苹果试管苗不定根起源及其发育状况的研究

利用光学和扫描电子显微镜观察结果表明,经琼脂生根培养基诱导产生的长富—2苹果 (Malus pumila Mill.) 试管苗的不定根起源于维管形成层。这种不定根发育不良,根表面缺乏根毛,内部解剖构造为典型的初生结构。为避免因不定根吸收、运输能力差而影响试管苗移栽成活,宜采用通透性良好的基质进行瓶内或瓶外生根,可诱导出优质不定根。     

Horizontal transmission and host-virulence attenuation of totivirus in violet root rot fungus Helicobasidium mompa

Monokaryotic strains of Helicobasidium mompa were used as vectors of a mycovirus between various H. mompa isolates to examine the transmissibility of one of the mycoviruses, totivirus (HmTV1–17 virus) in the hypovirulent isolate V17 of H. mompa. The isolates that acquired HmTV1–17 virus were also examined for any alteration in the virulence. Twelve dikaryotic isolates of H. mompa, belonging to 11 mycelial compatibility groups (MCGs) and being mycelially incompatible with isolate V17, were used as recipients of HmTV1–17 virus. Two monokaryotic isolates that were mycelially incompatible with isolate V17 and all of the recipients were also used as vectors of HmTV1-17 virus between isolate V17 and the recipients. When isolate V17 and recipients were directly paired on plate media, HmTV1-17 virus was transmitted from isolate V17 into 2 of the 12 recipients (i.e., 2 of the 11 MCGs). Two monokaryotic strains were paired with isolate V17, and the monokaryotic strains that acquired HmTV1-17 virus were then used as new virus donors. When the monokaryotic strains containing HmTV1-17 virus were paired with the 12 recipients, HmTV1-17 virus was transmitted into 7 of the 12 recipients from the monokaryotic strains (i.e., 7 of 11 MCGs). Based on these results, we concluded that monokaryotic strains could act as vectors to transmit HmTV1-17 virus into H. mompa isolates. When four of the H. mompa isolates that acquired HmTV1-17 virus were used to inoculate apple rootstock Malus prunifolia, the virulence of all of the isolates was attenuated from that of their parental isolates. Moreover, because the DNA fingerprints of the fungal isolates that acquired HmTV1-17 virus were the same as those of their parental isolates, the infection with HmTV1-17 virus is considered the cause of virulence attenuation of H. mompa.     

Fusarium oxysporum对苗期大豆根部的致病性和侵染规律

Fusarium oxysporum对苗期大豆具有较强的致病作用。病原菌自伤口或直接侵入大豆幼苗根部的皮层组织,随着病原菌的侵入和扩展,细胞出现坏死,组织发生崩解,根部组织表现红褐色病斑。环境因素对病原菌的侵染时期和在大豆体内潜育时间的长短有着明显的影响。侵染时期主要受病原菌侵入前自身所处状态和寄主的影响,侵入后潜育时间的长短则受植株状态的影响。土壤环境条件直接影响着病原菌的侵入和寄主的抗病性,间接影响着病原菌在大豆植株体内的扩展和病症的表现。     

The Effect of Detopping the Field Bean Plants (Vicia faba L. minor) and Lack of the Pod Setting on the Symbiosis with Root Nodule Bacteria

The plants of field bean var. Nadwiślaνski were detopped after about 1 month since the beginning of flowering and after that all flower buds were removed from some plants. The effect of a change in the trophic balance on the setting and subsequent growth of root nodules was studied.
The removal of pods had the greater effect on the increase in the weight of the remaining organs than the detopping of plants. Detopping retarded the ageing of the plants and delayed the phase of the greatest reduction of the number of pods. The detopping of plants revealed their considerable compensative possibilities in terms of production of the pod weight and the potential of leaves for biomass production. The data obtained indicate that plant detopping may disturb the root–bacteria exchange of signals, necessary to initiate the nodule development, while the further growth of nodules depends, above all, on the amount of available photosynthates.
A very high correlation between the dry weight of the whole plant to the dry weight of leaves ratio and the weight of nodules was observed. It proves the importance of the trophic balance of the plant for the root nodule weight production.     

用简易诊断法检查判断液压系统各元件的工作性能

故障的排除首先在于判断，目前基层维修网点大多数没有检测仪器和设备，出了故障乱拆乱卸，导致液压元件工作失灵，甚至整车液压系统瘫痪。拖拉机液压系统产生故障的主要原因是液压元件工作性能恶化引起的。为此，介绍了在没有检测仪器和设备的条件下，如何应用简易诊断法检查判断各液压元件的工作性能。     

手动比例复合阀在中高压液压绞纲机中的应用

中高压液压技术在海洋捕捞机械—绞纲机中的使用推动了我国渔业机械的发展,手动比例复合阀在中高压液压技术上的应用则大大加快了液压绞纲机在国内渔业生产中的普及。目前,我国浙江、江苏、福建、广东和山东等省沿海渔民使用的绞纲机普遍采用带手动比例复合阀的液压系统。为此,对手动比例复合阀换向、溢流、调速集成等功能进行了分析。     

中国野生种毛葡萄光合特性的研究

毛葡萄（ＶｉｔｉｓｑｕｉｎｑｕａｎｇｕｌａｒｉｓＲｅｈｄ）的净光合速率日变化值明显高于山葡萄、宝石、白玉霓，且无栽培品种光合作用的“午休”现象。毛葡萄的净光合速率与其它野生种葡萄和栽培品种比较也表现出较高水平。在进行ＣＯ２饱和点和补偿点测定时，毛葡萄也较栽培品种白玉霓有较强的光合作用，因此，毛葡萄是葡萄高光合效率育种的优良野生种质资源。     

Response of Vitis vinifera cv. ‘Tempranillo’ to partial rootzone drying in the field: Water relations, growth, yield and fruit and wine quality

This paper reports the effects of irrigation amount and partial rootzone drying (PRD) on water relations, growth, yield and wine quality of Vitis vinifera cv. ‘Tempranillo’ during two consecutive years in a commercial vineyard with a deep, light-clay soil located in Requena, Valencia, Spain. Partial rootzone drying applied at two amounts (100% and 50% of the estimated crop evapotranspiration), was compared to conventional drip irrigation, and also to rainfed vines. Results showed that the effects of irrigation amount on yield and wine quality were different between years. In 2003 with low yield values (around 6.3 t ha⁻¹) irrigation did neither affect grape production nor wine quality. However, in the following year, with much higher general yield (17 t ha⁻¹), the high irrigation dose increased yield by 30% compared to rainfed vines and it also increased must total soluble solids and wine alcohol content. In both seasons, PRD did not significantly affect physiological parameters, nor growth, yield or fruit and wine quality, when compared to the same amount of water applied by conventional drip irrigation. Overall these results suggest that, under our experimental conditions, it was the irrigation amount rather than the system of application what affected vine performance, indicating the difficulties of successfully employing the PRD type of irrigation with a drip system in heavy and deep soils.     

农作物抗旱性鉴定仪的研制和应用

依据陈毓荃等首次提出的连续升温电导法，研制了ＮＫＪ－１型农作物抗旱性鉴定仪，对１４种小麦抗旱性的鉴定结果与田间观测结果一致，也适于果树抗热性鉴定。     

农机液压技术的应用与发展

液压技术的应用已成为现代化农业机械的典型标志。为此,介绍了农机液压技术的应用现状;并根据科学技术的发展,简要分析了农机液压技术的发展趋势。