貂出血性肺炎的实验室诊断

从病死貂的肝、心血中分离到一株革兰氏阴性杆菌,生化试验鉴定为铜绿假单胞菌。动物试验证明,该菌具有致病性。采用试管液体二倍稀释法测定了14种药物对该菌的药敏试验,从中筛选出有效药物,为临床防治该病提供了理论指导。     

HACCP在高校实验室安全管理中的应用及实践

为加强对高校实验室安全管理,对HACCP系统在实验室中的应用进行研究和探讨.以浙江医药高等专科学校为例.通过对实验室特点的分析,把HACCP体系引入到实验室安全的日常监管中来.通过危害分析,确定关键控制点,提出相应的控制措施.通过对实验室实施HACCP计划,显著提高了实验室的安全,为HACCP体系在高校实验室安全管理提供了可行的实践依据.     

豇豆轮纹病病原鉴定及其室内药剂筛选

从海南三亚豇豆设施棚内采集具典型轮纹病病斑的豇豆叶片,采用组织分离法分离到1株致病菌,根据病害症状特点、病原菌形态特征、培养性状、ITS序列分析,鉴定豇豆轮纹病的病原菌为多主棒孢霉[Corynespora cassiicola(Berk.&Curst.)Wei];利用生长速率法,对12种药剂进行室内筛选,结果表明:10%苯醚甲环唑(WP,可湿性粉剂)和50%异菌脲(WP)对病原菌具有强烈抑制作用,其EC50分别为2.709 46和9.093 27mg/L,而75%百菌清(WP)和50%多菌灵(WP)抑制效果较差,EC50分别为792.261和1 329.89 mg/L。     

Postgraduate training programs in veterinary clinical pathology in the United States and Canada (1998 to 2002)

Background: Residency and graduate programs in veterinary clinical pathology provide specialized training for board certification and are important pathways to careers in clinical pathology diagnostics, teaching, and research. Information about training opportunities is useful for assessing disciplinary needs, outcomes, and changes, garnering program support, and providing objective data for program evaluation by faculty, trainees, and prospective applicants. Objectives: The goals of this study were to 1) compile detailed information on the number and types of postgraduate training programs in veterinary clinical pathology in the United States and Canada, 2) describe the goals, activities, strengths, and weaknesses of the programs, 3) assess the desirability of program accreditation and program standards, 4) identify supplemental training opportunities, and 5) evaluate changes in programs, trainees, and faculty 4 years later. Methods: In July 1998, the American Society for Veterinary Clinical Pathology Education Committee sent a survey to representatives at the 31 schools and colleges of veterinary medicine in the United States and Canada and 31 diagnostic laboratories, private hospitals, and pharmaceutical companies. Survey data were compared with updated information obtained from training program coordinators in November 2002. Results: Survey response rate was 94% for universities, 39% for nonuniversity institutions, and 66% overall. In 1998, there were 20 clinical pathology training programs, including residencies (n=10) and graduate programs combined with residency training (n=10), with 36 total training positions. In 2002, there were 25 training programs (14 residencies, 11 combined), with 52 total positions. The median faculty: trainee ratio was 2.0 in both years. Of 67 faculty members involved in training in 1998, 57 (85.1%) were board‐certified in clinical pathology and 53 (79.1%) had DVM/PhD degrees. Net faculty numbers increased by 17 (25.4%) but the median per institution remained at 3.0. Primary program goals were 1) eligibility for and successful achievement of board certification in clinical pathology by the American College of Veterinary Pathologists, 2) proficiency in laboratory diagnostics, and 3) contemporary basic or applied research training. Many programs cited research opportunities, caseloads, and training in hematology and cytology as strengths. Program weaknesses included insufficient funding, too few faculty, and limited training in clinical chemistry and laboratory operations/quality assurance. Trainees completing programs within the past 5 years (n=70) were employed in academia (28.6%), diagnostic laboratories (32.9%), and industry (18.6%). For trainees completing programs between 1999 and 2002 (n=38), these percentages were 52.6%, 21.1%, and 7.9%, respectively. Most (62.5%) respondents supported program standards and accreditation, and 76% supported board review sessions for trainees. Conclusions: Opportunities for postgraduate training in veterinary clinical pathology increased between 1998 and 2002, with 5 new programs and 16 new training positions. These additions and the increased emphasis on diagnostic proficiency, efforts to strengthen training in clinical chemistry and quality assurance, and continuation of combined PhD‐residency programs will help address the perceived need for increased numbers of qualified clinical pathologists in academia, diagnostic laboratories, and industry.     

妃子笑荔枝上荔枝蒂蛀虫实验种群生命表的组建

通过田间笼罩、排除天敌等生物因子的作用,建立了荔枝蒂蛀虫实验种群生命表,分析结果表明,荔枝蒂蛀虫产卵期可持续11d,荔枝蒂蛀虫雌虫产卵量因幼虫取食寄主器官不同而不同,以妃子笑果实为食的可产卵160余粒,以嫩梢为食的仅产卵99粒,且卵孵化率较低,果期、梢期分别为80％和81％,果期幼虫死亡主要是生理性和钻蛀荔枝果皮过程死亡所致,死亡率在10％以下,蛹期不羽化率均较高,果期、梢期分别为15％和33％,在妃子笑果期和梢期,荔枝蒂蛀虫实验种群趋势指数分别为43.68和25.12。     

浅谈当前高校实验室的管理问题

本文概括了实验室管理的内容,以及当前高校实验室管理存在的普遍问题。提出健全实验室管理的规章制度和提高实验人员的综合素质是加强实验室管理的主要措施。     

Assessing redesigned effectiveness of the water quality monitoring program in the Indian River Lagoon,Florida

• 1. In 1996, the long‐term water quality monitoring network (WQMN) program in the Indian River Lagoon (IRL) of the St. Johns River Water Management District (SJRWMD) that began in 1988 was redesigned to eliminate statistically redundant sampling and satisfy modeling requirements.
• 2. Methods, procedures, and equipment were standardized across multi‐agency monitoring partners to produce reliable and comparable data. The redesigned strategy also included elements to determine previously unknown quantities such as differences in water quality parameters with depth and tidal cycle.
• 3. Reduction of sampling sites from 150 to 24 resulted in a significant improvement of standard deviation (t‐test; α = 0.05) for most water quality parameters analysed. The enhancement in data quality has demonstrated a more cost‐effective and efficient monitoring tool to measure the water quality of the seagrass environment in the IRL.
• 4. Results supported the view of a well‐mixed condition at the sampling stations on most days. Refocusing and streamlining the water quality monitoring program within the context of its mission would enable conversion of data into meaningful information regarding the interrelationship among water quality, photosynthetically active radiation (PAR), and seagrass PAR requirements.

Copyright © 2003 John Wiley & Sons, Ltd.     

兽医实验室仪器设备标识管理的现状及建议

实验室资质认定认可均对仪器设备标识管理提出了具体要求,可见标识管理在实验室质量管理中具有重要作用。通过分析兽医实验室仪器设备标识管理存在的问题,提出了规范化管理的仪器设备标识建议,以期为提高兽医实验室仪器设备标识管理水平提供参考。     

北方蚕区2014年春季桑蚕新品种实验室鉴定（河南点）报告

2014年春季,河南省蚕业科学研究院对鲁51×鲁52、优食一号这2对桑蚕品种进行了实验室联合鉴定。结合蚕期饲养、茧期调查、丝质鉴定,观察和总结这2对品种在河南试验点的饲育表现和性状特点。