光照对鸡产蛋性能的影响及其机理研究——Ⅲ.光照对鸡大脑皮质电位的影响

选用22只“星杂579”成龄母鸡,以电生理方法观察了光照(30lx)及在麻醉状态下光照(30lx)和黑暗对其大脑皮质“Wulst”区电位的影响。结果,黑暗时的电位可因光照而抑制;注射氯胺酮麻醉后,电位明显变小,再给黑暗和光照,电位均没有变化。表明大脑皮质参与了光照对鸡的生理调节过程。     

Development of a semi-selective medium and an immunofluorescence colony-staining procedure for the detection of Clavibacter michiganensis subsp. sepedonicus in cattle manure slurry

Various compounds and basal media were tested for their suitability to create a semi-selective medium for isolation ofClavibacter michiganensis subsp.sepedonicus (Cms) from cattle manure slurry containing c. 10⁸ colony forming units (cfu) per ml.Plating efficiency of Cms in yeast glucose mineral medium (YGM) was 104% compared with yeast peptone glucose medium. Nalidixic acid, polymyxin B sulphate and the experimental disinfectant S-0208 inhibited colony growth of cattle slurry bacteria as compared with Cms in YGM. The optimal concentration of these inhibitors in combination was determined by modified agar diffusion tests and by pour plating in 24-well tissue culture plates. The semi-selective medium YGMI consisted of YGM supplemented with nalidixic acid (2 mg/l), polymyxin B sulphate (30 mg/l) and S-0208 (125 mg/l). Plating efficiency varied for Cms between 50.9 and 69.6%, for cattle slurry bacteria between 1.8 and 2.5% and for saprophytes from potato heel end extracts between 11.5 and 27.4%.Differentiation of Cms colonies from other colonies was based on their small and bluish colony morphology in pour plates and on immunofluorescence colony-staining (IFC). IFC of a pure culture of micro colonies of Cms in YGM was possible after one day incubation (colonies c. 5 cells). Green background fluorescence in the agar gels was prevented by addition of Tween 20 (0.1%) to the washing buffer and the use of 1% agar gels. IFC of macro colonies of Cms in YGMI, visible with 4x objective magnification, was possible after 4 days. The detection level of the target organism in artificially inoculated cattle slurry in YGMI based on colony morphology varied between 1.4×10³ and 2.3×10⁴ cfu per ml of cattle slurry. Miniaturized plating combined with IFC, using wells in tissue culture plates (=16 mm), proved suitable for detection, but was c. 30 times les sensitive. The recovery of Cms was negatively correlated with the number of saprophytic colonies in the agar plates (R ²=0.74).     

光敏核不育水稻选育与利用的几个问题讨论

对现有光敏核不育材料进行了分类,分为４种遗传类型;其中,温光弱感到光敏核不育系是最为理 想的遗传工具,并就其选育途径和选择“粳不籼恢”繁殖制种技术体系进行了论证．     

“复激保果剂”在杂交水稻制种上应用技术总结

复激保累剂,在杂交稻制种上使用,能促使花器发育良好,调节父母本花期,提高结实率,增加每穗实粒和粒重,提高产量。且成本低,有显著的经济效益。     

Effect of diet composition and slaughter weight on animal performance, carcass and meat quality, and fatty acid composition in veal calves

The effect of true milk use in the diet of Spanish Brown Swiss male calves on animal performance, carcass and meat quality, and fatty acids composition was studied. In experiment 1, the effect of milk intake [ad libitum continuous (ADLIB) feed vs. restricted 0.7 during 75 days followed by ad libitum feed (RESTR)] and slaughter endpoint (225 kg vs. 5 month) were studied. In experiment 2, ad libitum concentrate feeding [grain-fed (GF)] was compared with milk supplementation until slaughter [milk-fed (MF)] in calves slaughtered at 345 kg. As regards to milk intake, carcass weight and degree of fatness were higher in the ADLIB group (P<0.05). The RESTR group revealed a higher percentage of saturated fatty acids (P<0.05). In experiment 2, the MF group exhibited a higher fat percentage (P<0.05), lower press and cooking losses (P<0.05), and higher scores for tenderness and juiciness (P<0.05) than the GF group. The percentage of saturated fatty acids was higher in the MF group (P<0.05). The results suggest that true milk use in veal production could be an advantageous alternative in terms on production costs, animal performance, and carcass and meat quality.     

饲粮中添加抗菌肽对肉鸭增重及血清尿素氮、总蛋白水平的影响

240只1日龄肉鸭随机成分4组,每组设3个重复,对照组用基础日粮,试验组在基础日粮上每千克分别添加1、2、3mL抗菌肽制剂。结果显示:1)2、3mL/kg添加量组增重效果显著(P<0.05),3mL/kg添加量组最佳;净肉率升高,达到显著水平(P<0.05),其中主要是胸肌率上升;腹脂率降低但差异不显著(P>0.05);料重比无显著变化(P>0.05)。2)血清总蛋白浓度差异不显著(P>0.05),但尿素氮浓度下降,2、3mL/kg添加量组达显著水平(P<0.05),3mL/kg添加量组最低。     

影响羊肉产量和品质的因素

本文综述了品种、饲料营养、饲养方式、性别、年龄、去势、屠宰等方面对羊肉产量和羊肉品质的影响,并提出建议。     

异黄酮植物雌激素在家禽生产中的作用

异黄酮植物雌激素广泛分布于植物界,其生理活性多种多样。它具有促进家禽生长,提高产蛋性能等作用,但对其作用机制目前尚未完全明确。本文综述了近年来有关异黄酮植物雌激素在家禽生产方面的研究概况。