小型猪特异性麻醉颉颃剂对大鼠脑NO/cGMP信号转导系统的动态影响

为探讨NO/cGMP信号转导系统对小型猪特异性麻醉颉颃剂催醒分子机理的调控,144只Wistar大鼠随机分为对照组和试验组,对照组和试验组再分为早期催醒组、中期催醒组和晚期催醒组。用分光光度法测定脑NOS活性和NO产量,ELISA测定脑cGMP含量。结果显示,小型猪特异性麻醉颉颃剂能明显地激活大鼠相关脑区的NOS活性和NO及cGMP产量,并且大鼠不同脑区NOS活性、NO产量和cGMP含量的变化趋势不仅与大鼠行为学变化相平行,还与不同时期表现出的催醒效果基本吻合。这表明小型猪特异性麻醉颉颃剂的催醒可能与激活特定脑区的NO/cGMP信号转导系统相关。     

滇南小耳猪与巴马小型猪SLA-DQA基因多态分析

对滇南小耳猪和巴马小型猪的SLA-DQA基因的部分内含子1、完整的外显子2和部分内含子2共341bp片段进行了PCR-RFLP酶切分型,结果表明：2品种经EcoR Ⅰ酶切后BB基因型频率（0.468 8）高于AB型（0.375 0）,AA型最低（0.156 3）,B为优势基因（0.656 3）,A为劣势基因（0.343 7）.经AluⅠ酶切后,滇南小耳猪MM基因型频率（0.500 0）高于MN型（0.321 4）和NN型（0.178 6）;而巴马猪则以MN基因型频率（0.485 3）高于MM型（0.338 2）和NN型（0.176 5）;2个品种中M等位基因（0.604 2）频率高于N等位基因（0.395 8）.经分析表明,2猪种在两酶切位点上各分型已达Hardy-Weinberg平衡.巴马小型猪和滇南小耳猪中分别存在9种和7种PCR-RFLP组合基因型,其中BBMN为优势组合基因型,AAMN为劣势组合基因型;AAMM组合基因型在2品种间差异显著（P＜0.05）.遗传多态参数分析表明：SLA-DQA基因外显子2的两酶切位点在2猪种间均表现为中度多态,AluⅠ的基因多样性略高于EcoRⅠ,巴马小型猪杂合性略高于滇南小耳猪,2品种间遗传距离为0.000 4.     

磷酸二酯酶在猪卵巢的表达与相关中药对其活性的影响

为了测定猪卵巢组织磷酸二酯酶（PDE）同工酶基因表达类型与活性规律，研究相关中药的作用机理，为繁殖障碍性疾病治疗积累资料。作者提取中国实验用小型猪卵巢组织总RNA，应用反转录聚合酶链反应（RT-PCR）测定PDE同工酶在其中的表达。选取催情散等复方及单味药，运用高效液相色谱法（HPLC）根据作用前后cAMP/cGMP含量的变化，分析对PDE活性的影响。结果检测的18个PDE亚型中除PDE4C外，PDE 1A、1B、1C、2A、3A、3B、4A、4B、4D、5A、7A、7B、8A、8B、9A、10A、11A mRNA在卵巢组织中均有表达。卵巢组织PDE活性较强，其中cGMP-PDE活性大于cAMP-PDE活性。催情散对卵巢组织cGMP-PDE活性均有较强的抑制作用，其中单味药淫阳藿作用最强。研究结果表明卵巢组织含有丰富的PDE同工酶，在通过环核苷酸水平卵巢机能调节中发挥着重要作用，中药催情散的作用与显著抑制cGMP-PDE活性有关。     

RADIOGRAPHIC ASPECTS OF Pneumocystis carinii PNEUMONIA IN THE MINIATURE DACHSHUND

The thoracic radiographic changes of Pneumocystis carinii in 7 miniature Dachshunds were reviewed. The dogs were 7–12 months old and presented with polypnea, exercise intolerance and clinical signs suggestive of immune-incompetence. P. carinii pneumonia was diagnosed in all the dogs using transtracheal aspirate cytology and confirmed at postmortem in 3 dogs that died. Radiographically, diffuse pulmonary changes were present and varied from a mild interstitial and bronchial pattern to an alveolar pattern. Radiographic evidence of cor pulmonale was present in 1 dog. The most severe radiographic changes were seen in 2 of the dogs that died.     

小场地足球对抗赛在足球训练中的应用

阐述了小场地足球对抗赛的特点和作用,对小场地足球对抗赛在足球训练(技术训练和战术训练)中的应用进行了研究,并对训练过程中应注意的问题进行分析。说明小场地足球对抗赛作为一种训练手段,应该被合理地应用到教学训练中去,因为这既是提高技战术水平和发展足球意识的需要,也是基本和重要的教学训练内容。     

Construction of a novel densitometer that utilizes a near-infrared laser system with Douglas fir (Pseudotsuga menziesii)

Abstract

A novel densitometer consisting of a continuous wave near-infrared (NIR) laser source and an avalanche photodiode module as the detector has been designed, which can rapidly and non-destructively measure the density of wood. The wood density of a small area (3.14 mm²) at the radial-transverse face was continuously estimated using the intensity of the transmitted light with the aid of the modified Lambert-Beer law. By conducting a validity evaluation with statistical coefficients, it was shown that the constructed system could obtain the sharp density profile compatible with X-ray densitometer [Root mean squared error of calibration (RMSEC) = 0.045 g cm^?3, Root mean squared error of validation (RMSEV) = 0.046 g cm^?3). It was concluded that the constructed NIR device has high performance from the viewpoint of operability, measuring time and safety.