Effect of a size-based selection program on blood metabolites and immune response of Litopenaeus vannamei juveniles fed different dietary carbohydrate levels

Blood metabolites in wild and seventh-generation cultivated shrimp were measured to determine how size-based selection could alter the nutritional and immunological conditions of Litopenaeus vannamei. Wild L. vannamei juveniles and a sample of seventh-generation cultured shrimp were acclimated under identical conditions. During 55 days, shrimp were fed a high (HCHO: 44%) or a low (LCHO: 3%) carbohydrate diet for 55 days. Wild shrimp showed a direct relation between dietary CHO and lactate, protein and hemocyte levels indicating that dietary CHO was used for protein synthesis via transamination pathways. In seventh-generation cultured shrimp these parameters were inversely proportional to dietary CHO level, indicating the capacity to synthesize protein from dietary CHO was repressed in cultured shrimp. Farmed shrimp showed a limited capacity to respond to LCHO diets demonstrating high protein dependence in their metabolism and immune response. These results demonstrate that during size-based breeding programs other metabolic process than CHO catabolism can be selected. The incapacity of shrimp to use dietary CHO could limit protein reduction of diets and limit the efforts of the shrimp industry to be ecologically and environmentally profitable.     

Effects of the protein/carbohydrate ratio of extruded diets on protein synthesis, protein growth and body composition in juvenile brown trout (Salmo trutta)

High levels of protein in fish feeds result in higher costs and nitrogen waste. Therefore, studies focused on protein substitution by alternative energy sources are necessary. Here we examine whether the protein:carbohydrate ratio in extruded diets affects protein-turnover parameters and the main tissue components in brown trout. Juvenile fish were adapted to two extruded diets: one higher in protein and lower in carbohydrate, C: 45/28 and the other higher in carbohydrate and lower in protein, HC: 37/40. Gross energy was 19 MJ kg⁻¹ DM in both diets. Protein and lipid digestibility were high and similar in both groups (≈90%), whereas total and carbohydrate digestibility and digestible energy were lower in the HC-fed group than in the C-fed group. Consequently, when fish were adapted to the diets, plasma levels of glucose and insulin differed between diets. HC-group presented higher plasma glucose levels and lower plasma insulin levels than C-group. Protein synthesis rates in white muscle, liver and whole body did not differ significantly between diets. In contrast, protein accretion rate of white muscle and whole body were significantly lower in the HC group, indicating an increase of protein degradation in these tissues and a decrease of synthesis retention efficiency. In spite of the daily protein intake of HC group was lower than C-group, the anabolic stimulation efficiency was increased by 34% in HC group. Protein and lipid contents in white muscle and liver were stable throughout the experiment. No hepatomegalia or increased fat deposition was observed in fish fed HC. Differences in specific growth rates (C: 0.88%; HC: 0.77%) were associated more with the lower protein consumption rate and the lower level of digestible energy in fish fed HC than with the higher dietary carbohydrate content of the diet.     

Long-term nutritional effects on the primary liver and kidney metabolism in rainbow trout (Oncorhynchus mykiss). II. Adaptive response of glucose 6-phosphate dehydrogenase activity to high-carbohydrate/low-protein and high-fat/non-carbohydrate diets

The influence of long-term administration of high-carbohydrate/low-protein and high-fat/non-carbohydrate diets were studied in relation to kinetic behaviour of glucose 6-phosphate dehydrogenase in liver and kidney of rainbow trout. In all cases studied, the saturation curves of these enzyme showed typical hyperbolic kinetics without evidence of sigmoidicity. After 30 days of feeding with a high-fat diet (170 g kg^?1), there was a significant decrease in V_max and specific activity (45%) as well as catalytic efficiency (39%) without changes in K_m or activity ratio of hepatic glucose 6-phosphate dehydrogenase. These changes agree more with a clearly decreased cell concentration than with an inhibition of the pre-existing enzyme. The administration of a high-carbohydrate diet (60 g kg^?1), contrary to what was previously thought, decreased V_max by 21% and specific activity and catalytic efficiency by 30%, without significant changes in the other kinetic parameters of the hepatic enzyme. The kinetic behaviour under these nutritional conditons was due to the rejection of this diet by the fish and thus could be considered a low-feeding situation. On the other hand, no variations in the kinetics of renal glucose 6-phosphate dehydrogenase were found, clearly demonstrating that in this organ, the pentosephosphate pathway showed no adaptive response related to fattyacid and other lipid synthesis. The activity of the renal enzyme was consistently half that of the hepatic enzyme.     

钾硅肥配施对胡麻茎秆碳水化合物及抗倒性的影响

为明确钾硅肥施用对胡麻茎秆碳水化合物主要成分含量及抗倒伏特性的影响,探讨钾硅肥运筹调控胡麻茎秆抗倒伏能力的机制,选用裂区试验设计进行了研究,以两个品种为主处理,三个K2O钾肥用量(K0:不施钾;K1:52.5 kg/hm2;K2:105 kg/hm2)为副处理,两个SiO2硅肥用量(Si0:不施硅;Si1:90 kg/...     

Storage and Remobilization of Soluble Carbohydrates after Heading in Different Plant Parts of a Winter Wheat Cultivar

Abstract

Several types of water soluble carbohydrates (WSG) were traced into plant parts of a winter wheat (Triticum aestivum L.) cultivar from heading to physiological maturity. Field grown plants were harvested at intervals of a few days and divided into grain, chaff, leaf blades, leaf sheaths, rachis + peduncle and culm internodes. Leaf blades and sheath showed the peak of WSC contents at about anthesis. Culm internodes accumulated fructan and sucrose during the early grain-filling phase, from a week after anthesis until the milk-ripe stage, then remobilized them during late and final grain filling phases, from the milk-ripe stage until maturity. The amount of sucrose known as short-term storage WSC was higher than fructan known as long-term storage WSC in each internode throughout the grain-filling phase. Chaff showed a large amount of fructan and fructose before anthesis, although it did very little sucrose. A pattern of sucrose accumulation in chaff was very different from that of fructan, unlike the other parts. These patterns of changes in WSC contents in plant organs roughly corresponded with four grain-filling phases, the initial, early, late and final phases.     

Potassium deficiency impedes elevated carbon dioxide‐induced biomass enhancement in well watered or drought‐stressed bread wheat

Potassium (K) deficiency reduces photosynthesis and biomass production of crop plants and also renders them vulnerable to drought stress, whereas elevated carbon dioxide (CO₂) has a positive effect on photosynthesis and yield and ameliorates the adverse effects of drought stress. This study aimed to characterize the physiological responses of wheat (Triticum aestivum L.) stressed with K deficiency under elevated CO₂ and drought conditions. Increased biomass production caused by elevated CO₂ as a consequence of increased photosynthesis and water use efficiency was absent in young K‐deficient wheat plants. Shoot K concentration was negatively affected by elevated CO₂ particularly under K‐deficient conditions, whereas K content per plant was greatest in plants supplied with adequate K and adequate water. Specific leaf weight was increased as a consequence of carbohydrate accumulation in the source leaves of K‐deficient plants particularly under elevated CO₂ and drought stress. Potassium deficiency clearly impeded the impact of elevated CO₂ in both well watered as well as drought‐stressed plants. Adequate K fertilization is a prerequisite for efficient harvesting of atmospheric CO₂ through increased photosynthesis, decreased transpiration, and increased biomass production under changing atmospheric CO₂ and soil moisture conditions.     

化学处理对菌糠结构性碳水化合物瘤胃降解率的影响

选用 4头装有永久瘤胃瘘管的杂交肉用黄牛 ,采用 4× 4拉丁方设计研究化学处理对菌糠结构性碳水化合物瘤胃降解特性的影响。结果表明 :菌糖经碱化、氨化及氨化 +碱化处理后 ,NDF的动态降解率 ( P)和瘤胃潜在降解部分 ( a+ b)均有不同程度的提高 ,其中以碱化和氨化 +碱化处理效果最好。香菇菌糠经碱化和氨化 +碱化处理后 ,NDF、CEL及 HC的动态降解率 ( P)分别提高 36.30 % ( p<0 .0 5 )、2 0 .43% ( p<0 .0 5 )、1 1 3.1 1 % ( p<0 .0 5 )和 49.2 2 % ( p<0 .0 5 )、1 8.88% ( p<0 .0 5 )、1 1 0 .5 7% ( p<0 .0 5 )。     

Remobilization of Nitrogen and Carbohydrate from Stems of Bread Wheat in Response to Heat Stress during Grain Filling

When wheat (Triticum aestivum L.) is grown under heat-stress conditions during grain filling, preanthesis stored total non-structural carbohydrates (TNC) and nitrogen (N) could serve as alternative source of assimilates. This study was performed to evaluate wheat genotypes for their ability to accumulate and remobilize TNC and N stored in their stem to support grain filling under heat stress. Eighteen genotypes were used for N remobilization study while nine of them were used for TNC remobilization study. They were grown in pots and placed in a vinyl house with the maximum temperature kept below 30 °C. Five days after anthesis (5DAA), half of the pots were taken to phytotrons where temperature was gradually increased and the maximum was set at 38 °C. Grain yield and grain weight decreased by about 35 % under heat stress. Significant differences were found among genotypes in percentage reduction in grain yield, grain weight, grain filling duration and harvest index because of heat stress. The N and TNC concentrations of the stem at 5DAA were significantly different among genotypes. Heat stress significantly reduced the N remobilization efficiency of most of genotypes. However, heat stress significantly increased TNC remobilization efficiency and significant variation were observed among genotypes. N remobilization efficiency across treatments significantly correlated with grain yield, grain weight, harvest index and grain filling duration. TNC at 5DAA negatively correlated with N at 5DAA and harvest index, but the TNC remobilization efficiency under heat stress positively correlated with mainstem grain yield, grain weight and harvest index. The rate of chlorophyll loss from flag leaf positively correlated with N and TNC remobilization efficiencies under heat stress suggesting a link between leaf senescence and remobilization efficiency. The results indicate that heat stress negatively affected grain yield, its components and N remobilization while it increased TNC remobilization because of the increasing demand for resources.     

An Assessment of Variation for Nutritional and Non-nutritional Carbohydrates in Lentil Seeds (Lens culinaris)

The levels of the raffinose family of α-galactosides (raffinose, stachyose, verbascose and ciceritol) and of the nutritional carbohydrates (fructose, sucrose and starch) were determined in the seed of a range of lentil lines from germplasm collections and commercial Spanish cultivars. Significant levels of variation were found for all the measured characters, and particularly for the α-galactosides. The greatest variation was found in verbascose content, which ranged from about 1 % of the seed dry weight to undetectable amounts. The relationship between these chemical components and morphological seed characteristics was assessed. The only significant relationship between the different groups of characteristics was a positive correlation between the level of verbascose and the average seed weight for each genotype.     

Relationship between Frost Tolerance and Cold-Induced Resistance of Spring Barley, Meadow Fescue and Winter Oilseed Rape to Fungal Pathogens

Plants exposed to one stress factor may become more tolerant to another. Cold is the most often documented factor inducing plant resistance to pathogens. The aim of this work was to investigate whether resistance of spring barley and meadow fescue to Bipolaris sorokiniana and resistance of winter oilseed rape to Phoma lingam induced at 5 °C for 2, 4 or 6 weeks are associated with frost tolerance, water potential and soluble carbohydrate content. Cold‐acclimated plants of each species showed increased resistance to the studied pathogens. Barley, fescue and rape plants demonstrated higher frost tolerance after hardening, but only in the case of fescue a correlation between resistance to frost and resistance to B. sorokiniana was found. A significant decrease in the water potential of leaf cells was observed in cold‐acclimated barley and fescue. In these two species, water potential greatly affected resistance to B. sorokiniana. However, only in barley did accumulation of fructose, glucose and sucrose correlate as well with changes in water potential as with cold‐induced resistance to the pathogen. In the case of hardened rape, no correlation between the studied parameters was found. The results obtained indicated that the temperature of 5 °C used during cold acclimation was not favourable for hardening of this plant species.