The biochemical control of the cell cycle by growth regulators in higher plants

The cell cycle is an important research field in cell biology and it is genetically and developmentally regulated in animals and plants. The aim of this study was to review knowledge about the biochemical regulation of the cell cycle by plant growth regulators through molecular checkpoints that regulate the transition from G0-G1-S-phase and G2-M in higher plants.Recent research has shown that zeatin treatment led to the up-regulation of CycD3 in Arabidopsis. Benzyladenine treatment can also shorten the duration of S-phase through recruitment of latent origins of DNA replication. Kinetin is involved in the phosphoregulation of the G2-M checkpoint; the major cyclin-dependent kinase (Cdk) at this checkpoint has recently shown to be dephosphorylated as a result of cytokinin treatment, an effect that can also be mimicked by the fission yeast Cdc25 phosphatase. Gibberellic acid (GA) treatment induces internode elongation in deepwater rice, this response is mediated by a GA-induced up-regulation of a cyclin-Cdk at the G2-M checkpoint. Recent evidence has also linked abscisic acid to a cyclin-dependent kinase inhibitor. A new D-type cyclin, recently discovered in Arabidopsis may have a key role in this process. A brief review on plant growth regulator-cell cycle interfacing during development and a cytokinin-induced continuum of cell cycle activation through the up-regulation of a plant D-type cyclin at the G1 checkpoint and the phosphoregulation of the Cdk at the G2/M checkpoint had been concluded. This review could be valuable to research on cell and developmental biology in plants.     

云南松纵坑切梢小蠹危害及生物学特性研究

纵坑切梢小蠹Ｔｏｍｉｃｕｓｐｉｎｉｐｅｒｄａ在云南分布很广，８０年代以来，在昆明、红河、楚雄３地、州３０个县的云南松人工林内猖獗危害，直接经济损失６０００余万元。该虫在云南１ａ一代，全年危害，无明显的越冬现象。每年５～１１月蛀梢，１１～翌年５月蛀干，树皮下各虫态种群高峰期出现在２～５月，此时是清理蠹害木的最佳时期。成虫干转梢及由梢转干在林间扬飞期１～１４ｄ，集中扬飞３～４ｄ，扬飞期对防治成虫有重要意义     

刨花板板坯表面增湿处理—缩短热压周期的有效途径

本文阐明，刨花板板坯表面增湿是缩短热压周期的有效途径及其原理，影响增湿效果的主要因素是热压板温度、增湿量、增湿层厚度比。     

森林生态系统碳循环研究方法概述

介绍了森林生态系统碳循环研究方法,对当前森林生态系统碳循环研究的几种主要方法模型进行了概述,主要有Miami模型和Thornthwaite模型、Chikugo模型和生物量转换因子连续法等。     

Energy and carbon dioxide (CO2) balance of logging residues as alternative energy resources: system analysis based on the method of a life cycle inventory (LCI) analysis

Using the method of a life cycle inventory (LCI) analysis, the energy balance and the carbon dioxide (CO₂) emission of logging residues from Japanese conventional forestry as alternative energy resources were analyzed over the entire life cycle of the residues. The fuel consumption for forestry machines was measured in field experiments for harvesting and transporting logging residues at forestry operating sites in Japan. In addition, a total audit of energy consumption was undertaken. It involved an assessment of materials, construction, and the repair and maintenance of forestry machines as well as the costs associated with an energy-conversion plant. As a result, the ratio of energy output to input was calculated to be 5.69, indicating that the system examined in this study could be feasible as an energy production system. The CO₂ emission per MWh_e (e: electricity) of the biomass-fired power generation plant was calculated to be 61.8kgCO₂/MWh_e, while that of coal-fired power generation plants in Japan is 960kgCO₂/MWh_e. Therefore, the reduction in the amount of CO₂ emission that would result from replacing coal with biomass for power generation by as much as 3.0 million dry-t/year of logging residues in Japan was estimated to be 1.66 million tCO₂/year, corresponding to 0.142% of the national CO₂ emission. This study provides evidence that Japan could reduce its domestic CO₂ emission by using logging residues as alternative energy resources.     

海南霸王岭热带山地雨林森林循环与群落特征研究

以海南霸王岭1hm^2热带山地雨林的网格样方调查数据为基础，首先对全林分的群落特征进行了分析，然后以森林循环理论为指导，研究了热带山地雨林的种群与群落特征。结果表明：热带山地雨林中不同生活型的树种在利用垂直和水平生态资源上是相对分离的，绝大部分的灌木或小乔木树种的水平生态位宽度较大，垂直生态位宽度较小，中、大乔木则正好相反，而那些低密度、稀有种的生态位宽度最小。随森林循环过程的进行，各树种在不同阶段斑块内出现的频度也会发生相应的变化。霸王岭热带山地雨林中占优势的乔灌木树种大都呈聚集分布的格局，个体数中等的树种大都呈均匀分布的格局，而那些个体数少的低密度种则大都呈随机分布的格局。随着森林循环过程的推进，每个树种所拥有的个体数和所占据的面积是逐渐减少的。森林循环不同阶段内的树种数随着个体数的增加呈现出逐渐减少的总趋势，尤其是由单个体和双个体所对应的物种数较多，3个以上个体对应的树种数则呈现出明显的下降。不同斑块之间组成相似性的大小在一定程度上表明了热带山地雨林森林循环斑块动态的连续性，相邻阶段斑块之间的相似性大于不相邻阶段斑块。以森林循环阶段为基础计算的生态位宽度表明，热带山地雨林中大部分低密度的树种或只集中分布在森林循环的少数阶段斑块内的树种对不同阶段斑块的生态资源利用较少，而那些在森林循环的不同阶段都出现，且个体数或断面积中等的树种，对森林循环不同阶段斑块的生态资源利用较大。     

油茶林生物量与养分生物循环的研究

对不同发育期油茶林生物量与养分的生物循环进行了研究。结果表明，从幼龄期至盛果期油茶林生物量逐步积累，盛果期生物量达到１６．１３９ｔ／ｈｍ２，其中经济生物量为１．７９８ｔ／ｈｍ２；不同发育期油茶林分的生产力不同，盛果期油茶林太阳能利用率最高，为０．２％；不同发育期油茶林光合产物在各器官的分配比例不同；不同发育期油茶林系统大量元素（Ｎ、Ｐ、Ｋ、Ｃａ、Ｍｇ）的吸收量、存留量、归还量及输出量不同。并提出了油茶林系统施肥的监界量。     

Antiproliferative activity of Goldfussia psilostachys ethanolic extract on K562 leukemia cells

A dose-dependent and significant (P<0.01) antiproliferative effect of Goldfussia psilostachys ethanolic extract was observed on K562 cells. The IC(50) is ca. 0.5 microg/ml. The extract markedly increases the proportion of cell in G2/M phases and decreases the population of cells in G0/G1 phases. Moreover, the antimitotic effect is correlated with polymerization of microtubule assembly. These results indicate that G. psilostachys ethanolic extract inhibits the proliferation of K562 cells and disrupts the normal dynamic of microtubules during mitosis.     

林隙(GAP)干扰研究进展

本文系统地介绍了森林循环的理论、自然干扰的概念、林隙的一般特征和林隙干扰的时空格局。干扰是森林循环的驱动力,干扰形成的林隙是森林循环的起点。林隙的大小、形成年龄、形成方式以及形成木的特征是研究林隙特征的重要参数,虽然不同森林群落中,林隙干扰的频率和程度及其时空变化规律不同,但大多数森林的年平均干扰率是在１％左右。不同的森林类型中,大、小型干扰的比例不同。小型林隙干扰与大型灾难性干扰在不同时空条件下的组合,就形成了不同森林景观的时空变化格局。     

miR-21与Nocodazole联合作用对小鼠成肌细胞C2C12周期的影响

观察miR-21与Nocodazole联合作用对小鼠成肌细胞C2C12周期的影响。分别以0、200、300、400、500、600 nmol/L Nocodazole处理C2C12细胞24 h,流式细胞仪检测细胞周期,间接免疫染色激光共聚焦显微镜观察细胞有丝分裂器α-微管蛋白(α-tubulin)排列。转染miR-21 mimics/NC和inhibitors/NC,24 h后,添加400 nmol/L的Nocodazole处理24 h,流式细胞仪检测细胞周期。结果表明:Nocodazole使C2C12细胞同步化的最佳浓度是400 nmol/L;过表达miR-21 mimics之后,与对照组相比,G0/G1,S期细胞百分比极显著增加,G2/M期细胞百分比极显著减少(P0.01);添加抑制剂后,与对照组相比,添加抑制剂(inhibitors)的C2C12细胞中处于G0/G1期的细胞比例显著高于对照组,而处于G2/M期的细胞比例显著低于对照组细胞(P0.05);正反试验结果证明,miR-21促进C2C12细胞周期进入S期。为进一步研究miR-21对C2C12细胞的作用机制奠定基础。