嗜热真菌M5的生物学特性

助堆剂中一种嗜热真菌Ｍ５的最适生长温度为４５－５０℃，ＰＨ７．５－８．０，经它分解代谢后的草料培养基及其浸出物，可以促进蘑菇菌丝的生长繁殖。Ｍ５具有堆制作用。     

黄空柄牛肝菌菌丝体纯培养条件下营养生理特性的初步研究

黄空柄牛肝菌是油松能够在干旱石质山区生长的关键菌根真菌之一。经组织分离与菌丝体的纯培养，其营养生理代谢特性的初步研究结果表明：培养基、Ｃ、Ｎ源、ＰＨ值对菌丝体的生长均为极显著，菌丝体在ＭＲＤ培养上基生长势最好，酒石酸氨是其生长的最佳Ｎ源，同时该地有机Ｎ源的利用效好的效果。     

死活相克原理与“菌根遗毒”假说──视解杉木连栽引起地力衰退之谜

根据多年的实践观察，应用“死活相克”原理，提出“菌根遗毒”的假说，剖析杉木连栽引起地力衰退问题的一些原因，这将对该问题认识的深入与解决有一定的促进作用。     

园林花卉真菌病害研究之五

 本文报道15种园林花卉植物上15种真菌病害,其中国内新记录5种,云南、四川、新疆、省区新记录10种,国内新记录种有描述附病原图,标本存云南农业大学植物病理研究室真菌标本室(MHYAU).     

黑老虎枝枯病病原鉴定及其生物学特性

Kadsura coccinea (Lem.) A. C. Smith has been wildly cultivated in Southeast of Guizhou province, bearing fruit as an excellent nutrition source being rich in vitamin C, vitamin E, and a variety of trace elements. In 2017-2018, we found twig blight on Kadsura coccinea with small black dot-like structure in the late stage on lesion, a very common disease causing significant economic losses. The fungal isolates were recovered from the symptomatic stem tissues and small black dot (pycnidium) which contained transparent, unicellular and spindle-shaped conidia of (17.4±1.2) μm×(6.5±0.7) μm. The purified culture with the grayish white to dark grayish-green as well as partial straight hyphae on PDA plate was consistent with Neofusicoccum parvum, supported by the aliment and phylogenetic analyses of ITS, Ef1 α and Tub2 gene sequences (GenBank no: MK563984、MK563986 and MK563987).The pathogenicity test on leaves and stems with needle-punching method matched the symptoms described above and re-isolated the fungus confirming the Koch’s postulate. The lethal temperature for mycelial growth was 54℃ for 10 min; The strain could grow normally in most carbon and nitrogen sources except ammonium carbonate. This is the first report of Neofusicoccum parvum causing twig blight on Kadsura coccinea in China.     

稗叶枯菌及其毒素的研究——Ⅱ.病菌寄主范围和对稗的致病性

病原菌孢子悬浮液经人工接种可侵染稗、高梁、玉米和大麦,但不侵染水稻、小麦和小米.病菌的培养滤液稀释液对稗、高粱、小米表现毒性,对水稻、小麦、玉米、大麦不表现毒性.用培养滤液配成的孢子悬浮液接种2叶期稗苗.10d 后最高死苗率为60.5%,病情指数为86.63.     

血耳生物学特性的研究

血耳是民间极感性趣的一种药用兼食用菌。本文从温度、湿度、pH、光线、水份、碳源、氮源、培养代料等因子对血耳菌丝生长原基分化、出耳的影响进行了生物学特性方面的研究,为开发利用血耳,进行人工栽培提供资料。     

白雷蘑菌丝生物学特性的初步研究

白雷蘑是山西省著名的野生食用菌．该菌营养丰富、味道鲜美。为了开发利用这一自然资源,我们对其菌丝体生物学特性进行了研究。实验表明．以腐殖土浸出液制成的葡萄糖酵母青琼脂(DYA)加富培养基是其母种的适宜培养基．原种适宜培养基配方是杂木屑40％．棉籽壳40％,麸皮15％,腐殖土5％。最适pH为6～7,最适温度是21～23℃．培养过程中,4～6℃的低温处理对促进菌丝生长有较好作用。     

Successful treatment of disseminated cutaneous phaeohyphomycosis in a dog

A 7-year-old castrated male Whippet developed deep ulcerative skin lesions whilst receiving immunosuppressive doses of prednisolone and cyclosporine for the treatment of immune-mediated haemolytic anaemia. The lesions were determined to be a phaeohyphomycosis, caused by Curvularia lunata. The dog was treated with a combination of systemic antifungals and weaning off immunosuppressants and made a complete recovery. To the authors' knowledge, this is the first case report of the successful treatment of disseminated cutaneous phaeohyphomycosis in a dog.     

芦笋茎枯病菌的鉴定及区域差异性分析

芦笋茎枯病是一种世界性分布的毁灭性病害,为准确鉴定其致病菌和探明不同区域菌株的分化程度,通过形态学观察和核糖体DNA内转录间区(rDNA ITS)序列比对进行形态学和分子生物学鉴定,比较五省份菌株的生物学特性和ITS序列上的差异,并进行系统发育分析。结果表明,芦笋茎枯病的致病菌为天门冬拟茎点霉Phomopsis asparagi。海南省菌株的菌丝生长较快,培养5天后的平均直径为8.5 cm;培养14天后,江西省菌株由白色变为淡绿色,其它各省菌株由白色变为灰白色,海南省菌株的菌落呈现同心轮纹状;福建省菌株产生的分生孢子器较多,平均60个/皿;五省菌株在1～238 bp和298～591 bp的ITS区段存在差异性碱基,其中河北省菌株的差异性碱基数最多;五省菌株大致聚为2个组群,河北省菌株单独聚为1个组群,其它省份菌株聚为1个组群,天门冬拟茎点霉P.asparagi与叶下珠生拟茎点霉P.phyllanthicola亲缘关系较近。