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61.
甘肃地方苜蓿农家品种脯氨酸积累能力与抗旱性的研究 总被引:8,自引:1,他引:7
本文依据“脯氨酸维持积累时间”和“脯氨酸维持积累量”指标评介了四个甘肃地方苜蓿农家品种的抗旱能力。结果表明:敦煌苜蓿的抗旱能力高于会宁、秦安、灵台苜蓿,其中会宁苜蓿居二,灵台苜蓿抗旱性最差。这一抗旱排序与叶片相对含水量及气孔指标所得到的结果相一致。证明了“脯氨酸维持积累时间”和“脯氨酸维持积累量”可以作为首蓿抗旱性的鉴定指标。 相似文献
62.
Controlling established horsenettle plants is achieved by suppressing shoot emergence from root systems. The seasonal pattern of shoot emergence and its possible endogenous control in horsenettle ( Solanum carolinense L.) were investigated. The shoot emergence period in an undisturbed population was limited to a seven-week period from mid-April, and a little longer in tilled conditions. Detached roots showed very high shoot-sprouting ability under 15–30°C throughout the year. In shoot clipping experiments, new shoots sprouted only from the stem and not from the root when attached to shoots, whether above-ground or underground. On the contrary, new shoots sprouted from the roots when all parts of the shoots were clipped off. From these results, the limited shoot emergence period in horsenettle is thought to be initiated by temperatures necessary for sprouting and is ended by a growth correlation effect between early emerged and matured shoots. 相似文献
63.
在对甘肃荚蒾属植物资源调查的基础上, 整理并描述了27个分类群的生境、分布以及观赏性状, 包括叶、花、果实等, 并结合该属植物的资源现状, 提出了可持续开发利用的建议。 相似文献
64.
黄瓜全雌性基因连锁的AFLP和SCAR分子标记 总被引:32,自引:5,他引:32
本研究以全雌品种‘戴多星’自交系和弱雌品种‘北京截头’自交系为双亲杂交获得F1 ,然后得到F2 性型分离群体, 利用分离群体分组分析法(Bulked Segregant Analysis, BSA) 构建全雌和弱雌两个基因池, 筛选了64对AFLP选择性引物EcoR I-NN +Mse I-NNN组合, 发现EcoR I-TG +Mse I-CAC引物组合在全雌基因池中扩增出一条分子量为234 bp的特异带。经F2 代单株验证, 该特异条带能在全雌单株中稳定出现。以MAP MAKER (Version 310) 软件分析, 该标记与全雌性位点的连锁距离在617 cM。命名该连锁标记为TG/CAC234。将该特异条带回收、克隆、测序, 设计特异SCAR引物, 再对F2 代单株基因组DNA进行扩增, 仅在全雌单株中扩增出1条分子量为166 bp 的特异带, 表明已成功地将与黄瓜全雌性连锁的AFLP标记转化为操作简便、表现稳定的SCAR标记, 该标记命名为SA166。 相似文献
65.
从甜瓜子叶中提取总DNA 总被引:4,自引:1,他引:4
用分子标记方法检测甜瓜杂交种子纯度,如果从真叶中提取DNA,检测周期至少需要15d。为了缩短检测周期,我们分别以甜瓜的成熟干种子、吸胀水后“露白”种子、3d龄刚转绿子叶、6d龄子叶、9d龄子叶和12d龄子叶为材料,进行了提取总DNA的研究。结果表明:除了干种子和吸胀水后“露白”种子外,其他的材料都可以提取到DNA,但是DNA的质量因子叶日龄不同而存在很大的差异。不同日龄的子叶中,以3d龄子叶提取的DNA质量好。子叶6d龄时,提取的DNA质量次于3d龄,少量DNA开始出现降解,以后随着子叶日龄的增加,DNA降解加重。另外,与以真叶为材料提取的DNA样品相比较,子叶DNA样品中的蛋白质等杂质含量高,应增加氯仿抽提纯化次数。 相似文献
66.
67.
Xanthomonas campestris pv. vitians , the causal agent of bacterial leaf spot of lettuce (BLS), can be seedborne, but the mechanism by which the bacteria contaminates and/or infects lettuce seed is not known. In this study, the capacity of X. campestris pv. vitians to enter and translocate within the vascular system of lettuce plants was examined. The stems of 8- to 11-week-old lettuce plants were stab-inoculated, and movement of X. campestris pv. vitians was monitored at various intervals. At 4, 8, 12 and 16 h post-inoculation (hpi), X. campestris pv. vitians was recovered from 2 to 10 cm above (depending on stem length) and 2 cm below the inoculation site. Xanthomonas campestris pv. vitians was also recovered from surface-disinfested stem sections of spray-inoculated plants. Together, these results are consistent with X. campestris pv. vitians invading and moving systemically within the vascular system of lettuce plants. To investigate the mechanism of seed contamination, lettuce plants at the vegetative stage of growth were spray-inoculated with X. campestris pv. vitians and allowed to develop BLS. Seed collected from these plants had a 2% incidence of X. campestris pv. vitians external colonization, but no bacteria were recovered from within the seed. 相似文献
68.
Summary Stem thickness of the weed Solanum nigrum and the crop sugarbeet was determined with a He–Ne laser using a novel non‐destructive technique measuring stem shadow. Thereafter, the stems were cut close to the soil surface with a CO2 laser. Treatments were carried out on pot plants, grown in the greenhouse, at two different growth stages, and plant dry matter was measured 2–5 weeks after treatment. The relationship between plant dry weight and laser energy was analysed using two different non‐linear dose–response regression models; one model included stem thickness as a variable, the other did not. A binary model was also tested. The non‐linear model incorporating stem thickness described the data best, indicating that it would be possible to optimize laser cutting by measuring stem thickness before cutting. The general tendency was that more energy was needed the thicker the stem. Energy uses on a field scale are discussed. 相似文献
69.
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