鸭ApoVLDL-Ⅱ基因第1内含子多态性及其遗传效应

以褐色菜鸭为材料,对其ApoVLDL-Ⅱ基因第1内含子进行PCR-SSCP检测,分析其多态性及遗传效应.结果表明,在ApoVLDL-Ⅱ基因第1内含子检测到多态性,存在BB、Bb和bb 3种基因型,序列比对发现存在T374C、C379T和T390G 3个单核苷酸多态位点,该位点属中度多态,并处于Hardy-Weinberg平衡状态(P>0.05).关联分析显示,bb基因型个体的蛋重极显著高于BB型(P<0.01),bb基因型个体的蛋白重显著高于BB型(P<0.05).推测该片段的多态位点可能对褐色菜鸭蛋重和蛋白重有一定的影响.     

安徽省知母药材质量研究

[目的]研究安徽产知母药材中知母皂苷BⅡ和芒果苷的含量.[方法]知母皂苷BⅡ采用Diamonsil C18柱（250 mm ×4.6 mm,5μm）、乙腈-水等度洗脱（25∶75）,流速1.0 ml/min、ELSD检测器漂移管温度60℃、载气流速3.0 ml/min、柱温为室温（25℃）;芒果苷采用Diamonsil C18柱（150 mm×4.6 mm,5μm）、乙腈-0.2％冰醋酸水溶液（15∶85）等度洗脱,流速1.0 ml/min.[结果]知母皂苷BⅡ在1.036～10.360 μg范围内（r=0.999 6）进样量的对数值与峰面积的对数值呈良好的线性关系,芒果苷在0.05 ～1.00 μg范围内（r=0.9994）进样量与峰面积呈良好的线性;知母皂苷BⅡ平均回收率为99.7％,RSD=1.19％;芒果苷平均回收率为100.3％,RSD=1.42％.[结论]安徽省知母药材中知母皂苷BⅡ与芒果苷含量均符合2010版药典含量标准.     

基于ARM9内核的智能灌溉控制系统的设计

介绍了基于ARM920T内核的嵌入式控制系统的设计与实施,并将控制系统引入设施栽培自动灌溉控制中。实践证明,该系统的设计达到了预期的效果。     

Physiological Responses of Wheat (Triticum Aestivum L.) –Aegilops Sharonensis Introgression Lines to Excess Copper

Two wheat lines (TL3 and TL5) derived from selection for Cu tolerance among wheat – Aegilops sharonensis hybrids were compared when grown as hydroponics at zero (control), 10⁻⁶ and 10⁻⁵  m CuSO₄. The morphometric measurements showed that the line TL5 with a more inhibited root system under 10⁻⁶  m CuSO₄ had a less inhibited shoot growth under 10⁻⁵  m CuSO₄ as compared to TL3. The decreased maximum efficiency of photosystem II and the more pronounced trend towards increased catalase activity suggested that despite the better shoot growth, the leaves of TL5 were more functionally injured. The increased content of carotenoids at excess Cu and the higher peroxidase and catalase activities of TL3 in control plants might contribute to its better stress tolerance. While no significant changes in enzyme activities were found at 10⁻⁶  m Cu, at 10⁻⁵  m the activities tended to increase. Although the close values of free phenolics concentrations in control plants, at Cu excess their content was higher in TL5 compared to TL3. The free phenolics content in roots at 10⁻⁶  m Cu decreased, and although higher at 10⁻⁵  m Cu, it remained below the control in TL3, and above the control in TL5. From the obtained results TL3 emerged to be more tolerant to excess Cu than TL5.     

反刍家畜主组织相容复合物的研究进展

MHC是由紧密连锁的高度多态的基因座位所组成的染色体上的一个遗传区域 ,由于在免疫系统中发挥着重要作用并与抗病性能密切相关 ,因此近年来成为家畜抗病育种研究中最为活跃的部分。本文综述了牛、绵羊和山羊MHC的结构、特点及其与抗病性状之间的关系 ,同时讨论了MHC在家畜育种中的应用前景。     

Protective effect of adiponectin on glucose and lipid metabolism by suppressing MHCⅡ expression

AIM: To investigate whether the protective effect of adiponectin on glucose and lipid metabolism is achieved through down-regulating major histocompatibility complex class Ⅱ (MHCⅡ) in the adipose tissue. METHODS: Adiponectin knockout (KO) mice and C57BL/6(WT) mice were fed with high-fat diet and standard diet for 24 weeks, respectively. The body weight, fasting blood glucose (FBG), fasting insulin (FINS), homeostasis model assessment of insulin resistance (HOMA-IR), triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), hepatic histology, and class Ⅱ trans-activator (CⅡTA), histocompatibility 2 class Ⅱ antigen E beta (H2-Eb1) and cluster of differentiation 74(CD74) mRNA and MHC Ⅱ protein levels in adipose tissue were measured at sacrifice. siRNA targeting MHC Ⅱ and overexpression vector was used in 3T3-L1 cells to explore the effect of adiponectin on the protein level of MHCⅡ. RESULTS: The levels of body weight, FBG, FINS, HOMA-IR, TC, TG, LDL-C, hepatic steatosis, CⅡTA, H2-Eb1 and CD74 mRNA expression, and MHCⅡ protein expression in the KO mice were higher than those in the WT mice that fed with high-fat diet or standard diet. In 3T3-L1 cells, inhibition of adiponectin reversed MHC Ⅱ protein level induced by specific siRNA. The expression of MHC Ⅱ in adipocytes decreased after adiponectin was overexpressed. CONCLUSION: Adiponectin improves glucose and lipid metabolism through suppressing the expression of MHCⅡ in the adipose tissue.     

玉米几个主要农艺性状的遗传研究

采用增广NCⅡ设计,研究玉米的株高、穗位高、穗长、穗粗、穗行数、粒长、百粒重和小区产量的遗传。结果表明,穗位高和百粒重符合加性-显性遗传模型,株高和穗行数除加性-显性效应外还有上位性效应,粒长和小区产量除加性效应外还有上位性效应,穗粗和穗长只有加性效应。显性性质:株高和穗位高平均为部分显性,百粒重和穗行数平均为超显性,显性方向株高、穗位高、百粒重和穗行数均为双向显性。     

玉米CMS-S恢复基因Rf3近等基因系的蛋白质分析

 【目的】Rf3是玉米CMS-S型不育系两个以上恢复基因中的标准基因，对其进行分子生物学研究意义很大。【方法】利用自主创建的Rf3近等基因系不育群体（Ps）和可育恢复系群体（Pf）为试材，采用改进的双向（二维）聚丙烯酰胺凝胶电泳（2D-PAGE）技术，对苗期、成株期叶片细胞总蛋白进行分析。【结果】分离到在Pf遗传背景下分子量为25.2 kD，等电点为5.0的特异蛋白质RRPⅡ，优化了2D-PAGE技术。【结论】该特异蛋白质点的出现与消失可能与玉米CMS-S的育性恢复有一定关系，对其纯化分离将为Rf3基因的克隆奠定基础和进一步了解玉米质核互作不育表达模式的分子机制。结合本研究，文章还讨论了采用2D-PAGE技术在玉米分子生物学研究中的优化以及基因组学与蛋白组学研究的衔接。     

质粒DNA 免疫小鼠制备IGF-Ⅱ抗体的研究

[目的]用基因免疫法制备人类胰岛素样生长因子Ⅱ(IGF-Ⅱ)的抗体,从而检验基因免疫法的可行性。[方法]通过RT-PCR法克隆到IGF-Ⅱ基因,将其连接到pcDNA3.1A载体上,用含IGF-Ⅱ基因的质粒通过皮下、肌肉注射2种途径免疫小鼠,制备抗血清;用Western blot法检测抗体特异性和抗体效价,探索基因免疫法制备抗体的方法,并进一步观察免疫次数及途径对抗体产生的影响。[结果]基因免疫不同次数的小鼠体内均有抗体产生,且皮下、肌肉不同免疫途径的血清均显示阳性结果,其中皮下注射免疫抗体产生较早;抗体效价可达到1∶500,而且特异性较强。[结论]通过基因免疫法制备IGF-Ⅱ抗体是可行的,而且效果较好。     

“防僵灵Ⅱ号”对家蚕真菌病的防治效果研究

[目的]有效控制家蚕真菌病的发生与蔓延。[方法]研究蚕药"防僵灵Ⅱ号"对家蚕真菌病的防治效果和对家蚕的生长发育、全茧量、茧层量的影响。[结果"]防僵灵Ⅱ号"在稀释500倍以上时对家蚕常见真菌病的防治效果达100%,对家蚕无明显毒副作用。[结论]"防僵灵Ⅱ号"对于家蚕真菌病具有良好的防治效果,同时是一种对家蚕安全的药剂。