泰州地区乡村旅游营销策略分析

针对泰州地区乡村旅游的现状,运用4P理论分析了目前制约泰州地区乡村旅游发展的若干问题,如乡村旅游产品单一、价格定位不当、促销手段简单、销售渠道狭隘等,并且在此基础上有针对性地提出泰州地区乡村旅游发展的营销策略。     

Amplification,Sequence Analysis and Tissue Expression Detection of FUT2 Gene Coding Regions of Dongchuan Pigs

Porcine alpha (1,2) fucosyltransferase (FUT2) gene was importance in glycosphingolipid biosynthesis-globo series, potentially played a regulatory role during Escherichia coli (E. coli) F18 infection process in weaned piglets. In order to explore sequence structure of porcine FUT2 gene and its biological function, this test amplified FUT2 gene CDS sequence of Dongchuan pigs by PCR, forecasted and analyzed the protein sequences and functional regions of FUT2 gene, its expression level was detected in 11 tissues of 8 Dongchuan weaned piglets in 35 days old at the meantime. The results showed that the CDS sequence of FUT2 gene was 1 023 bp, which encoded 340 amino acids. FUT2 protein was fat-soluble hydrophilic protein, which the structure was not stable, including a transmembrane helix structure, but without signal peptide that suggested the FUT2 protein was a membrane protein;FUT2 protein included 2 N-glycosylation sites (No. 185 and No. 305 amino acids), without O-glycosylation sites, there were 14 potential phosphorylation sites, included 6 Ser, 2 Thr and 6 Tyr, analyzing the functional regions found that the FUT2 protein had a superfamily of conserved domains:FUT1-FUT2-like (58-319 amino acids). The phylogenetic tree result showed that the relative relationship between swine and cattle was relatively close, but was distant from chimpanzee, human, mouse and rat. FUT2 gene was expressed in all 11 tissues of Dongchuan weaned piglets, there were higher expression in digestive tract and immune tissues. The present results suggested that FUT2 gene might play a role to resistance to E. coli F18 in weaned piglets, and might indirectly against E. coli F18 through the synthesis of fucosyltransferase.     

农田管理措施对滨海盐渍化土壤碳平衡的影响

为研究不同农田管理措施对滨海盐渍土壤碳平衡的影响,通过玉米-小麦轮作试验,研究农田土壤碳收支情况。试验共设6个处理:(1)常规对照(CK),(2)有机肥常量(OF),(3)氮肥增施(NF),(4)秸秆还田(S),(5)有机肥加秸秆(OF+S),(6)免耕(NT)。结果表明,秸秆还田和施用有机肥提高了土壤呼吸的强度,而NT处理的CO_2平均释放量最低,不同处理下土壤呼吸表现为OF+S处理S处理OM处理NF处理CKNT处理。各处理土壤有机碳量随着作物种植年份的增加而逐渐升高,其中OF与NT处理增加最多,而NF处理并没有显著提高土壤的有机碳量。在两季作物收获后,各处理的碳输入均高于碳输出,表现为碳净输入,呈现较强的碳汇特征。S处理和OF处理的碳净输入均显著高于CK,可有效减缓土壤CO_2排放,增加其有机碳的输入。     

探析IPv6技术

阐述了IPv4现存在的一些问题和IPv6的一些优点,在此基础上对IPv6、DNS进行简单的介绍。     

PRV、PCV-2、PRRSV与HPs混合感染的核酸检测

为确定青海省某规模化发病猪场的发病原因,在临床诊断的基础上对采集的临床组织病料进行了13种常见病原体的核酸检测,并对检测结果进行了序列鉴定.结果表明,该发病猪场存在猪伪狂犬病毒、猪圆环病毒2型、猪繁殖与呼吸综合征病毒与副猪嗜血杆菌的混合感染.研究结果提示,利用分子生物学技术对发病猪场的病原进行快速鉴定,是病源快速诊断和防控的有效途径.     

甘蔗蜡提取物多廿烷醇的降血脂机制研究

采用RT-PCR、Western Blot、L-[35S]放射性标记物掺入等方法,观察甘蔗蜡提取物多廿烷醇对Hep G2细胞羟甲基戊二酸辅酶A (HMG-CoA)还原酶mRNA转录及蛋白质表达量的影响,及其对HMG-CoA 还原酶合成和降解的作用.结果表明,多廿烷醇降低HMG-CoA还原酶mRNA的转录和蛋白质的表达量,且作用效果随多廿烷醇浓度的升高而增强.此外,多廿烷醇抑制HMG-CoA还原酶的合成并加速其降解,多廿烷醇处理后HMG-CoA还原酶的半衰期明显缩短.     

砂土抗剪强度数值模拟的关键技术研究

利用二维颗粒流软件PFC 2D对砂土的抗剪强度进行研究。通过建立数值试样模型、设立相对合理参数、引用＂橡皮膜＂程序等方法对砂土的三轴剪切试验进行数值模拟,模拟的结果较好地反映了砂土的力学性质。     

Pb2+对甘肃纹党种子萌发和幼苗生理特性的影响

以甘肃道地中药材纹党(Codonopsis pilosula)为对象，通过室内培养试验，研究不同浓度10^-6、10^-5、10^-4、10^-3 mol/L Pb²⁺处理对纹党种子萌发、幼苗生长以及生理特性的影响，为探究Pb²⁺污染对纹党品质的影响提供依据。结果表明，Pb²⁺对纹党种子吸胀过程中相对吸水量的增加有抑制作用，随着Pb²⁺浓度的提高，党参种子的萌发率、萌发指数、幼苗生长及根尖微核和有丝分裂率变化，表现出先升高后降低的趋势；低浓度的Pb²⁺处理能够促进叶绿素的形成，高浓度则抑制叶绿素形成；可溶性糖、游离脯氨酸和丙二醛含量，随Pb²⁺浓度的升高而增加；Pb²⁺对SOD、POD和CAT活性有较强的抑制作用。可见，生物测定结果大多达到显著差异水平。     

Response of seedlings growth of Pinus sylvestriformis to atmospheric CO_2 enrichment in Changbai Mountain

IntroductionGIobaIchangehasbeenoneoftheimpoFtantis-suesandscientistspaycIoseattentiontoit.ThegreatimportanceofforestecosystemisreflectednotonlybytheirhUgebiomass,butaIsobytheirsignifi-cantroIeintheglobaIcarbonbaIance.Howtreesrespondtoclimaticchangesmightbeofgreatsig-nificance.ManystudiesindicatethattherisingatmosphericCo,leveIscanmakeasubstantialeffectonplantgroWthanddevelopment.SomeonethinkcommonlythattherisingCo,levelscanstimuIatepIantgroWthandbiomassproduction,sincephotosynthesisofC…