马惠宁输油管道清管的风险分析及对策

介绍了马惠宁输油管道的施工和投产后的运行现状。由于多年运行，管道腐蚀严重，为此拟采用清管器进行内检测。对用清管器进行内检测可能产生的风险进行了分析，并提出了减小风险的对策。     

四川乐山地区紫茎泽兰的入侵定殖和风险评估

本文报道了四川省乐山地区犍为县塘坝乡境内入侵新物种一紫茎泽兰的分布、发生和定殖入侵情况。结合紫茎泽兰的生物学特性和当地的地理气候特征，对紫茎泽兰入侵乐山及其近邻地区的风险进行评估。     

发展我国洪水保险的制度思考——美国洪水保险制度的借鉴与启示

洪水保险是一项重要的非工程防洪措施.美国是世界上最早建立国家强制性洪水保险体制的国家,经过艰难曲折的探索,闯出了一条成功之路.我国的洪水保险体制还处于积极探索阶段.中美两国尽管国情差异较大,但以洪水保险作为推进洪泛区洪水风险管理的经济手段,并以立法和国家计划等措施来保障实施的作法是值得借鉴的.本文就美国洪水保险制度的发展与沿革,寻找对我国建立洪水保险制度一些有益的启示,并就此提出了一些建设性意见.     

影响老瓜头泌蜜量主导因素的研究

老瓜头自然分布于沙漠地带。在老瓜头开花期,天气变化无常。我们研究了对老瓜头泌蜜量起关键影响作用的气象因子。根据当地气候的变化规律进行预测预报,指导养蜂者正确选择场地,以夺取老瓜头蜜高产。     

油气管道风险管理技术现状及对策

介绍了国内外油气管道风险管理技术的研究现状、应用成果及最新的发展趋势,对比分析了国内外油气管道风险管理技术的差距,强调了油气管道风险管理的重要性,指出了油气管道风险技术的发展方向,为油气管道安全运行提供了技术支持.     

大肠杆菌毒力因子研究概况

致病性大肠杆菌的毒力因子主要有黏附素、毒素、外膜蛋白 ( OMP)及铁转运系统等。在大肠杆菌侵袭宿主组织并引起其发病的过程中 ,这些毒力因子相互协调、发挥作用。文章对毒力因子的致病作用、致病机理、免疫原性及其在疾病防制中的应用几个方面的研究概况作了综述 ,为防制大肠杆菌病、研制新型高效的大肠杆菌疫苗提供了理论依据。另外 ,文章还介绍了近年来已相继研制成功的全菌体疫苗、纯化菌毛疫苗、单价或多价基因工程菌毛疫苗以及重组肠毒素双价基因工程苗、OMP亚单位疫苗等。展现了新型疫苗的研制 ,给防制大肠杆菌病带来了新的前景以及大肠杆菌作为工程菌的应用对畜禽其他疾病防制的推动作用     

Effect of the Pietrain breed used as terminal boar on Post-weaning Multisystemic Wasting Syndrome (PMWS) in the offspring in four PMWS-affected farms

The aim of this study was to determine the potential risk factors for PMWS at the individual pig level and assess the effect of the Pietrain paternal genetic background of the animals in a cohort study. The survey was set up in four PMWS-affected farms with 2 repetitions (batches) per farm. A representative sample of 60 pigs per batch, stratified according to the paternal genetic background (Pietrain: yes vs. no), was randomly selected after farrowing. The representative cohort was divided into 8 batches and the pigs were individually monitored from birth to slaughter. Survival analysis was used to determine the factors related to the time to PMWS. The litter-cluster effect was taken into account using the marginal Cox model (robust estimation of the covariance matrix) and the gamma shared frailty model which were compared.No protective effect of the Pietrain breed on the time to PMWS and the proportion of affected pigs in the offspring was found in this study. Piglets showing low circovirus type 2 (PCV2) titres at 7 weeks-old with no subsequent seroconversion and piglets from PCV2 negative sows were most likely to be affected by PMWS (HR=7.0 and 2.8, respectively). Active infection of the pregnant dams with parvovirus was related to an increased risk of PMWS in the offspring (HR=2.3). Neck injuries due to poorly performed injections in the dams were associated with an increased risk of PMWS with the marginal model (HR=2.1). Oxytocin injection (dams) during farrowing was protective against PMWS in the offspring (HR=0.6).     

荔枝生产相关的水分生理指标远程监测研究

利用植物远程监测系统对荔枝园中的大气温湿度、土壤湿度、光照强度、大气蒸汽压差(VPD)等环境因子和茎干直径微变化、果实生长、叶片温度等树体生理指标进行了监测,发现该系统能准确及时并无伤害地记录他们的实时和周期性变化。据获得的数据初步表明,当果实处于快速膨大期,主茎的加粗生长明显缓慢,一旦果实采收后,茎干加粗生长则有一个迅速上升的变化;当土壤湿度高于33%(v/v)时,主茎的生长受到明显的抑制;VPD白天上升,夜间下降,当夜间VPD始终高于0,就形成所谓的“夜间干燥”,VPD的这种变化对荔枝主茎和果实的生长以及叶气温差(LATD)都有较大的影响,如主茎和果实的日间收缩量随VPD的增大而增大,夜间空气干燥不但抑制了主茎生长,也使日最大LATD下降。据此认为,果实和茎干的生长之间存在竞争关系,土壤湿度过高或者夜间空气干燥对荔枝的生长不利。     

Antisense drug targeting with VEGF mRNA designed by computer aid and inhibitory effects on K562 cell line in vitro

AIM:The effective antisense sequences targeted VEGF mRNA with computer software would be screened and designed, and effect of them on growth K562 cells and protein expression of VEGF were studied with experiments.METHODS:Seven antisense sequences were selected and synthesized, which consisted of 18-20 deoxynucleotide acid and were modified with phosphorothioate, according to principle of low free energy of overall △G₃₇ Overall. Cell growth was assayed by trypan blue dye exclusion assay and level of VEGF protien in the media was determined by ELISA.RESULTS:Six of seven sequences were capable of inhibing growth of K562 cells and downregulating the VEGF protein expression significantly, compared with Scrambed control group. It was found that there was a close correlation between low level of overall △G₃₇ and antisense effectiveness (r=0.887,P<0.01).CONCLUSION:VEGF mRNA antisense oliogdeoxynucleotides, which were designed by computer software of RNAstructure, were able to inhibit growth of K562 and its protein expression. The VEGF mRNA may be new target attached by drugs. At same time, the computer aided design is useful methods to obtain the effective antisense.     

苏云金杆菌小试生产发酵影响因子的研究

利用 1 0L全自动发酵罐对苏云金杆菌小试生产的发酵条件进行了研究。结果显示 ,不同的温度、pH值、培养基成分及接种方式对发酵产量和发酵周期均有影响 ;在培养基配方中存在葡萄糖时 ,葡萄糖单独灭菌对提高苏云金杆菌发酵产量是重要的。初步生物测定显示 ,发酵上清中存在一些杀虫增效因子 ,建议配制苏云金杆菌制剂时尽可能加以利用。