体外模拟胃环境法优先排铅功能饮液配方的研制

以亚硒酸钠、葡萄糖酸钙、磷酸二氢钠、魔芋精粉为原料研制排铅功能饮液。以铅螯合率为指标,通过单因素和三因素三水平正交试验对饮液配比进行了分析,获得最佳的排铅饮液的配比为:亚硒酸钠的添加量为110μg·L-1,葡萄糖酸钙的添加量为7.5g·L-1,磷酸二氢钠的添加量为2.5g·L-1,魔芋精粉的添加量为0.4%。     

干式空气滤清器内部流阻特性仿真研究

基于流体动力学理论,对某干式空气滤清器空腔本体及含滤芯滤清器系统的内部流场进行了仿真分析,在台架上对其进出口压差进行了试验测量,并对仿真结果和试验测量结果进行了对比;通过仿真结果,深入了解了该空气滤清器内部的速度场、压力场等分布规律,得到了流阻特性随流量的变化关系,对以后空气滤清器的设计具有一定的指导意义.     

卵泡液中总蛋白含量与卵泡直径的关系

通过检测不同直径卵泡的卵泡液中总蛋白含量的变化,从而进一步阐明卵泡液中蛋白成分在卵泡发育过程中的作用。将直径为3~5、5~8、8~10mm的卵泡和囊肿卵泡(>21mm)分为4组(n=10),分别抽取卵泡液。通过TCA/丙酮方法进行样品处理;根据Bradford法检测原理,利用分光光度计对其进行蛋白定量研究。结果表明:3~5mm为4440μg/mL,5~8mm为976μg/mL,8~10mm为686μg/mL,囊肿卵泡(>21mm)为537.2μg/mL。经SPSS13.0软件分析,3~5mm卵泡液中蛋白平均浓度高于其他组(P<0.01);其他3组间差异均不显著(P>0.05);囊肿卵泡中蛋白含量最低。由此可得出,卵泡液中总蛋白浓度随着卵泡直径的增加而降低,表明卵泡液中的蛋白含量与卵泡发育的成熟度呈负相关。而囊肿卵泡的发生很可能和蛋白含量降低有关,为卵泡囊肿发病机制的研究提供新的理论参考。     

圆盘式磁流变离合器的磁路设计分析

分析了磁流变离合器的工作原理,针对一种圆盘式磁流变离合器模型,进行了离合器磁路的设计计算。应用Ansys软件进行了离合器的磁路建模和仿真分析,结果表明,设计的磁路满足要求,并拟合出了励磁电流与工作间隙中磁感应强度和离合器传递力矩的关系曲线,为进一步进行磁流变液离合器的结构设计提供参考。     

厌氧悬浮颗粒污泥床反应器流态和运行状态相关关系研究

第三代高效厌氧反应器是在第二代反应器基础上,主要对水力流态进行较大改进而产生的新型反应器,该类型反应器不仅具有第二代反应器生物量高的特点,同时又具有良好的水力混合特性,大大提高了反应器的处理效率。本文选择厌氧悬浮床反应器作为研究对象,考察了反应器在运行过程中运行状态与流态之间的关系,建立高效厌氧反应器水力流态模型,利用水力学流态模型的测定结果,讨论不同条件下反应器的短流、死区和膨胀率等影响。     

The influence of anticoagulants on the measurement of total protein concentration in equine peritoneal fluid

The aim of this study was to evaluate the influence of two commonly used anticoagulants (K3EDTA and lithium heparin) on refractometric and spectrophotometric measurement of total protein (TP) concentration in equine peritoneal fluid samples. The influence of a commercial solution of K3EDTA, a solution of K3EDTA in distilled water and lithium heparin on the refractometric and spectrophotometric (biuret) quantification of TP content in peritoneal fluid samples was assessed. Total protein concentration measured by refractometry was consistently overestimated in samples with commercial K3EDTA. The solution of K3EDTA in distilled water only caused TP overestimation at high K3EDTA concentrations (>5 micromol/ml). By contrast, lithium heparin did not influence the refractometric values of TP. Neither anticoagulant modified TP values when measured by the biuret method. In conclusion, the use of K3EDTA as anticoagulant may result in a significant overestimation of TP values of peritoneal fluid samples measured by refractometry.     

Neospora caninum associated with septic peritonitis in an adult dog

A 7-year-old, male neutered Rhodesian Ridgeback dog was referred to the University of California-Davis Veterinary Medical Teaching Hospital with a 4-month history of peritonitis and episodic abdominal discomfort, lethargy, and weakness. Marked abdominal distension with a prominent fluid wave was noted on physical examination. Cytologic analysis of the abdominal fluid indicated a septic exudate with mixed bacteria and many protozoal zoites. Differentials for the identity of the protozoal zoites included Toxoplasma gondii, Sarcocystis neurona, and Neospora caninum. Indirect latex agglutination antigen testing, standard indirect fluorescent antibody testing, and PCR analysis were performed to identify the zoites. The dog's serum antibody titer for N caninum tachyzoites was 1:20,480, known polysera to N caninum reacted against zoites in the abdominal fluid, and PCR analysis of the abdominal fluid was positive for the presence of a known gene of N caninum. Based on the morphologic, immunologic, and molecular findings, the zoites were identified as N caninum. It remains unclear how the tachyzoites gained access to the peritoneal cavity. To the authors' knowledge, there are no reports of free N caninum in abdominal fluid of any species.     

Cerebrospinal fluid analysis and magnetic resonance imaging in the diagnosis of neurologic disease in dogs: a retrospective study

BACKGROUND: Diagnosis of central nervous system (CNS) abnormalities in dogs can be challenging antemortem. Historically, cerebrospinal fluid (CSF) analysis has been used for routine diagnostic evaluation of animals with suspected neurologic disease; however, with increasing availability of magnetic resonance (MR) imaging, the need for concurrent CSF analysis may be questioned. OBJECTIVE: The purpose of this study was to retrospectively assess and compare the diagnostic information contributed from MR imaging and CSF analysis in a population of dogs presenting with neurologic disease. METHODS: Results of concurrent MR imaging and CSF analysis were evaluated in dogs presented for neurologic diseases. Based on clinical diagnosis, the sensitivity of CSF analysis and MR imaging for detecting a nervous system abnormality was calculated. Dogs with diagnoses confirmed by other diagnostic modalities were also evaluated separately. RESULTS: A total of 256 dogs were included in the study. For clinical diagnoses in which abnormalities were expected, MR imaging abnormalities were found in 89% and CSF abnormalities in 75% of dogs; CSF abnormalities were more common than MR imaging abnormalities only in inflammatory CNS disease. The majority of CSF abnormalities were nonspecific; an etiologic diagnosis was determined in only 2% of CSF samples. MR imaging excelled in detecting structural disorders, revealing 98% of vertebral abnormalities. In confirmed cases (n = 55), 76% of MR images and 9% of CSF samples were diagnostic. When intervertebral disk disease (IVDD) and vertebral malformation were excluded from analysis (n = 16 remaining), 25% of MR images and 6% of CSF cytology results were highly indicative of the confirmed diagnoses; CSF titer results provided the diagnosis in 25% of these cases. CONCLUSION: CSF analysis may not be necessary when MR findings of IVDD or vertebral malformation/instability are obvious; however, when the cause of neurologic disorder is uncertain, concurrent MR imaging and CSF analysis provides the greatest assistance in establishing a clinical diagnosis.