中药"乳源康"药理作用研究

为了对“乳源康”的毒理药理学进行研究,进行4种试验——急性毒性试验、抗菌试验、局部刺激试验、抗炎试验。结果表明,①“乳源康”的小鼠口服LD50为36．80g／kg体重,腹腔注射LD50为13．85g／kg体重;②“乳源康”对乳房炎致病菌金黄色葡萄球菌、链球菌等有较强的抑制作用;③无明显刺激作用;④“乳源康”注射液与氢化可的松的消炎效果相同。“乳源康”治疗奶牛乳房炎具有高效、安全、低毒、使用方便、无化学药物和抗生素残留的优点,临床应用对治疗奶牛乳房炎、解决乳制品中的抗生素类药物残留以及病原微生物的耐药性问题具有重要意义。     

查干湖鲢源豚鼠气单胞菌分离鉴定及生物学特性研究

为了探讨2020年初吉林省查干湖分支甩湾子出现鲢大面积死亡现象的原因,从发病鲢中分离纯化出1株具有生长优势的菌株,并对分离菌株进行细菌形态观察和生理生化试验,用16SrDNA和管家基因的序列分析进行分子水平鉴定及系统进化树分析,之后对分离株进行携带毒力基因情况、生长曲线测定、人工感染试验及药敏试验.结果显示,分离菌株为...     

基于ISTA测试研究电商包装对莲雾货架品质的影响

莲雾果实皮薄肉脆,采后流通中极易产生机械损伤,严重影响果实货架品质。为了维持莲雾在物流运输中的货架品质,减少机械损伤,该研究基于ISTA 6-AMAZON.COM-SIOC TYPE A(ISTA 6A)测试标准模拟4种常用电商包装方式下（P1：瓦楞纸箱+单层网套、P2：瓦楞纸箱+双层网套、P3：瓦楞纸箱+发泡聚乙烯（expanded polyethylene,EPE）内衬和P4：泡沫箱+双层网套）莲雾果实的运输情况,并分析模拟测试后莲雾货架期（15℃,8 d）品质变化规律。结果表明,当外包装相同,缓冲形式不同时,双层网套（P2）组莲雾果实呼吸强度变化最为平缓,果皮亮度值为最低值32.86,果肉褐变指数（browning index,BI）与单层网套和EPE内衬相比上升了62.35%,其上升速率最慢,丙二醛含量（malondialdehyde,MDA）、过氧化物酶（peroxidase,POD）活性和多酚氧化酶（polyphenol oxidase,PPO）活性始终处于较低水平,货架品质显著好于单层网套（P1）和EPE内衬（P3）(P<0.05),原因在于单层网套的缓冲保护作用较...     

陕北黄土区不同植物根系抗拉力学特性研究

[目的]单根抗拉力学特性是植物发挥固土护坡作用的重要因素,乡土植物分布广泛对区域固土护坡起主要作用,研究比较不同植物单根抗拉力学特性,对坡陡沟深、滑塌频发的黄土区护坡植物选择具有重要意义。[方法]以陕北黄土区的截叶铁扫帚、猪毛蒿、大针茅、铁杆蒿、茭蒿和细叶薹草6种常见乡土植物为研究对象,通过室内单根拉伸试验,比较分析了以上6种植物不同根径单根抗拉力、单根抗拉强度和单根延伸率等力学特性。[结果](1)在根径范围内,6种植物单根抗拉力与根径呈幂函数形(y=ax^b,b>0)正相关关系,植物单根抗拉强度与根径呈幂函数形(y=ax^b,b<0)负相关关系;6种植物单根延伸率随着根径的增加呈现出增大趋势,但单根延伸率与根径之间未呈现出显著的相关性。(2)在试验根径0.05～0.60 mm范围内,植物种类对单根抗拉力、单根抗拉强度和单根延伸率的影响都达到显著水平,6种植物平均单根抗拉力关系为：细叶薹草(10.20 N)>大针茅(7.39 N)>铁杆蒿(6.87 N)>茭蒿(6.44 N)>猪毛蒿(6.34 N)>截叶...     

A comparison of the antinociceptive effects of xylazine, detomidine and romifidine on experimental pain in horses

Objective To study the analgesic potency of the α₂‐agonist romifidine in the horse using both an electrical current and a mechanical pressure model for nociceptive threshold testing. In addition, a comparison was made with doses of detomidine and xylazine that produce equivalent degrees of sedation. Study design Randomized, placebo‐controlled, blinded cross‐over study. Animals Six adult Swiss warmblood horses, one mare and five geldings, weighing from 530 to 650 kg and aged 6–15 years. Methods Nociceptive thresholds were measured using an electrical stimulus applied to the coronary band and using a pneumatically operated pin pressing on the cannon bone. Measurements were made immediately before and every 15 minutes for 2 hours after IV injection of the test substances. Lifting of the foot indicated the test end point. Results The three α₂‐agonists caused a temporary increase in nociceptive thresholds with a maximal effect within 15 minutes and a return to baseline levels within 1 hour. Using electrical current testing nociceptive thresholds were significantly different from placebo (mean ± SD) for detomidine at 15 minutes (from control 5.8 ± 0.9 to 23.3 ± 3.9 mA, p = 0.0066) and 30 minutes (from control 6.6 ± 1.1 to 18.8 ± 3.3 mA, p = 0.0091). The difference was significant for romifidine at 15 minutes only (from control 5.8 ± 0.9 to 18.7 ± 3.8 mA, p = 0.0066). With mechanical pressure testing nociceptive thresholds were significantly different from control for detomidine at 15 minutes (from 3.2 ± 0.2 to 6.2 ± 0.5 N, p = 0.00076) and 30 minutes (from 3.2 ± 0.7 to 5.7 ± 0.8 N, p = 0.0167). The difference was significant for xylazine at 15 minutes (from control 3.2 ± 0.2 to 5.6 ± 0.7 N, p = 0.0079). At 15 minutes the order of magnitude of the measured antinociceptive effect was significantly different between the two pain tests for both romifidine and detomidine, but not for xylazine. For romifidine, the increase of mean thresholds compared to placebo was 4.0 ± 1.3 times placebo levels with the electrical current test compared to 1.3 ± 0.3 times for the mechanical pressure test (p = 0.037). For detomidine, the increase of mean thresholds compared to placebo was 5.4 ± 1.7 times control levels with the electrical current test compared to 2.0 ± 0.2 times for the mechanical pressure test (p = 0.040). This represents a 2.7 (romifidine) and 3.4 times (detomidine) greater increase in thresholds using electrical current testing compared to the use of mechanical pressure testing. Conclusion and clinical relevance This study demonstrates the analgesic potential of α₂‐agonists in the horse for somatic pain and that they can have quantitatively different antinociceptive effects according to the antinociceptive test used.     

中华鳖致病性嗜水气单胞菌分离鉴定与药敏试验

为了探求中华鳖致病性菌株的药物体外抗菌效果,在江西进贤某中华鳖养殖场对病死中华鳖进行细菌分离,并对细菌进行形态特征、培养特性、生化鉴定、毒力因子检测试验、小白鼠毒力试验和人工感染等试验;采用K-B法药敏试验和96孔微型板二倍稀释法测定9种抗菌药物对该菌的最低抑菌浓度(MIC)来分析抗菌效果.结果表明:中华鳖为致病性嗜水...     

口蹄疫疫苗免疫效果跟踪试验报告

本试验用中农威特生物科技股份有限公司生产的猪O型口蹄疫灭活苗免疫两个不同的猪场,其中一个猪场由于母源抗体水平较高,免疫效果不理想,另一个猪场在免疫后36d、51d跟踪检测抗体,合格率为90%;用中农威特生物科技股份有限公司生产的O型-亚Ⅰ型口蹄疫二价灭活苗免疫两个不同的奶牛场,O型抗体水平明显高于亚Ⅰ型抗体水平。     

HPLC-PDA法测定17种兽药中非法添加多西环素

建立了17种兽药中非法添加多西环素的HPLC-PDA法。采用十八烷基键合硅胶为填充剂,以醋酸盐缓冲液-乙腈(80:20)为流动相,二极管阵列检测器进行全波长(200~400 inia)扫描,检测波长为280 nm,并通过液相色谱保留时间、紫外光谱信息和峰纯度检查,对非法添加物质进行定性鉴别和定量测定。结果表明,该色谱条件下,多西环素与其他物质峰分离良好。多西环素在5~500μg/mL浓度范围内线性良好,回收率在86.5%~118.1%之间,RSD为0.2%~4.5%,检测限为0.5 mg/g。本方法准确、可靠、重现性好,可用于兽药制剂中多西环素的定性和定量检测。     

钢-竹组合楼板受弯试验及变形分析

为了探讨界面滑移效应对钢-竹组合楼板变形的影响,进行6块组合楼板受弯性能试验.试件共分为2组,一组为冷弯薄壁型钢与竹胶板界面仅周结构胶复合,另一组则在胶粘基础上用自攻螺钉强化,对组合楼板破坏形态、承载能力、跨中变形、界面相对滑移等进行研究.分析表明：第一组试件考虑滑移效应的跨中变形计算值与实测值吻合较好,因考虑了跨中附加挠度的影响,理论计算结果与换算截面法相比,其精度大约提高5％,更符合实际情况;第二组由于自攻螺钉的强化作用,整体性能提高很多,材料进入塑形阶段,所建理论模型不符合试验情况,有待进一步研究.