished IKATP that was induced by
cromakalim (10 µM), which is a selective ATP sensitive K+ channel opener. These
inhibitory effects were induced in a concentration-dependent and voltage-independent manner. The 50%
inhibitory concentration (IC50) was 0.85 µM, which was obviously lower than that
reported for the muscarinic cationic current. In addition, SKF (1 µM ≈ the IC50
value in IKATP suppression) reversibly inhibited the IKv that was induced by repetitive
depolarizing pulses from −80 to 20 mV. However, the extent of the inhibitory effects was only ~30%. In
contrast, SKF (1 µM) had no significant effects on spontaneous transient IBK and
caffeine-induced IBK. These results indicated that SKF inhibited ATP sensitive K+
channels and voltage-gated K+ channels, with the ATP sensitive K+ channels being more
sensitive than the voltage-gated K+ channels. These inhibitory effects on K+ channels
should be considered when SKF is used as a cationic channel blocker. SKF96365相似文献
66.
PEI Xing HAN Yong ZHANG Zhan-hua LI Na SHI Yao ZHANG Yuan-yuan FAN Yi-gang TIAN Hong-yan 《园艺学报》2016,32(7):1174-1179
AIM: To investigate the effects of sinapic acid(SA) on the proliferation and apoptosis of rat vascular smooth muscle cells(VSMCs) induced by high glucose(HG). METHODS: Cultured A7r5 cells were randomly divided and treated as indicated. The cell viability was determined by MTT assay. DNA synthesis was measured by BrdU assay. Cell cycle progression and cell apoptotic rate were determined by flow cytometry analysis. The levels of reactive oxygen species(ROS) were detected by ELISA. The protein levels of cyclin D1, P21, P27, phosphorylated protein kinase C(p-PKC), p-P38 and β-actin were evaluated by Western blot. RESULTS: Compared with control group, the viability of A7r5 cells was significantly enhanced, the DNA synthesis was increased, the cell cycle progression was promoted, the levels of ROS were elevated, the cell apoptotic rate was reduced, the protein expression of P21 and P27 was decreased, and the protein levels of cyclin D1, p-PKC and p-P38 were increased in HG group(all P<0.05). These effects were reversed by SA(0.1, 1 and 10 μmol/L) treatment in a dose-dependent manner(all P<0.05). Both P38 inhibitor SB203580 and PKC inhibitor chelerythrine significantly inhibit HG-induced PKC/P38 activation and cell viability(P<0.05).CONCLUSION: SA inhibits HG-induced VSMCs proliferation and promotes cell apoptosis via reducing PKC/P38 activation. 相似文献
67.
Effects of EGLN1 siRNA on growth of rat pulmonary artery smooth muscle cells under hypoxic condition
AIM: To observe whether EGLN1 gene is involved in the growth of pulmonary arterial smooth muscle cells (PASMCs) during hypoxia when EGLN1 gene expression was interference by siRNA. METHODS: The rat primary pulmonary arterial smooth muscle cells were cultured, and the specific lipidosome of EGLN1 siRNA was constructed and transfected into the PASMCs. The transfected PASMCs were cultured under hypoxia or normoxia conditions, respectively. The viability of the PASMCs was detected by CCK-8 assay. The protein expression of EGLN1 and vascular endothelial growth factor (VEGF) was determined by Western blot. RESULTS: The viability of the PASMCs was increased and the protein expression of VEGF was up-regulated in the PASMCs under hypoxic condition in a time-dependent manner. In hypoxia or normoxia condition, the viability and VEGF protein expression of the PASMCs were suppressed by EGLN1 siRNA. CONCLUSION: EGLN1 gene may involve in the growth of rat PASMCs by regulating VEGF protein level under hypoxic condition. 相似文献
68.
MU Da HE Fang REN Jiang-lin ZHANG Hui-min ZHONG Hua DENG Feng-mei SUN Zhi-ping 《园艺学报》2008,24(4):701-706
AIM: To investigate whether Smad pathway participates the process of extracellular signal regulated kinase (ERK) induced the proliferation of vascular smooth muscle cells (VSMCs). METHODS: Human umbilical artery smooth muscle cells (hUASMCs) were divided into four groups: control group, PDGF (platelet derived growth factor) group, ERK blocking agent group and PDGF+ERK blocking agent group. MTT assay was used to detect the proliferation of hUASMCs (A value). Immunohistochemical technique was used to detect the expression of PCNA, phosphorylated ERK (p-ERK) and phosphorylated Smad2/3 (p-Smad2/3) protein in hUASMCs. The expression of Smad2/3 mRNA in hUASMCs was detected by RT-PCR. RESULTS: The proliferation of hUASMCs and the expression of PCNA, p-ERK and p-Smad2/3 proteins in hUASMCs in PDGF group were increased obviously than those in other groups (P<0.01). No difference in the expression of Smad2/3 mRNA in hUASMCs among groups was observed. CONCLUSION: Smad pathway participates the process of ERK pathway that induces the proliferation of hUASMCs at the level of protein. 相似文献
69.
70.