低聚木糖的制备及其对益生菌体外增殖的作用

针对玉米芯微波消解-内切木聚糖酶水解制备低聚木糖的工艺,以低聚木糖的得率为主体评价指标,通过单因素实验时影响低聚木糖得率的微波消解过程和内切木聚糖酶水解过程的因素与水平进行研究,并考察获得的低聚木糖对益生菌的体外增殖作用.结果表明:玉米芯酶法制备低聚木糖的最佳工艺条件为微波处理压力1.6 MPa,微波处理时间5 min,内切木聚糖酶用量140 U·g-1,酶解时间6 h;在最适条件下,玉米芯酶解液中低聚木糖的得率为82.5%,质量浓度为11.02 g·L-1;低聚木糖对益生菌的体外增殖实验表明,低聚木糖添加量在0.2%和0.4%时,分别可以对乳酸杆菌和枯草芽孢杆菌起到最好的增殖作用,分别达到311%和183%,添加量进一步提高反而会抑制这2种菌的生长.     

Improve Water and Nutrient Efficiency in Tomato Crop by a Dynamic Fertigation Management under Saline Conditions

The improvement of water and nutrient efficiency leads to a production model that is more sustainable with less water, fewer fertilizer inputs, and less environmental damages. High-technology fertigation equipment permits high precision in the nutrient solution application. Besides, the field measurement of soil water content by tensiometers and the extraction of soil solutions by suction cups allow a dynamic methodology management in agreement with real crop requirements. This trial was carried out to compare this dynamic fertigation management method (using tensiometers and suction cups) for tomato crops (Lycopersicum sculentum Mill. Forteza) under Mediterranean greenhouse conditions with other methods: the local traditional model, based only on technical consulting, and the classical model, by means of estimation of Kc and nutrient extractions references. The parameters studied were tomato yield, water, and fertilizer amounts applied during the cultivation as well as water- and fertilizer-use efficiency. The water used to prepare the nutrient solution was classified as C₄-S₃ following the Riverside classification system. Plants were grown from 15 August to 20 April. The results show that the supply of fertilizers during the cultivation has been significantly lower with classical and dynamic models. Dynamic method shows greater efficiencies for all the elements, except for potassium, and also decreases the water consumption, not affecting total yield.     

不同地被物处理方式对红壤坡地养分状况的影响

选择3种不同水保措施的径流小区,即百喜草(Paspalum natatu)全园覆盖处理、全园敷盖处理和全园裸露处理,分析和测定了不同坡位和深度土层中土壤有机质、全N和全P空间异质性之间的关系。结果表明:(1)有机质含量随着土层增加整体呈先增加后减少的趋势,在自上而下坡面上,有机质含量全园覆盖区无明显变化,全园敷盖区有递增趋势,全园裸露区则先增加后减少;(2)全N含量随着深度加深均呈下降趋势,各处理间无显著性差异。在坡面上,全N含量大小为:全园覆盖区全园敷盖区全园裸露区,且全园敷盖处理在坡面中部有富集作用,全园覆盖和全园裸露处理自上而下呈下降趋势;(3)全P含量随着土层加深,全园覆盖区逐渐减少,全园敷盖区和全园裸露区先增加后减少。在坡面上,全P含量大小为:全园敷盖区全园覆盖区全园裸露区,且在坡面中部有富集作用。该研究可为亚热带地区红壤坡地养分恢复途径提供科学依据。     

微波消解-FAAS测定番木瓜不同部位的金属元素含量

为探讨番木瓜果肉、果皮、瓜籽中金属元素的含量,采用微波消解-FAAS法测定了番木瓜3个部位的K、Na、Ca、Mg、Cu、Fe、Zn共7种金属元素的含量,并对微波消解条件进行了优化。结果显示,在优化的微波消解条件下,样品消解完全、速度快、效果好,FAAS测定的各金属元素质量浓度与吸光度均呈良好的线性关系,相关系数r为0.995 7～0.999 9,检出限为0.002～0.131,回收率为98.30%～101.67%,RSD为0.20%～4.12%(n=6),说明测定方法可靠,结果稳定;番木瓜3个部位均含有较高的K、Ca、Mg,但Cu、Fe、Zn含量均较低,与番木瓜果肉相比,番木瓜果皮和瓜籽中K、Mg、Cu、Zn、Fe含量较高,食品和药品开发优势更突出。     

Effect of Integrated Nutrient Management on Rice Yield,Soil Nutrient Profile,and Cyanobacterial Nitrogenase Activity under Rice–Wheat Cropping System

We assessed the cyanobacterial inoculation, green manure (GM) application, and chemical nitrogen (N) fertilization on grain/straw yield, nutrient balance, and nitrogenase activity under individual and integrated nutrient management mode in a rice–wheat cropping sequence. Individual and integrated application of cyanobacterial biofertilizer (CB) and GM with chemical fertilizer improved the soil health and production of rice crop. Integration of cyanobacterial and green manure resulted in a savings of 50 kg N ha^?1. Functional relationships (R2, –83.5 to 95.7%) between the different sources of nutrients revealed that the maximum positive contribution of cyanobacteria was on final available N (45.2%) and available phosphorus (P, 18.5%). Green manure had the greatest contribution to total N, total P, zinc, iron, and manganese (Mn). However, cyanobacteria had a negative relationship with Mn and sodium (Na, –30.19%). A negative relationship with Na indicates the possibility of using cyanobacteria as an ameliorating agent for salt-affected soil.     

Sulfur content of plant material: A comparison of methods of oxidation prior to determination

Abstract

Procedures for S determination involving digestion of plant material with HNO₃/HClO₄ mixtures gave lower values than those using oxygen flask combustion. Tests on a range of S compounds showed that the HNO₃/HClO₄ procedure underestimated S present in methionine, cysteine and glutathione but S in sulfate and the glucosinolate, sinigrin, was not underestimated.

Low recoveries using HNO₃/HClO₄ digestion procedures can be due both to incomplete oxidation of certain compounds and to gaseous losses during the vigorous boiling stage of digestion.     

Calcium nutrition of the sugarbeet

Abstract

When sugarbeet seedlings are transferred from a complete nutrient solution to one from which Ca has been withheld, the rootlets and tops fail to develop. The same transfer at the eight‐leaf stage causes the rootlets to become stubby and swollen at the tips and blade expansion becomes modified; particularly the upper portions of the blades attaining nearly full development, which pucker and often develop a cupping or hooding effect; a unique symptom characteristic of Ca deficiency. As each new leaf develops, the blade area becomes smaller until only a black tip remains at the apex of the petiole, which is the symptom referred to as tip‐burn for this petiole and the successively . shorter petioles formed as Ca deficiency increases in severity. Strangely, these symptoms also appear during periods of rapid growth when the nutrient solution contains as much as 10 to 28 milliequivalents per liter of Ca or when soils are high in Ca. This implies that Ca absorption and possibly translocation limits the Ca supply at the growing point. Increasing Mg in the nutrient solution decreases Ca uptake and increases Ca deficiency. Potassium deficiency, unexpectedly, induces Ca deficiency apparently by decreasing the translocation of Ca to the growing point.

These phenomena suggest the hypothesis that when ion absorption takes place from the root exchange site that has the affinity for H > Ca > Mg > K > Na, then the H generated internally replaces, and the roots absorb, Na, K and Mg preferentially. Externally, Ca would be adsorbed preferentially from the nutrient solution by the exchange complex, and with the addition of Mg, it would compete for the common adsorption site of Ca and limit Ca absorption internally. Under these conditions potassium‐deficient nutrient solutions would not induce Ca deficiency by decreasing Ca absorption but rather by decreasing Ca translocation. Theoretically, Ba would replace H more readily than Ca on the exchange complex, and therefore, Ba would be adsorbed preferentially and Ca uptake would increase. This effect of Ba was verified experimentally.

Since the translocation of ⁴⁵Ca to the growing point was found to be unrestricted under Ca‐sufficient and Ca‐deficient conditions and since the formation of insoluble Ca compounds such as phosphate or oxalate did not account for the Ca deficiency at the growing point, the cause of the Ca deficiency at the growing point is most likely the higher priority of the storage root for Ca over tops when leaf blades and storage root are both expanding rapidly. However, Ca retransport from older to younger parts of the sugarbeet plant may be restricted by the formation of Ca phosphate under Ca‐deficient conditions and Ca oxalate under Ca‐sufficient conditions.

Calcium deficiency increases net photosynthesis per unit blade area initially, probably because of blade puckering, but not on a per unit chlorophyll basis.     

28％苄嘧磺隆·二氯喹啉酸WP对水稻秧田杂草及产量的影响

研究了28％苄嘧磺隆·二氯喹啉酸对水稻秧田稗草[Echinochloa crusgalli (L.) Beauv.]、鸭舌草[Monochoia vaginalis(Burm.f.)]等的防除效果,并测定了杂草对氮、磷、钾和水分的影响以及对水稻产量的影响.结果表明,28％苄嘧磺隆·二氯喹啉酸WP对水稻秧田的主要杂草具有良好的防治效果,有效成分用量为168～420 g/hm2的28％苄嘧磺隆·二氯喹啉酸对水稻秧田稗草密度防效和鲜重防效分别为84.06％～98.50％、91.55％～98.63％,对鸭舌草的密度防效和鲜重防效分别为94.06％～100.00％、90.94％～100.00％,显著优于对照药剂10％苄嘧磺隆WP和50％二氯喹啉酸WP.28％苄嘧磺隆·二氯喹啉酸施用后,增产效果总体优于对照药剂,在水稻秧田具有良好的推广前景.     

Effects of bark mulch and npk fertilizer on yield,leaf nutrient status and soil mineral nitrogen during three years of strawberry production

The effects of bark mulch and NPK fertilizers on yield and leaf and soil nutrient status of ‘Korona’ strawberry plants (Fragaria×ananassa Duch.), were studied over a period of three years. A significant effect of mulching was found in the first harvest year, but additional fertilizer did not affect total yield. Bark mulch slightly decreased the level of leaf nitrogen, but increased the level of leaf phosphorus and potassium in all years. Bark had a significant, negative effect on soil nitrate and ammonium content in the two first seasons. Mulching increased the soil moisture content in all years.