Influence of selenium on oxidoreductive enzymes activity in soil and in plants

The aim of this greenhouse experiment was the assessment of the influence of H₂SeO₃ at soil concentrations of 0.05, 0.15 and 0.45 mmol kg⁻¹, on the activity of selected oxidoreductive enzymes in wheat (Triticum aestivum). The wheat plants were grown in 2 dm³ pots filled with dust-silt black soil of pH 7.7. Applied H₂SeO₃ caused activation of plant nitrate reductase at all concentrations, but activation of plant polyphenol oxidase at only two lower concentrations. The highest concentration caused inhibition of polyphenol oxidase and peroxidase. Plant catalase activity decreased under the influence of 0.15 and 0.45 mmol kg⁻¹ concentration. After the final analysis Se was quantified in plants and soil. The amounts in plants were: control (unamended soil) 1.95 mg kg⁻¹; I dose (0.05 mmol kg⁻¹) 18.27 mg kg⁻¹; II dose (0.15 mmol kg⁻¹) 33.20 mg kg⁻¹ and III dose (0.45 mmol kg⁻¹) 38.37 mg kg⁻¹, in soil: 0.265 mg kg⁻¹; 3.61 mg kg⁻¹; 10.53 mg kg⁻¹; 30.53 mg kg⁻¹; respectively. Simultaneously, a laboratory experiment was performed, where the activity of soil catalase and peroxidase were tested after 1, 3, 7, 14, 28, 56, and 112 days after Se treatment. Peroxidase activity in soil decreased with increasing Se content, over the whole experiment. The lowest dose of Se caused activation a significant 10% increase in catalase activity, but the influence of others doses was unclear.     

增硝营养对水稻不同生育时期生长及氮素吸收同化的影响

The effect of nitrate （NO3^-） on rice （Oryza sativa L.） growth as well as N absorption and assimilation during different growth stages was examined using three typical rice cultivars. Dry weight, yield, N uptake, nitrate reductase activity （NRA） in leaves, and glutamine synthetase activity （GSA） in roots and leaves during their entire growth periods, as well as the kinetic parameters of ammonium （NH4^＋） uptake at the seedling stage, were measured with solution culture experiments. Results indicated that addition of NH4^＋-N and NO3^-N at a ratio of 75：25 （NH4^＋＋NO3^- treatment） when compared with that of NH4^＋-N alone （NH4^＋ treatment） increased the dry weight of ‘Nanguang＇ cultivar by 30% and ‘Yunjing 38＇ cultivar by 31%, and also increased their grain yield by 21% and 17%, respectively. For the four growth stages, the total N accumulation in plants increased by an average of 36% for ‘Nanguang＇ and 31% for ‘Yunjing 38＇, whereas the increasing effect of NO3^- in the ‘4007＇ cultivar was only found at the seedling stage, in the NH4^＋＋NO3^- treatment compared to the NH4^＋ treatment, NRA in the leaves increased by 2.09 folds, and GSA increased by 92% in the roots and 52% in the leaves of the three cultivars. NO3^- supply increased the maximum uptake rate （Vmax） in the ‘Nanguang＇ and ‘Yunjing 38＇ cultivars, reflecting that the NO3^- itself, not the increasing N concentration, increased the uptake rate of NH4^＋ by rice. There was no effect on the apparent Michaelis-Menten constant （Kin） of the three cultivars. Thus, some replacement of NH4^＋ with NO3^-could greatly improve the growth of rice plants, mainly on account of the increased uptake of NH4^＋ promoted by NO3^-, and future studies should focus on the molecular mechanism of the increased uptake of NH4^＋ by NO3^-.     

氮肥施用对冬小麦籽粒产量和氮素表观损失的影响

Excessive nitrogen （N） fertilizer application to winter wheat is a common problem on the North China Plain. To determine the optimum fertilizer N rate for winter wheat production while minimizing N losses, field experiments were conducted for two growing seasons at eight sites, in Huimin County, Shandong Province, from 2001 to 2003. The optimum N rate for maximum grain yield was inversely related to the initial soil mineral N content （Nmin） in the top 90 cm of the soil profile before sowing. There was no yield response to the applied N at the three sites with high initial soil mineral N levels （average 212 kg N ha^-1）. The average optimum N rate was 96 kg N ha^-1 for the five sites with low initial soil Nmin （average 155 kg N ha^-1） before sowing. Residual nitrate N in the top 90 cm of the soil profile after harvest increased with increasing fertilizer N application rate. The apparent N losses during the wheat-growing season also increased with increasing N application rate. The average apparent N losses with the optimum N rates were less than 15 kg N ha^-1, whereas the farmers＇ conventional N application rate resulted in losses of more than 100 kg N ha^-1. Therefore, optimizing N use for winter wheat considerably reduced N losses to the environment without compromising crop yields.     

Nitrate leaching following autumn and winter application of animal manures to grassland

Abstract. Under a UK Government consultation procedure announced in 2001, it was proposed that measures agreed within already designated Nitrate Vulnerable Zones (NVZ 's) would be extended to include a considerably increased area of England, Wales and Scotland. Since existing NVZ 's in the UK have included relatively little grassland, it is important to examine how nitrate losses from grassland areas, especially from animal manures, one of the major potential sources of nitrate loss, can be minimized. Experiments were carried out on freely draining grassland soils at four sites (Devon, Hampshire, Shropshire and N Yorkshire) representative of a wide range of climatic and farming conditions across lowland England, over a four year period, 1990/91 to 1993/94. Slurry was applied to experimental plots over a range of times (including June and then monthly, from September to January) at a target rate of 200 kg N ha^–1. Nitrogen leaching over the four years ranged from 0 to >50% of applied slurry N, with the largest losses occurring following applications in the September to November period. The use of a nitrification inhibitor with slurry applied in November failed to provide consistent reduction in nitrate leaching.
A strategy to reduce the risk of N leaching from manures applied to freely draining grassland soils must take account of the characteristics of the manure, in particular its N content, the application rate and the amount of excess rainfall following application. The experimental results suggest that slurry applications to freely draining grassland, in September, October and November should generally be avoided, the rationale for this being dependent on the amount of excess rainfall subsequent to application. Farmyard manure represents a lower risk and does not justify the restrictions on application timing that appear to be necessary with slurry.     

硝酸镧、混合稀土对甘蔗叶片多酚氧化酶和过氧化物酶活性的影响

在3个甘蔗品种的分蘖初期,分别喷施硝酸镧和混合稀土溶液,结果这两种处理都能促使甘蔗叶片在不同生长期的多酚氧化酶活性极显著高于喷清水的对照,而且喷施混合稀土的又极显著高于硝酸镧处理。混合稀土还能使即将进入工艺成熟期的甘蔗叶片的过氧化物酶活性高于对照。     

供氮水平对菠菜产量、硝酸盐和草酸累积的影响

采用溶液培养方法研究了不同供氮水平对菠菜生物量、硝酸盐和不同形态草酸含量的影响。结果表明,供氮水平由4.mmol/L增加到8.mmol/L,菠菜产量显著增加,继续提高氮水平对产量没有显著影响。叶片中的维生素C(Vc)含量随着供氮浓度从4.mmol/L提高到8.mmol/L而显著增加,再增加氮水平,叶片中的Vc含量明显下降;而菠菜叶柄Vc的含量则随供氮水平的提高显著下降。叶片硝酸盐含量随着氮浓度的提高而增加,当供氮水平由4mmol/L增加到8.mmol/L时,叶柄硝酸盐含量显著下降,而氮水平由8.mmol/L提高到20.mmol/L时,叶柄硝酸盐含量则随之升高。供氮浓度从4.mmol/L增加到8.mmol/L,叶片可溶态草酸含量略有下降,再提高供氮水平则明显上升,供氮水平低于12.mmol/L时,叶柄中的可溶态草酸和菠菜叶片和叶柄中的草酸总量则随着氮水平的提高而升高,高于12.mmol/L草酸含量反而降低。由此可见,菠菜在供氮浓度为8mmol/L(N2)时能够获得较高的产量和Vc含量,较低的硝酸盐和草酸含量,表明适宜的供氮水平下可获得高产优质的菠菜。     

不同品种菠菜叶柄和叶片的硝态氮含量及其与植株生长的关系

温室盆栽试验研究了我国北方不同菠菜品种叶柄和叶片的硝态氮含量及其与植株生长的关系。结果表明,30个菠菜品种地上部分的生长量和硝态氮含量存在显著差异。叶柄和叶片在反映品种间生长量和硝态氮含量变异方面的作用并不相同。叶片占植株地上部鲜重的比例高于叶柄,品种间叶片生长量的差异亦大于叶柄,叶片与植株生长量的正相关关系更为显著。但与生长量的情况不同,叶柄的硝态氮含量、累积总量均显著高于叶片,是菠菜累积硝态氮的主要器官。叶柄硝态氮含量的品种间差异远大于叶片,与植株地上部硝态氮含量的正相关性更为显著。菠菜不同品种之间,叶柄硝态氮含量与地上部鲜重、干重及水分均表现出显著的正相关关系,而叶片硝态氮含量与植株生物量及其各组分之间却无这种关系。     

Risk assessment of nitrate pollution in groundwater based on the environmental nitrogen-assimilation capacity of agricultural lands in Hokkaido

(pp. 17–24)
A trial calculation was performed of the environmental nitrogen-assimilation capacity and the amount of nitrogen input based on various statistical data, which were compiled from each city, town and village in Hokkaido prefecture. The relationship between the excess quantity of nitrogen, after nitrogen input, and the environmental nitrogen-assimilation capacity and the nitrate-nitrogen concentration of the groundwater was considered.
Environmental nitrogen-assimilation capacity = nitrogen output by the crops + acceptable level of residual nitrate in the soil profile.*
^*It is calculated by the amount of nitrate precipitation evapotranspiration ×10 mg L⁻¹.

• 1) 

  The average value of the environmental nitrogen-assimilation capacity in Hokkaido Prefecture was observed to be 183 kg ha⁻¹. The maximum and minimum values of the environmental nitrogen-assimilation capacity were 308 kg ha⁻¹ and 94 kg ha⁻¹, respectively. When the average value of the environmental nitrogen-assimilation capacity with respect to main agricultural land use was compared across municipalities, it was largely in the following order · grassland (218 kg ha⁻¹), upland (169 kg ha⁻¹), and paddy land (157 kg ha⁻¹).

       

Critical drainage and nitrate leaching losses from manures applied to freely draining soils in Great Britain

Abstract. Pig slurry was applied by open-slot injection to experimental plots on a sandy loam site at ADAS Gleadthorpe, Nottinghamshire. Volume and distribution of over-winter drainage were adjusted through the use of rainfall exclusion covers or irrigation. The resultant slurry N leaching over the range of drainage values tested (up to 300 mm) could be satisfactorily described by curve-fitting, using a quadratic or exponential function. Initial simulations of slurry N leaching using the manure nitrogen decision support system manner (v. 3.0) compared poorly with the experimental data, predicting both earlier and greater amounts of nitrate leaching. However, the lack of fit could be explained by consideration of the likely ammonia emissions following slurry injection, the actual volumetric soil moisture capacity at the experimental site and the likely time delay for the nitrification of slurry N following application. Good agreement between modelled and observed data was achieved when these factors were taken into account. The manner model was used to simulate nitrate leaching beyond the range of drainage treatments tested in the experiments and the anticipated sigmoidal relationship between nitrate leaching and drainage was observed. The model was then used to study the effects of manure application timing and the likely impact on nitrate leaching, across the range of rainfall conditions found in Great Britain. Simulations for a range of manure types were undertaken, with manures applied at rates up to the limit of permitted N loading on freely draining sandy loams. Rainfall inputs for these simulations were based on long-term average climatic data. Results are presented for two contrasting manure types, cattle slurry and poultry manure, both of which are subject to controls in Nitrate Vulnerable Zones (NVZs) in Great Britain.     

日光温室番茄的氮素追施与反馈调控

在施用足量有机肥的基础上,以山东寿光当地的常规氮肥追施措施为对照,通过结合硝酸盐速测技术对日光温室秋冬茬番茄不同生育时期的氮素供应进行实时动态调控,以确定合理的追肥数量。结果表明,同当地农民的常规处理相比,采用实时调控处理的氮素投入总量减少了N170kg.hm2,但对番茄的生长(株高、茎粗、果实发育速度等)、产量和品质等指标没有任何影响。根据追肥前根层土壤氮素的供应水平(0—30cm土壤NO3--N含量+氮素追施数量)的监测,可初步确定日光温室秋冬茬番茄在第一穗果膨大期、第二穗果膨大期和第四穗果膨大期时的氮素供应目标值最多为N296kg.hm2、216kg.hm2和191kg.hm2。与此同时,将每个时期所确定的目标值应用在同期生长的其它3个日光温室的番茄氮素追施调控,结果发现与相应的农民常规处理相比,实时调控处理对作物的生长同样没有影响,这表明在日光温室秋冬茬番茄生产过程中,结合植株和土壤硝酸盐速测技术,通过施肥调控满足作物在不同生长时期的氮素供应水平是可行的。