文心兰类原球茎的诱导与增殖技术的优化

以文心兰试管苗叶片为材料,在(25±1)℃,光照强度24～30μmol/m2.s,光照时间10 h/d下研究类原球茎的诱导和增殖技术。结果表明:①蔗糖适于作为类原球茎诱导的碳源,类原球茎诱导率52%,单位叶片类原球茎2.725个;②附加3～5 mg/L的Ad处理有利于类原球茎诱导(诱导率57%,单位叶片类原球茎数量3.725～4个);③6-BA是影响文心兰类原球茎增殖的主导因子,中等浓度的6-BA(3.0 mg/L)有利于类原球茎的增殖,增殖率(15.29)极显著高于其它水平;④培养基1/2MS+6-BA 3.0 mg/L+2,4-D 1.0 mg/L+NAA 0.4 mg/L+蔗糖20 g/L最有利于文心兰类原球茎的增殖,增殖率最高(15.95),极显著高于其它处理组合。     

Exclusion of cytoplasmic fragments in flow cytometric analysis of lymph node samples from dogs with lymphoma using membrane-permeable violet laser-excitable DNA-binding fluorescent dye (DyeCycle Violet)

Background: Cytoplasmic fragments derived from fragile neoplastic lymphocytes are common in samples of lymph nodes collected from dogs with lymphoma. These cytoplasmic fragments interfere with accurate gating of target cells and quantification protocols used for flow cytometry because of their variable size and expression of lymphoid cell surface antigens on their membranes. Objective: The aim of this study was to develop a method to efficiently exclude cytoplasmic fragments from flow cytometric analysis of canine lymph nodes in which lymphoma was present. Methods: Single‐cell suspensions of neoplastic cells were prepared from biopsy samples and fine‐needle aspirates of lymph nodes from 23 dogs with lymphoma. Suspensions were stained using a violet laser‐excitable (405 nm) membrane‐permeable DNA‐binding fluorescent dye (DyeCycle Violet [DCV]), incubated with antibodies against CD3, CD5, CD21, CD22, and CD45, and then stained with 7‐amino‐actinomycin D (7‐AAD), an argon‐excitable (488 nm) membrane‐impermeable DNA‐binding fluorescent dye. Multiparameter flow cytometry was used for analysis based on selective uptake and laser‐activated fluorescence of these dyes. Results: Cytoplasmic fragments, which were DCV‐negative and CD45‐positive, and dead cells, which were positive for 7‐AAD, were efficiently separated from neoplastic cells. Conclusion: Staining with DCV is a useful method to improve flow cytometric gating methods and quantitative analyses of lymph node samples from dogs with lymphoma.     

车身非光滑表面组合布置对气动特性影响分析

为了研究非光滑表面尺寸及组合布置位置对汽车气动性能的影响.以MIRA阶梯背模型为研究对象,采用CFD与风洞试验相结合的方法对3种不同位置组合模型的气动性能进行了研究,并与光滑表面模型进行对比分析,探讨其减阻机理.结果表明,行李舱盖,车身尾部和车身底部组合布置非光滑单元体减阻效果最佳,减阻率为5.90%.非光滑表面通过改善汽车的尾部涡流,降低了模型压差阻力;同时通过改变近壁面气流的流动状态,降低了车身表面的气流速度,减小车身的摩擦阻力.     

X线平片检查对犬胃内不同性质异物诊断价值的研究

犬胃内异物是犬常见病、多发病,对其准确的诊断是兽医临床实施有效治疗的主要环节.文章利用X线机对犬胃内9种异物病例进行X线平片的拍摄观察,并对犬胃内大小、形状、密度不同的异物(如棉花团、木块、实心塑料球、骨头块、金属管等)的诊断效果进行了归类、比较和分析.结果显示,X线平片检查能够清晰、直观地显示胃内高密度异物,并且能够对其准确地定位;但对于胃内低密度异物的显像效果不明显,甚至观察不到胃内异物的存在;胃内中等密度的异物虽然能够通过X线显示,但其轮廓不十分清晰.     

常熟地区河湖水体的氮污染源研究

利用GPS定位的方法在丰水期和枯水期对常熟地区境内12条河流和3个湖荡水体氮磷污染现状进行了调查研究,对常熟地区主要河湖地表水中不同形态氮浓度的季节性变化特征进行了初步探讨。根据地表水中不同形态氮的浓度及总磷、溶解磷的浓度,结合15N示踪法和大型原状土柱渗漏池试验进一步补充探明了对该地区河湖水体中的氮磷污染来源。研究发现,该地区河、湖水体氮磷污染已相当严重,现阶段氮磷污染源主要来自城镇生活污水、农村人畜排泄物,在此基础上提出了缓解该地区水体氮磷污染的相应对策。     

波斯顿蕨离体培养技术的研究

以波斯顿蕨走茎茎尖为外植体研究无菌培养的繁殖方法,结果表明,最佳诱导培养基为MS+BA0.5,增殖培养基为MS+BA0.7,增殖系数为7.2;孢子体分化培养基为MS+BA1.0+NAA0.5,诱导率可达到92% ;适宜的生根培养基为1/2MS+BA1.0+IBA0.2+0.1% 活性炭,生根率为100%。     

文心兰茎尖诱导原球茎的影响因子研究

以文心兰无菌幼苗茎尖为试材,研究了基本培养基、植物生长调节剂、碳源、培养基pH值及茎尖放置方式对原球茎诱导的影响。结果表明:1/2MS为原球茎诱导的适宜培养基,0.1 mg/L TDZ最适合原球茎诱导且无不定芽分化、诱导率达80%,30 g/L蔗糖是原球茎诱导的适宜碳源,培养基pH值为5.2时原球茎诱导效果最佳,茎尖竖直放置比水平放置更有利于原球茎诱导。     

长春市农业产业结构调整与优化的经济学思考

长春市农业产业结构功能尚未充分发挥出来,产生的原因是市场主体－农民、加工企业、中介组织发育不成熟,政府职能、城市建设、科技进步等外在环境有待于改善。调整与优化长春市农业产业结构的关键是培养市场主体,改善外部环境。