Age-related changes in hematology and plasma chemistry values of hybrid striped bass (Morone chrysops X Morone saxatilis)

Hybrid striped bass (Morone chrysops X Morone saxatilis ) are an important aquaculture species yet there are few diagnostic tools available to assess their health. Hematology and clinical chemistry analyses are not used extensively in fish medicine due to the lack of reference intervals for various fish species, and because factors such as age can affect blood values. There is little published information regarding age-related changes in blood values of juvenile fish. It is important to evaluate juvenile fish, as this is the time they are raised in aquaculture settings. Determining age-related changes in the blood values of fishes would further develop clinical pathology as a diagnostic tool, enhancing both fish medicine and the aquaculture industry. The results of standard hematology and clinical chemistry analysis were evaluated in juvenile hybrid striped bass at 4, 6, 9, 15, and 19 months of age. Values for PCV and RBC indices were significantly lower, and plasma protein concentration was significantly higher in younger fish. Total WBC and lymphocyte counts were significantly higher in fish at 6 and 9 months of age, while neutrophil and monocyte counts were higher at 6, 9, and 15 months. Eosinophil counts were significantly higher in 9-month-old fish. The majority of hematologic values fell within previously established reference intervals, indicating that only slight modification to the intervals is necessary for evaluating hematologic results of hybrid striped bass at different ages. The following analytes deviated sufficiently from adult reference intervals to warrant separate reference values: plasma protein concentration at 4 months, WBC and lymphocyte counts at 15 and 19 months, and thrombocyte-like-cells at 9 months of age. Values for most biochemical analytes were significantly different among age groups except for creatinine and potassium concentrations. Comparisons with reference intervals were not made for biochemical analytes, because established reference intervals were not available. Age-related changes in hematologic and biochemical values of striped bass were similar to those reported for rainbow trout and mammals.     

Blood chemistry values of juvenile red pacu (Piaractus brachypomus)

Abstract: Blood samples were collected from 29 juvenile red pacu ( Piaractus brachypomus ), ornamental freshwater fish, to establish baseline blood chemistry values. Mean (minimum-maximum) values, obtained by automated bichromatic analysis and ion selective electrode analysis, were as follows: sodium, 150.4 (146–159) mmol/L; potassium, 3.93 (2.7–5.0) mmol/L; chloride, 138.7 (128–150) mmol/L; total CO₂, 7.5 (6–10) mmol/L; albumin, 0.86 (0.5–1.0) g/dL; lactate dehydrogenase, 237.8 (65–692) IU/L; aspartate aminotransferase, 49.1 (0–125) IU/L; creatinine, 0.31 (0.2–0.4) mg/dL; calcium, 10.80 (9.5–12.5) mg/dL; anion gap, 6.89 (1.2–12.5) mmol/L; and phosphorus, 7.29 (4.1–8.9) mg/dL.     

Effect of pyrethroids on carbohydrate metabolic pathways in common carp,Cyprinus carpio

The activity levels of succinate dehydrogenase (SDH) and glucose‐6‐phosphate dehydrogenase (G6PD) were assessed in various tissues of Cyprinus carpio var communis which had been exposed to lethal concentrations of group‐II pyrethroids (deltamethrin, cypermethrin, fenvalerate and fluvalinate) for a period of 72 h. The results indicated a steady decrease in SDH activity with a concomitant increase in G6PD activity. The decreased SDH activity indicated inhibition of SDH at mitochondrial level and the increased G6PD activity an enhancement of an alternative pathway of carbohydrate metabolism, viz the hexose monophosphate shunt (HMP) or pentose phosphate pathway as a biochemical adaptation to overcome the toxic stress. © 2001 Society of Chemical Industry     

百倍冲水库种草养鱼防淤试验初报

百倍冲水库自1976年修建以来,养鱼由于饲料长期缺乏,300亩养鱼水面常年产鲜鱼1000kg左右,库内每年淤积泥砂0.6～0.8万立方米。作者于1985～1987年在库内的消落区种植黑麦草,紫云英;荒山种象草、扁穗牛鞭草;堤坝上种岸杂一号狗牙根;幼林地种鸡脚草、拉丁鲁白三叶等牧草配套养鱼和防止水土流失进行试验研究。结果,库内年鲜鱼产量由种草前1000kg提高到7000～8000kg,泥砂淤积量每年减少0.516万立方米,经济生态效益显著。     

清塘净对鱼类等水生生物急性致毒的研究

清塘净对鱼类等水生生物毒性强，毒力降解速度较快，是好的清塘药物。对黄鳝等水生生物９６ｈ的ＬＣ５０及安全质量浓度分别为：黄鳝２．４１ｍｇ／Ｌ和０．２４１ｍｇ／Ｌ；鲤鱼苗０．３８ｍｇ／Ｌ和０．０３８ｍｇ／Ｌ；草鱼苗０．４１ｍｇ／Ｌ和０．０４１ｍｇ／Ｌ；大型蚤０．５２ｍｇ／Ｌ和０．０５２ｍｇ／Ｌ；湖螺２．３３ｍｇ／Ｌ和０．２３３ｍｇ／Ｌ；泥鳅３．０４ｍｇ／Ｌ和０．３０４ｍｇ／Ｌ。     

鱼类主要内分泌激素和蛋白质代谢的关系

本文就鱼类主要的内分泌激素(T3、T4、GH、IGF-Ⅰ)对蛋白质代谢的调控以及饲料蛋白质质量和数量对鱼类内分泌激素分泌的调控进行概述。     

鱼类对饲料中糖的利用研究进展

糖类是鱼类饲料中廉价的能量源,然而,与陆生动物相比,鱼类对饲料中糖的利用能力较低。本文综述了这方面的最新研究进展,包括鱼类对饲料糖的利用能力与鱼饲料中糖的适宜含量、影响因素及鱼类的糖代谢机制。     

喷雾干燥鱼浆蛋白粉营养成分分析

本试验对鱼粉加工废水鱼浆蛋白粉(经浓缩、水解、喷雾干燥得到的副产品)进行营养成分分析,其中粗蛋白质含量达64.08%,钙2.67%、磷1.12%,钙磷比例合理;各氨基酸含量分别为:赖氨酸3.29%,蛋氨酸0.94%,谷氨酸5.17%,牛磺酸2.50%。     

用改进的酚-氯仿法提取鱼类基因组DNA效果的分析

提取鱼类基因组DNA的研究报道并不多见,效果也各有差异。本文以泰山螭霖鱼、东平湖鲤鱼、东平湖鲫鱼为材料,采用改进的酚-氯仿法提取基因组DNA,并进行了紫外分光光度、琼脂糖凝胶电泳、微卫星PCR扩增等方法的鉴定。结果表明,本方法提取的基因组DNA浓度在0.9μg/μL~3.25μg/μL之间,A260/A280比值在1.79~1.87之间,电泳条带整齐明亮,适合微卫星PCR扩增,且整个操作过程使用试剂较少,简单易行。     

我国综合养鱼生态模式及其发展思路

我国主要的综合养鱼生态模式有渔农综合经营型,渔牧综合经营型,渔、农、牧综合经营,农庄式立体种养生态系统等。然而劳动力素质限制了其综合利用水平的提高,水质污染影响渔业可持续发展,综合养鱼生态系统基础研究滞后及渔业产业化经营程度不高均是亟待解决的问题。并指出了促进综合养鱼生态模式的发展思路。