胶冻样类芽孢杆菌对不同施肥处理的马铃薯产量和品质的影响

以‘克新1号’马铃薯为试验材料,试验设置低、中、高3个施肥量处理,在复合肥和有机肥的基础上,每个处理中固体胶冻样类芽孢杆菌微生物菌剂设置4个不同的梯度处理,在马铃薯块茎膨大期冲施定量的液体胶冻样类芽孢杆菌微生物菌剂,探究增施胶冻样类芽孢杆菌微生物菌剂对马铃薯生长指标、产量、品质及经济效益等的影响,旨在找出适合蒲县当地的科学施肥方法,为其马铃薯的高产优质生产提供理论和生产实践依据。结果表明,适量增施微生物菌剂有利于提高马铃薯产量、品质和经济效益等指标,但高浓度的微生物菌剂却抑制马铃薯的生长,在本试验中以低肥量处理下的T2组效果最佳,其株高、出苗率、维生素C含量、产量及净利润比常规施肥组分别提高了6.83%、5.31%、92.11%、27.0%和16.94%,还原糖含量降低了18.42%,是高产优质马铃薯的科学施肥配方及标准化生产技术方案。     

Effects of remifentanil on monophasic action potential and transmural dispersion of repolarization in rabbit myocardium

AIM: To study the effect of remifentanil on monophasic action potential and transmural dispersion of repolarization (TDR) in the 3-layer myocardium of isolated rabbit hearts. METHODS: Adult rabbits (n=18, 2.0 ~ 2.5 kg) were used to isolate the hearts for preparing Langendorff perfusion model. The hearts were randomly divided into 3 groups after perfusion with K-H solution for 15 min: the perfusion in control group (C group) continued for 60 min; the hearts in remifentanil group (R group) were perfused with 12 μg/L remifentanil K-H solution for 60 min; the hearts in remifentanil+aminophylline group (RA group) were given 60-min perfusion of 12 μg/L K-H remifentanil+30 mg/L aminophylline. The HR and 3 layers of myocardial monophasic action potential (MAP) in the left ventricular anterior wall were recorded at time points after balanced infusion for 15 min (T₀), and continued perfusion for 15 min (T₁), 30 min (T₂) and 60 min (T₃). The monophasic action potential duration of repolarization at 90% (MAPD₉₀) and the transmural dispersion of repolarization (TDR) were calculated. The early afterdepolarization, delay afterdepolarization and arrhythmia were also observed. RESULTS: In R group, slower HR and prolonger MAPD₉₀ and TDR at T₁~T₃ were observed as compared with those at T₀ (P<0.05). R group showed slower HR and longer MAPD₉₀ and TDR than C group and RA group (P<0.05). CONCLUSION: Remifentanil slows the HR, extends the MAPD₉₀ and increases the TDR, thus being prone to induce reentry. Aminophylline makes HR faster and MAPD₉₀ shorter, thereby reducing the TDR.     

服务质量管理体系在大学图书馆中的应用

本文介绍了服务质量管理体体系及其在大学图书馆中的应用,以实例说明图书馆服务质量管理的内容,以及服务质量管理测评对图书馆产生的效益。     

Geostatistical interpolation and aggregation of crop growth model outputs

Many crop growth models require daily meteorological data. Consequently, model simulations can be obtained only at a limited number of locations, i.e. at weather stations with long-term records of daily data. To estimate the potential crop production at country level, we present in this study a geostatistical approach for spatial interpolation and aggregation of crop growth model outputs. As case study, we interpolated, simulated and aggregated crop growth model outputs of sorghum and millet in West-Africa. We used crop growth model outputs to calibrate a linear regression model using environmental covariates as predictors. The spatial regression residuals were investigated for spatial correlation. The linear regression model and the spatial correlation of residuals together were used to predict theoretical crop yield at all locations using kriging with external drift. A spatial standard deviation comes along with this prediction, indicating the uncertainty of the prediction. In combination with land use data and country borders, we summed the crop yield predictions to determine an area total. With spatial stochastic simulation, we estimated the uncertainty of that total production potential as well as the spatial cumulative distribution function. We compared our results with the prevailing agro-ecological Climate Zones approach used for spatial aggregation. Linear regression could explain up to 70% of the spatial variation of the yield. In three out of four cases the regression residuals showed spatial correlation. The potential crop production per country according to the Climate Zones approach was in all countries and cases except one within the 95% prediction interval as obtained after yield aggregation. We concluded that the geostatistical approach can estimate a country’s crop production, including a quantification of uncertainty. In addition, we stress the importance of the use of geostatistics to create tools for crop modelling scientists to explore relationships between yields and spatial environmental variables and to assist policy makers with tangible results on yield gaps at multiple levels of spatial aggregation.     

一份多年生高粱属新材料的农艺性状及其饲用潜力评价

为明确高粱属新材料‘SX-1’的基本性能,对其饲用潜力进行初步评价,本研究以‘晋牧1号’高丹草(Sorghum bicolor × S. sudanense cv. ‘Jinmu No. 1’)为对照进行连续2年品比试验,对其农艺性状、产量和营养成分等进行测定。新材料‘SX-1’表现为多年生且雄性不育,第1年抽雄期刈割时平均株高为311.33 cm,显著高于(P<0.05)‘晋牧1号’(263.00 cm),分蘖数为12.00~13.00个,第2年分蘖数极显著提高（可达40个以上）。建植第1年鲜草总产量为110822.43 kg/hm²,显著低于(P<0.05)‘晋牧1号’(125846.43 kg/hm²);但干草总产量(22300.71 kg/hm²)显著高于(P<0.05)‘晋牧1号’(19039.32 kg/hm²)。‘SX-1’建植第2年鲜、干草总产量为150373.33 kg/hm²和28866.77 kg/hm²,分别较‘晋牧1号’高10.53%和38.00%,同时较第1年增加了35.69%和29.44%。粗蛋白、中性洗涤纤维、粗脂肪含量年平均分别为9.98%、53.28%和2.20%,均与‘晋牧1号’(10.05%、53.93%、2.09%)差异不显著(P>0.05);酸性洗涤纤维年均为35.25%,显著低于(P<0.05)‘晋牧1号’(45.20%);粗灰分为8.59%,显著高于(P<0.05)‘晋牧1号’(7.57%)。相对饲用价值为107.27,高于‘晋牧1号’(92.61)。新材料‘SX-1’生物产量高、耐刈割、饲用品质优良,值得进一步开发利用。具有多年生高粱血缘的高粱属种间杂交种是选育饲草高粱的潜在方向。     

稻麦两熟制下秸秆还田模式的产量和经济效益分析

明确不同秸秆还田模式对小麦、水稻产量和经济效益的影响,对推广秸秆还田具有重要意义。利用稻麦复种长期田间定位试验2012-2018年的数据,分析了秸秆不还田（S0）、麦秸还田（WS）、稻秸还田（RS）和稻麦秸均还田（WRS）4种秸秆还田模式对作物产量、氮肥利用率、秸秆增产率、秸秆边际产量和秸秆农学利用率的影响。结果表明：秸秆还田均增加水稻产量,其中RS和WRS模式增产效果均显著大于WS模式;小麦产量随秸秆还田年限呈“减产-稳产-增产”的变化趋势。秸秆的周年增产率和农学利用率分别为5.65%~13.60%和0.11~0.17kg/kg。与RS模式相比,WRS模式属于秸秆过量还田,其秸秆增产效率降低。秸秆还田提高稻麦氮肥周年利用率1.67~4.01kg/kg,其中RS和WRS模式较WS模式更利于作物的氮吸收。WS、RS和WRS模式的稻田周年净收益较不还田模式分别增加1 690、4 875和4 177元/（hm²·年）,其中RS和WRS模式均显著高于WS模式。综合以上结果,麦季稻秸还田模式（RS）既可提高还田秸秆利用率,又可增加稻田净收益,推荐在稻麦两熟农田采用。     

Hesperetin attenuates hypoxia/reoxygenation-induced apoptosis in H9c2 cells via inhibition of calcium overload

AIM: To investigate the effect of hesperetin on hypoxia/reoxygenation (H/R)-induced apoptosis in the H9c2 cells and to clarify the underlying mechanism. METHODS: The H/R model was established and the H9c2 cells were pretreated with hesperetin for 4 h. The cell viability and cell damage were measured by CCK-8 assay and lactate dehydrogenase (LDH) detection. The apoptosis was analyzed by Hoechst 33258 staining and flow cytometry. The intracellular calcium fluorescence intensity was measured by fluorescence microscopy and flow cytometry. The calcium-ATPase activity and the level of adenosine triphosphate (ATP) were measured by ELISA. The mitochondrial membrane potential was measured by JC-1 staining. The protein expression levels of Bcl-2, Bax and cytochrome C (Cyt-C) were determined by Western blot. RESULTS: Hesperetin reduced the apoptosis of the H9c2 cells induced by H/R, decreased intracellular Ca²⁺ fluorescence intensity, elevated Ca²⁺-ATPase activity, inhibited the mitochondrial membrane potential depolarization and increased the level of ATP (P<0.05). In addition, hesperetin significantly reduced the release of Cyt-C protein from mitochondria to cytoplasma and increased the Bcl-2/Bax ratio (P<0.05). After using the calcium ion inhibitor nimodipine, the percentage of the cells with mitochondrial membrane depolarization was decreased, the ATP level was increased and the protein expression of mitochondrion-related apoptosis molecules were decreased (P<0.05). CONCLUSION: Hesperetin reduces the apoptosis of the H9c2 cells induced by H/R, which may be related to inhibition of calcium overload and improvement of mitochondrial function.     

河南长江流域防护林工程水土保持效益评价

涵养水源和保育土壤是森林的主要生态功能。通过采用森林生态系统服务功能实物量、价值量评估方法对长防林工程实施区域森林的水土保持效益进行的评价,结果表明,长防林工程实施后林地可调节水量12.936亿m3,固土0.135亿t,林地的水土保持效益价值达126.247亿元。经过20年的长防林工程建设,项目区内森林资源快速增长,水土流失面积大幅度减少,工程建设对生态环境的改善起到了明显的作用。     

新型烟草漂浮塑料育苗池的研发及效益分析

新型漂浮塑料育苗池是一款在烟苗育苗建池环节中环保、高效的育苗器具,该文总结漂浮塑料育苗池的研发过程,并与常规木板育苗池进行生产效益对比分析.     

Effect of Psammaplin A on in vitro Development of Bovine Aging Oocytes

To investigate the effect of histone deacetylation inhibitor Psammaplin A (PsA) on the development of bovine aging oocytes in vitro,oocytes were randomly divided into control group,aging group and 50 mmol/L PsA treated aging group (PsA group).Immunofluorescence staining and JC-1 were used to detect the blastocyst rate of bovine oocytes after parthenogenetic activation,the number of cells in blastocysts,apoptosis,reactive oxygen species (ROS),glutathione (GSH) and mitochondrial membrane potential intensity of embryos.The results showed that the blastocyst rate of the aging group was significantly lower than that of PsA and control groups (P<0.05).The blastocyst rate of PsA group was not significantly different from that of control group (P>0.05).The number of cells in the blastocysts of control group and PsA group were significantly higher than that of aging group (P<0.05).The number of cells in the blastocysts of PsA group was not significantly different from that of control group (P>0.05).The apoptosis rate in aging group was significantly higher than that of control and PsA groups (P<0.05),the apoptosis rate of PsA group was significantly higher than that of control group (P<0.05).The GSH level of MⅡ oocytes in aging group was significantly lower than that of control and PsA groups (P<0.05).There was no significant difference in GSH level between control and PsA groups (P>0.05).The ROS level of the embryos in aging group was significantly higher than that of control and PsA groups (P<0.05).The ROS level in PsA group was significantly higher than that of control group (P<0.05).The mitochondrial membrane potential of early embryos of aging group 4-8 cells was significantly lower than that of control and PsA groups (P<0.05).The mitochondrial membrane potential intensity of control group was significantly higher than that of PsA group (P<0.05).In summary,PsA could effectively delay the aging of bovine oocytes and improve the quality of oocytes.