槟榔间作条件下不同修剪方式对可可生长和产量的影响

以槟榔间作可可条件下的可可为研究对象,比较不同修剪方式对其生长、光合作用及产量的影响。结果表明:中度修剪提高可可株高、干周和主枝粗度,重度修剪和仅控冠幅降低可可干周和主枝粗度。中等修剪可可叶片Pn显著高于轻度修剪和仅控冠幅,可能由于修剪提高了新梢叶片叶肉细胞光合作用能力。中度修剪可可徒长枝重量分别比轻度修剪、重度修剪和仅控冠幅显著降低了69.27%、54.69%和57.03%,大大降低了养分消耗,提高产量的同时节省了修剪工作量。综上所述,在槟榔间作可可条件下,采用中度修剪有利于提高光合作用效率,形成健康平衡树形,降低徒长枝养分消耗,提高产量,为槟榔间作可可节本增效技术研究与优化提供依据。     

EPS364, a Novel Deep-Sea Bacterial Exopolysaccharide,Inhibits Liver Cancer Cell Growth and Adhesion

The prognosis of liver cancer was inferior among tumors. New medicine treatments are urgently needed. In this study, a novel exopolysaccharide EPS364 was purified from Vibrio alginolyticus 364, which was isolated from a deep-sea cold seep of the South China Sea. Further research showed that EPS364 consisted of mannose, glucosamine, gluconic acid, galactosamine and arabinose with a molar ratio of 5:9:3.4:0.5:0.8. The relative molecular weight of EPS364 was 14.8 kDa. Our results further revealed that EPS364 was a β-linked and phosphorylated polysaccharide. Notably, EPS364 exhibited a significant antitumor activity, with inducing apoptosis, dissipation of the mitochondrial membrane potential (MMP) and generation of reactive oxygen species (ROS) in Huh7.5 liver cancer cells. Proteomic and quantitative real-time PCR analyses indicated that EPS364 inhibited cancer cell growth and adhesion via targeting the FGF19-FGFR4 signaling pathway. These findings suggest that EPS364 is a promising antitumor agent for pharmacotherapy.     

Influencing in vitro clonal propagation of Chonemorpha fragrans(moon) Alston by culture media strength,plant growth regulators,carbon source and photo periodic incubation

Chonemorpha fragrans is an endangered medicinal woody climber,regarded among alternative plant sources of camptothecin.Camptothecin is a monoterpene indole anti-cancer alkaloid with annual trade value of over three billion U.S.dollars in the recent,and is used in the production of its analog drugs approved for the chemotherapy of cancer of varied types.Effects of plant growth regulators,culture media strength and photoperi-odic duration on the micropropagation ef ciency of C.fragrans from nodal segment explants were studied on Murashige and Skoog(MS)medium amended with Thidiazuron(TDZ),Benzylaminopurine(BAP)or Kinetin(Kin).Thidiazuron was more ef cient over BAP and Kin when half basal MS medium was used over full or quarter strength.Results of carbon source experiment showed sucrose as the most effective over glucose,fructose,and maltose in the clonal production.Studies on the photope-riodic incubation duration showed 12 h as the best light period and sub or supra-optimal resulted in the production of abnormal and albino micro shoots.Experimental results on the evaluation of physiological,biochemical parameters showed the role of pigment molecules and antioxidant systems in the production of albino micro shoots.     

辣椒新品种‘秦椒1号’

‘秦椒1号’辣椒是以自交系‘R9816-2-1-3’为母本,‘Z97-2-7-42’为父本杂交育成的一代杂种。果实长灯笼形,表面微皱,果长17.1 cm,果肩宽4.7 cm,果肉厚3.1 mm,单果质量73.5 g,商品性好,品质佳,平均产量45 062.6 kg·hm~(-2)。高抗病毒病、青枯病和疫病。     

水稻对稻飞虱抵御机制研究

为更好地利用水稻自身抵御能力遏制稻飞虱为害,综述了水稻抵御稻飞虱的物理、生化效应和机制的研究现状。水稻抵御稻飞虱的物理机制包括宏观的水稻叶脉间距、宽度,钩毛和纤细毛密度、长度,硅含量和微观的粗纤维、胼胝质含量及叶绿体膜稳定性;生化机制涉及水分、可溶性糖、叶绿素、游离氨基、酶物质活性及挥发物的含量。国内外虽广泛涉及水稻自身固有的物理结构及体内生理生化物质对稻飞虱的抵御效应研究,但其分子机理研究较为匮乏,尚需深入系统的研究。     

保护地黄瓜新品种唐杂6 号的选育

唐杂6 号是以雌性系S16 为母本，以自交系S26 为父本配制的强雌型黄瓜一代杂种。生长势强，商品瓜短棒状， 瓜长12～14 cm，横径4.0～4.3 cm，非特异性环境下雌花率95% 以上，瓜皮嫩绿有光泽，白刺，刺瘤稀小，平均单瓜质量 131.8 g 左右，高抗霜霉病，抗细菌性角斑病，耐白粉病；春保护地栽培平均产量可达8 000 kg·（667 m²） ^-1，秋冬保护地栽 培产量可达6 500 kg·（667 m²） ^-1；适合河北、北京、天津及东北地区春、秋保护地种植。     

不同氮肥水平下蚯蚓粪施用对水稻生长特性及产量的影响

以两优培九为材料,采用大田微区试验,研究不同氮肥水平(N1,9 g/m~2;N2,12 g/m~2;N3,15 g/m~2)下不同蚯蚓粪施用量(EC0,0;EC_1,16.88 kg/m~2;EC_2,33.76 kg/m~2)对水稻生长及产量形成的影响。结果表明,移栽40 d后EC_2和EC_1处理的茎蘖数均显著高于EC0(P0.05);幼穗分化期和乳熟期EC0处理的SPAD值均显著低于EC_1和EC_2处理(P0.05);齐穗期EC0处理的叶面积指数分别比EC_1和EC_2处理低32.7%和80.7%,地上部总干物质量分别比EC_1和EC_2处理低30.6%和59.8%;成熟期有效穗数、地上部干物质量和水稻产量均表现为EC_2EC_1EC0,EC_2和EC_1处理的有效穗数分别比EC0处理多41.5%和21.3%、地上部干物质量分别比EC0处理高47.7%和25.8%、产量分别比EC0处理高35.4%和34.3%。由此可见,施用蚯蚓粪便可促进水稻分蘖,提高水稻叶面积、SPAD值和地上部干物质量,进而提高水稻籽粒产量。     

Growth and yield of self-compatible and hybrid common buckwheat lines pollinated with and without flies

Common buckwheat is a self-incompatible, insect-pollinated allogamous plant. This study examined growth and yield of the common buckwheat self-compatible and hybrid lines pollinated with and without flies. Self-compatible ‘IH3’, hybrid ‘IP2/IH3’, and standard self-incompatible ‘Kitawasesoba’ were used in field and pot experiments. Self-compatibility of ‘IH3’ was shown to be of high purity. Approximately 10% segregation of pin plants from ‘IP2/IH3’ was observed. The harvest index of ‘Kitawasesoba’ pollinated without flies was considerably lower than that pollinated with flies in both field and pot experiments. The harvest index values of ‘IH3’ and ‘IP2/IH3’ were hardly affected by the presence or absence of flies. The morphological traits of ‘IH3’ were significantly lower than those of the other two genotypes. Large differences in these traits between ‘IP2/IH3’ and ‘Kitawasesoba’ were not observed in either the field or pot experiments. The seed yield of ‘Kitawasesoba’ pollinated without flies tended to be lower than that pollinated with flies. The seed yield of ‘IP2/IH3’ tended to be higher than that of ‘IH3’. The hybrid line ‘IP2/IH3’ showed a high fertilization rate, which was nearly as high as that of ‘IH3’. Rate of fertilization and percentage of ripe seeds were higher in ‘IH3’ and ‘IP2/IH3’, wherein they were hardly affected by the presence or absence of flies, than in ‘Kitawasesoba’. The ‘IP2/IH3’ hybrid line will be useful for understanding the stable high-yielding ability of self-compatibility common buckwheat.     

基于RGB颜色空间的早稻氮素营养监测研究

针对双季稻区水稻过量施肥带来环境污染和成本提高问题,设计不同品种氮肥梯度大田试验,应用数码相机获取早稻冠层数字图像,研究不同色彩参数及早稻氮素营养指标的时空变化特征,以期确立双季早稻氮素营养预测模型。结果表明:不同品种同一氮肥处理下图像色彩参数差异不大;拔节期数字图像参数对氮素营养指标敏感;模型构建结果显示,图像参数INT与水稻氮素营养指标构建的模型决定系数(R2)最大,模型预测效果最佳,R2分别为0.895 7和0.924 7;进一步采用多元回归分析和BP神经网络分析法进行预测,预测效果均较好。对预测结果进行检验,发现品种对于模型的构建影响不大,以BP神经网络分析法构建的叶片氮浓度(LNC)模型和以INT为敏感色彩参数构建的叶片氮积累量(LNA)回归模型效果最优,而多元回归分析方法则效果不佳。早稻冠层RGB颜色空间敏感参数与氮素营养指标间相关性较好,可以实现氮素营养的无损监测诊断。     

不同类型水稻品种产量和氮素吸收利用对大气CO2浓度升高响应的差异

目的 探明不同类型水稻品种产量和氮素吸收利用对FACE(大气CO₂浓度增高)响应的差异。方法 以常规粳稻、杂交籼稻、常规籼稻共6个品种为供试材料,研究FACE对不同类型水稻产量、氮素吸收利用的影响。结果 1）FACE处理极显著提高了水稻产量,平均增加24.17%, 常规籼稻增幅最大,FACE和对照均以杂交籼稻最高;2）FACE处理显著增加了单位面积穗数,常规粳稻增幅最大,并显著增加了杂交籼稻和常规籼稻每穗粒数;3）FACE处理显著提高了成熟期吸氮量和氮素籽粒生产效率,成熟期吸氮量平均增加21.23%,杂交籼稻增幅最大, FACE和对照均以常规籼稻最高;氮素籽粒生产效率平均增加7.33%,杂交籼稻增幅最大,FACE和对照均以杂交籼稻最高。成熟期吸氮量对产量促进作用略大于成熟期氮素籽粒生产效率;4）FACE处理降低了植株含氮率,成熟期平均下降0.105个百分点,常规粳稻降幅最大。FACE处理极显著提高植株干物质量,成熟期平均增加23.95%,常规籼稻增幅最大;FACE处理显著提高常规籼稻和杂交籼稻成熟期单穗吸氮量,分别增加10.79%、13.93%,但常规粳稻下降了9.60%;FACE处理显著提高了成熟期群体吸氮强度,平均增加22.29%,杂交籼稻增幅最大。FACE处理对水稻全生育期天数无显著影响;FACE处理显著提高茎鞘、叶片、穗各器官吸氮量,叶片增幅最大,平均增加51.86%,杂交籼稻增幅最大;FACE处理显著提高了不同生育阶段吸氮量,抽穗-成熟阶段增幅最大,平均增加108.90%,杂交籼稻增幅最大;5）植株干物质量、单穗吸氮量、吸氮强度、穗吸氮量、抽穗-成熟阶段吸氮量对成熟期总吸氮量的促进作用分别大于植株含氮率、单位面积穗数、生育天数、茎鞘叶吸氮量、移栽-分蘖和分蘖-抽穗阶段吸氮量;6）FACE处理显著提高了氮肥偏生产力,降低了每百千克籽粒需氮量,前者平均增加24.16%,常规籼稻增加最多;后者平均降低4.7%,常规籼稻降幅最大。结论 FACE处理可显著提高水稻产量和氮素吸收利用效率,但品种间差异较大。