Isolation and Identification of Cellulase-producing Bacillus in Yak Rumen

To investigate the species of cellulase-producing Bacillus in yak rumen,10 samples of rumen content were aseptically collected from 10 adult Maiwa yaks to isolate the heat-resistant Bacillus by water bath at 80 ℃ for 20 min.The cellulase-producing strains were screened using the CMC-Na medium and Congo red staining.The 16S rRNA gene sequence of those cellulose-producing strains were amplified and sequenced.The results showed that 64 strains were isolated from the 10 samples.Total 23 strains were identified as cellulase-producing bacillus,including 16 strains of Bacillus cereus,7 strains of Bacillus thuringiensis.Furthermore,phylogenetic analysis showed that the 16 Bacillus cereus strains were clustered into two branches:One isolate was clustered into a branch alone,the other 15 isolates were clustered into a branch which clustered into 5 small branches,showing that there was certain genetic diversity in the isolates of Bacillus cereus.And all 7 Bacillus thuringiensis strains were clustered into a branch.Hence,the results layed the foundation of investigating the species of cellulase-producing Bacillus in yak rumen and developing probiotics special for yak.     

Basic characterization of avian β‐defensin genes in the Japanese quail,Coturnix japonica

In this study, we identified a cluster of 14 avian β‐defensins (AvBD; approximately 66 kbp) in the Japanese quail, Coturnix japonica. Except for AvBD12 (CjAvBD12) and ‐13, the CjAvBDs coding sequences exhibited greater than 78.0% similarity to the respective orthologous chicken AvBD genes (GgAvBD). The putative amino acid sequence encoded by each CjAvBD contained six cysteine residues and the GXC (X1‐2) motif considered essential for the β‐defensin family. Each CjAvBDs also formed a sub‐group with the respective orthologous genes of various bird species in a phylogenetic tree analysis. Synteny between the CjAvBD cluster and GgAvBD cluster was confirmed. The CjAvBD cluster was mapped on the long‐arm end of chromosome 3 by linkage analysis based on single nucleotide polymorphisms (SNPs) of CjAvBD1 and CjAvBD12 (approximately 46kbp), as well as GgAvBD cluster. We also confirmed that CjAvBD1, ‐4, ‐5, ‐9, and ‐10 are transcribed in 20 tissues, including immune and digestive tissues. However, our experimental data indicated that the CjAvBD cluster lacks the AvBD3 and ‐7 loci, whereas the CjAvBD101α, ‐101β, and ‐101θ loci arose from gene duplication of the AvBD6 orthologous locus in the CjAvBD cluster after differentiation between Coturnix ‐ Gallus.     

Characterization,phylogenetic analyses and pathogenicity of Bipolaris setariae associated with brown stripe disease on sugarcane in Yunnan,China

Brown stripe disease is a severe foliar fungal disease of sugarcane worldwide and is widespread in all sugarcane planting areas in China. Brown stripe is a major disease that seriously affects the output and quality of the sugarcane industry in Yunnan Province, China's second-largest sugar base, while the pathogen of this disease remains not yet fully understood. To address this, we isolated and identified the fungi associated with 68 leaf samples showing typical symptoms of brown stripe from 22 sugarcane varieties in different areas of Yunnan Province. A total of 113 isolates were obtained, which were morphologically similar. Of these, 64 representative isolates were sequenced for the internal transcribed spacer region (ITS), GAPDH and EF-1α loci. All representative isolates grouped with the type strain of Bipolaris setariae in the phylogenetic trees inferred with individual and concatenated sequences of ITS, GAPDH and EF1-α. Pathogenicity test results showed that B. setariae strains were able to induce typical symptoms of brown stripe. The results obtained in this study clarify that only B. setariae is associated with sugarcane brown stripe in Yunnan, China. It is recorded here for the first time as a pathogen causing sugarcane brown stripe in Yunnan, and it is able to infect many major cultivars and new varieties, posing a new threat to the sugar industry in Yunnan Province. In addition, these results provide the scientific basis for the future breeding of disease-resistant varieties and effective prevention and control of sugarcane brown stripe disease.     

安徽药用牡丹炭疽病病原菌鉴定

<正>牡丹(Paeonia suffruticosa Andr.)是芍药科植物,为多年生落叶灌木,具有很高的观赏和药用价值。因其风姿华丽、色彩艳丽、品种繁多、深受大家喜爱,是中国传统名花和世界著名花卉之一。其根皮也被称为丹皮,具有抗炎、抑制血小板、止痛、清热等作用,是六味地黄丸等著名中成药的主要配方,也是饮片中的常用品[1-2]。近年来,随着牡丹种植面积的不断扩大,造成牡丹病害的大面积发生,已给牡丹生产带来严重影响。     

苹果树种植对黄土旱塬土壤蒸发的影响

以陕西长武旱塬为例,分别对研究区农地和5个不同林龄(9、12、16、19 a和23 a)苹果园的土壤剖面氢氧稳定同位素进行测定,利用Craig-Gordon模型定量估算其土壤平均蒸发量,并基于“空间换时间”方法分析果园种植及生长对土壤蒸发的影响。结果表明：农地及9、12、16、19、23 a林龄苹果园的土壤蒸发量均随苹果树的种植及生长呈现先减少再增大的趋势,年均蒸发量分别为129、104、89、119、128、136 mm;苹果园的土壤蒸发量变化与叶面积指数呈显著负相关(R=-0.713);苹果园种植的前中期(9～12 a)土壤蒸发量随叶面积指数增加逐渐减小,而在中后期(12～23 a)深层土壤水被大量消耗造成的干旱胁迫使得果树叶面积减少,从而导致林下土壤蒸发量又逐渐增大。     

Risk Decision-making Method of Slope Resistant Engineering

Considering the character of slope resistant engineering invest benefit, in line with security and economy balmaced, combining initial investment and anticipated hazard loss, disaster resistant engineering should be satisfied with the criterion of minimum cost. Decision tree model is adopted as analyzing means, and risk decision-making of slope design is carried out. Project input-output radio adopting dynamic analysis can reflect project investment benefit more accurately. At last this paper analyzes some questions in process of slope resistant engineering with benefits enumerate.     

图热点和树结点双向驱动汽车备件系统可视化研究

针对国内汽车企业不具备整车齐全的三维CAD模型的实际情况，提出一种基于动态装配图热点和产品结构树结点双向驱动的备件数据可视化管理方案，在Delphi开发平台上将Action Script技术和ActiveX技术集成到可视化备件管理系统中。详细阐述了图热点驱动和树结点驱动的程序实现流程．给出了动态装配图、装配结构树和界面数据窗三者之间的联动关系，实现两种驱动的等效性。为了便于用递归算法遍历结构树，对复杂网状结构的零部件从属依赖关系进行规范化处理．使之成为树形结构。采用约束递归迭代，对一次性加载零部件的层数和范围进行约束，用统一的虚拟临时结点代替当前非叶子结点的子结点，实现整车装配关系结构树构造过程的优化。     

单株幼龄苹果树修剪仿真研究

修剪是果树生产中重要的技术手段,要想研究更加合理的修剪技术体系,就必须保证能够对修剪反应有准确、直观和及时的判断,而仿真模拟技术提供了这种可能。以幼龄苹果树为研究对象,采用不同修剪处理对比的方法,在田间试验的基础上,总结修剪反应规律,构建苹果树结构和修剪模型。在此基础上基于Qt框架和OpenGL图形库,开发了单株苹果树修剪仿真软件平台。结合优化算法,通过仿真平台模拟了给定生产目标的修剪方案优化,优化结果对虚拟学习和实际生产提供了可视化指导。     

兰州南北两山集雨绿化生态水文变化机理初探

以兰州南北两山集雨绿化无灌溉人工生态系统为研究对象，在定位监测的基础上，运用生态水文学的基本原理，分析了拧条和柽柳土壤水分的土层分配、利用和水量平衡模式的变化，讨论了集雨区的植被盖度和结皮的发育成因和影响，以及拧条和柽柳在不同集雨面积的蒸散发量。得出在兰州南北两山集雨绿化可以推广乔木柽柳，其生态系统和生态水文过程比较稳定。