棉花蕾期的栽培管理技术

棉株现蕾后即从营养生长进入生殖生长,但仍是营养生长占优势,以增大营养体为主。这一阶段的田间管理重点是在壮苗早发的基础上,采取合理的促控措施,调节棉株地上部和地下部、营养生长和生殖生长的关系,实现发棵稳长。发棵是高产的基础,稳长是高产的保证。     

当归优质高效栽培技术

介绍了当归育苗、施肥、定植、田间管理、病虫害防治等栽培技术,以指导当归大田生产.     

林下姜科植物的选育与栽培技术研究综述

树木褐根病是由有害木层孔菌 (Phellinus noxius) 引起的一类土壤传染性根部病害，为了实施对 这类病害的生物防治，结合唯一氮源 1- 氨基环丙烷 -1- 羧酸（ACC）为底物和平板对峙法，从澳门松山市 政公园健康树木的根际分离得到 1 株对有害木层孔菌具有较强拮抗活性的菌株 1583。采用一对细菌通用 引物 PCR 扩增获得菌株 1583 的 16S rDNA 基因序列，其片段大小为 1 496 bp，系统进化树显示菌株 1583 与 Burkholderia seminalis 的亲缘关系最近，综合结果鉴定该菌株为伯克霍尔德菌（Burkholderia sp.）。拮抗 性研究表明，菌株 1583 对油菜菌核病病原菌（Sclerotinia sclerotiorum）、黑松叶斑病病原菌（Pestalotiopsis sp.）、香蕉枯萎病病原菌（Fusarium oxysporum f. sp. cubense）、白蝴蝶炭疽病病原菌（Colletotrichum sp.） 和美人蕉瘟病病原菌（Pyricularia cannaecola hashioka）等植物病原真菌均有明显的抑制作用。     

论噬菌体在动物疾病治疗方面的应用研究

所谓噬菌体指的是真菌、放线菌、感染细菌等微生物的细菌病毒总称,它最为显著的特性是个体小、细胞结构不完整、仅有一个核酸。主要对噬菌体在动物疾病方面的应用情况进行了一番详细的论述。     

Detection of HCV core protein,envelope glycoprotein and core-envelope fusion protein by baculovirus surface display

AIM: To detect the hepatitis C virus (HCV) at early stages by the technique of baculovirus surface display. METHODS: We constructed the recombinant baculoviruses vAc-Flag-HCV C-GP64, vAc-Flag-HCV E-GP64 and vAc-Flag-CE-GP64, which displayed HCV-related structural proteins by the technique of baculovirus surface display. RESULTS: Western blotting results showed that the core (C), envelope (E) and core-envelope (CE) proteins of HCV were expressed in Sf9 cells after the infection of recombinant baculoviruses. We also observed by confocal microscopy that the C, E and CE proteins of HCV presented and were anchored on the plasma membrane of Sf9 cells after infection. In addition, the results of immunogold electron microscopy demonstrated that the 3 recombinant baculoviruses displayed the C, E and CE proteins of HCV on the viral surface, respectively. To further define the role of these recombinant baculoviruses in detecting HCV at early stages, we applied time-resolved fluoroimmunoassay (TRFIA) to detect HCV samples after purification and concentration of the recombinant baculoviruses. The results showed that the positive rate of vAc-Flag-CE-GP64 was up to 80.2%. CONCLUSION: vAc-Flag-CE-GP64 is useful for detecting the hepatitis C virus at early stages.     

甬优9号在三明市的种植表现及关键栽培技术

甬优9号在三明市试种表现高产稳产、产量潜力大。该文总结了甬优9号在三明市的种植表现,并提出高优关键栽培技术。     

鸡山农场天然橡胶新品种抗性试验

高产优质抗病甘薯新品种湛薯407是广东省湛江市农业科学研究所以龙薯1号为母本、鸡母薯为父本，通过品种间杂交、多年多点综合鉴定选育而成。2008～2009年参加广东省甘薯新品种区域试验，2010年进行大田生产试验，各试验综合性状表现较好。该品种于2011年5月通过广东省农作物新品种鉴定，鉴定编号为粤审薯2011004。对湛薯407的品质性状、抗病性、产量等进行研究，结果表明：湛薯407薯干品质和食味较好，中抗薯瘟病，鲜薯和薯干产量与广薯111相比增产极显著，适应性广，适宜广东省各薯区种植。根据该品种的生长特性，还提供配套的栽培技术。     

浅析计算机网络安全

计算机网络技术应用越来越广泛，网络的安全性和可靠性也已成为不同使用层次用户共同关心的问题。本文从森工企业网络应用的现状和管理方面就网络安全的重要性、理论基础、具备功能以及解决措施等方面进行了简单阐述，并强调指出森工企业在网络安全方面应增强防范意识。     

印楝采种育苗及造林技术初步研究

对印楝Y.L07种源在元江试验区采种、育苗及造林技术研究结果表明：7月下旬至9月上旬,印楝果皮由青绿色变为黄色时是最佳采种期;自然状态下,种子发芽力随贮存时间的延长而递减;容器苗造林利于成活;苗木定植时可留干25cm进行截干处理。     

A New Cell Wall Located N-rich Protein is Strongly Induced During the Hypersensitive Response in Glycine Max L.

Soybean (Glycine max (L.) Merill, cv. Williams 82) plants and cell cultures respond to avirulent pathogens with a hypersensitive reaction. After inoculation of soybean with Pseudomonas syringae pv. glycinea, carrying the avirulence gene avrA, or zoospores from the fungus Phytophthora sojae Race 1, a resistance-gene-dependent cell death programme is activated. A new gene was identified by differential display of mRNAs that is specifically activated during the early phase of incompatible pathogen-soybean interactions but does not respond to compatible pathogens. The gene is strongly induced within 2h after addition of P. sojae zoospores. A similar kinetic pattern was observed for P. syringae (avrA) inoculated soybean cell cultures. The gene encodes a deduced protein of 368 amino acids with a very high content of asparagine and was therefore termed N-rich protein (NRP). The protein is composed of two distinct domains, of which only the C-terminal domain has striking homology to proteins of unknown function from other plants. An antibody raised against the recombinant NRP recognizes a protein of 42kDa. The protein is located in the cell wall as indicated by cell fractionation studies. Comparison of the genomic DNA-sequence with the cDNA, identified two introns within the open reading frame. The NRP-gene is not directly induced by salicylic acid or hydrogen peroxide, indicating a distinct and specific signal transduction pathway which is only activated during programmed cell death. The NRP-gene appears to be a new marker in soybean activated early in plant disease resistance.